目的探讨Er:YAG激光处理早期窝沟龋并用Constic自粘接流动树脂进行预防性充填后的边缘封闭效果。方法收集患有窝沟龋的前磨牙或磨牙60颗,随机分为4组(n=15)。A、B组用传统涡轮手机备洞,C、D组用Er:YAG激光备洞;A、C组酸蚀并涂布粘结剂后...目的探讨Er:YAG激光处理早期窝沟龋并用Constic自粘接流动树脂进行预防性充填后的边缘封闭效果。方法收集患有窝沟龋的前磨牙或磨牙60颗,随机分为4组(n=15)。A、B组用传统涡轮手机备洞,C、D组用Er:YAG激光备洞;A、C组酸蚀并涂布粘结剂后,用F03流体树脂充填;B、D组用Constic自粘接流动树脂直接充填。4组牙经冷热循环后,染色,体式显微镜下拍照,测量渗入深度,并在扫描电镜(scanning electron microscope,SEM)下观察充填体与牙体组织的间隙。结果4组微渗漏程度为B组>A组>D组>C组,C组、D组的微渗漏分别低于A、B组(P<0.05),C组与其它3组间差异均有统计学意义(P<0.05),A组与B、D组间无显著性差异(P>0.05)。SEM观察4组充填体与牙体组织间隙宽度为B组>A组>D组>C组,除A、D组相比无统计学差异(P>0.05)外,其余各组两两相比均有统计学差异。结论Consic自粘接流动树脂抗微渗漏效果较F03流体树脂差,Er:YAG激光备洞能提高Consic自粘接流动树脂预防性树脂充填边缘封闭性。展开更多
Candidatus Liberibacter asiaticus （CaLas）, an uncultured Gram-negative alphaproteobacterium, is the causal agent of Huanglongbing （HLB） in citrus. CaLas resides in phloem sieve tubes and has been shown to be unequ...Candidatus Liberibacter asiaticus （CaLas）, an uncultured Gram-negative alphaproteobacterium, is the causal agent of Huanglongbing （HLB） in citrus. CaLas resides in phloem sieve tubes and has been shown to be unequally distributed in different tissues. Although HLB is a disease of citrus plants, it has been demonstrated that periwinkle can serve as an experimental host of CaLas, which can be transmitted from citrus to periwinkle via the parasitic plant dodder （Cuscuta spp.）. To investigate the distribution of CaLas in various periwinkle tissues, the bacteria were transmitted from an infected periwinkle plant to healthy periwinkles by top-grafting. The movement of the inoculum and associated titer changes were observed over time in various tissues. CaLas could be detected in the leaves, main stems, and roots of infected periwinkle by conventional PCR, and in all three tissues a clear time-dependent change in CaLas titer was observed, with titer increasing soon after inoculation and then decreasing as disease symptoms became severe. The highest titer was found at 25, 35 and 35 days after inoculation in leaves, main stems and roots, respectively. The titer in leaves was much higher than in the main stems and roots at the same time point, and the spatial distribution of CaLas in the leaves, main stems and roots of infected periwinkle was uneven, similar to what has been shown in citrus. The results provide guidance for selecting the proper periwinkle tissues and sampling times for early detection of CaLas.展开更多
We aim to provide a comprehensive overview of the progress in research and development of the reconfigurable intelligent surface(RIS)over the last 2-3 years in this paper,especially when the RIS is used as relays in n...We aim to provide a comprehensive overview of the progress in research and development of the reconfigurable intelligent surface(RIS)over the last 2-3 years in this paper,especially when the RIS is used as relays in next-generation mobile networks.Major areas of re-search in academia are outlined,including fundamental performance,channel estimation,joint optimization with antenna precoding at base stations,propagation channel modeling and meta-material devices of RIS elements.Development in industry is surveyed from the aspects of performance potentials and issues,realistic joint optimization algorithms,control mechanisms,field trials and related activities in standardiza-tion development organizations(SDOs).Our views on how to carry out the engineering-aspect study on RIS for 6G systems are also presented,which cover the realistic performance,the comparison with other topological improvements,approaches for channel modeling,factors for de-signing control mechanisms and the timeline for RIS standardization.展开更多
Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Ch...Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Chinese medicine systems pharmacology(TCMSP)database with its analysis platform for HF as a disease model,the active ingredients of YXTF were filtered according to the oral bioavailability(OB)and druglikeness(DL),and the corresponding targets were predicted.The disease targets of HF were retrieved by Human Phenotype Ontology(HPO)database.Then,“YXTF compounds-Targets-HF”network was constructed in the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database,the network visualized through the Cytoscape software,and the key nodes of the network achieved according to Degree(D),Closeness Centrality(CC),and Betweenness Centrality(BC).Kyoto Encyclopedia of Genes and Genomes(KEGG)gene function enrichment analysis was carried out for the key nodes with the help of GATHER database platform.The rabbit model of HF was established by coarctation of the abdominal aorta and perfusion of propylthiouracil(PTU).The pathological changes and protein expression of transforming growth factor beta(TGF-β)protein were observed by western blotting.Results“YXTF compounds-Targets-HF”network consisted of 403 nodes,136 key nodes and 10 related KEGG pathways that included focal adhesions,cell cycles,mitogen-activated protein kinase(MAPK)signaling pathways,Toll-like receptor signaling pathways,Cytokine-cytokine receptor interactions,Gap junctions,adherens junctions,TGF-β signaling pathways,apoptosis and Wnt signaling pathways,respectively.The expression of TGF-β protein in myocardium decreased significantly.Conclusions According to the network pharmacology,the treatment of YXTF for HF might be related to 10 metabolic pathways.The effects of the multicompound-multitarget-multi pathways and the synergistic effect drawn from the compounds that act as the monarch,minister,adjuvant,guide medicines were revealed through this study.展开更多
文摘目的探讨Er:YAG激光处理早期窝沟龋并用Constic自粘接流动树脂进行预防性充填后的边缘封闭效果。方法收集患有窝沟龋的前磨牙或磨牙60颗,随机分为4组(n=15)。A、B组用传统涡轮手机备洞,C、D组用Er:YAG激光备洞;A、C组酸蚀并涂布粘结剂后,用F03流体树脂充填;B、D组用Constic自粘接流动树脂直接充填。4组牙经冷热循环后,染色,体式显微镜下拍照,测量渗入深度,并在扫描电镜(scanning electron microscope,SEM)下观察充填体与牙体组织的间隙。结果4组微渗漏程度为B组>A组>D组>C组,C组、D组的微渗漏分别低于A、B组(P<0.05),C组与其它3组间差异均有统计学意义(P<0.05),A组与B、D组间无显著性差异(P>0.05)。SEM观察4组充填体与牙体组织间隙宽度为B组>A组>D组>C组,除A、D组相比无统计学差异(P>0.05)外,其余各组两两相比均有统计学差异。结论Consic自粘接流动树脂抗微渗漏效果较F03流体树脂差,Er:YAG激光备洞能提高Consic自粘接流动树脂预防性树脂充填边缘封闭性。
基金supported by the earmarked fund for China Agriculture Research System (CARS-27)the Special Fund for Agro-Scientific Research in the Public Interest, China (2010003067)
文摘Candidatus Liberibacter asiaticus （CaLas）, an uncultured Gram-negative alphaproteobacterium, is the causal agent of Huanglongbing （HLB） in citrus. CaLas resides in phloem sieve tubes and has been shown to be unequally distributed in different tissues. Although HLB is a disease of citrus plants, it has been demonstrated that periwinkle can serve as an experimental host of CaLas, which can be transmitted from citrus to periwinkle via the parasitic plant dodder （Cuscuta spp.）. To investigate the distribution of CaLas in various periwinkle tissues, the bacteria were transmitted from an infected periwinkle plant to healthy periwinkles by top-grafting. The movement of the inoculum and associated titer changes were observed over time in various tissues. CaLas could be detected in the leaves, main stems, and roots of infected periwinkle by conventional PCR, and in all three tissues a clear time-dependent change in CaLas titer was observed, with titer increasing soon after inoculation and then decreasing as disease symptoms became severe. The highest titer was found at 25, 35 and 35 days after inoculation in leaves, main stems and roots, respectively. The titer in leaves was much higher than in the main stems and roots at the same time point, and the spatial distribution of CaLas in the leaves, main stems and roots of infected periwinkle was uneven, similar to what has been shown in citrus. The results provide guidance for selecting the proper periwinkle tissues and sampling times for early detection of CaLas.
文摘We aim to provide a comprehensive overview of the progress in research and development of the reconfigurable intelligent surface(RIS)over the last 2-3 years in this paper,especially when the RIS is used as relays in next-generation mobile networks.Major areas of re-search in academia are outlined,including fundamental performance,channel estimation,joint optimization with antenna precoding at base stations,propagation channel modeling and meta-material devices of RIS elements.Development in industry is surveyed from the aspects of performance potentials and issues,realistic joint optimization algorithms,control mechanisms,field trials and related activities in standardiza-tion development organizations(SDOs).Our views on how to carry out the engineering-aspect study on RIS for 6G systems are also presented,which cover the realistic performance,the comparison with other topological improvements,approaches for channel modeling,factors for de-signing control mechanisms and the timeline for RIS standardization.
基金funding support from the National Natural Science Foundation of China (No. 81673955)the Education Department of Hunan Province (No. 16A158)+1 种基金Domestic First-class Construction Discipline Open Fund Project for Chinese Medicine of Hunan (No. 2018ZYX43)the Postgraduate Innovation Project of Hunan University of Chinese Medicine in 2018 (No. 2018CX43)
文摘Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Chinese medicine systems pharmacology(TCMSP)database with its analysis platform for HF as a disease model,the active ingredients of YXTF were filtered according to the oral bioavailability(OB)and druglikeness(DL),and the corresponding targets were predicted.The disease targets of HF were retrieved by Human Phenotype Ontology(HPO)database.Then,“YXTF compounds-Targets-HF”network was constructed in the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database,the network visualized through the Cytoscape software,and the key nodes of the network achieved according to Degree(D),Closeness Centrality(CC),and Betweenness Centrality(BC).Kyoto Encyclopedia of Genes and Genomes(KEGG)gene function enrichment analysis was carried out for the key nodes with the help of GATHER database platform.The rabbit model of HF was established by coarctation of the abdominal aorta and perfusion of propylthiouracil(PTU).The pathological changes and protein expression of transforming growth factor beta(TGF-β)protein were observed by western blotting.Results“YXTF compounds-Targets-HF”network consisted of 403 nodes,136 key nodes and 10 related KEGG pathways that included focal adhesions,cell cycles,mitogen-activated protein kinase(MAPK)signaling pathways,Toll-like receptor signaling pathways,Cytokine-cytokine receptor interactions,Gap junctions,adherens junctions,TGF-β signaling pathways,apoptosis and Wnt signaling pathways,respectively.The expression of TGF-β protein in myocardium decreased significantly.Conclusions According to the network pharmacology,the treatment of YXTF for HF might be related to 10 metabolic pathways.The effects of the multicompound-multitarget-multi pathways and the synergistic effect drawn from the compounds that act as the monarch,minister,adjuvant,guide medicines were revealed through this study.
