2011年“第二届走向前沿-IFCC国际高级生化和分予细胞生物学暑期培训班”（“The2nd”“Toward cutting-edge-1FCC Advanced Summer School in Biochemistry and Molecular Cell Biology．”于是4-9日在上海余崇山森林宾馆举行，共4460...2011年“第二届走向前沿-IFCC国际高级生化和分予细胞生物学暑期培训班”（“The2nd”“Toward cutting-edge-1FCC Advanced Summer School in Biochemistry and Molecular Cell Biology．”于是4-9日在上海余崇山森林宾馆举行，共4460名学员参加，学员分别来自人陆以及台湾雨和香港科研院所和高校。展开更多
Mitochondrial dysfunction is the key pathogenic mechanism of cerebral injury induced by high-altitude hypoxia.Some Chinese herbal monomers may exert anti-hypoxic effects through enhancing the efficiency of oxidative p...Mitochondrial dysfunction is the key pathogenic mechanism of cerebral injury induced by high-altitude hypoxia.Some Chinese herbal monomers may exert anti-hypoxic effects through enhancing the efficiency of oxidative phosphorylation,in this study,effects of 10 kinds of Chinese herbal monomers on mitochondrial respiration and membrane potential of cerebral mitochondria isolated from hypoxia-exposed rats in vitro were investigated to screen anti-hypoxic drugs.Rats were exposed to a low-pressure environment of 405.35 mm Hg(54.04 kPa)for 3 days to establish high-altitude hypoxic models.Cerebral mitochondria were isolated and treated with different concentrations of Chinese herbal monomers(sinomenine,silymarin,glycyrrhizic acid,baicalin,quercetin,ginkgolide B,saffron,pipedne,ginsenoside Rgl and oxymatrine)for 5 minutes in vitro.Mitochondrial oxygen consumption and membrane potential were measured using a Clark oxygen electrode and the rhodamine 123 fluorescence analysis method,respectively.Hypoxic exposure significantly decreased the state 3 respiratory rate,respiratory control rate and mitochondrial membrane potential,and significantly increased the state 4 respiratory rate.Treatment with saffron ginsenoside Rgl and oxymatrine increased the respiratory control rate in cerebral mitochondria isolated from hypoxia-exposed rats in dose-dependent manners in vitro,while ginsenoside Rgl,piperine and oxymatrine significantly increased the mitochondrial membrane potential in cerebral mitochondria from hypoxia-exposed rats.The Chinese herbal monomers saffron,ginsenoside Rgl piperine and oxymatrine could thus improve cerebral mitochondrial disorders in oxidative phosphorylation induced by hypobaric hypoxia exposure in vitro.展开更多
文摘2011年“第二届走向前沿-IFCC国际高级生化和分予细胞生物学暑期培训班”（“The2nd”“Toward cutting-edge-1FCC Advanced Summer School in Biochemistry and Molecular Cell Biology．”于是4-9日在上海余崇山森林宾馆举行，共4460名学员参加，学员分别来自人陆以及台湾雨和香港科研院所和高校。
基金supported by the Natural Science Foundation of China,No.81171875
文摘Mitochondrial dysfunction is the key pathogenic mechanism of cerebral injury induced by high-altitude hypoxia.Some Chinese herbal monomers may exert anti-hypoxic effects through enhancing the efficiency of oxidative phosphorylation,in this study,effects of 10 kinds of Chinese herbal monomers on mitochondrial respiration and membrane potential of cerebral mitochondria isolated from hypoxia-exposed rats in vitro were investigated to screen anti-hypoxic drugs.Rats were exposed to a low-pressure environment of 405.35 mm Hg(54.04 kPa)for 3 days to establish high-altitude hypoxic models.Cerebral mitochondria were isolated and treated with different concentrations of Chinese herbal monomers(sinomenine,silymarin,glycyrrhizic acid,baicalin,quercetin,ginkgolide B,saffron,pipedne,ginsenoside Rgl and oxymatrine)for 5 minutes in vitro.Mitochondrial oxygen consumption and membrane potential were measured using a Clark oxygen electrode and the rhodamine 123 fluorescence analysis method,respectively.Hypoxic exposure significantly decreased the state 3 respiratory rate,respiratory control rate and mitochondrial membrane potential,and significantly increased the state 4 respiratory rate.Treatment with saffron ginsenoside Rgl and oxymatrine increased the respiratory control rate in cerebral mitochondria isolated from hypoxia-exposed rats in dose-dependent manners in vitro,while ginsenoside Rgl,piperine and oxymatrine significantly increased the mitochondrial membrane potential in cerebral mitochondria from hypoxia-exposed rats.The Chinese herbal monomers saffron,ginsenoside Rgl piperine and oxymatrine could thus improve cerebral mitochondrial disorders in oxidative phosphorylation induced by hypobaric hypoxia exposure in vitro.
