The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 5...The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 540-1,244 nucleotides of M fragment of Hantaan virusThen the amplified PCR products were sequenced directlyThe sequencing results demonstrated that there was 84% identity between sample 613 and HV114 virus strain,but was 99% between sample 613 and HTN76-118 strainHowever,in sample 226,there was 95% sequence identity with HV114,82% with HTN76-118The results of phylogenetic tree analysis showed that HV613 was located in the same linage with HTN76-118,the HV226 was in the same linage with HV114 and A9展开更多
文摘The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 540-1,244 nucleotides of M fragment of Hantaan virusThen the amplified PCR products were sequenced directlyThe sequencing results demonstrated that there was 84% identity between sample 613 and HV114 virus strain,but was 99% between sample 613 and HTN76-118 strainHowever,in sample 226,there was 95% sequence identity with HV114,82% with HTN76-118The results of phylogenetic tree analysis showed that HV613 was located in the same linage with HTN76-118,the HV226 was in the same linage with HV114 and A9
