We conducted a field campaign to investigate the chemical composition,sources,and light absorption of submicron aerosols(PM_(1))from early 2022 in Nanjing,China.The average concentration of PM_(1) was 31μg m^(−3),org...We conducted a field campaign to investigate the chemical composition,sources,and light absorption of submicron aerosols(PM_(1))from early 2022 in Nanjing,China.The average concentration of PM_(1) was 31μg m^(−3),organics(33%)constituted the largest fraction,followed by nitrate(30%),sulfate(18%),ammonium(15%),chloride(3%),and rBC(2%).Four organic aerosol(OA)subcomponents were identified,including two primary OA(POA)and two secondary OA(SOA).The less-oxidized SOA(LO-OOA)contributes the most to the total OA mass(59%).LO-OOA is tightly correlated with the tracer ion C_(2)H_(4)O_(2)^(+)from levoglucosan,and another aged biomass-burning derived species,K_(3)SO_(4)^(+),suggesting it was likely influenced by aged biomass-burning OA.Our study also revealed that fireworks during the Spring Festival have a detrimental impact on air quality,contributing to secondary formation and accumulation under static winter meteorological conditions,prolonging the pollution duration.Also,LO-OOA was found to have the strongest light-absorbing ability.Our results highlight that the light absorption of LO-OOA can mainly be attributed to the C_(x)H_(y)N^(+) family,increased with the double-bond equivalent value.The more-oxidized SOA(MO-OOA)exhibited a negligible light absorption and was strongly correlated with daytime photochemical processes,implying a light-bleaching effect.This study enhances our understanding of the regional contribution of biomass combustion and fireworks to PM_(1) pollution in Nanjing,a typical megacity in the Yangtze River Delta region,during winter,aiding in the development of strategies for long-term air quality improvement in the region.展开更多
VOCs(Volatile organic compounds)exert a vital role in ozone and secondary organic aerosol production,necessitating investigations into their concentration,chemical characteristics,and source apportionment for the effe...VOCs(Volatile organic compounds)exert a vital role in ozone and secondary organic aerosol production,necessitating investigations into their concentration,chemical characteristics,and source apportionment for the effective implementation of measures aimed at preventing and controlling atmospheric pollution.FromJuly to October 2020,onlinemonitoringwas conducted in the main urban area of Shijiazhuang to collect data on VOCs and analyze their concentrations and reactivity.Additionally,the PMF(positive matrix factorization)method was utilized to identify the VOCs sources.Results indicated that the TVOCs(total VOCs)concentration was(96.7±63.4μg/m^3),with alkanes exhibiting the highest concentration of(36.1±26.4μg/m^3),followed by OVOCs(16.4±14.4μg/m^3).The key active components were alkenes and aromatics,among which xylene,propylene,toluene,propionaldehyde,acetaldehyde,ethylene,and styrene played crucial roles as reactive species.The sources derived from PMF analysis encompassed vehicle emissions,solvent and coating sources,combustion sources,industrial emissions sources,as well as plant sources,the contribution of which were 37.80%,27.93%,16.57%,15.24%,and 2.46%,respectively.Hence,reducing vehicular exhaust emissions and encouraging neighboring industries to adopt low-volatile organic solvents and coatings should be prioritized to mitigate VOCs levels.展开更多
Ossification of the posterior longitudinal ligament(OPLL)is a degenerative disease characterized by progressive ectopic bone formation process,which can lead to severe neurological impairments and reduced quality of l...Ossification of the posterior longitudinal ligament(OPLL)is a degenerative disease characterized by progressive ectopic bone formation process,which can lead to severe neurological impairments and reduced quality of life.While the etiology of OPLL is generally considered multifactorial,there is no consensus regarding these contributing factors including genetic,endocrine,biomechanical,immune and lifestyle factors.Through accumulating evidence from multidisciplinary investigations,the pathophysiological connection between OPLL and endocrine-metabolic dysregulation is becoming increasingly clear.Nevertheless,comprehensive understanding of the relationship between the two is hindered by several problems,such as methodological limitations and inadequate mechanistic studies.This review takes a deep dive into the possible factors contributing to OPLL from all aspects of metabolism,including glucose metabolism,lipid metabolism,bone and mineral metabolism,leptin,vitamin,growth hormone/IGF-1 and sex hormones,highlighting their potential roles in the onset and progression of OPLL.Clarifying the etiology of OPLL and elucidating the underlying pathogenesis are crucial for advancing both early intervention strategies and therapeutic approaches in clinical management.Therefore,the endocrine and metabolic disorders in OPLL patients should become a focus of future research.展开更多
PM_(2.5) and black carbon(BC)are important air pollutants impacting radiation balance,air quality,health,and ecosystems.Ozone(O_(3))levels are increasing despite decreases in other pollutants,posing a challenge for po...PM_(2.5) and black carbon(BC)are important air pollutants impacting radiation balance,air quality,health,and ecosystems.Ozone(O_(3))levels are increasing despite decreases in other pollutants,posing a challenge for pollution control,especially in coastal cities like Zhoushan,where the monsoonal climate can exacerbate PM_(2.5) and ozone pollution.This study conducted continuous online measurements of major atmospheric pollutants in Zhoushan,Zhejiang Province,in 2020.The results indicate that the highest contribution from local air masses in Zhoushan is observed in spring,accounting for 17.7%,while the greatest average contribution from northern Zhejiang Province,Jiangsu Province,and Shanghai occurs in winter,at 18.5%.Pollutant concentrationswere seasonally variable,with PM_(2.5),BC,and sulfur dioxide concentrations 56.6%,36%,and 58.2%higher in the cold season compared to the warm season.The O_(3) in spring is approximately 50%higher than that in summer.Ship emissions significantly contributed to BC,nitrogen oxides(NO_(x)),and carbon monoxide in Zhoushan.In spring,PM_(2.5) sources included photochemical processes and northern air mass transport,while in winter,PM_(2.5) was due to regional transport.The inhibitory effect of PM_(2.5) on O_(3) formation in the Zhoushan area is relatively weak.Reducing NO_(x) emissions may increase O_(3),emphasizing the need for volatile organic compounds monitoring and regional control measures to improve air quality and ensure sustainable development in Zhoushan.展开更多
HCLS1-associated protein X-1(HAX1)is a multifunctional mitochondrial protein involved in the regulation of apoptosis,a crucial process of programmed cell death,and mRNA processing.Despite its significance,limited stru...HCLS1-associated protein X-1(HAX1)is a multifunctional mitochondrial protein involved in the regulation of apoptosis,a crucial process of programmed cell death,and mRNA processing.Despite its significance,limited structural data is available for HAX1,hindering a comprehensive understanding of its biological function.Notably,the caseinolytic mitochondrial matrix peptidase chaperone subunit B(CLPB)has been identified as an interacting partner of HAX1,yet the biophysical properties and binding affinity governing their interaction remain poorly defined.In this study,we present a thorough biophysical characterization of full-length human HAX1 and CLPB,accomplished through recombinant expression and purification.By employing size exclusion chromatography,dynamic light scattering,and circular dichroism spectroscopy,we successfully established their biophysical properties,revealing contrasting structural features,with CLPB displaying a-helical content and HAX1 exhibiting a disordered nature.Moreover,we employed solutionstate nuclear magnetic resonance(NMR)spectroscopy to probe their binding affinity.Our findings demonstrate the formation of stable multimeric complexes between HAX1 and CLPB,and we quantified a dissociation constant in the low range of micro-molar for their high affinity interaction.These results lay the foundation for further in-depth investigations into the dynamics and energetics governing the HAX1-CLPB interaction,ultimately contributing to a comprehensive understanding of their functional mechanisms.展开更多
As one of the core components of a magnetic refrigerator,magnetic refrigeration materials are expected to have not only a considerable magnetocaloric effect but also excellent thermal conductivity.The poor thermal con...As one of the core components of a magnetic refrigerator,magnetic refrigeration materials are expected to have not only a considerable magnetocaloric effect but also excellent thermal conductivity.The poor thermal conductivity of many competitive oxide-based magnetic refrigerants,exemplified by EuTiO3-based compounds,acts as a major limitation to their practical application.Therefore,improving the thermal conductivity of magnetic refrigeration materials has become a research emphasis of magnetic refrigeration in recent years.In this work,a series of EuTiO_(3)(ETO)/Cu composites with different copper additives was prepared using a solid-phase reaction method by introducing appropriate amounts of copper powder.The influence of the introduction of copper on the phase composition,microstructure,thermal conductivity,and magnetocaloric effect of the composites was systematically investigated.Unexpectedly,the thermal conductivity of the composites is enhanced by up to 260%due to copper addition,accompanied by only a 5%decrease in magnetic entropy change and refrigerating capacity.Copper additive forms localized thermal conductive networks and promotes the densification process,resulting in significantly enhanced thermal conductivity of the composites.This work demonstrates the feasibility of improving the thermal conductivity of oxide-base d magnetic refrigeration materials by introducing highly thermally conductive substances.展开更多
Developing efficient electrocatalysts for oxygen evolution reaction(OER)is imperative to enhance the overall efficiency of electrolysis systems and rechargeable metal-air batteries operating in aqueous solutions.High-...Developing efficient electrocatalysts for oxygen evolution reaction(OER)is imperative to enhance the overall efficiency of electrolysis systems and rechargeable metal-air batteries operating in aqueous solutions.High-entropy materials,featured with their distinctive multi-component properties,have found extensive application as catalysts in electrochemical energy storage and conversion devices.However,synthesizing nanostructured high-entropy compounds under mild conditions poses a significant challenge due to the difficulty in overcoming the immiscibility of multiple metallic constituents.In this context,the current study focuses on the synthesis of an array of nano-sized high entropy sulfides tailored for OER via a facile precursor pyrolysis method at low temperature.The representative compound,Fe Co Ni Cu Mn Sx,demonstrates remarkable OER performance,achieving a current density of 10 m A/cm^(2) at an overpotential of merely 220 m V and excellent stability with constant electrolysis at 100 m A/cm^(2) for over 400 h.The in-situ formed metal(oxy)hydroxide has been confirmed as the real active sites and its exceptional performance can be primarily attributed to the synergistic effects arising from its multiple components.Furthermore,the synthetic methodology presented here is versatile and can be extended to the preparation of high entropy phosphides,which also present favorable OER performance.This research not only introduces promising non-noble electrocatalysts for OER but also offers a facile approach to expand the family of nano high-entropy materials,contributing significantly to the field of electrochemical energy conversion.展开更多
Magnetic refrigeration(MR)technology is regarded as an ideal solution for cryogenic applications,relying on magnetocaloric materials which provide necessary chilling effect.A series of polycrystalline Tm_(1-x)Er_(x)Ni...Magnetic refrigeration(MR)technology is regarded as an ideal solution for cryogenic applications,relying on magnetocaloric materials which provide necessary chilling effect.A series of polycrystalline Tm_(1-x)Er_(x)Ni_(2)Si_(2)(x=0.2,0.4)compounds was synthesized,and their magnetic properties,magnetic phase transition together with magnetocaloric effect(MCE)were studied.The Tm_(1-x)Er_(x)Ni_(2)Si_(2)(x=0.2,0.4)compounds display a field-induced metamagnetic transition from antiferromagnetic(AFM)to ferromagnetism(FM)in excess of 0.2 T,respectively.Meanwhile,the AFM ground state is unstable.Under the field change of 0-2 T,the values of maximal magnetic entropy change(-ΔS_(M)^(max))and refrigerant capacity(RC)for Tm_(0.8)Er_(0.2)Ni_(2)Si_(2)compound are 17.9 J/(kg·K)and 83.5 J/kg,respectively.The large reversible MCE under low magnetic fields(≤2 T)indicates that Tm_(0.8)Er_(0.2)Ni_(2)Si_(2)compound can serve as potential candidate materials for cryogenic magnetic refrigeration.展开更多
Discoidin domain receptors(DDRs)are single-pass transmembrane proteins belonging to receptor tyrosine kinases(RTKs)family,which are activated by collagen ligands with unusual slow,sustained kinetics,distinguishing the...Discoidin domain receptors(DDRs)are single-pass transmembrane proteins belonging to receptor tyrosine kinases(RTKs)family,which are activated by collagen ligands with unusual slow,sustained kinetics,distinguishing them from canonical RTKs.While DDRs play critical roles in cell adhesion,differentiation,and cancer progression,their activation mechanisms remain partly understood.Here,we investigated the transmembrane domains(TMDs)of DDR1 and DDR2 to elucidate their interaction dynamics in membrane.Using bacterial adenylate cyclase two-hybrid(BACTH)assays,we demonstrated robust homotypic interactions and even stronger heterotypic associations between DDRTMDs.NMR spectroscopy of DDR1TMD and DDR2TMD reconstituted in lipid bilayer-mimetic bicelles showed obvious chemical shift alterations,further validating the stability of their heterocomplex formation.Systematic mutagenesis identified leucine zipper motifs rather than GXXXA motifs mediated both homo-and hetero-associations of DDR1TMD and DDR2TMD.These findings demonstrated the TMD as a critical mediator of DDRs oligomerization and revealed their interaction patterns within membrane.Our study advances the understanding of DDR signaling regulation and highlights transmembrane domain interactions as potential targets for modulating DDR-related pathologies.展开更多
Background:Lung cancer is the leading cause of cancer-related mortality,and while low-dose computed tomography screening may reduce mortality,emerging prognostic models show superior discriminative efficacy compared t...Background:Lung cancer is the leading cause of cancer-related mortality,and while low-dose computed tomography screening may reduce mortality,emerging prognostic models show superior discriminative efficacy compared to age-and smoking history-based screening.However,further research is needed to assess their reliability in predicting lung cancer risk in high-risk patients.Methods:This study evaluated the predictive performance and quality of existing lung cancer prognostic models through a systematic review and meta-analysis.A comprehensive search was conducted in PubMed,Cochrane,Web of Science,CNKI,and Wanfang for articles published between January 1,2000,and February 13,2025,identifying population-basedmodels incorporating all available modeling data.Results:Among 72 analyzed studies,models were developed from Asian(28 studies,including 23 Chinese cohorts)and European/American(48 studies)populations,with only 6 focusing on nonsmokers.Twenty-one models included genetic markers,15 used clinical factors,and 40 integrated epidemiological predictors.Although 37 models underwent external validation,only 4 demonstrated minimal bias and clinical applicability.A meta-analysis of 11 repeatedly validated models revealed calibration and discrimination,though some lacked calibration data.Conclusions:Few lung cancer prognostic models exist for nonsmokers.Most models exhibit poor predictive performance in external validations,with significant bias and limited application scope.Widespread external validation,standardized model development,and reporting techniques are needed to accurately identify high-risk individuals and ensure applicability across diverse populations.展开更多
Pressure sensors are essential for a wide range of applica-tions,including health monitoring,industrial diagnostics,etc.However,achieving both high sensitivity and mechanical ability to withstand high pressure in a si...Pressure sensors are essential for a wide range of applica-tions,including health monitoring,industrial diagnostics,etc.However,achieving both high sensitivity and mechanical ability to withstand high pressure in a single material remains a significant challenge.This study introduces a high-performance cellulose hydrogel inspired by the biomi-metic layered porous structure of human skin.The hydrogel features a novel design composed of a soft layer with large macropores and a hard layer with small micropores,each of which contribute uniquely to its pressure-sensing capabilities.The macropores in the soft part facilitate significant deforma-tion and charge accumulation,providing exceptional sensitivity to low pressures.In contrast,the microporous structure in the hard part enhances pressure range,ensuring support under high pressures and preventing structural failure.The performance of hydrogel is further optimized through ion introduction,which improves its conductivity,and as well the sensitivity.The sensor demonstrated a high sensitivity of 1622kPa^(-1),a detec-tion range up to 160 kPa,excellent conductivity of 4.01 Sm^(-1),rapid response time of 33 ms,and a low detection limit of 1.6 Pa,outperforming most existing cellulose-based sensors.This innovative hierarchically porous architecture not only enhances the pressure-sensing performance but also offers a simple and effective approach for utilizing natural polymers in sensing technologies.The cellulose hydrogel demonstrates sig-nificant potential in both health monitoring and industrial applications,providing a sensitive,durable,and versatile solution for pressure sensing.展开更多
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobac...Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.展开更多
An outburst of coal and gas is a major hazard in underground coal mining. It is generally accepted that an outburst occurs when certain conditions of stress, coal gassiness and physical-mechanical properties of coal a...An outburst of coal and gas is a major hazard in underground coal mining. It is generally accepted that an outburst occurs when certain conditions of stress, coal gassiness and physical-mechanical properties of coal are met. Outbursting is recognized as a two-step process, i.e., initiation and development. In this paper, we present a fully-coupled solid and fluid code to model the entire process of an outburst. The deformation, failure and fracture of solid （coal） are modeled with the discrete element method, and the flow of fluid （gas and water） such as free flow and Darcy flow are modeled with the lattice Boltzmann method. These two methods are coupled in a two-way process, i.e., the solid part provides a moving boundary condition and transfers momentum to the fluid, while the fluid exerts a dragging force upon the solid. Gas desorption from coal occurs at the solid-fluid boundary, and gas diffusion is implemented in the solid code where particles are assumed to be porous. A simple 2D example to simulate the process of an outburst with the model is also presented in this paper to demonstrate the capability of the coupled model.展开更多
The ability to navigate long distances is essential for many animals to locate shelter,food,and breeding grounds.Magnetic sense has evolved in various migratory and homing species to orient them based on the geomagnet...The ability to navigate long distances is essential for many animals to locate shelter,food,and breeding grounds.Magnetic sense has evolved in various migratory and homing species to orient them based on the geomagnetic field.A highly conserved ironsulfur cluster assembly protein IscA is proposed as an animal magnetoreceptor(MagR).Iron-sulfur cluster binding is also suggested to play an essential role in MagR magnetism and is thus critical in animal magnetoreception.In the current study,we provide evidence for distinct iron binding and iron-sulfur cluster binding in MagR in pigeons,an avian species that relies on the geomagnetic field for navigation and homing.Pigeon MagR showed significantly higher total iron content from both iron-and ironsulfur binding.Y65 in pigeon MagR was shown to directly mediate mononuclear iron binding,and its mutation abolished iron-binding capacity of the protein.Surprisingly,both iron binding and iron-sulfur binding demonstrated synergistic effects,and thus appear to be integral and indispensable to pigeon MagR magnetism.These results not only extend our current understanding of the origin and complexity of MagR magnetism,but also imply a possible molecular explanation for the huge diversity in animal magnetoreception.展开更多
Wuhan National High Magnetic Field Center(WHMFC)at Huazhong University of Science and Technology is one of the top-class research centers in the world,which can offer pulsed fields up to 90.6 T with different field wa...Wuhan National High Magnetic Field Center(WHMFC)at Huazhong University of Science and Technology is one of the top-class research centers in the world,which can offer pulsed fields up to 90.6 T with different field waveforms for scientific research and has passed the final evaluation of the Chinese government in 2014.This paper will give a brief introduction of the facility and the development status of pulsed magnetic fields research at WHMFC.In addition,it will describe the application development of pulsed magnetic fields in both scientific and industrial research.展开更多
This paper synthesized a new type of ternary piezoelectric catalyst MoO_(x)/ZnS/ZnO (MZZ)by a one-step method.The catalytic degradation of Rhodamine B (RhB) solution (10μg/g,pH=7.0) shows that the composite catalyst ...This paper synthesized a new type of ternary piezoelectric catalyst MoO_(x)/ZnS/ZnO (MZZ)by a one-step method.The catalytic degradation of Rhodamine B (RhB) solution (10μg/g,pH=7.0) shows that the composite catalyst has excellent piezoelectric catalytic activity under ultrasonic vibration (40 k Hz).The piezoelectric degradation rate of the optimal sample reached 0.054 min^(-1),which was about 2.5 times that of pure ZnO.X-ray diffraction(XRD),X-ray photoelectron spectroscopy (XPS),Raman,transmission electron microscopy(TEM),scanning electron microscopy (SEM),and electrochemical impedance spectroscopy(EIS) technologies were used to analyze the structure,morphology,and interface charge transfer properties of the MZZ piezocatalysts.The results showed that the composite catalyst may have a core-shell structure.ZnS is coated on the surface of ZnO,while MoO_(x)adheres to the surface of ZnS.This structure endowed MZZ larger specific surface area than ZnO,which benefits the RhB adsorption.More importantly,the formed heterojunction structure between ZnS and ZnO promotes the separation of positive and negative charges induced by the piezoelectric effect.MoO_(x)species may act as a charge trap to further promote more carriers to participate in the reaction.In addition,MoO_(x)may also be beneficial in adsorbing dyes.Active species capture experiments show that superoxide radicals and holes are the main active species in piezoelectric catalytic reactions on MZZ catalysts.展开更多
基金support from the Natural Science Foundation of Jiangsu Province(Grant No.BK20240036)the National Natural Science Foundation of China(Grant Nos.U24A20515,22276099,and 22361162668)Guangxi Key Research and Development Program,China(Grant No.Guike AB24010074)。
文摘We conducted a field campaign to investigate the chemical composition,sources,and light absorption of submicron aerosols(PM_(1))from early 2022 in Nanjing,China.The average concentration of PM_(1) was 31μg m^(−3),organics(33%)constituted the largest fraction,followed by nitrate(30%),sulfate(18%),ammonium(15%),chloride(3%),and rBC(2%).Four organic aerosol(OA)subcomponents were identified,including two primary OA(POA)and two secondary OA(SOA).The less-oxidized SOA(LO-OOA)contributes the most to the total OA mass(59%).LO-OOA is tightly correlated with the tracer ion C_(2)H_(4)O_(2)^(+)from levoglucosan,and another aged biomass-burning derived species,K_(3)SO_(4)^(+),suggesting it was likely influenced by aged biomass-burning OA.Our study also revealed that fireworks during the Spring Festival have a detrimental impact on air quality,contributing to secondary formation and accumulation under static winter meteorological conditions,prolonging the pollution duration.Also,LO-OOA was found to have the strongest light-absorbing ability.Our results highlight that the light absorption of LO-OOA can mainly be attributed to the C_(x)H_(y)N^(+) family,increased with the double-bond equivalent value.The more-oxidized SOA(MO-OOA)exhibited a negligible light absorption and was strongly correlated with daytime photochemical processes,implying a light-bleaching effect.This study enhances our understanding of the regional contribution of biomass combustion and fireworks to PM_(1) pollution in Nanjing,a typical megacity in the Yangtze River Delta region,during winter,aiding in the development of strategies for long-term air quality improvement in the region.
基金supported by the Natural Science Foundation of Hebei Province(Nos.D2019106042,D2020304038,and D2021106002)the National Natural Science Foundation of China(No.22276099)+1 种基金the State Environmental Protection Key Laboratory of Formation and Prevention of Urban Air Pollution Complex(No.2021080544)the Environmental Monitoring Research Foundation of Jiangsu Province(No.2211).
文摘VOCs(Volatile organic compounds)exert a vital role in ozone and secondary organic aerosol production,necessitating investigations into their concentration,chemical characteristics,and source apportionment for the effective implementation of measures aimed at preventing and controlling atmospheric pollution.FromJuly to October 2020,onlinemonitoringwas conducted in the main urban area of Shijiazhuang to collect data on VOCs and analyze their concentrations and reactivity.Additionally,the PMF(positive matrix factorization)method was utilized to identify the VOCs sources.Results indicated that the TVOCs(total VOCs)concentration was(96.7±63.4μg/m^3),with alkanes exhibiting the highest concentration of(36.1±26.4μg/m^3),followed by OVOCs(16.4±14.4μg/m^3).The key active components were alkenes and aromatics,among which xylene,propylene,toluene,propionaldehyde,acetaldehyde,ethylene,and styrene played crucial roles as reactive species.The sources derived from PMF analysis encompassed vehicle emissions,solvent and coating sources,combustion sources,industrial emissions sources,as well as plant sources,the contribution of which were 37.80%,27.93%,16.57%,15.24%,and 2.46%,respectively.Hence,reducing vehicular exhaust emissions and encouraging neighboring industries to adopt low-volatile organic solvents and coatings should be prioritized to mitigate VOCs levels.
文摘Ossification of the posterior longitudinal ligament(OPLL)is a degenerative disease characterized by progressive ectopic bone formation process,which can lead to severe neurological impairments and reduced quality of life.While the etiology of OPLL is generally considered multifactorial,there is no consensus regarding these contributing factors including genetic,endocrine,biomechanical,immune and lifestyle factors.Through accumulating evidence from multidisciplinary investigations,the pathophysiological connection between OPLL and endocrine-metabolic dysregulation is becoming increasingly clear.Nevertheless,comprehensive understanding of the relationship between the two is hindered by several problems,such as methodological limitations and inadequate mechanistic studies.This review takes a deep dive into the possible factors contributing to OPLL from all aspects of metabolism,including glucose metabolism,lipid metabolism,bone and mineral metabolism,leptin,vitamin,growth hormone/IGF-1 and sex hormones,highlighting their potential roles in the onset and progression of OPLL.Clarifying the etiology of OPLL and elucidating the underlying pathogenesis are crucial for advancing both early intervention strategies and therapeutic approaches in clinical management.Therefore,the endocrine and metabolic disorders in OPLL patients should become a focus of future research.
基金supported by Zhejiang Province Science Fund for Distinguished Young Scholars(No.LR24D050001)the Joint Funds of Zhejiang Provincial Natural Science Foundation of China(No.LZJMZ23D050002)+3 种基金the National Natural Science Foundation of China(No.42175116)the Scientific Research Foundation for Guilin University of Technology(No.GUTQDJJ2023046)supported by Guangxi Engineering Research Center of Comprehensive Treatment for Agricultural Non-Point Source Pollutionthe Modern Industry College of Ecology and Environmental Protection,Guilin University of Technology.
文摘PM_(2.5) and black carbon(BC)are important air pollutants impacting radiation balance,air quality,health,and ecosystems.Ozone(O_(3))levels are increasing despite decreases in other pollutants,posing a challenge for pollution control,especially in coastal cities like Zhoushan,where the monsoonal climate can exacerbate PM_(2.5) and ozone pollution.This study conducted continuous online measurements of major atmospheric pollutants in Zhoushan,Zhejiang Province,in 2020.The results indicate that the highest contribution from local air masses in Zhoushan is observed in spring,accounting for 17.7%,while the greatest average contribution from northern Zhejiang Province,Jiangsu Province,and Shanghai occurs in winter,at 18.5%.Pollutant concentrationswere seasonally variable,with PM_(2.5),BC,and sulfur dioxide concentrations 56.6%,36%,and 58.2%higher in the cold season compared to the warm season.The O_(3) in spring is approximately 50%higher than that in summer.Ship emissions significantly contributed to BC,nitrogen oxides(NO_(x)),and carbon monoxide in Zhoushan.In spring,PM_(2.5) sources included photochemical processes and northern air mass transport,while in winter,PM_(2.5) was due to regional transport.The inhibitory effect of PM_(2.5) on O_(3) formation in the Zhoushan area is relatively weak.Reducing NO_(x) emissions may increase O_(3),emphasizing the need for volatile organic compounds monitoring and regional control measures to improve air quality and ensure sustainable development in Zhoushan.
基金supported by grants from the Special Foundation of President of the Chinese Academy of Sciences(Grant No.,YZJJ2020QN27,YZJJ2021QN33)Anhui Provincial Natural Science Foundation(Grant No.,2108085MC79).
文摘HCLS1-associated protein X-1(HAX1)is a multifunctional mitochondrial protein involved in the regulation of apoptosis,a crucial process of programmed cell death,and mRNA processing.Despite its significance,limited structural data is available for HAX1,hindering a comprehensive understanding of its biological function.Notably,the caseinolytic mitochondrial matrix peptidase chaperone subunit B(CLPB)has been identified as an interacting partner of HAX1,yet the biophysical properties and binding affinity governing their interaction remain poorly defined.In this study,we present a thorough biophysical characterization of full-length human HAX1 and CLPB,accomplished through recombinant expression and purification.By employing size exclusion chromatography,dynamic light scattering,and circular dichroism spectroscopy,we successfully established their biophysical properties,revealing contrasting structural features,with CLPB displaying a-helical content and HAX1 exhibiting a disordered nature.Moreover,we employed solutionstate nuclear magnetic resonance(NMR)spectroscopy to probe their binding affinity.Our findings demonstrate the formation of stable multimeric complexes between HAX1 and CLPB,and we quantified a dissociation constant in the low range of micro-molar for their high affinity interaction.These results lay the foundation for further in-depth investigations into the dynamics and energetics governing the HAX1-CLPB interaction,ultimately contributing to a comprehensive understanding of their functional mechanisms.
基金Project supported by the National Key R&D Program of China(2021YFB3501204)the National Science Fund for Distinguished Young Scholars(51925605)+1 种基金the National Science Foundation for Excellent Young Scholars(52222107)the National Natural Science Foundation of China(52171195,52201036)。
文摘As one of the core components of a magnetic refrigerator,magnetic refrigeration materials are expected to have not only a considerable magnetocaloric effect but also excellent thermal conductivity.The poor thermal conductivity of many competitive oxide-based magnetic refrigerants,exemplified by EuTiO3-based compounds,acts as a major limitation to their practical application.Therefore,improving the thermal conductivity of magnetic refrigeration materials has become a research emphasis of magnetic refrigeration in recent years.In this work,a series of EuTiO_(3)(ETO)/Cu composites with different copper additives was prepared using a solid-phase reaction method by introducing appropriate amounts of copper powder.The influence of the introduction of copper on the phase composition,microstructure,thermal conductivity,and magnetocaloric effect of the composites was systematically investigated.Unexpectedly,the thermal conductivity of the composites is enhanced by up to 260%due to copper addition,accompanied by only a 5%decrease in magnetic entropy change and refrigerating capacity.Copper additive forms localized thermal conductive networks and promotes the densification process,resulting in significantly enhanced thermal conductivity of the composites.This work demonstrates the feasibility of improving the thermal conductivity of oxide-base d magnetic refrigeration materials by introducing highly thermally conductive substances.
基金financially supported by the National Natural Science Foundation of China(Nos.22209183,22225902,U22A20436)the Advanced Talents of Jiangsu University,China(No.23JDG027)。
文摘Developing efficient electrocatalysts for oxygen evolution reaction(OER)is imperative to enhance the overall efficiency of electrolysis systems and rechargeable metal-air batteries operating in aqueous solutions.High-entropy materials,featured with their distinctive multi-component properties,have found extensive application as catalysts in electrochemical energy storage and conversion devices.However,synthesizing nanostructured high-entropy compounds under mild conditions poses a significant challenge due to the difficulty in overcoming the immiscibility of multiple metallic constituents.In this context,the current study focuses on the synthesis of an array of nano-sized high entropy sulfides tailored for OER via a facile precursor pyrolysis method at low temperature.The representative compound,Fe Co Ni Cu Mn Sx,demonstrates remarkable OER performance,achieving a current density of 10 m A/cm^(2) at an overpotential of merely 220 m V and excellent stability with constant electrolysis at 100 m A/cm^(2) for over 400 h.The in-situ formed metal(oxy)hydroxide has been confirmed as the real active sites and its exceptional performance can be primarily attributed to the synergistic effects arising from its multiple components.Furthermore,the synthetic methodology presented here is versatile and can be extended to the preparation of high entropy phosphides,which also present favorable OER performance.This research not only introduces promising non-noble electrocatalysts for OER but also offers a facile approach to expand the family of nano high-entropy materials,contributing significantly to the field of electrochemical energy conversion.
基金Project supported by the National Key Research and Development Program of China(2021YFB3501204)the National Natural Science Foundation of China(52171054)+1 种基金the National Science Foundation for Distinguished Young Scholars(51925605)the National Science Foundation for Excellent Young Scholars(52222107)。
文摘Magnetic refrigeration(MR)technology is regarded as an ideal solution for cryogenic applications,relying on magnetocaloric materials which provide necessary chilling effect.A series of polycrystalline Tm_(1-x)Er_(x)Ni_(2)Si_(2)(x=0.2,0.4)compounds was synthesized,and their magnetic properties,magnetic phase transition together with magnetocaloric effect(MCE)were studied.The Tm_(1-x)Er_(x)Ni_(2)Si_(2)(x=0.2,0.4)compounds display a field-induced metamagnetic transition from antiferromagnetic(AFM)to ferromagnetism(FM)in excess of 0.2 T,respectively.Meanwhile,the AFM ground state is unstable.Under the field change of 0-2 T,the values of maximal magnetic entropy change(-ΔS_(M)^(max))and refrigerant capacity(RC)for Tm_(0.8)Er_(0.2)Ni_(2)Si_(2)compound are 17.9 J/(kg·K)and 83.5 J/kg,respectively.The large reversible MCE under low magnetic fields(≤2 T)indicates that Tm_(0.8)Er_(0.2)Ni_(2)Si_(2)compound can serve as potential candidate materials for cryogenic magnetic refrigeration.
基金supported by the National Natural Science Foundation of China(32471354 to T.C.and 82260400 to J.L)Natural Science Foundation of Hainan Province(No.822RC703 to J.L)。
文摘Discoidin domain receptors(DDRs)are single-pass transmembrane proteins belonging to receptor tyrosine kinases(RTKs)family,which are activated by collagen ligands with unusual slow,sustained kinetics,distinguishing them from canonical RTKs.While DDRs play critical roles in cell adhesion,differentiation,and cancer progression,their activation mechanisms remain partly understood.Here,we investigated the transmembrane domains(TMDs)of DDR1 and DDR2 to elucidate their interaction dynamics in membrane.Using bacterial adenylate cyclase two-hybrid(BACTH)assays,we demonstrated robust homotypic interactions and even stronger heterotypic associations between DDRTMDs.NMR spectroscopy of DDR1TMD and DDR2TMD reconstituted in lipid bilayer-mimetic bicelles showed obvious chemical shift alterations,further validating the stability of their heterocomplex formation.Systematic mutagenesis identified leucine zipper motifs rather than GXXXA motifs mediated both homo-and hetero-associations of DDR1TMD and DDR2TMD.These findings demonstrated the TMD as a critical mediator of DDRs oligomerization and revealed their interaction patterns within membrane.Our study advances the understanding of DDR signaling regulation and highlights transmembrane domain interactions as potential targets for modulating DDR-related pathologies.
基金funded by theGuangzhou Municipal Science and Tech-nology Bureau(No.2023A03J0507).
文摘Background:Lung cancer is the leading cause of cancer-related mortality,and while low-dose computed tomography screening may reduce mortality,emerging prognostic models show superior discriminative efficacy compared to age-and smoking history-based screening.However,further research is needed to assess their reliability in predicting lung cancer risk in high-risk patients.Methods:This study evaluated the predictive performance and quality of existing lung cancer prognostic models through a systematic review and meta-analysis.A comprehensive search was conducted in PubMed,Cochrane,Web of Science,CNKI,and Wanfang for articles published between January 1,2000,and February 13,2025,identifying population-basedmodels incorporating all available modeling data.Results:Among 72 analyzed studies,models were developed from Asian(28 studies,including 23 Chinese cohorts)and European/American(48 studies)populations,with only 6 focusing on nonsmokers.Twenty-one models included genetic markers,15 used clinical factors,and 40 integrated epidemiological predictors.Although 37 models underwent external validation,only 4 demonstrated minimal bias and clinical applicability.A meta-analysis of 11 repeatedly validated models revealed calibration and discrimination,though some lacked calibration data.Conclusions:Few lung cancer prognostic models exist for nonsmokers.Most models exhibit poor predictive performance in external validations,with significant bias and limited application scope.Widespread external validation,standardized model development,and reporting techniques are needed to accurately identify high-risk individuals and ensure applicability across diverse populations.
基金supported by the National Natural Science Foundation of China(51973166,52373097)Beijing Natural Science Foundation(2232064)。
文摘Pressure sensors are essential for a wide range of applica-tions,including health monitoring,industrial diagnostics,etc.However,achieving both high sensitivity and mechanical ability to withstand high pressure in a single material remains a significant challenge.This study introduces a high-performance cellulose hydrogel inspired by the biomi-metic layered porous structure of human skin.The hydrogel features a novel design composed of a soft layer with large macropores and a hard layer with small micropores,each of which contribute uniquely to its pressure-sensing capabilities.The macropores in the soft part facilitate significant deforma-tion and charge accumulation,providing exceptional sensitivity to low pressures.In contrast,the microporous structure in the hard part enhances pressure range,ensuring support under high pressures and preventing structural failure.The performance of hydrogel is further optimized through ion introduction,which improves its conductivity,and as well the sensitivity.The sensor demonstrated a high sensitivity of 1622kPa^(-1),a detec-tion range up to 160 kPa,excellent conductivity of 4.01 Sm^(-1),rapid response time of 33 ms,and a low detection limit of 1.6 Pa,outperforming most existing cellulose-based sensors.This innovative hierarchically porous architecture not only enhances the pressure-sensing performance but also offers a simple and effective approach for utilizing natural polymers in sensing technologies.The cellulose hydrogel demonstrates sig-nificant potential in both health monitoring and industrial applications,providing a sensitive,durable,and versatile solution for pressure sensing.
基金supported by the National Natural Science Foundation of China (No. 21177009)
文摘Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.
文摘An outburst of coal and gas is a major hazard in underground coal mining. It is generally accepted that an outburst occurs when certain conditions of stress, coal gassiness and physical-mechanical properties of coal are met. Outbursting is recognized as a two-step process, i.e., initiation and development. In this paper, we present a fully-coupled solid and fluid code to model the entire process of an outburst. The deformation, failure and fracture of solid （coal） are modeled with the discrete element method, and the flow of fluid （gas and water） such as free flow and Darcy flow are modeled with the lattice Boltzmann method. These two methods are coupled in a two-way process, i.e., the solid part provides a moving boundary condition and transfers momentum to the fluid, while the fluid exerts a dragging force upon the solid. Gas desorption from coal occurs at the solid-fluid boundary, and gas diffusion is implemented in the solid code where particles are assumed to be porous. A simple 2D example to simulate the process of an outburst with the model is also presented in this paper to demonstrate the capability of the coupled model.
基金supported by the National Natural Science Foundation of China(31640001 to C.X.,U21A20148 to X.Z.and C.X.)the Presidential Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(Y96XC11131,E26CCG27,and E26CCD15 to C.X.)。
文摘The ability to navigate long distances is essential for many animals to locate shelter,food,and breeding grounds.Magnetic sense has evolved in various migratory and homing species to orient them based on the geomagnetic field.A highly conserved ironsulfur cluster assembly protein IscA is proposed as an animal magnetoreceptor(MagR).Iron-sulfur cluster binding is also suggested to play an essential role in MagR magnetism and is thus critical in animal magnetoreception.In the current study,we provide evidence for distinct iron binding and iron-sulfur cluster binding in MagR in pigeons,an avian species that relies on the geomagnetic field for navigation and homing.Pigeon MagR showed significantly higher total iron content from both iron-and ironsulfur binding.Y65 in pigeon MagR was shown to directly mediate mononuclear iron binding,and its mutation abolished iron-binding capacity of the protein.Surprisingly,both iron binding and iron-sulfur binding demonstrated synergistic effects,and thus appear to be integral and indispensable to pigeon MagR magnetism.These results not only extend our current understanding of the origin and complexity of MagR magnetism,but also imply a possible molecular explanation for the huge diversity in animal magnetoreception.
基金We gratefully acknowledge the financial support of the National Key Research and Development Program of China(2016YFA0401700).
文摘Wuhan National High Magnetic Field Center(WHMFC)at Huazhong University of Science and Technology is one of the top-class research centers in the world,which can offer pulsed fields up to 90.6 T with different field waveforms for scientific research and has passed the final evaluation of the Chinese government in 2014.This paper will give a brief introduction of the facility and the development status of pulsed magnetic fields research at WHMFC.In addition,it will describe the application development of pulsed magnetic fields in both scientific and industrial research.
基金financially supported by National Natural Science Foundation of China (No. 22172144)Nature Science Foundation of Zhejiang Province (No. LY20B030004)。
文摘This paper synthesized a new type of ternary piezoelectric catalyst MoO_(x)/ZnS/ZnO (MZZ)by a one-step method.The catalytic degradation of Rhodamine B (RhB) solution (10μg/g,pH=7.0) shows that the composite catalyst has excellent piezoelectric catalytic activity under ultrasonic vibration (40 k Hz).The piezoelectric degradation rate of the optimal sample reached 0.054 min^(-1),which was about 2.5 times that of pure ZnO.X-ray diffraction(XRD),X-ray photoelectron spectroscopy (XPS),Raman,transmission electron microscopy(TEM),scanning electron microscopy (SEM),and electrochemical impedance spectroscopy(EIS) technologies were used to analyze the structure,morphology,and interface charge transfer properties of the MZZ piezocatalysts.The results showed that the composite catalyst may have a core-shell structure.ZnS is coated on the surface of ZnO,while MoO_(x)adheres to the surface of ZnS.This structure endowed MZZ larger specific surface area than ZnO,which benefits the RhB adsorption.More importantly,the formed heterojunction structure between ZnS and ZnO promotes the separation of positive and negative charges induced by the piezoelectric effect.MoO_(x)species may act as a charge trap to further promote more carriers to participate in the reaction.In addition,MoO_(x)may also be beneficial in adsorbing dyes.Active species capture experiments show that superoxide radicals and holes are the main active species in piezoelectric catalytic reactions on MZZ catalysts.
