Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face wit...Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face with their intrinsic limitations including metabolic instability and low membrane permeability,the strategies for synthesizing unnatural amino acids and peptides are explored.Among the methods for modifying amino acids and peptides,chemo-and site-selective approaches are preferred because of the ability to fine-tuning structural features.Recently,transition metal-catalyzed Csingle bondH activation has been employed for the functionalization of amino acids and peptides.Through domino Csingle bondH activation/annulation,a series of structurally complex and diverse amino acids and peptides is constructed.This review highlights recent advances in the synthesis of unnatural amino acids and peptides via transition metal-catalyzed Csingle bondH activation/annulation.展开更多
为确定凝胶电泳后条带中DNA的长度和浓度,该文提出了一种通过DNA荧光强度精确分析DNA长度及浓度的有效方法。对D2000 DNA ladder进行凝胶电泳,采集图像并进行处理。利用荧光强度与DNA浓度之间的线性关系以及迁移距离与DNA长度的线性关...为确定凝胶电泳后条带中DNA的长度和浓度,该文提出了一种通过DNA荧光强度精确分析DNA长度及浓度的有效方法。对D2000 DNA ladder进行凝胶电泳,采集图像并进行处理。利用荧光强度与DNA浓度之间的线性关系以及迁移距离与DNA长度的线性关系进行数据分析。结果显示:采用SYBR Green I作为荧光染料电泳DNA时,图像中各像素点中的绿色与红色灰度值可以反映DNA的特征信息,且绿色灰度值明显高于红色灰度值;对于小于2000 bp的DNA片段,其分子量与迁移距离呈反比例函数关系,当二者分别取对数时,其相关系数达0.971,可用于建立DNA分子量与迁移距离的数学模型以计算DNA分子量;通过计算电泳峰峰值及电泳峰积分面积,发现对于浓度比为2∶1的两个DNA条带,二者的比值为2.16与1.96、2.11与1.94、2.16与1.93,表明电泳峰积分面积能更有效地反映DNA的真实浓度值。该研究对于核酸凝胶电泳仪的开发具有重要应用价值。展开更多
肽基水凝胶作为生物活性材料在药学、纳米材料化学、组织工程等领域受到广泛关注。肽水凝胶的设计往往遵循自下而上的思路,即基于分子自组装的方法,以微观结构为基础扩展到宏观结构的形成。这种从微观角度出发的方法缺乏具体的模型阐述...肽基水凝胶作为生物活性材料在药学、纳米材料化学、组织工程等领域受到广泛关注。肽水凝胶的设计往往遵循自下而上的思路,即基于分子自组装的方法,以微观结构为基础扩展到宏观结构的形成。这种从微观角度出发的方法缺乏具体的模型阐述,初学者往往难以上手。因此,我们根据以β-折叠为基本自组装结构的肽基水凝胶的研究,总结了氨基酸对于肽自组装行为以及凝胶结构与应用的影响,期望可以帮助研究者在设计多肽序列时可以尽快地找到切入点,开发出具有特殊功能的肽基水凝胶材料。As bioactive materials, peptide-based hydrogels have attracted extensive attention in the fields of pharmacy, nanomaterial chemistry, tissue engineering, etc. The design of hydrogels often follows the bottom-up approach, that is the method based on molecular self-assembly, extending from microstructure to the formation of macrostructure. But this method from the micro-perspective lacks specific model elaboration, making it difficult to get started for beginners. Therefore, based on some studies on peptide-based hydrogels with β-sheet as the basic self-assembly structure, we summarized the influence of different amino acid sequences on peptide self-assembly behavior, and the structure and application of gel, hoping to help researchers find the entry point as soon as possible when designing peptide sequences, and design and develop peptide-based hydrogel materials with special functions.展开更多
基金supported by the Natural Science Foundation of Jiangsu Province(No.BK20220409)the National Natural Science Foundation of China(No.22401153)+2 种基金the FWO[Fund for Scientific Research-Flanders(Belgium)]for financial support(recipient Erik V.Van der Eycken)the Research Council of the KU Leuven(recipient Erik V.Van der Eycken)the support of the"RUDN University Strategic Academic Leadership Program"(recipient Erik V.Van der Eycken).
文摘Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face with their intrinsic limitations including metabolic instability and low membrane permeability,the strategies for synthesizing unnatural amino acids and peptides are explored.Among the methods for modifying amino acids and peptides,chemo-and site-selective approaches are preferred because of the ability to fine-tuning structural features.Recently,transition metal-catalyzed Csingle bondH activation has been employed for the functionalization of amino acids and peptides.Through domino Csingle bondH activation/annulation,a series of structurally complex and diverse amino acids and peptides is constructed.This review highlights recent advances in the synthesis of unnatural amino acids and peptides via transition metal-catalyzed Csingle bondH activation/annulation.
文摘为确定凝胶电泳后条带中DNA的长度和浓度,该文提出了一种通过DNA荧光强度精确分析DNA长度及浓度的有效方法。对D2000 DNA ladder进行凝胶电泳,采集图像并进行处理。利用荧光强度与DNA浓度之间的线性关系以及迁移距离与DNA长度的线性关系进行数据分析。结果显示:采用SYBR Green I作为荧光染料电泳DNA时,图像中各像素点中的绿色与红色灰度值可以反映DNA的特征信息,且绿色灰度值明显高于红色灰度值;对于小于2000 bp的DNA片段,其分子量与迁移距离呈反比例函数关系,当二者分别取对数时,其相关系数达0.971,可用于建立DNA分子量与迁移距离的数学模型以计算DNA分子量;通过计算电泳峰峰值及电泳峰积分面积,发现对于浓度比为2∶1的两个DNA条带,二者的比值为2.16与1.96、2.11与1.94、2.16与1.93,表明电泳峰积分面积能更有效地反映DNA的真实浓度值。该研究对于核酸凝胶电泳仪的开发具有重要应用价值。
文摘肽基水凝胶作为生物活性材料在药学、纳米材料化学、组织工程等领域受到广泛关注。肽水凝胶的设计往往遵循自下而上的思路,即基于分子自组装的方法,以微观结构为基础扩展到宏观结构的形成。这种从微观角度出发的方法缺乏具体的模型阐述,初学者往往难以上手。因此,我们根据以β-折叠为基本自组装结构的肽基水凝胶的研究,总结了氨基酸对于肽自组装行为以及凝胶结构与应用的影响,期望可以帮助研究者在设计多肽序列时可以尽快地找到切入点,开发出具有特殊功能的肽基水凝胶材料。As bioactive materials, peptide-based hydrogels have attracted extensive attention in the fields of pharmacy, nanomaterial chemistry, tissue engineering, etc. The design of hydrogels often follows the bottom-up approach, that is the method based on molecular self-assembly, extending from microstructure to the formation of macrostructure. But this method from the micro-perspective lacks specific model elaboration, making it difficult to get started for beginners. Therefore, based on some studies on peptide-based hydrogels with β-sheet as the basic self-assembly structure, we summarized the influence of different amino acid sequences on peptide self-assembly behavior, and the structure and application of gel, hoping to help researchers find the entry point as soon as possible when designing peptide sequences, and design and develop peptide-based hydrogel materials with special functions.
