Root nodule symbiosis(RNS)is a mutualistic association formed between nitrogen-fixing rhizobia or Frankia and host plants limited to four orders within Rosid I―Fabales,Fagales,Cucurbitales,and Rosales―which comprise...Root nodule symbiosis(RNS)is a mutualistic association formed between nitrogen-fixing rhizobia or Frankia and host plants limited to four orders within Rosid I―Fabales,Fagales,Cucurbitales,and Rosales―which comprise the so-called‘Nitrogen Fixing Nodulation Clade’(NFNC).The majority of nodulation studies have focused on Leguminosae,given their agricultural and environmental importance,as well as the widespread occurrence of nodulation among members of this family.Endowing cereal crops with nitrogen fixation,like Leguminosae,presents a strategy to reduce the detrimental effects of synthetic fertilizer overuse.Different hypotheses on the origin of RNS have been proposed;however,key genetic innovations underlying the evolution of RNS,even in Leguminosae,have been rarely reported.In this review,we begin by examining current knowledge of genetic innovations―including gene gain,gene loss,and the acquisition or loss of conserved noncoding sequences(CNS)in preexisting genes.We explore the available evidence supporting these genetic innovations underlying the evolution of RNS in Leguminosae and offer the phylogenomics approach that could be applied to uncover these genetic innovations.Finally,we conclude by proposing a model of genetic innovations underlying the evolution of RNS in Leguminosae and consider the potential implications for the development of nitrogen-fixing crops.展开更多
为系统了解国内外生物固氮的研究现状,本研究基于在Web of Science(WOS)核心合集数据库及中国知网(CNKI)检索的1990—2023年有关生物固氮研究的文献,利用Citespace、VOSviewer、HistCite等文献计量工具,对发文数量、主要国家(地区)与机...为系统了解国内外生物固氮的研究现状,本研究基于在Web of Science(WOS)核心合集数据库及中国知网(CNKI)检索的1990—2023年有关生物固氮研究的文献,利用Citespace、VOSviewer、HistCite等文献计量工具,对发文数量、主要国家(地区)与机构、主要发文期刊和研究学者、重要文献、研究热点以及研究变化趋势等进行了计量分析。结果表明,1)近24年来,生物固氮研究领域发文量呈逐年增多趋势,2010年后中国发文占比显著增多,并成为WOS数据库中生物固氮领域发文量排名第2的国家,且与美国、巴西、德国等发文量较多的国家之间联系密切。植物科学、微生物学是英文发文量较多的学科,生物学、作物学是中文发文量较多的学科,都与农业科学紧密相关。生物固氮相关研究的中英文论文主要发文期刊是《生物技术通报》、《Plant and Soil》、《Soil Biology&Biochemistry》。Hungria M、Urquiaga S、Boddey R M和李友国是该领域发文较多的研究学者。2)生物固氮关键词聚类网络主要可分为生物固氮相关微生物及其定殖,生物固氮对农业生产的影响,生物固氮分子机理,以及生物固氮影响因素等4类,如何提升生物固氮效率并扩大其应用范围成为研究者关注的焦点。3)生物固氮研究近些年的主要挑战包括:扩大根瘤菌宿主范围,提高根际联合固氮效率;将生物固氮引入禾谷类作物中,开发“氮肥自供型”作物;挖掘高效固氮微生物资源,优化菌肥应用技术等。4)随着遗传工程、合成生物学、宏基因组学、转录组学等技术方法的应用以及多学科的交叉研究,更深入探究生物固氮的机理并将其应用到农业生产中,对于减轻农业生产对工业氮肥的依赖,实现绿色低碳农业生产具有重要意义。展开更多
基金supported by the National Natural Science Foundation of China(32300512)and the Xplorer Prize.
文摘Root nodule symbiosis(RNS)is a mutualistic association formed between nitrogen-fixing rhizobia or Frankia and host plants limited to four orders within Rosid I―Fabales,Fagales,Cucurbitales,and Rosales―which comprise the so-called‘Nitrogen Fixing Nodulation Clade’(NFNC).The majority of nodulation studies have focused on Leguminosae,given their agricultural and environmental importance,as well as the widespread occurrence of nodulation among members of this family.Endowing cereal crops with nitrogen fixation,like Leguminosae,presents a strategy to reduce the detrimental effects of synthetic fertilizer overuse.Different hypotheses on the origin of RNS have been proposed;however,key genetic innovations underlying the evolution of RNS,even in Leguminosae,have been rarely reported.In this review,we begin by examining current knowledge of genetic innovations―including gene gain,gene loss,and the acquisition or loss of conserved noncoding sequences(CNS)in preexisting genes.We explore the available evidence supporting these genetic innovations underlying the evolution of RNS in Leguminosae and offer the phylogenomics approach that could be applied to uncover these genetic innovations.Finally,we conclude by proposing a model of genetic innovations underlying the evolution of RNS in Leguminosae and consider the potential implications for the development of nitrogen-fixing crops.
文摘为系统了解国内外生物固氮的研究现状,本研究基于在Web of Science(WOS)核心合集数据库及中国知网(CNKI)检索的1990—2023年有关生物固氮研究的文献,利用Citespace、VOSviewer、HistCite等文献计量工具,对发文数量、主要国家(地区)与机构、主要发文期刊和研究学者、重要文献、研究热点以及研究变化趋势等进行了计量分析。结果表明,1)近24年来,生物固氮研究领域发文量呈逐年增多趋势,2010年后中国发文占比显著增多,并成为WOS数据库中生物固氮领域发文量排名第2的国家,且与美国、巴西、德国等发文量较多的国家之间联系密切。植物科学、微生物学是英文发文量较多的学科,生物学、作物学是中文发文量较多的学科,都与农业科学紧密相关。生物固氮相关研究的中英文论文主要发文期刊是《生物技术通报》、《Plant and Soil》、《Soil Biology&Biochemistry》。Hungria M、Urquiaga S、Boddey R M和李友国是该领域发文较多的研究学者。2)生物固氮关键词聚类网络主要可分为生物固氮相关微生物及其定殖,生物固氮对农业生产的影响,生物固氮分子机理,以及生物固氮影响因素等4类,如何提升生物固氮效率并扩大其应用范围成为研究者关注的焦点。3)生物固氮研究近些年的主要挑战包括:扩大根瘤菌宿主范围,提高根际联合固氮效率;将生物固氮引入禾谷类作物中,开发“氮肥自供型”作物;挖掘高效固氮微生物资源,优化菌肥应用技术等。4)随着遗传工程、合成生物学、宏基因组学、转录组学等技术方法的应用以及多学科的交叉研究,更深入探究生物固氮的机理并将其应用到农业生产中,对于减轻农业生产对工业氮肥的依赖,实现绿色低碳农业生产具有重要意义。
