背景:铜死亡作为一种新兴的细胞死亡方式,近年来受到广泛关注。然而,目前对于铜死亡的全球研究态势,尤其是在不同国家和学术群体中的研究重点与发展趋势尚缺乏系统性的认识。为了更全面地把握铜死亡领域的研究进展,亟需进行多数据库、...背景:铜死亡作为一种新兴的细胞死亡方式,近年来受到广泛关注。然而,目前对于铜死亡的全球研究态势,尤其是在不同国家和学术群体中的研究重点与发展趋势尚缺乏系统性的认识。为了更全面地把握铜死亡领域的研究进展,亟需进行多数据库、多角度的文献计量分析。目的:通过文献计量学方法系统分析铜死亡领域的研究现状、热点及发展趋势。方法:采用文献计量学方法,结合CiteSpace和VOSviewer可视化分析工具,分别对国际权威的Web of Science核心合集数据库与中国知网数据库(CNKI)中2022-2024年间的1378篇铜死亡相关文献进行分析,包括年发文量、科研合作网络、关键词共现聚类与热点变动特征以及高被引文献。通过对比分析这两个数据库的数据,旨在揭示全球视野下铜死亡研究的整体态势。结果与结论:铜死亡研究呈现快速增长趋势,中国发文量居首,以上海交通大学、中南大学等为核心机构。研究主题集中于铜死亡机制、肿瘤治疗应用(化学动力学疗法)及与铁死亡的交叉调控。关键词突现分析表明“免疫微环境”“肿瘤治疗”是研究热点。机器学习基因预测模型和单细胞测序技术正在推动精准医学发展。英文研究多集中于机制探索和技术应用,其研究方法、技术路线和热点方向对正处于快速发展期的国内铜死亡研究具有重要的借鉴意义。尤其是在肿瘤治疗等重大疾病领域开展跨学科合作和多中心临床转化研究,有望为包括中国人群在内的恶性肿瘤等重大疾病诊疗提供更具针对性和有效性的新策略。展开更多
BACKGROUND Mesenchymal stem cells(MSCs)are considered a promising therapy for various diseases due to their strong potential in regenerative medicine and immunomodulation.The tissue source of MSCs has gained attention...BACKGROUND Mesenchymal stem cells(MSCs)are considered a promising therapy for various diseases due to their strong potential in regenerative medicine and immunomodulation.The tissue source of MSCs has gained attention for its role in influencing their function,accessibility,and readiness for clinical use.AIM To identify the most suitable adipose source for MSC isolation and expansion for further applications.METHODS We isolated MSCs from solid adipose tissue and liposuction aspirates using the enzyme method.The MSCs were examined for their expansion using population doubling time,differentiation capacity using multilineage differentiation induction,surface markers using flow cytometry,and stability of chromosomes using the karyotyping method.Growth factors and cytokines in MSC-conditioned media were analyzed using the Luminex assay.RESULTS MSCs were isolated from solid adipose tissue and lipoaspirates and expanded from passage 0 to passage 2.All adipose-derived MSCs(AD-MSCs)exhibited the typical elongated,spindle-shaped morphology and comparable proliferation rate.They expressed positive surface markers(cluster of differentiation 73[CD73]:>97%,CD90:>98%,and CD105:>95%),and negative markers(<1%).All MSCs expressed similar levels of stemness genes(octamer-binding transcription factor 4,SRY-box 2,Krüppel-like factor,and MYC),colonyforming,and trilineage differentiation potential.Karyotyping analysis revealed normal chromosomal patterns in all samples,except one sample exhibiting a polymorphism(1qh+).Furthermore,the growth factors and cytokines of hepatocyte growth factor,vascular endothelial growth factor A,interleukin 6(IL-6),and IL-8 were detected in all AD-MSC conditioned media;but fibroblast growth factor-2 and keratinocyte growth factor were selectively expressed in conditioned media from solid or lipoaspirate AD-MSCs,respectively.CONCLUSION These findings indicate that AD-MSCs from both adipose sources possess all of the characteristic features of MSCs with source-specific secretome differences,which are suitable for further expansion and various clinical applications.展开更多
线粒体电子传递链(Electron transport chain,ETC)通过四个酶复合体将电子从还原供体传递到氧气,同时驱动质子跨内膜转运,形成质子梯度,F1F0 ATP合酶利用该梯度合成ATP,完成细胞能量转换。在此过程中,有少量电子泄漏出ETC从而产生了活性...线粒体电子传递链(Electron transport chain,ETC)通过四个酶复合体将电子从还原供体传递到氧气,同时驱动质子跨内膜转运,形成质子梯度,F1F0 ATP合酶利用该梯度合成ATP,完成细胞能量转换。在此过程中,有少量电子泄漏出ETC从而产生了活性氧(Reactive oxygen species,ROS)。ROS在干细胞的增殖、分化及自我更新中发挥着重要的生理作用,因此ROS水平的精确调控对干细胞的稳态与功能至关重要。研究ETC如何通过ROS的生成来调控干细胞的命运,对于深入理解干细胞的调控机制具有重要意义。本文围绕ETC复合体的结构功能、线粒体ROS产生及其调控干细胞命运机制进行综述,探讨该机制不仅为氧自由基研究提供了新视角,也为临床治疗肿瘤、衰老、神经退行性疾病以及干细胞功能相关疾病提供潜在的治疗靶点。展开更多
文摘背景:铜死亡作为一种新兴的细胞死亡方式,近年来受到广泛关注。然而,目前对于铜死亡的全球研究态势,尤其是在不同国家和学术群体中的研究重点与发展趋势尚缺乏系统性的认识。为了更全面地把握铜死亡领域的研究进展,亟需进行多数据库、多角度的文献计量分析。目的:通过文献计量学方法系统分析铜死亡领域的研究现状、热点及发展趋势。方法:采用文献计量学方法,结合CiteSpace和VOSviewer可视化分析工具,分别对国际权威的Web of Science核心合集数据库与中国知网数据库(CNKI)中2022-2024年间的1378篇铜死亡相关文献进行分析,包括年发文量、科研合作网络、关键词共现聚类与热点变动特征以及高被引文献。通过对比分析这两个数据库的数据,旨在揭示全球视野下铜死亡研究的整体态势。结果与结论:铜死亡研究呈现快速增长趋势,中国发文量居首,以上海交通大学、中南大学等为核心机构。研究主题集中于铜死亡机制、肿瘤治疗应用(化学动力学疗法)及与铁死亡的交叉调控。关键词突现分析表明“免疫微环境”“肿瘤治疗”是研究热点。机器学习基因预测模型和单细胞测序技术正在推动精准医学发展。英文研究多集中于机制探索和技术应用,其研究方法、技术路线和热点方向对正处于快速发展期的国内铜死亡研究具有重要的借鉴意义。尤其是在肿瘤治疗等重大疾病领域开展跨学科合作和多中心临床转化研究,有望为包括中国人群在内的恶性肿瘤等重大疾病诊疗提供更具针对性和有效性的新策略。
文摘BACKGROUND Mesenchymal stem cells(MSCs)are considered a promising therapy for various diseases due to their strong potential in regenerative medicine and immunomodulation.The tissue source of MSCs has gained attention for its role in influencing their function,accessibility,and readiness for clinical use.AIM To identify the most suitable adipose source for MSC isolation and expansion for further applications.METHODS We isolated MSCs from solid adipose tissue and liposuction aspirates using the enzyme method.The MSCs were examined for their expansion using population doubling time,differentiation capacity using multilineage differentiation induction,surface markers using flow cytometry,and stability of chromosomes using the karyotyping method.Growth factors and cytokines in MSC-conditioned media were analyzed using the Luminex assay.RESULTS MSCs were isolated from solid adipose tissue and lipoaspirates and expanded from passage 0 to passage 2.All adipose-derived MSCs(AD-MSCs)exhibited the typical elongated,spindle-shaped morphology and comparable proliferation rate.They expressed positive surface markers(cluster of differentiation 73[CD73]:>97%,CD90:>98%,and CD105:>95%),and negative markers(<1%).All MSCs expressed similar levels of stemness genes(octamer-binding transcription factor 4,SRY-box 2,Krüppel-like factor,and MYC),colonyforming,and trilineage differentiation potential.Karyotyping analysis revealed normal chromosomal patterns in all samples,except one sample exhibiting a polymorphism(1qh+).Furthermore,the growth factors and cytokines of hepatocyte growth factor,vascular endothelial growth factor A,interleukin 6(IL-6),and IL-8 were detected in all AD-MSC conditioned media;but fibroblast growth factor-2 and keratinocyte growth factor were selectively expressed in conditioned media from solid or lipoaspirate AD-MSCs,respectively.CONCLUSION These findings indicate that AD-MSCs from both adipose sources possess all of the characteristic features of MSCs with source-specific secretome differences,which are suitable for further expansion and various clinical applications.
