设置养分贮存损失状态不同的4类培养液,分别添加一定浓度梯度的L-谷氨酰胺(L-G lu tam ine,L-G ln)培养大鼠前体脂肪细胞。通过形态学观察、M TT比色、油红O染色提取法,比较各组细胞形态及增殖与分化的效果。结果表明,添加0 mm o l/L L-...设置养分贮存损失状态不同的4类培养液,分别添加一定浓度梯度的L-谷氨酰胺(L-G lu tam ine,L-G ln)培养大鼠前体脂肪细胞。通过形态学观察、M TT比色、油红O染色提取法,比较各组细胞形态及增殖与分化的效果。结果表明,添加0 mm o l/L L-G ln的新鲜培养液和配制后4℃冷藏20 d,再添加0.685 mm o l/L L-G ln的培养液,均较利于前体脂肪细胞的增殖与分化;配制后4℃冷藏90 d的培养液,对前体脂肪细胞培养效果较差,添加L-G ln也不能使培养效果得到改善。另外,启封后的M 199和胎牛血清(F eta l C a lf Serum,FCS)分别冷藏不宜超过90 d。说明及时满足培养液中L-G ln的需求量,可使前体脂肪细胞达到较好的增殖与分化状态。展开更多
The objective of this research was to develop the causal relationship model of firm characteristics factors and good corporate governance affecting the performance of the companies listed on the Stock Exchange of Thai...The objective of this research was to develop the causal relationship model of firm characteristics factors and good corporate governance affecting the performance of the companies listed on the Stock Exchange of Thailand. The developed model consisted of three latent variables and eight observable variables. The sample of this study was 338 companies listed on the Stock Exchange of Thailand. Data are from annual reports （form-56）, annual financial statements and notes to the fmancial statements during the years 2010-2013. The statistics used in this study were descriptive statistics. The causal model was analyzed through a path analysis with LISREL 8.80 Student Edition. The results showed as follows. By testing the consistence of the causal model of finn characteristics （CHA）, the assumed model was consistent with empirical data. Based on the statistical test, the Chi-Square was 22.08. The statistical probability （p） was 0.077, the degree of freedom （dO was to 0, 2/2 was 1.58, RMSEA was 0.041, SRMR was 0.037, GFI was 0.98, CFI was 0.99, AGFI was 0.96. The latent variable of firm characteristics （CHA） had directly negative impact on performance （PAL） with a statistical significance level of 0.01. A path coefficient was 0.18. However, CHA variable did not indirectly influence performance （PAL） and it had directly negative impact on good corporate governance （GGN） with a path coefficient of 0.02 without statistical significance level. The latent variable of good corporate governance （GGN） had directly positive impact on the performance （PAL） with a statistical significance level of 0.05, with a path coefficient of 0.18.展开更多
文摘设置养分贮存损失状态不同的4类培养液,分别添加一定浓度梯度的L-谷氨酰胺(L-G lu tam ine,L-G ln)培养大鼠前体脂肪细胞。通过形态学观察、M TT比色、油红O染色提取法,比较各组细胞形态及增殖与分化的效果。结果表明,添加0 mm o l/L L-G ln的新鲜培养液和配制后4℃冷藏20 d,再添加0.685 mm o l/L L-G ln的培养液,均较利于前体脂肪细胞的增殖与分化;配制后4℃冷藏90 d的培养液,对前体脂肪细胞培养效果较差,添加L-G ln也不能使培养效果得到改善。另外,启封后的M 199和胎牛血清(F eta l C a lf Serum,FCS)分别冷藏不宜超过90 d。说明及时满足培养液中L-G ln的需求量,可使前体脂肪细胞达到较好的增殖与分化状态。
文摘The objective of this research was to develop the causal relationship model of firm characteristics factors and good corporate governance affecting the performance of the companies listed on the Stock Exchange of Thailand. The developed model consisted of three latent variables and eight observable variables. The sample of this study was 338 companies listed on the Stock Exchange of Thailand. Data are from annual reports （form-56）, annual financial statements and notes to the fmancial statements during the years 2010-2013. The statistics used in this study were descriptive statistics. The causal model was analyzed through a path analysis with LISREL 8.80 Student Edition. The results showed as follows. By testing the consistence of the causal model of finn characteristics （CHA）, the assumed model was consistent with empirical data. Based on the statistical test, the Chi-Square was 22.08. The statistical probability （p） was 0.077, the degree of freedom （dO was to 0, 2/2 was 1.58, RMSEA was 0.041, SRMR was 0.037, GFI was 0.98, CFI was 0.99, AGFI was 0.96. The latent variable of firm characteristics （CHA） had directly negative impact on performance （PAL） with a statistical significance level of 0.01. A path coefficient was 0.18. However, CHA variable did not indirectly influence performance （PAL） and it had directly negative impact on good corporate governance （GGN） with a path coefficient of 0.02 without statistical significance level. The latent variable of good corporate governance （GGN） had directly positive impact on the performance （PAL） with a statistical significance level of 0.05, with a path coefficient of 0.18.
