泛醇-细胞色素C还原酶复合物伴侣(ubiquinol-cytochrome C reductase complex chaperone,BCS1L)主要参与线粒体复合体Ⅲ组装,与线粒体遗传病发病、前列腺癌患者疲劳等有关,然而其在结肠腺癌中的作用尚不清楚。本研究通过整合多组学分析...泛醇-细胞色素C还原酶复合物伴侣(ubiquinol-cytochrome C reductase complex chaperone,BCS1L)主要参与线粒体复合体Ⅲ组装,与线粒体遗传病发病、前列腺癌患者疲劳等有关,然而其在结肠腺癌中的作用尚不清楚。本研究通过整合多组学分析和功能实验,系统探讨了BCS1L在结肠腺癌中的临床意义及分子机制。结果发现,BCS1L在结肠腺癌组织中表达水平显著上调(P<0.05),其高表达与患者不良预后及年龄显著相关(P<0.05)。功能富集结果显示,BCS1L与有机阴离子转运、羧酸转运、有机酸转运等相关信号通路有关。免疫细胞浸润结果显示,BCS1L高表达组中Treg细胞占比增多。突变图谱结果显示,PCLO、ABCA13、LRP1B、FAT3、HYDIN和SOX 9等基因突变频率在BCS1L高表达组显著升高(均P<0.05)。实验验证结果显示,敲低BCS1L能够显著抑制结肠腺癌细胞的增殖能力、克隆形成能力及迁移能力(均P<0.05)。以上研究结果表明,BCS1L可能通过调控物质转运及调节免疫抑制性肿瘤微环境在结肠腺癌细胞生长和迁移过程中发挥关键作用。展开更多
Casein kinase 1(CK1)is an important member of the serine/threonine protein kinase family,playing a crucial role in various cellular processes,including cell cycle regulation,signal transduction,DNA repair,and circadia...Casein kinase 1(CK1)is an important member of the serine/threonine protein kinase family,playing a crucial role in various cellular processes,including cell cycle regulation,signal transduction,DNA repair,and circadian rhythm control.CK1 is also essential in the nervous system,where it regulates neuronal growth,differentiation,and synaptic plasticity.Studies have shown that CK1δ phosphorylates neuron-specific proteins to regulate axonal growth and synaptogenesis.Primary cilia are non-motile microtubule structures present on the surface of most mammalian cells.Recent studies have revealed their multiple roles in cellular physiology and development,and dysfunction of cilia can impact the development and function of the nervous system.CK1 has an important role in the formation and function of primary cilia.By regulating various signaling pathways and the phosphorylation status of proteins,CK1 affects the generation,maintenance,and signaling transduction of cilia.In this review,the relationship between CK1,primary cilia,and the nervous system was explored,focusing on how CK1 influences cilia to regulate the structure and function of the nervous system.展开更多
文摘Casein kinase 1(CK1)is an important member of the serine/threonine protein kinase family,playing a crucial role in various cellular processes,including cell cycle regulation,signal transduction,DNA repair,and circadian rhythm control.CK1 is also essential in the nervous system,where it regulates neuronal growth,differentiation,and synaptic plasticity.Studies have shown that CK1δ phosphorylates neuron-specific proteins to regulate axonal growth and synaptogenesis.Primary cilia are non-motile microtubule structures present on the surface of most mammalian cells.Recent studies have revealed their multiple roles in cellular physiology and development,and dysfunction of cilia can impact the development and function of the nervous system.CK1 has an important role in the formation and function of primary cilia.By regulating various signaling pathways and the phosphorylation status of proteins,CK1 affects the generation,maintenance,and signaling transduction of cilia.In this review,the relationship between CK1,primary cilia,and the nervous system was explored,focusing on how CK1 influences cilia to regulate the structure and function of the nervous system.
