engrailed基因属于同源异形基因家族成员,在许多动物的分节、附肢发育、神经系统发育和贝壳形成过程中发挥作用。本研究克隆了长牡蛎两个engrailed同源基因,命名为cgi-eng1和cgi-eng2。序列分析表明,两个基因均具备典型engrailed基因保...engrailed基因属于同源异形基因家族成员,在许多动物的分节、附肢发育、神经系统发育和贝壳形成过程中发挥作用。本研究克隆了长牡蛎两个engrailed同源基因,命名为cgi-eng1和cgi-eng2。序列分析表明,两个基因均具备典型engrailed基因保守的5个EH结构域。利用整装原位杂交技术检测了cgi-eng1和cgi-eng2在贝壳形成的关键时期早期D形幼虫时期的表达情况。结果显示,cgi-eng1和cgi-eng2 m RNA高表达于贝壳外缘,可能与早期贝壳形成过程有关。此外,两个基因的在贝壳外缘的表达模式亦有区别,提示两个基因的功能可能存在一定程度的分化。本研究首次系统鉴定了长牡蛎engrailed基因的成员,并发现它们可能均参与幼虫贝壳形成,研究结果有助于加深对贝类早期发育及贝壳形成的理解。展开更多
The phylogenetic relationship among Chinese Parapenaeopsis species was studied by comparing 16S rDNA and COI gene sequence. The results showed that Parapenaeopsis hardwickii(Miers, 1878) and P. cultrirostris Alcock, 1...The phylogenetic relationship among Chinese Parapenaeopsis species was studied by comparing 16S rDNA and COI gene sequence. The results showed that Parapenaeopsis hardwickii(Miers, 1878) and P. cultrirostris Alcock, 1906 identified from Chinese samples were the same species. Parapenaeopsis cornuta(Kishinouye, 1900), P. incisa Liu and Wang, 1987, and P. sinica Liu and Wang, 1987 were different species. However, their systematic relationship were closer than those of other species, and they formed an advanced branch in the phylogenetic trees. Additionally, the systematic position of P. hardwickii(Miers, 1878), P. hungerfordi Alcock, 1905, and P. tenella(Bate, 1888) were not stable in the phylogenetic trees. More genetic markers such as 18S rDNA and 28S rDNA were supposed to be used to confirm the phylogenic relationship of Parapenaeopsis species.展开更多
文摘engrailed基因属于同源异形基因家族成员,在许多动物的分节、附肢发育、神经系统发育和贝壳形成过程中发挥作用。本研究克隆了长牡蛎两个engrailed同源基因,命名为cgi-eng1和cgi-eng2。序列分析表明,两个基因均具备典型engrailed基因保守的5个EH结构域。利用整装原位杂交技术检测了cgi-eng1和cgi-eng2在贝壳形成的关键时期早期D形幼虫时期的表达情况。结果显示,cgi-eng1和cgi-eng2 m RNA高表达于贝壳外缘,可能与早期贝壳形成过程有关。此外,两个基因的在贝壳外缘的表达模式亦有区别,提示两个基因的功能可能存在一定程度的分化。本研究首次系统鉴定了长牡蛎engrailed基因的成员,并发现它们可能均参与幼虫贝壳形成,研究结果有助于加深对贝类早期发育及贝壳形成的理解。
基金supported by the National Natural Science Foundation of China(Nos.31071889 and 30499340)
文摘The phylogenetic relationship among Chinese Parapenaeopsis species was studied by comparing 16S rDNA and COI gene sequence. The results showed that Parapenaeopsis hardwickii(Miers, 1878) and P. cultrirostris Alcock, 1906 identified from Chinese samples were the same species. Parapenaeopsis cornuta(Kishinouye, 1900), P. incisa Liu and Wang, 1987, and P. sinica Liu and Wang, 1987 were different species. However, their systematic relationship were closer than those of other species, and they formed an advanced branch in the phylogenetic trees. Additionally, the systematic position of P. hardwickii(Miers, 1878), P. hungerfordi Alcock, 1905, and P. tenella(Bate, 1888) were not stable in the phylogenetic trees. More genetic markers such as 18S rDNA and 28S rDNA were supposed to be used to confirm the phylogenic relationship of Parapenaeopsis species.
