In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission sp...In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission spectrum range:640-740 nm)and coumarin fluorophore(emission spectrum range:440-600 nm)as signal output units,thereby achieving effective spectral separation and highly selective detection of HClO.Under physiological pH conditions,HClO triggers an oxidation-cleavage reaction,releasing methylene blue and coumarin,which emit distinct red and green fluorescence,respectively.This dual-emission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^(-1)(green channel)and 31.55 nmol·L^(-1)(red channel).Furthermore,in cell imaging experiments,this probe demonstrated excellent cell membrane permeability and low cytotoxicity,successfully enabling the monitoring of both endogenous and exogenous HClO in living cells.By incorporating a twochannel self-calibration system,the probe effectively mitigated signal variations caused by instrumental or environmental interference,substantially improving detection sensitivity and reliability.展开更多
The 36CI/CI ratios of The groundwater from Baoding and Cangzhou, Hebei Province have been measured by using the tandem accelerator mass spectrometry. It is concluded that the 36CI ages of the groundwater of the fourth...The 36CI/CI ratios of The groundwater from Baoding and Cangzhou, Hebei Province have been measured by using the tandem accelerator mass spectrometry. It is concluded that the 36CI ages of the groundwater of the fourth group(Q,) near the mountain and the second-third(Q3-Q,) group in the east of the Baoding area are less than 100k4 that the age of the third- fourth(Q,Q1) group in the east of the Baoding area is about 314.5ha and that of the second-third group in the west of The Cangchou area is less than 100ka.展开更多
文摘In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission spectrum range:640-740 nm)and coumarin fluorophore(emission spectrum range:440-600 nm)as signal output units,thereby achieving effective spectral separation and highly selective detection of HClO.Under physiological pH conditions,HClO triggers an oxidation-cleavage reaction,releasing methylene blue and coumarin,which emit distinct red and green fluorescence,respectively.This dual-emission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^(-1)(green channel)and 31.55 nmol·L^(-1)(red channel).Furthermore,in cell imaging experiments,this probe demonstrated excellent cell membrane permeability and low cytotoxicity,successfully enabling the monitoring of both endogenous and exogenous HClO in living cells.By incorporating a twochannel self-calibration system,the probe effectively mitigated signal variations caused by instrumental or environmental interference,substantially improving detection sensitivity and reliability.
文摘The 36CI/CI ratios of The groundwater from Baoding and Cangzhou, Hebei Province have been measured by using the tandem accelerator mass spectrometry. It is concluded that the 36CI ages of the groundwater of the fourth group(Q,) near the mountain and the second-third(Q3-Q,) group in the east of the Baoding area are less than 100k4 that the age of the third- fourth(Q,Q1) group in the east of the Baoding area is about 314.5ha and that of the second-third group in the west of The Cangchou area is less than 100ka.
