Hematopoiesis is crucial for organismal health,and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with vertebrates.In larvae,hematopoiesis primarily occurs in the lymph gland,wh...Hematopoiesis is crucial for organismal health,and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with vertebrates.In larvae,hematopoiesis primarily occurs in the lymph gland,which contains distinct zones,including the cortical zone,intermediate zone,medullary zone,and posterior signaling center(PSC).Rab1 is vital for membrane trafficking and maintaining the localization of cell adhesion molecules,yet its role in hematopoietic homeostasis is not fully understood.This study investigates the effects of Rab1 dysfunction on β-integrin trafficking within circulating hemocytes and lymph gland cells.Rab1 impairment disrupts the endosomal trafficking of β-integrin,leading to its abnormal localization on cell membranes,which promotes lamellocyte differentiation and alters progenitor dynamics in circulating hemocytes and lymph glands,respectively.We also show that the mislocalization of β-integrin is dependent on the adhesion protein DE-cadherin.The reduction of β-integrin at cell boundaries in PSC cells leads to fewer PSC cells and lamellocyte differentiation.Furthermore,Rab1 regulates the trafficking of β-integrin via the Q-SNARE protein Syntaxin 17(Syx17).Our findings indicate that Rab1 and Syx17 regulate distinct trafficking pathways for β-integrin in different hematopoietic compartments and maintain hematopoietic homeostasis of Drosophila.展开更多
目的评估妊娠期超重肥胖和子代代谢相关(非酒精性)脂肪性肝病(MASLD)发生风险之间的相关性,为生命早期预防MASLD提供理论依据。方法在线检索中国知网、万方数据知识服务平台、中国生物医学文献服务系统SinoMed和PubMed、Embase、Web of ...目的评估妊娠期超重肥胖和子代代谢相关(非酒精性)脂肪性肝病(MASLD)发生风险之间的相关性,为生命早期预防MASLD提供理论依据。方法在线检索中国知网、万方数据知识服务平台、中国生物医学文献服务系统SinoMed和PubMed、Embase、Web of Science、Cochrane Library、PROSPERO、PQDT Global、ScienceDirect 10个数据库中有关妊娠期超重肥胖和子代MASLD发生关联的研究文献,检索时间为2014年1月至2024年12月。由2名研究者独立进行筛选文献、资料提取和质量评价,采用R 4.3.3软件进行统计分析。结果共纳入10篇文献,涉及10229名研究对象,包括4篇队列研究和6篇病例对照研究。队列研究合并效应值显示妊娠期超重肥胖会增加子代MASLD发生风险(RR=1.59,95%CI=1.06~2.39,P<0.05),研究间存在中度异质性(I^(2)=56.9%,P=0.07);病例对照研究合并效应值显示妊娠期超重肥胖与子代MASLD的发生风险呈正相关(OR=2.00,95%CI=1.68~2.39,P<0.05),异质性较低(I^(2)=48.8%,P=0.08)。结论妊娠期超重肥胖与子代MASLD的发生风险呈正相关,孕期体重管理可降低子代MASLD的发生风险。展开更多
基金supported by the National Natural Science Foundation of China(32170484 and 32300384)the Fundamental Research Funds for the Central Universities(2572022DQ07 and 2572020AW04).
文摘Hematopoiesis is crucial for organismal health,and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with vertebrates.In larvae,hematopoiesis primarily occurs in the lymph gland,which contains distinct zones,including the cortical zone,intermediate zone,medullary zone,and posterior signaling center(PSC).Rab1 is vital for membrane trafficking and maintaining the localization of cell adhesion molecules,yet its role in hematopoietic homeostasis is not fully understood.This study investigates the effects of Rab1 dysfunction on β-integrin trafficking within circulating hemocytes and lymph gland cells.Rab1 impairment disrupts the endosomal trafficking of β-integrin,leading to its abnormal localization on cell membranes,which promotes lamellocyte differentiation and alters progenitor dynamics in circulating hemocytes and lymph glands,respectively.We also show that the mislocalization of β-integrin is dependent on the adhesion protein DE-cadherin.The reduction of β-integrin at cell boundaries in PSC cells leads to fewer PSC cells and lamellocyte differentiation.Furthermore,Rab1 regulates the trafficking of β-integrin via the Q-SNARE protein Syntaxin 17(Syx17).Our findings indicate that Rab1 and Syx17 regulate distinct trafficking pathways for β-integrin in different hematopoietic compartments and maintain hematopoietic homeostasis of Drosophila.
