Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male W...Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male Wistar albino rats(Rattus norvegicus)weighing 160-200 g and aged 5-6 months randomly received a single intratesticular injection of normal saline(Group A),zinc gluconate 13.3 mg/mL plus L-Arginine(Group B),20%CaCl_(2)(Group C),and CdCl_(2)0.5 mg/kg body weight plus ethylenediaminetetraacetic acid(EDTA)(Group D),respectively,along the entire route from the caudoventral aspect of each testis.They were euthanized up to 180 days to evaluate reproductive tract toxicology.Results:The reproductive organ weights were markedly reduced,with testes severely atrophied in group B,pea-sized and stony hard in group C,and moderately reduced in group D.Azoospermia was evident in groups B and C,while sperm concentration was reduced to<1 million/mL with zero sperm motility in group D.Rats of groups B and C failed to show mounting and copulatory behaviour.A completely disorganized mesh of cellular elements was observed in the seminiferous tubules of group B,while pyknotic germ cell and arrest of spermatogenesis,exfoliated germ cells,occasional syncytial bodies and smaller Leydig cells were evident in groups C and D.Significantly reduced testosterone levels,increased luteinizing hormone(LH)and follicle-stimulating hormone(FSH)levels,returned to normal after 90 days in group D.Conclusions:Zinc gluconate and CaCl_(2)injections severely affected the reproductive organs and libido and rats treated with CdCl_(2)exhibited diminished spermatogenesis with normal libido.Thus,the need-based selection of intratesticular agents should consider their distinct effects on spermatogenesis,libido,and hormonal balance for achieving targeted sterilization outcomes.展开更多
统计分析Web of Science(WoS)数据库与中国知网(China national knowledge infrastructure,CNKI)数据库中2015—2024年国内外的内分泌干扰物领域研究文献,旨在了解该领域的研究现状,为该领域研究人员提供全球视角。分析结果表明:10年间...统计分析Web of Science(WoS)数据库与中国知网(China national knowledge infrastructure,CNKI)数据库中2015—2024年国内外的内分泌干扰物领域研究文献,旨在了解该领域的研究现状,为该领域研究人员提供全球视角。分析结果表明:10年间两个数据库中发表内分泌干扰物相关文献共9 084篇,Wo S数据库年均发文量超过600篇,CNKI数据库年均发文量约100篇;全球发文机构中,法国国家卫生与医学研究院、法国国家科学研究中心和加利福尼亚大学系统位居前3位,在国内,则是浙江大学、南京大学和天津医科大学;中国、美国和法国位居Wo S数据库发文量的前3位,其中中国和美国发文量均超过1 500篇。展开更多
基金financially assisted to Timanshi Chansoriya of the fellowship grant of UGC-NFPwD,New Delhi sanctioned vide UGC F.No.01-01/2019-Sch dated 06.11.2020(Serial No.352 ID:NFPWD-2018-20-UTP-6522).
文摘Objective:To investigate the sterilizing potential of zinc gluconate,calcium chloride(CaCl_(2))and cadmium chloride(CdCl_(2))following a single intratesticular administration in adult male rats.Methods:60 adult male Wistar albino rats(Rattus norvegicus)weighing 160-200 g and aged 5-6 months randomly received a single intratesticular injection of normal saline(Group A),zinc gluconate 13.3 mg/mL plus L-Arginine(Group B),20%CaCl_(2)(Group C),and CdCl_(2)0.5 mg/kg body weight plus ethylenediaminetetraacetic acid(EDTA)(Group D),respectively,along the entire route from the caudoventral aspect of each testis.They were euthanized up to 180 days to evaluate reproductive tract toxicology.Results:The reproductive organ weights were markedly reduced,with testes severely atrophied in group B,pea-sized and stony hard in group C,and moderately reduced in group D.Azoospermia was evident in groups B and C,while sperm concentration was reduced to<1 million/mL with zero sperm motility in group D.Rats of groups B and C failed to show mounting and copulatory behaviour.A completely disorganized mesh of cellular elements was observed in the seminiferous tubules of group B,while pyknotic germ cell and arrest of spermatogenesis,exfoliated germ cells,occasional syncytial bodies and smaller Leydig cells were evident in groups C and D.Significantly reduced testosterone levels,increased luteinizing hormone(LH)and follicle-stimulating hormone(FSH)levels,returned to normal after 90 days in group D.Conclusions:Zinc gluconate and CaCl_(2)injections severely affected the reproductive organs and libido and rats treated with CdCl_(2)exhibited diminished spermatogenesis with normal libido.Thus,the need-based selection of intratesticular agents should consider their distinct effects on spermatogenesis,libido,and hormonal balance for achieving targeted sterilization outcomes.
文摘目的基于网络毒理学、分子对接和分子动力学模拟,探讨全氟辛酸(perfluorooctanoic acid,PFOA)诱发骨质疏松症(osteoporosis,OP)的机制。方法预测PFOA的毒性特征,通过ChEMBL、GeneCards数据库获取PFOA和OP的相关靶点,构建蛋白互作网络。进行基因本体论和京都基因与基因组百科全书富集分析、分子对接与分子动力学模拟。结果共筛选出PFOA诱发OP的40个潜在靶点,其中雌激素受体(estrogen receptor,ESR)1,过氧化物酶体增殖物激活受体γ,基质金属蛋白酶-9,过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptor alpha,PPARA),核受体亚家族1H组成员4(nuclear receptor subfamily 1 group H member 4,NR1H4),ESR2等为核心靶点。富集分析结果显示,靶点显著富集于过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor,PPAR)信号通路和雌激素信号通路。分子对接显示PFOA与ESR1等靶点具有良好的结合亲和力,分子动力学模拟证实PFOA与PPARA、NR1H4和ESR2结合稳定。结论PFOA诱发OP具有多靶点、多通路协同作用的特点,可通过调控PPAR信号通路和雌激素信号通路等影响OP的发生与发展。
文摘统计分析Web of Science(WoS)数据库与中国知网(China national knowledge infrastructure,CNKI)数据库中2015—2024年国内外的内分泌干扰物领域研究文献,旨在了解该领域的研究现状,为该领域研究人员提供全球视角。分析结果表明:10年间两个数据库中发表内分泌干扰物相关文献共9 084篇,Wo S数据库年均发文量超过600篇,CNKI数据库年均发文量约100篇;全球发文机构中,法国国家卫生与医学研究院、法国国家科学研究中心和加利福尼亚大学系统位居前3位,在国内,则是浙江大学、南京大学和天津医科大学;中国、美国和法国位居Wo S数据库发文量的前3位,其中中国和美国发文量均超过1 500篇。
