The toxicity of benzene was attributed to the formation of free radical. In this study, a method of measuring lipid peroxidation(LPO)in white blood cells(WBC)was reported. After the rats inhaled benzene in different c...The toxicity of benzene was attributed to the formation of free radical. In this study, a method of measuring lipid peroxidation(LPO)in white blood cells(WBC)was reported. After the rats inhaled benzene in different concentrations (6.310\+9, 71.810\+9 and 459.510\+\{-9\} g/L) for 30 days and in concentration of 71.810\+\{-9\} g/L for 10, 20 and 30 days,indicators reflecting toxicity of blood free radicals such as leukocyte LPO, plasma LPO, erythrocyte superoxide dismutase(SOD), erythrocyte glutathione were measured. After 30 day administration, level of leukocyte LPO in all rats of the exposed groups was remarkably higher than that in the control group. Leukopenia occurred only in 71.810\+\{-9\} g/L and 459.510\+\{-9\} g/L groups. The level of leukocyte LPO also increased in 71.810\+\{-9\} g/L group 20 day administration group. Other biochemical indicators had no significant change. It was suggested that the decrease of peripheral leukocytes following benzene exposure might result from, at least in lipid peroxidation, toxic \{effectpart, membrane lipid peroxidation and the determination of leukocyte LPO level is more sensitive than leukocyte count. \;展开更多
文摘The toxicity of benzene was attributed to the formation of free radical. In this study, a method of measuring lipid peroxidation(LPO)in white blood cells(WBC)was reported. After the rats inhaled benzene in different concentrations (6.310\+9, 71.810\+9 and 459.510\+\{-9\} g/L) for 30 days and in concentration of 71.810\+\{-9\} g/L for 10, 20 and 30 days,indicators reflecting toxicity of blood free radicals such as leukocyte LPO, plasma LPO, erythrocyte superoxide dismutase(SOD), erythrocyte glutathione were measured. After 30 day administration, level of leukocyte LPO in all rats of the exposed groups was remarkably higher than that in the control group. Leukopenia occurred only in 71.810\+\{-9\} g/L and 459.510\+\{-9\} g/L groups. The level of leukocyte LPO also increased in 71.810\+\{-9\} g/L group 20 day administration group. Other biochemical indicators had no significant change. It was suggested that the decrease of peripheral leukocytes following benzene exposure might result from, at least in lipid peroxidation, toxic \{effectpart, membrane lipid peroxidation and the determination of leukocyte LPO level is more sensitive than leukocyte count. \;
