目的评估中老年人群血清除草剂的暴露水平及其影响因素。方法采用横断面研究设计,数据来源于2008年建立的东风-同济队列研究(汽车公司退休职工)的基线资料,研究对象选自队列中已建立的2型糖尿病巢式病例对照人群,于2021年4月最终纳入201...目的评估中老年人群血清除草剂的暴露水平及其影响因素。方法采用横断面研究设计,数据来源于2008年建立的东风-同济队列研究(汽车公司退休职工)的基线资料,研究对象选自队列中已建立的2型糖尿病巢式病例对照人群,于2021年4月最终纳入2012名无癌症及心血管疾病史且留存充足血液样本可用于农药暴露水平检测的研究对象。使用问卷调查中老年人群的人口学特征和生活方式等信息,通过气相色谱-三重四极杆质谱联用仪检测其血清中异丙甲草胺、苯胺灵和乙草胺等22种除草剂的浓度。采用Spearman秩相关分析血清除草剂之间的相关性,使用非参数检验比较影响因素不同组别之间的差异性,使用多因素回归分析各种影响因素与血清除草剂浓度的关系。结果中老年人群的年龄为(62.8±7.4)岁,其中男性占44.1%(888/2012)。检测的22种除草剂可分为9类,包括三嗪类、酰胺类、二硝基苯胺类、二苯醚类、苯氨基甲酸酯类、环状亚胺类、芳氧苯氧丙酸酯类、三唑类和苯并噁嗪类。其中,异丙甲草胺、苯胺灵和乙草胺的检出率分别为95.33%(1918/2012)、93.74%(1886/2012)和81.46%(1639/2012),其余19种除草剂的检出率低于50%。异丙甲草胺、苯胺灵和乙草胺的中位数浓度分别为0.055、0.157和0.032 ng/ml。相关性分析显示,乙草胺与异丙甲草胺呈正相关(rs=0.35,P<0.001)。单因素分析结果显示,男性血清中苯胺灵的浓度明显低于女性(P<0.05)。多因素回归分析显示,男性血清中异丙甲草胺和苯胺灵的浓度均明显低于女性(P<0.05),体质指数(body mass index,BMI)与血清中异丙甲草胺浓度呈正向关联(P<0.05),蛋类摄入≥3次/周人群血清中苯胺灵浓度明显高于蛋类摄入<3次/周的人群(P<0.05),而乳制品摄入≥5次/周人群血清中苯胺灵浓度明显低于乳制品摄入<5次/周的人群(P<0.05)。结论中老年人群血清中除草剂的水平存在性别差异,BMI和饮食习惯(如蛋类摄入频次增加或乳制品摄入频次减少)可能是中老年人血清除草剂暴露水平相关的影响因素。展开更多
Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male W...Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male Wistar albino rats(Rattus norvegicus)weighing 160-200 g and aged 5-6 months randomly received a single intratesticular injection of normal saline(Group A),zinc gluconate 13.3 mg/mL plus L-Arginine(Group B),20%CaCl_(2)(Group C),and CdCl_(2)0.5 mg/kg body weight plus ethylenediaminetetraacetic acid(EDTA)(Group D),respectively,along the entire route from the caudoventral aspect of each testis.They were euthanized up to 180 days to evaluate reproductive tract toxicology.Results:The reproductive organ weights were markedly reduced,with testes severely atrophied in group B,pea-sized and stony hard in group C,and moderately reduced in group D.Azoospermia was evident in groups B and C,while sperm concentration was reduced to<1 million/mL with zero sperm motility in group D.Rats of groups B and C failed to show mounting and copulatory behaviour.A completely disorganized mesh of cellular elements was observed in the seminiferous tubules of group B,while pyknotic germ cell and arrest of spermatogenesis,exfoliated germ cells,occasional syncytial bodies and smaller Leydig cells were evident in groups C and D.Significantly reduced testosterone levels,increased luteinizing hormone(LH)and follicle-stimulating hormone(FSH)levels,returned to normal after 90 days in group D.Conclusions:Zinc gluconate and CaCl_(2)injections severely affected the reproductive organs and libido and rats treated with CdCl_(2)exhibited diminished spermatogenesis with normal libido.Thus,the need-based selection of intratesticular agents should consider their distinct effects on spermatogenesis,libido,and hormonal balance for achieving targeted sterilization outcomes.展开更多
文摘目的评估中老年人群血清除草剂的暴露水平及其影响因素。方法采用横断面研究设计,数据来源于2008年建立的东风-同济队列研究(汽车公司退休职工)的基线资料,研究对象选自队列中已建立的2型糖尿病巢式病例对照人群,于2021年4月最终纳入2012名无癌症及心血管疾病史且留存充足血液样本可用于农药暴露水平检测的研究对象。使用问卷调查中老年人群的人口学特征和生活方式等信息,通过气相色谱-三重四极杆质谱联用仪检测其血清中异丙甲草胺、苯胺灵和乙草胺等22种除草剂的浓度。采用Spearman秩相关分析血清除草剂之间的相关性,使用非参数检验比较影响因素不同组别之间的差异性,使用多因素回归分析各种影响因素与血清除草剂浓度的关系。结果中老年人群的年龄为(62.8±7.4)岁,其中男性占44.1%(888/2012)。检测的22种除草剂可分为9类,包括三嗪类、酰胺类、二硝基苯胺类、二苯醚类、苯氨基甲酸酯类、环状亚胺类、芳氧苯氧丙酸酯类、三唑类和苯并噁嗪类。其中,异丙甲草胺、苯胺灵和乙草胺的检出率分别为95.33%(1918/2012)、93.74%(1886/2012)和81.46%(1639/2012),其余19种除草剂的检出率低于50%。异丙甲草胺、苯胺灵和乙草胺的中位数浓度分别为0.055、0.157和0.032 ng/ml。相关性分析显示,乙草胺与异丙甲草胺呈正相关(rs=0.35,P<0.001)。单因素分析结果显示,男性血清中苯胺灵的浓度明显低于女性(P<0.05)。多因素回归分析显示,男性血清中异丙甲草胺和苯胺灵的浓度均明显低于女性(P<0.05),体质指数(body mass index,BMI)与血清中异丙甲草胺浓度呈正向关联(P<0.05),蛋类摄入≥3次/周人群血清中苯胺灵浓度明显高于蛋类摄入<3次/周的人群(P<0.05),而乳制品摄入≥5次/周人群血清中苯胺灵浓度明显低于乳制品摄入<5次/周的人群(P<0.05)。结论中老年人群血清中除草剂的水平存在性别差异,BMI和饮食习惯(如蛋类摄入频次增加或乳制品摄入频次减少)可能是中老年人血清除草剂暴露水平相关的影响因素。
基金financially assisted to Timanshi Chansoriya of the fellowship grant of UGC-NFPwD,New Delhi sanctioned vide UGC F.No.01-01/2019-Sch dated 06.11.2020(Serial No.352 ID:NFPWD-2018-20-UTP-6522).
文摘Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male Wistar albino rats(Rattus norvegicus)weighing 160-200 g and aged 5-6 months randomly received a single intratesticular injection of normal saline(Group A),zinc gluconate 13.3 mg/mL plus L-Arginine(Group B),20%CaCl_(2)(Group C),and CdCl_(2)0.5 mg/kg body weight plus ethylenediaminetetraacetic acid(EDTA)(Group D),respectively,along the entire route from the caudoventral aspect of each testis.They were euthanized up to 180 days to evaluate reproductive tract toxicology.Results:The reproductive organ weights were markedly reduced,with testes severely atrophied in group B,pea-sized and stony hard in group C,and moderately reduced in group D.Azoospermia was evident in groups B and C,while sperm concentration was reduced to<1 million/mL with zero sperm motility in group D.Rats of groups B and C failed to show mounting and copulatory behaviour.A completely disorganized mesh of cellular elements was observed in the seminiferous tubules of group B,while pyknotic germ cell and arrest of spermatogenesis,exfoliated germ cells,occasional syncytial bodies and smaller Leydig cells were evident in groups C and D.Significantly reduced testosterone levels,increased luteinizing hormone(LH)and follicle-stimulating hormone(FSH)levels,returned to normal after 90 days in group D.Conclusions:Zinc gluconate and CaCl_(2)injections severely affected the reproductive organs and libido and rats treated with CdCl_(2)exhibited diminished spermatogenesis with normal libido.Thus,the need-based selection of intratesticular agents should consider their distinct effects on spermatogenesis,libido,and hormonal balance for achieving targeted sterilization outcomes.
文摘目的基于网络毒理学、分子对接和分子动力学模拟,探讨全氟辛酸(perfluorooctanoic acid,PFOA)诱发骨质疏松症(osteoporosis,OP)的机制。方法预测PFOA的毒性特征,通过ChEMBL、GeneCards数据库获取PFOA和OP的相关靶点,构建蛋白互作网络。进行基因本体论和京都基因与基因组百科全书富集分析、分子对接与分子动力学模拟。结果共筛选出PFOA诱发OP的40个潜在靶点,其中雌激素受体(estrogen receptor,ESR)1,过氧化物酶体增殖物激活受体γ,基质金属蛋白酶-9,过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptor alpha,PPARA),核受体亚家族1H组成员4(nuclear receptor subfamily 1 group H member 4,NR1H4),ESR2等为核心靶点。富集分析结果显示,靶点显著富集于过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor,PPAR)信号通路和雌激素信号通路。分子对接显示PFOA与ESR1等靶点具有良好的结合亲和力,分子动力学模拟证实PFOA与PPARA、NR1H4和ESR2结合稳定。结论PFOA诱发OP具有多靶点、多通路协同作用的特点,可通过调控PPAR信号通路和雌激素信号通路等影响OP的发生与发展。
