Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production...Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production and nutrition supply during development.Here,we identify that the mutation of xgr,a gene encoding an ATPase,results in a reduced body size in Drosophila.Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts.Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level.Glucose reabsorption in the Malpighian tubules is significantly reduced,as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function.Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level,thereby impacting body size.Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.展开更多
蜂王浆是深受消费者喜爱的蜂产品之一,王浆主蛋白(major royal jelly proteins,MRJPs)具有调控蜜蜂级型分化和改善人体健康的重要功能。工蜂咽下腺是蜂王浆蛋白质的“生产车间”,其合成和分泌活性直接影响到蜂王浆品质。近年来,不同研...蜂王浆是深受消费者喜爱的蜂产品之一,王浆主蛋白(major royal jelly proteins,MRJPs)具有调控蜜蜂级型分化和改善人体健康的重要功能。工蜂咽下腺是蜂王浆蛋白质的“生产车间”,其合成和分泌活性直接影响到蜂王浆品质。近年来,不同研究在咽下腺形态结构、转录组水平和蛋白组水平揭示了蜂种、发育阶段和环境条件调控MRJPs合成与分泌的分子基础,为提升蜂王浆品质提供理论基础。本文总结了MRJPs组成和功能,围绕蜜蜂咽下腺和影响MRJPs合成和分泌的因素进行了综述,并对探究蜜蜂咽下腺合成和分泌MRJPs的研究方向进行了展望:(1)深入了解咽下腺合成MRJPs的内在机理;(2)绘制咽下腺合成MRJPs的作用路径;(3)从细胞层面剖析咽下腺合成MRJPs的关键细胞类型及功能。以期为开展MRJPs合成机制相关的研究提供新视角。展开更多
基金funded by the National Key R&D Program of China(2021YFA0805800,2023YFE0107700,and 2020YFA0803202 to R.J.),the 111 Project(D18010 to R.J.)the Guangzhou Medical University Discipline Construction Funds(Basic Medicine)(JCXKJS2022A02 to R.J.)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2024A1515010752 to C.W.)the Special Innovation Projects of Universities in Guangdong Province(2022KTSCx096 to C.W.).
文摘Body size control is fundamental to development and requires proper energy engagement.One of the key energy sensing factors is AMP-activated protein kinase(AMPK),which regulates glucose uptake to ensure ATP production and nutrition supply during development.Here,we identify that the mutation of xgr,a gene encoding an ATPase,results in a reduced body size in Drosophila.Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts.Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level.Glucose reabsorption in the Malpighian tubules is significantly reduced,as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function.Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level,thereby impacting body size.Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.
文摘蜂王浆是深受消费者喜爱的蜂产品之一,王浆主蛋白(major royal jelly proteins,MRJPs)具有调控蜜蜂级型分化和改善人体健康的重要功能。工蜂咽下腺是蜂王浆蛋白质的“生产车间”,其合成和分泌活性直接影响到蜂王浆品质。近年来,不同研究在咽下腺形态结构、转录组水平和蛋白组水平揭示了蜂种、发育阶段和环境条件调控MRJPs合成与分泌的分子基础,为提升蜂王浆品质提供理论基础。本文总结了MRJPs组成和功能,围绕蜜蜂咽下腺和影响MRJPs合成和分泌的因素进行了综述,并对探究蜜蜂咽下腺合成和分泌MRJPs的研究方向进行了展望:(1)深入了解咽下腺合成MRJPs的内在机理;(2)绘制咽下腺合成MRJPs的作用路径;(3)从细胞层面剖析咽下腺合成MRJPs的关键细胞类型及功能。以期为开展MRJPs合成机制相关的研究提供新视角。
