GRB2相关结合蛋白(GRB2-associated binding protein 2,GAB2)是Gabs家族重要成员,在细胞增殖、分化及迁移等过程中发挥重要作用。已有研究证明,GAB2与肿瘤侵袭转移相关,但目前GAB2在乳腺癌细胞侵袭中的研究少见报道。本研究证明,GAB2可...GRB2相关结合蛋白(GRB2-associated binding protein 2,GAB2)是Gabs家族重要成员,在细胞增殖、分化及迁移等过程中发挥重要作用。已有研究证明,GAB2与肿瘤侵袭转移相关,但目前GAB2在乳腺癌细胞侵袭中的研究少见报道。本研究证明,GAB2可提高乳腺癌MCF-7细胞迁移能力。Western印迹结果显示,GAB2蛋白在高转移细胞株MDA-MB-231中高表达;相反,在低转移细胞株MCF-7中低表达。瞬时转染结合Western印迹、酶联免疫吸附实验(ELISA)揭示,过表达GAB2明显增加MMP-2及MMP-9蛋白表达。Transwell检测显示,过表达GAB2明显增强MCF-7细胞的迁移能力。与未转染细胞比较,采用10 ng/m L表皮细胞生长因子(EGF)刺激转染细胞5 min,即可明显增强磷酸化的Akt、ARK5表达。上述结果提示,GAB2可通过参与Akt-ARK5信号通路,增加MMP-2、MMP-9蛋白表达,增强肿瘤细胞的迁移能力,这可能是GAB2促进肿瘤侵袭的部分机制。展开更多
JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world. Addr...JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world. Address manuscripts to the Editorial Office, Journal of Microbiology and Immunology, 4 Sanjianfang Nanli, Chaoyang District, Beijing 100024, China. Manuscripts. Manuscripts are received on the understanding that they have not been published previously in English and are not under simultaneous consideration for another publication. The manuscript, of which the data should be reliable,展开更多
JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world and s...JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world and should be sent to the Editorial Office, Journal of Microbiology and Immunology, 4 Sanjianfang Nanli,Chaoyangqu, Beijing 100024, China.展开更多
基于问题的学习(Problem Based Learning,PBL)模式广泛应用于国外的医学教学上,在PBL模式的启发下,我们在医学微生物学的教学实践中精心设计病例讨论课,学生在讨论中充分发挥主观能动性,通过讨论启发了学生的思维,并提高了学生分析问题...基于问题的学习(Problem Based Learning,PBL)模式广泛应用于国外的医学教学上,在PBL模式的启发下,我们在医学微生物学的教学实践中精心设计病例讨论课,学生在讨论中充分发挥主观能动性,通过讨论启发了学生的思维,并提高了学生分析问题和解决问题的能力,取得了令人满意的效果.展开更多
文摘JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world. Address manuscripts to the Editorial Office, Journal of Microbiology and Immunology, 4 Sanjianfang Nanli, Chaoyang District, Beijing 100024, China. Manuscripts. Manuscripts are received on the understanding that they have not been published previously in English and are not under simultaneous consideration for another publication. The manuscript, of which the data should be reliable,
文摘JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY is an academic, professional periodical published quarterly by the CHINESE MEDICAL ASSOCIATION and distributed worldwide. Manuscripts are welcome from any part of the world and should be sent to the Editorial Office, Journal of Microbiology and Immunology, 4 Sanjianfang Nanli,Chaoyangqu, Beijing 100024, China.
