目的:分析国内外结直肠腺瘤(CRA)领域的研究现状、热点及发展趋势。方法:基于Wed of Science核心数据库和中国知网(CNKI)数据库,检索2012年1月1日至2022年12月31日发表的关于CRA的中英文文献,使用CiteSpace对国家、机构、基金、期刊、...目的:分析国内外结直肠腺瘤(CRA)领域的研究现状、热点及发展趋势。方法:基于Wed of Science核心数据库和中国知网(CNKI)数据库,检索2012年1月1日至2022年12月31日发表的关于CRA的中英文文献,使用CiteSpace对国家、机构、基金、期刊、作者、被引文献和关键词进行可视化分析。结果:筛选后共纳入3478篇文献,其中英文文献2579篇,中文文献899篇,发文量最多的国家是美国,中英文文献发文量最高的机构分别是武汉大学人民医院消化内科、美国退伍军人事务部,相关领域研究机构与作者相对分散,跨区域机构合作研究较少,国内外聚焦存在相对差异,国内探索中医辨证治疗可预防或降低CRA复发,国外研究人工智能(AI)辅助结肠镜检查,AI提高CRA检出率、危险因素、预防复发及“腺瘤-癌”序列是未来研究热点。结论:腺瘤检出率、危险因素、复发机制及腺瘤癌变之间的关系是CRA的研究热点和趋势,AI提高腺瘤检测率、中医药预防、降低腺瘤复发及“腺瘤-癌”序列的深入研究是未来的重点探究方向。展开更多
AIM: Ursolic acid (UA) and oleanolic acid (OA) are triperpene acids having a similar chemical structure and are distributed wildly in plants all over the world. In recent years, it was found that they had marked anti-...AIM: Ursolic acid (UA) and oleanolic acid (OA) are triperpene acids having a similar chemical structure and are distributed wildly in plants all over the world. In recent years, it was found that they had marked anti-tumor effects. There is little literature currently available regarding their effects on colon carcinoma cells. The present study was designed to investigate their inhibitory effects on human colon carcinoma cell line HCT15. METHODS: HCT15 cells were cultured with different drugs. The treated cells were stained with hematoxylin-eosin and their morphologic changes observed under a light microscope. The cytotoxicity of these drugs was evaluated by tetrazolium dye assay. Cell cycle analysis was performed by flow cytometry (FCM). Data were expressed as means +/-SEM and Analysis of variance and Student' t-test for individual comparisons. RESULTS: Twenty-four to 72 h after UA or OA 60 micromol/L treatment, the numbers of dead cells and cell fragments were increased and most cells were dead at the 72nd hour. The cytotoxicity of UA was stronger than that of OA. Seventy-eight hours after 30 micromol/L of UA or OA treatment, a number of cells were degenerated, but cell fragments were rarely seen. The IC(50) values for UA and OA were 30 and 60 micromol/L, respectively. Proliferation assay showed that proliferation of UA and OA-treated cells was slightly increased at 24h and significantly decreased at 48 h and 60 h, whereas untreated control cells maintained an exponential growth curve. Cell cycle analysis by FCM showed HCT15 cells treated with UA 30 and OA 60 for 36 h and 72 h gradually accumulated in G(0)/G(1) phase (both drugs P【0.05 for 72 h), with a concomitant decrease of cell populations in S phase (both drugs P【0.01 for 72 h) and no detectable apoptotic fraction. CONCLUSION: UA and OA have significant anti-tumor activity. The effect of UA is stronger than that of OA. The possible mechanism of action is that both drugs have an inhibitory effect on tumor cell proliferation through cell-cycle arrest.展开更多
文摘目的:分析国内外结直肠腺瘤(CRA)领域的研究现状、热点及发展趋势。方法:基于Wed of Science核心数据库和中国知网(CNKI)数据库,检索2012年1月1日至2022年12月31日发表的关于CRA的中英文文献,使用CiteSpace对国家、机构、基金、期刊、作者、被引文献和关键词进行可视化分析。结果:筛选后共纳入3478篇文献,其中英文文献2579篇,中文文献899篇,发文量最多的国家是美国,中英文文献发文量最高的机构分别是武汉大学人民医院消化内科、美国退伍军人事务部,相关领域研究机构与作者相对分散,跨区域机构合作研究较少,国内外聚焦存在相对差异,国内探索中医辨证治疗可预防或降低CRA复发,国外研究人工智能(AI)辅助结肠镜检查,AI提高CRA检出率、危险因素、预防复发及“腺瘤-癌”序列是未来研究热点。结论:腺瘤检出率、危险因素、复发机制及腺瘤癌变之间的关系是CRA的研究热点和趋势,AI提高腺瘤检测率、中医药预防、降低腺瘤复发及“腺瘤-癌”序列的深入研究是未来的重点探究方向。
文摘AIM: Ursolic acid (UA) and oleanolic acid (OA) are triperpene acids having a similar chemical structure and are distributed wildly in plants all over the world. In recent years, it was found that they had marked anti-tumor effects. There is little literature currently available regarding their effects on colon carcinoma cells. The present study was designed to investigate their inhibitory effects on human colon carcinoma cell line HCT15. METHODS: HCT15 cells were cultured with different drugs. The treated cells were stained with hematoxylin-eosin and their morphologic changes observed under a light microscope. The cytotoxicity of these drugs was evaluated by tetrazolium dye assay. Cell cycle analysis was performed by flow cytometry (FCM). Data were expressed as means +/-SEM and Analysis of variance and Student' t-test for individual comparisons. RESULTS: Twenty-four to 72 h after UA or OA 60 micromol/L treatment, the numbers of dead cells and cell fragments were increased and most cells were dead at the 72nd hour. The cytotoxicity of UA was stronger than that of OA. Seventy-eight hours after 30 micromol/L of UA or OA treatment, a number of cells were degenerated, but cell fragments were rarely seen. The IC(50) values for UA and OA were 30 and 60 micromol/L, respectively. Proliferation assay showed that proliferation of UA and OA-treated cells was slightly increased at 24h and significantly decreased at 48 h and 60 h, whereas untreated control cells maintained an exponential growth curve. Cell cycle analysis by FCM showed HCT15 cells treated with UA 30 and OA 60 for 36 h and 72 h gradually accumulated in G(0)/G(1) phase (both drugs P【0.05 for 72 h), with a concomitant decrease of cell populations in S phase (both drugs P【0.01 for 72 h) and no detectable apoptotic fraction. CONCLUSION: UA and OA have significant anti-tumor activity. The effect of UA is stronger than that of OA. The possible mechanism of action is that both drugs have an inhibitory effect on tumor cell proliferation through cell-cycle arrest.
