Insects are the most diverse group on earth,partially owing to their metamorphosis and strong fecundity.Circular RNAs(circRNAs)are stable molecules implicated in a broad range of biological processes.However,the regul...Insects are the most diverse group on earth,partially owing to their metamorphosis and strong fecundity.Circular RNAs(circRNAs)are stable molecules implicated in a broad range of biological processes.However,the regulatory roles of circRNAs in insect metamorphosis and reproduction are unclear.Methoprene-tolerant(Met)is the nuclear receptor of juvenile hormone(JH)that plays dual roles,inhibiting precocious metamorphosis and promoting reproduction.Here,we report that locust Met generates two circRNAs,circMet1 and circMet2,respectively.While circMet1 is highly expressed in the cuticle of late final instar,circMet2 is more abundant in the corpora allata,brain,and fat body of early vitellogenic adults.Interestingly,circMet2 is generated by complementary pairing of Penelope-like remnants across the introns of Met.Moreover,circMet2 functions as a miRNA sponge of four species-specific miRNAs that downregulate Met translation.siRNA-mediated knockdown of circMet1 causes the delay of metamorphosis and retarded vitellogenesis.Loss of circMet2 results in significantly decreased vitellogenin synthesis,along with blocked ovarian growth.These results reveal the differential roles of circMet1 and circMet2 in modulating insect metamorphosis and female reproduction.This study advances our understanding of how circRNAs derived from a single gene exert distinct roles in insect life history.展开更多
The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionar...The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionary research.Here,we sequenced the mitochondrial genome of Ornithophila metallica(Schiner,1864)for the first time.It is 15,739 bp in length and possesses 37 mitochondrial genes,comprising 13 protein-coding genes(PCGs),22 tRNA and two rRNA genes,and a non-coding control region.The gene arrangement is consistent with that of most Dipteran mitochondrial genomes.The mitogenome is subsequently used for phylogeny reconstruction using all available mitogenomes of Hippoboscoidea.The Hippoboscidae is recovered as a monophyletic group,sister to the clade of(streblid+Nycteribiidae),which is the sister group to the monophyletic Glossinidae.Within the Hippoboscidae,the Ornithophila clusters together with Ornithomya,which is a sister group to(Melophagus+Lipoptena).This study broadens our knowledge of the Hippoboscidae and contributes to research in entomology,veterinary science,and ecology.展开更多
Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial ge...Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial genome of A.sinica.The results showed that the complete mitogenome is 15,600 bp in length and had a high AT content(76.30%),with a typical set of 37 genes,including 13 protein-coding genes(PCGs),22 transfer RNA genes(tRNAs),2 ribosomal RNA genes(rRNAs),and 1 control region(CR)Based on the sequences of 13PCGs+2rRNAs,the phylogenetic analysis revealed that A.sinica,Coniopteryx sp.,Conwentzia sinica Yang,1974,Semidalis macleodi Meinander,1972 and Semidalis aleyrodiformis Stephens,1836 form a clade,which is a sister group to other families of Neuroptera.展开更多
基金supported by the National Natural Science Foundation of China(32070489 and U22A20482)the National Key R&D Program of China(2022YFD1400500).
文摘Insects are the most diverse group on earth,partially owing to their metamorphosis and strong fecundity.Circular RNAs(circRNAs)are stable molecules implicated in a broad range of biological processes.However,the regulatory roles of circRNAs in insect metamorphosis and reproduction are unclear.Methoprene-tolerant(Met)is the nuclear receptor of juvenile hormone(JH)that plays dual roles,inhibiting precocious metamorphosis and promoting reproduction.Here,we report that locust Met generates two circRNAs,circMet1 and circMet2,respectively.While circMet1 is highly expressed in the cuticle of late final instar,circMet2 is more abundant in the corpora allata,brain,and fat body of early vitellogenic adults.Interestingly,circMet2 is generated by complementary pairing of Penelope-like remnants across the introns of Met.Moreover,circMet2 functions as a miRNA sponge of four species-specific miRNAs that downregulate Met translation.siRNA-mediated knockdown of circMet1 causes the delay of metamorphosis and retarded vitellogenesis.Loss of circMet2 results in significantly decreased vitellogenin synthesis,along with blocked ovarian growth.These results reveal the differential roles of circMet1 and circMet2 in modulating insect metamorphosis and female reproduction.This study advances our understanding of how circRNAs derived from a single gene exert distinct roles in insect life history.
基金funded by the National Natural Science Foundation of China(3220036532170450)the Fundamental Research Funds for the Central Universities(JCYJ202511).
文摘The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionary research.Here,we sequenced the mitochondrial genome of Ornithophila metallica(Schiner,1864)for the first time.It is 15,739 bp in length and possesses 37 mitochondrial genes,comprising 13 protein-coding genes(PCGs),22 tRNA and two rRNA genes,and a non-coding control region.The gene arrangement is consistent with that of most Dipteran mitochondrial genomes.The mitogenome is subsequently used for phylogeny reconstruction using all available mitogenomes of Hippoboscoidea.The Hippoboscidae is recovered as a monophyletic group,sister to the clade of(streblid+Nycteribiidae),which is the sister group to the monophyletic Glossinidae.Within the Hippoboscidae,the Ornithophila clusters together with Ornithomya,which is a sister group to(Melophagus+Lipoptena).This study broadens our knowledge of the Hippoboscidae and contributes to research in entomology,veterinary science,and ecology.
基金supported by Beijing Natural Science Foundation(5224042).
文摘Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial genome of A.sinica.The results showed that the complete mitogenome is 15,600 bp in length and had a high AT content(76.30%),with a typical set of 37 genes,including 13 protein-coding genes(PCGs),22 transfer RNA genes(tRNAs),2 ribosomal RNA genes(rRNAs),and 1 control region(CR)Based on the sequences of 13PCGs+2rRNAs,the phylogenetic analysis revealed that A.sinica,Coniopteryx sp.,Conwentzia sinica Yang,1974,Semidalis macleodi Meinander,1972 and Semidalis aleyrodiformis Stephens,1836 form a clade,which is a sister group to other families of Neuroptera.
