Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumul...Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumulation in females.A reduction in juvenile hormone(JH)biosynthesis is known to initiate reproductive diapause,but its regulatory mechanism remains unclear.Seven up(Svp),a transcription factor from the nuclear receptor family,plays a crucial role in various developmental processes in insects.In this study,using the cabbage beetle Colaphellus bowringi as a model,we observed higher expression of Svp in the heads of female adults under reproductive photoperiodic conditions(short-day[SD])compared to diapause conditions(long-day[LD]).RNA interference-mediated knockdown of Svp in SD females induced typical diapause phenotypes,including ovarian arrest and lipid accumulation.The application of methoprene(ME),a JH receptor agonist,reversed these diapause phenotypes and restored reproduction,indicating that Svp’s regulation of reproductive diapause is dependent on JH signaling.Additionally,Svp knockdown led to the downregulation of JH pathway genes and a reduction in JH titers.Further evidence suggested that Svp regulates the expression of JHAMT1,a critical gene in JH biosynthesis,which determines diapause entry in C.bowringi.These findings suggest that diapause-inducing photoperiods suppress Svp expression,blocking JH production and triggering diapause.This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production,providing a potential target for controlling pests capable of entering reproductive diapause.展开更多
Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production...Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production and nutrition supply during development.Here,we identify that the mutation of xgr,a gene encoding an ATPase,results in a reduced body size in Drosophila.Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts.Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level.Glucose reabsorption in the Malpighian tubules is significantly reduced,as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function.Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level,thereby impacting body size.Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.展开更多
基金funded by the National Natural Science Foundation of China,China(grant no.32472543).
文摘Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumulation in females.A reduction in juvenile hormone(JH)biosynthesis is known to initiate reproductive diapause,but its regulatory mechanism remains unclear.Seven up(Svp),a transcription factor from the nuclear receptor family,plays a crucial role in various developmental processes in insects.In this study,using the cabbage beetle Colaphellus bowringi as a model,we observed higher expression of Svp in the heads of female adults under reproductive photoperiodic conditions(short-day[SD])compared to diapause conditions(long-day[LD]).RNA interference-mediated knockdown of Svp in SD females induced typical diapause phenotypes,including ovarian arrest and lipid accumulation.The application of methoprene(ME),a JH receptor agonist,reversed these diapause phenotypes and restored reproduction,indicating that Svp’s regulation of reproductive diapause is dependent on JH signaling.Additionally,Svp knockdown led to the downregulation of JH pathway genes and a reduction in JH titers.Further evidence suggested that Svp regulates the expression of JHAMT1,a critical gene in JH biosynthesis,which determines diapause entry in C.bowringi.These findings suggest that diapause-inducing photoperiods suppress Svp expression,blocking JH production and triggering diapause.This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production,providing a potential target for controlling pests capable of entering reproductive diapause.
基金funded by the National Key R&D Program of China(2021YFA0805800,2023YFE0107700,and 2020YFA0803202 to R.J.),the 111 Project(D18010 to R.J.)the Guangzhou Medical University Discipline Construction Funds(Basic Medicine)(JCXKJS2022A02 to R.J.)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2024A1515010752 to C.W.)the Special Innovation Projects of Universities in Guangdong Province(2022KTSCx096 to C.W.).
文摘Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production and nutrition supply during development.Here,we identify that the mutation of xgr,a gene encoding an ATPase,results in a reduced body size in Drosophila.Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts.Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level.Glucose reabsorption in the Malpighian tubules is significantly reduced,as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function.Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level,thereby impacting body size.Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.
