目的探讨可溶性糖基化终末产物受体(soluble receptor for advanced glycation end products,sRAGE)对小鼠心肌缺血/再灌注(ischemia-reperfusion,I/R)状态下心肌组织蛋白的变化及功能的影响。方法利用心肌细胞特异性过表达sRAGE小鼠,建...目的探讨可溶性糖基化终末产物受体(soluble receptor for advanced glycation end products,sRAGE)对小鼠心肌缺血/再灌注(ischemia-reperfusion,I/R)状态下心肌组织蛋白的变化及功能的影响。方法利用心肌细胞特异性过表达sRAGE小鼠,建立I/R模型,应用蛋白质组学方法检测心肌组织蛋白表达种类及浓度。结果与对照组(I/R+sRAGE KI^(fl/fl))相比较,心肌细胞特异性过表达sRAGE小鼠心肌I/R组(I/R+sRAGE-CKI)心肌组织中上调的蛋白数目是59个,下调的蛋白数目是42个,火山图显示显著上调的蛋白分别是lghg1、lgh2b、Mcm7和Nifk,显著下调的蛋白分别是Abca7、Colla2、Ablim1、Crebrf和Kcp;亚细胞定位结果显示显著变化的蛋白主要分布于细胞核、细胞质、细胞外、质膜、内质网、细胞骨架及其他;功能富集结果显示显著变化的蛋白主要参与调控信号转导、细胞活力、代谢、感染性疾病、肿瘤和免疫系统等过程。结论心肌I/R状态下sRAGE可通过升高或降低参与调控细胞内外信号转导的靶蛋白浓度而抑制心肌I/R损伤。展开更多
目的:探讨TCD无创评估颈动脉重度狭窄及闭塞患者颅内侧支循环开放的血流动力学特征及其与临床预后的相关性。方法:采用回顾性队列研究设计,纳入2021年3月至2024年3月收治的96例单侧颈内动脉重度狭窄(≥70%)或闭塞患者,根据ASITN/SIR侧...目的:探讨TCD无创评估颈动脉重度狭窄及闭塞患者颅内侧支循环开放的血流动力学特征及其与临床预后的相关性。方法:采用回顾性队列研究设计,纳入2021年3月至2024年3月收治的96例单侧颈内动脉重度狭窄(≥70%)或闭塞患者,根据ASITN/SIR侧支评分分为良好代偿组(58例)与不良代偿组(38例)。通过TCD检测大脑中动脉血流动力学参数(PSV、EDV、MFV、PI、RI),结合DSA验证侧支循环状态,随访12个月评估预后。采用Logistic回归分析预后危险因素。结果:良好代偿组PSV(82.32±18.51 vs. 56.45±15.32 cm/s)、MFV(56.93±12.33 vs. 35.89±9.83 cm/s)显著高于不良代偿组,PI(0.64±0.15 vs. 0.82±0.21)、RI(0.43±0.08vs. 0.51±0.11)显著降低(均P<0.001)。TCD参数与ASITN/SIR分级呈强相关性(PSV:r=0.62;PI:r=-0.61,均P<0.001)。多因素分析显示,年龄≥65岁(OR=1.06)、高血压(OR=3.07)、PI≥1.00(OR=17.25)及PSV≤60 cm/s(OR=1.96)为预后不良的独立危险因素(均P<0.05)。结论:TCD多参数联合模型可量化评估侧支循环功能,其中PI≥1.00及PSV≤60 cm/s是预测不良预后的关键指标。TCD无创、动态监测的优势为颈动脉狭窄/闭塞患者的血流动力学分层管理及个体化干预提供了重要依据。展开更多
Hydrogen sulfide (H 2S) may be endogenously produced by cystathionine β lyase (CBS) and cystathionine γ lyase (CSE) as a cardiovascular physiological functional factor. On the hypoxic pulmonary hypertension (HPH) an...Hydrogen sulfide (H 2S) may be endogenously produced by cystathionine β lyase (CBS) and cystathionine γ lyase (CSE) as a cardiovascular physiological functional factor. On the hypoxic pulmonary hypertension (HPH) animal model, the plasma H 2S concentration, the gene expression and the activity (CSE) were decreased in lung tissues In L -NAME induced hypertension and spontaneous hypertension rats (SHR) models, the plasma H 2S concentration, vascular CSE activity and mRNA expression were obviously decreased. When H 2S was exogenously supplied, systolic pressure obviously decrease. These studies suggested that CSE/H 2S pathway participated in the pathophysiological development of hypertension. The endogenous level of H 2S produced by some arterial tissues increased in both septic and endotoxic shock rats. The level of H 2S highly correlated with the endogenous level of NO These results suggest that H 2S may be a novel cardiovascular functional regulator.展开更多
文摘目的探讨可溶性糖基化终末产物受体(soluble receptor for advanced glycation end products,sRAGE)对小鼠心肌缺血/再灌注(ischemia-reperfusion,I/R)状态下心肌组织蛋白的变化及功能的影响。方法利用心肌细胞特异性过表达sRAGE小鼠,建立I/R模型,应用蛋白质组学方法检测心肌组织蛋白表达种类及浓度。结果与对照组(I/R+sRAGE KI^(fl/fl))相比较,心肌细胞特异性过表达sRAGE小鼠心肌I/R组(I/R+sRAGE-CKI)心肌组织中上调的蛋白数目是59个,下调的蛋白数目是42个,火山图显示显著上调的蛋白分别是lghg1、lgh2b、Mcm7和Nifk,显著下调的蛋白分别是Abca7、Colla2、Ablim1、Crebrf和Kcp;亚细胞定位结果显示显著变化的蛋白主要分布于细胞核、细胞质、细胞外、质膜、内质网、细胞骨架及其他;功能富集结果显示显著变化的蛋白主要参与调控信号转导、细胞活力、代谢、感染性疾病、肿瘤和免疫系统等过程。结论心肌I/R状态下sRAGE可通过升高或降低参与调控细胞内外信号转导的靶蛋白浓度而抑制心肌I/R损伤。
文摘目的:探讨TCD无创评估颈动脉重度狭窄及闭塞患者颅内侧支循环开放的血流动力学特征及其与临床预后的相关性。方法:采用回顾性队列研究设计,纳入2021年3月至2024年3月收治的96例单侧颈内动脉重度狭窄(≥70%)或闭塞患者,根据ASITN/SIR侧支评分分为良好代偿组(58例)与不良代偿组(38例)。通过TCD检测大脑中动脉血流动力学参数(PSV、EDV、MFV、PI、RI),结合DSA验证侧支循环状态,随访12个月评估预后。采用Logistic回归分析预后危险因素。结果:良好代偿组PSV(82.32±18.51 vs. 56.45±15.32 cm/s)、MFV(56.93±12.33 vs. 35.89±9.83 cm/s)显著高于不良代偿组,PI(0.64±0.15 vs. 0.82±0.21)、RI(0.43±0.08vs. 0.51±0.11)显著降低(均P<0.001)。TCD参数与ASITN/SIR分级呈强相关性(PSV:r=0.62;PI:r=-0.61,均P<0.001)。多因素分析显示,年龄≥65岁(OR=1.06)、高血压(OR=3.07)、PI≥1.00(OR=17.25)及PSV≤60 cm/s(OR=1.96)为预后不良的独立危险因素(均P<0.05)。结论:TCD多参数联合模型可量化评估侧支循环功能,其中PI≥1.00及PSV≤60 cm/s是预测不良预后的关键指标。TCD无创、动态监测的优势为颈动脉狭窄/闭塞患者的血流动力学分层管理及个体化干预提供了重要依据。
文摘Hydrogen sulfide (H 2S) may be endogenously produced by cystathionine β lyase (CBS) and cystathionine γ lyase (CSE) as a cardiovascular physiological functional factor. On the hypoxic pulmonary hypertension (HPH) animal model, the plasma H 2S concentration, the gene expression and the activity (CSE) were decreased in lung tissues In L -NAME induced hypertension and spontaneous hypertension rats (SHR) models, the plasma H 2S concentration, vascular CSE activity and mRNA expression were obviously decreased. When H 2S was exogenously supplied, systolic pressure obviously decrease. These studies suggested that CSE/H 2S pathway participated in the pathophysiological development of hypertension. The endogenous level of H 2S produced by some arterial tissues increased in both septic and endotoxic shock rats. The level of H 2S highly correlated with the endogenous level of NO These results suggest that H 2S may be a novel cardiovascular functional regulator.
