Single-cell biomechanics and electrophysiology measuring tools have transformed biological research over the last few decades,which enabling a comprehensive and nuanced understanding of cellular behavior and function....Single-cell biomechanics and electrophysiology measuring tools have transformed biological research over the last few decades,which enabling a comprehensive and nuanced understanding of cellular behavior and function.Despite their high-quality information content,these single-cell measuring techniques suffer from laborious manual processing by highly skilled workers and extremely low throughput(tens of cells per day).Recently,numerous researchers have automated the measurement of cell mechanical and electrical signals through robotic localization and control processes.While these efforts have demonstrated promising progress,critical challenges persist,including human dependency,learning complexity,in-situ measurement,and multidimensional signal acquisition.To identify key limitations and highlight emerging opportunities for innovation,in this review,we comprehensively summarize the key steps of robotic technologies in single-cell biomechanics and electrophysiology.We also discussed the prospects and challenges of robotics and automation in biological research.By bridging gaps between engineering,biology,and data science,this work aims to stimulate interdisciplinary research and accelerate the translation of robotic single-cell technologies into practical applications in the life sciences and medical fields.展开更多
通过检索PubMed、Web of Science、中国知网和万方等数据库,对近年来发表的有关扁平足在生物力学上的成因和治疗方法的相关内容进行综述。其中,扁平足的形成原因包括先天性因素和后天性因素,而治疗方法主要有矫形鞋垫、物理疗法、手术...通过检索PubMed、Web of Science、中国知网和万方等数据库,对近年来发表的有关扁平足在生物力学上的成因和治疗方法的相关内容进行综述。其中,扁平足的形成原因包括先天性因素和后天性因素,而治疗方法主要有矫形鞋垫、物理疗法、手术治疗和运动疗法。这些方法在一定程度上能够纠正足部结构异常,优化生物力学性能,提高足部的稳定性和活动范围。对于未来的研究,将利用先进的影像和仿真技术进行精确的病情评估,新型材料和非手术疗法的应用将能够长期缓解症状,而基因研究将推动早期诊断和个性化治疗的发展,智能矫形器的使用将实现实时监测和动态调整,从而为扁平足的评估、干预和治疗提供更多更有效的方法和手段。展开更多
目的 探讨经颅直流电刺激(transcranial direct current stimulation,tDCS)结合抗阻训练对大学生完成引体向上成绩的影响作用,并从神经肌肉活动控制角度探讨训练干预产生作用的潜在机制。方法 25名男性大学生志愿者随机分为tDCS结合抗...目的 探讨经颅直流电刺激(transcranial direct current stimulation,tDCS)结合抗阻训练对大学生完成引体向上成绩的影响作用,并从神经肌肉活动控制角度探讨训练干预产生作用的潜在机制。方法 25名男性大学生志愿者随机分为tDCS结合抗阻训练组(试验组)和单纯抗阻训练组(对照组),12名对照组受试者接受持续8周、每周3次、每次4组、每组12次动作重复的高位下拉力量训练干预,13名试验组受试者在对照组所采用的训练基础上,在每次训练前进行20 min的tDCS干预。在训练干预前后分别对受试者进行高位下拉静态自主最大收缩力(maximal voluntary contraction,MVC)、80%一次最大重复(one repetition maximum,1RM)负荷高位下拉最大重复次数和常规引体向上动作测试。在引体向上动作测试过程中记录上肢主要用力肌肉的表面肌电信号。结果 训练干预后,试验组和对照组引体向上完成次数分别提高了1.74倍和1.42倍,两组受试者MVC和80%1RM负荷高位下拉最大重复次数也都显著提升,但两组受试者上述指标差异皆无统计学意义。训练后两组受试者主动肌肱桡肌、三角肌后束、胸大肌的激活水平皆显著下降。此外,试验组受试者在训练后拮抗肌肱三头肌的共激活水平由0.50±0.22显著下降到0.37±0.09,而对照组在干预前后无显著变化。结论 持续8周的tDCS结合抗阻训练和单纯抗阻训练显著提升大学生引体向上成绩,可能与两种训练皆可以显著提升主动的肌肉收缩能力有关。tDCS结合抗阻训练可以更有效地降低引体向上动作过程中肱三头肌的共激活水平,提高肘关节肌肉的收缩效率。展开更多
基金the National Natural Science Foundation of China[62525301,62127811,62433019]the New Cornerstone Science Foundation through the XPLORER PRIZEthe financial support by the China Postdoctoral Science Foundation[GZB20240797].
文摘Single-cell biomechanics and electrophysiology measuring tools have transformed biological research over the last few decades,which enabling a comprehensive and nuanced understanding of cellular behavior and function.Despite their high-quality information content,these single-cell measuring techniques suffer from laborious manual processing by highly skilled workers and extremely low throughput(tens of cells per day).Recently,numerous researchers have automated the measurement of cell mechanical and electrical signals through robotic localization and control processes.While these efforts have demonstrated promising progress,critical challenges persist,including human dependency,learning complexity,in-situ measurement,and multidimensional signal acquisition.To identify key limitations and highlight emerging opportunities for innovation,in this review,we comprehensively summarize the key steps of robotic technologies in single-cell biomechanics and electrophysiology.We also discussed the prospects and challenges of robotics and automation in biological research.By bridging gaps between engineering,biology,and data science,this work aims to stimulate interdisciplinary research and accelerate the translation of robotic single-cell technologies into practical applications in the life sciences and medical fields.
文摘通过检索PubMed、Web of Science、中国知网和万方等数据库,对近年来发表的有关扁平足在生物力学上的成因和治疗方法的相关内容进行综述。其中,扁平足的形成原因包括先天性因素和后天性因素,而治疗方法主要有矫形鞋垫、物理疗法、手术治疗和运动疗法。这些方法在一定程度上能够纠正足部结构异常,优化生物力学性能,提高足部的稳定性和活动范围。对于未来的研究,将利用先进的影像和仿真技术进行精确的病情评估,新型材料和非手术疗法的应用将能够长期缓解症状,而基因研究将推动早期诊断和个性化治疗的发展,智能矫形器的使用将实现实时监测和动态调整,从而为扁平足的评估、干预和治疗提供更多更有效的方法和手段。
