The International Zebrafish Conference(IZFC)has served as the premier gathering for the zebrafish research community for over 20 years.Featuring numerous plenaries and concurrent sessions,the conference showed the lat...The International Zebrafish Conference(IZFC)has served as the premier gathering for the zebrafish research community for over 20 years.Featuring numerous plenaries and concurrent sessions,the conference showed the latest advancements in zebrafish research and provided attendees with insights into the most innovative research tools and technologies currently available.展开更多
Unraveling the lineage relationships of all descendants from a zygote is fundamental to advancing our understanding of developmental and stem cell biology.However,existing cell barcoding technologies in zebrafish lack...Unraveling the lineage relationships of all descendants from a zygote is fundamental to advancing our understanding of developmental and stem cell biology.However,existing cell barcoding technologies in zebrafish lack the resolution to capture the majority of cell divisions during embryogenesis.A recently developed method,a substitution mutation-aided lineage-tracing system(SMALT),successfully reconstructed high-resolution cell phylogenetic trees for Drosophila melanogaster.Here,we implement the SMALT system in zebrafish,recording a median of 14 substitution mutations on a one-kilobase-pair barcoding sequence for one-day post-fertilization embryos.Leveraging this system,we reconstruct four cell lineage trees for zebrafish fin cells,encompassing both original and regenerated fin.Each tree consists of hundreds of internal nodes with a median bootstrap support of 99%.Analysis of the obtained cell lineage trees reveals that regenerated fin cells mainly originate from cells in the same part of the fins.Through multiple times sampling germ cells from the same individual,we show the stability of the germ cell pool and the early separation of germ cell and somatic cell progenitors.Our system offers the potential for reconstructing high-quality cell phylogenies across diverse tissues,providing valuable insights into development and disease in zebrafish.展开更多
Understanding biological mechanisms is fundamental for improving animal production and health to meet the growing demand for high-quality p rotein.As an emerging biotechnology,single-cell transcriptomics has been grad...Understanding biological mechanisms is fundamental for improving animal production and health to meet the growing demand for high-quality p rotein.As an emerging biotechnology,single-cell transcriptomics has been gradually applied in diverse aspects of animal research,offering an effective method to study the gene expression of highthroughput single cells of different tissues/organs in animals.In an unprecedented manner,researchers have identified cell type s/subtypes and their marker genes,infe rred cellular fate trajectories,and revealed cell-cell in teractions in animals using single-cell transcriptomics.In this paper,we introduce the development of single-cell technology and review the processes,advancements,and applications of single-cell transcriptomics in animal research.We summarize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and healthe reproductive performance,genetics,and disease models in different livestock species.Moreover,the practical experience accumulated based on a large number of cases is highlighted to provide a reference for determining key factors(e.g.,sample size,cell clustering,and cell type annotation)in single-cell transcriptomics analysis.We also discuss the limitations and outlook of single-cell transcriptomics in the current stage.This paper describes the comprehensive progress of single-cell transcriptomics in animal research,offering novel insights and sustainable advancements in agricultural productivity and animal health.展开更多
The specification of germ cells in zebrafish mostly relies on an inherited mechanism by which localized maternal determinants,called germ plasm,confer germline fate in the early embryo.Extensive studies have partially...The specification of germ cells in zebrafish mostly relies on an inherited mechanism by which localized maternal determinants,called germ plasm,confer germline fate in the early embryo.Extensive studies have partially allowed the identification of key regulators governing germ plasm formation and subsequent germ cell development.RNA-binding proteins,acting in concert with other germ plasm components,play essential roles in the organization of the germ plasm and the specification,migration,maintenance,and differentiation of primordial germ cells.The loss of their functions impairs germ cell formation and causes sterility or sexual conversion.Evidence is emerging that they instruct germline development through differential regulation of mRNA fates in somatic and germ cells.However,the challenge remains to decipher the complex interplay of maternal germ plasm components in germ plasm compartmentalization and germ cell specification.Because failure to control the developmental outcome of germ cells disrupts the formation of gametes,it is important to gain a complete picture of regulatory mechanisms operating in the germ cell lineage.This review sheds light on the contributions of RNA-binding proteins to germ cell development in zebrafish and highlights intriguing questions that remain open for future investigation.展开更多
Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from sin...Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from single blastomeres.These cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in vitro.Twenty zebrafish cell lines are successfully established from single blastomeres.Single-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 passages.The long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic analysis.Overall,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.展开更多
Hematopoietic stem and progenitor cells(HsPCs)are a subset population of multi-potent cells with self-renewal capability and differentiation into all lineages of blood cells(Zhang and Liu,2024).HSPCs arise from the ve...Hematopoietic stem and progenitor cells(HsPCs)are a subset population of multi-potent cells with self-renewal capability and differentiation into all lineages of blood cells(Zhang and Liu,2024).HSPCs arise from the ventral wall of embryonic dorsal aorta,the hemogenic endothelium in the aorta-gonad-mesonephros(AGM)region,tightly controlled by epigenetic,transcriptional,and signaling networks(Wu and Hirschi,2021).However,how developmental hemogenic endothelium performs cell fate surveillances during HSPC emergence and efficiently eliminates them to avoid abnormal hematopoiesisremainselusive.展开更多
基金supported by grants from the National Key R&D Program of China(2018YFA0801003,2018YFA0800200,and 2023YFA1800100)the National Natural Science Foundation of China(32030032)the Initiative Scientific Research Program,Institute of Zoology,Chinese Academy of Sciences(20231OZ0305 and 20231OZ0102).
文摘The International Zebrafish Conference(IZFC)has served as the premier gathering for the zebrafish research community for over 20 years.Featuring numerous plenaries and concurrent sessions,the conference showed the latest advancements in zebrafish research and provided attendees with insights into the most innovative research tools and technologies currently available.
基金supported by the National Key R&D Program of China(2021YFA1302500 and 2021YFA1302501)the National Natural Science Foundation of China(32293190,32293191,31970570,and 32200492).
文摘Unraveling the lineage relationships of all descendants from a zygote is fundamental to advancing our understanding of developmental and stem cell biology.However,existing cell barcoding technologies in zebrafish lack the resolution to capture the majority of cell divisions during embryogenesis.A recently developed method,a substitution mutation-aided lineage-tracing system(SMALT),successfully reconstructed high-resolution cell phylogenetic trees for Drosophila melanogaster.Here,we implement the SMALT system in zebrafish,recording a median of 14 substitution mutations on a one-kilobase-pair barcoding sequence for one-day post-fertilization embryos.Leveraging this system,we reconstruct four cell lineage trees for zebrafish fin cells,encompassing both original and regenerated fin.Each tree consists of hundreds of internal nodes with a median bootstrap support of 99%.Analysis of the obtained cell lineage trees reveals that regenerated fin cells mainly originate from cells in the same part of the fins.Through multiple times sampling germ cells from the same individual,we show the stability of the germ cell pool and the early separation of germ cell and somatic cell progenitors.Our system offers the potential for reconstructing high-quality cell phylogenies across diverse tissues,providing valuable insights into development and disease in zebrafish.
基金supported by the Natural Science Foundation of Zhejiang Province Outstanding Youth Fund Project(LR23C170001)。
文摘Understanding biological mechanisms is fundamental for improving animal production and health to meet the growing demand for high-quality p rotein.As an emerging biotechnology,single-cell transcriptomics has been gradually applied in diverse aspects of animal research,offering an effective method to study the gene expression of highthroughput single cells of different tissues/organs in animals.In an unprecedented manner,researchers have identified cell type s/subtypes and their marker genes,infe rred cellular fate trajectories,and revealed cell-cell in teractions in animals using single-cell transcriptomics.In this paper,we introduce the development of single-cell technology and review the processes,advancements,and applications of single-cell transcriptomics in animal research.We summarize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and healthe reproductive performance,genetics,and disease models in different livestock species.Moreover,the practical experience accumulated based on a large number of cases is highlighted to provide a reference for determining key factors(e.g.,sample size,cell clustering,and cell type annotation)in single-cell transcriptomics analysis.We also discuss the limitations and outlook of single-cell transcriptomics in the current stage.This paper describes the comprehensive progress of single-cell transcriptomics in animal research,offering novel insights and sustainable advancements in agricultural productivity and animal health.
文摘The specification of germ cells in zebrafish mostly relies on an inherited mechanism by which localized maternal determinants,called germ plasm,confer germline fate in the early embryo.Extensive studies have partially allowed the identification of key regulators governing germ plasm formation and subsequent germ cell development.RNA-binding proteins,acting in concert with other germ plasm components,play essential roles in the organization of the germ plasm and the specification,migration,maintenance,and differentiation of primordial germ cells.The loss of their functions impairs germ cell formation and causes sterility or sexual conversion.Evidence is emerging that they instruct germline development through differential regulation of mRNA fates in somatic and germ cells.However,the challenge remains to decipher the complex interplay of maternal germ plasm components in germ plasm compartmentalization and germ cell specification.Because failure to control the developmental outcome of germ cells disrupts the formation of gametes,it is important to gain a complete picture of regulatory mechanisms operating in the germ cell lineage.This review sheds light on the contributions of RNA-binding proteins to germ cell development in zebrafish and highlights intriguing questions that remain open for future investigation.
基金supported by the National Key Research and Development Program of China(2018YFA0801003 and 2019YFA0109900)the Center for Excellence in Molecular Cell Science(2021DF06)supported by the Shanghai Municipal Science and Technology Major Project.
文摘Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from single blastomeres.These cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in vitro.Twenty zebrafish cell lines are successfully established from single blastomeres.Single-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 passages.The long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic analysis.Overall,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.
基金supported by the National Key R&D Program of China(2018YFA0800203)the National Natural Science Foundation of China(Distinguished Young Scholars 31925014,Key Program 32130033+1 种基金General Program 31571505)the Key Research Program of Frontier Sciences,Chinese。
文摘Hematopoietic stem and progenitor cells(HsPCs)are a subset population of multi-potent cells with self-renewal capability and differentiation into all lineages of blood cells(Zhang and Liu,2024).HSPCs arise from the ventral wall of embryonic dorsal aorta,the hemogenic endothelium in the aorta-gonad-mesonephros(AGM)region,tightly controlled by epigenetic,transcriptional,and signaling networks(Wu and Hirschi,2021).However,how developmental hemogenic endothelium performs cell fate surveillances during HSPC emergence and efficiently eliminates them to avoid abnormal hematopoiesisremainselusive.
