AIM: To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS: The swab samples were collected from 8 patients with FK(Group 1 from th...AIM: To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS: The swab samples were collected from 8 patients with FK(Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes(Group 4 from the conjunctival sac). Bacterial 16 S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. RESULTS: Our metagenomic data showed that 97% of the sequence reads were categorized into 245 distinct bacterial genera, with 67.75±7.79 genera detected in Group 1, 73.80±13.44 in Group 2, 74.57±14.14 in Group 3, and 89.60±27.49 in Group 4. Compared with the healthy eyes(Group 4), both infected(Groups 1 and 2) and fellow eyes(Group 3) of the patients with FK showed reduced bacterial diversity and altered ocular surface microbiota compositions, with lower abundance of Corynebacterium and Staphylo coccus and higher abundances of Pseudomonas, Achromobacter, Caulobacter and Psychrobacter. CONCLUSION: Our report depicts the altered ocular surface bacterial community structures both in the affected and fellow eyes of patients with FK. These changes may contribute to the pathogenesis of FK or the increased risk for FK.展开更多
The hot deformation behavior of Pt−10Ir alloy was studied under a wide range of deformation parameters.At a low deformation temperature(950−1150℃),the softening mechanism is primarily dynamic recovery.In addition,dyn...The hot deformation behavior of Pt−10Ir alloy was studied under a wide range of deformation parameters.At a low deformation temperature(950−1150℃),the softening mechanism is primarily dynamic recovery.In addition,dynamic recrystallization by progressive lattice rotation near grain boundaries(DRX by LRGBs)and microshear bands assisted dynamic recrystallization(MSBs assisted DRX)coordinate the deformation.However,it is difficult for the dynamic softening to offset the stain hardening due to a limited amount of DRXed grains.At a high deformation temperature(1250−1350℃),three main DRX mechanisms associated with strain rates occur:DRX by LRGBs,DRX by a homogeneous increase in misorientation(HIM)and geometric DRX(GDRX).With increasing strain,DRX by LRGBs is enhanced gradually under high strain rates;the“pinch-off”effect is enhanced at low strain rates,which was conducive to the formation of a uniform and fine microstructure.展开更多
All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management...All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management for cooling or heating fails to self-regulate the temperature in dynamic seasonal temperature condition.Herein,inspired by the dual-temperature regulation function of the fur color changes on the backs and abdomens of penguins,a smart thermal management composite hydrogel(PNA@H-PM Gel)system was subtly created though an"on-demand"dual-layer structure design strategy.The PNA@H-PM Gel system features synchronous solar and thermal radiation modulation as well as tunable phase transition temperatures to meet the variable seasonal thermal requirements and energy-saving demands via self-adaptive radiative cooling and solar heating regulation.Furthermore,this system demonstrates superb modulations of both the solar reflectance(ΔR=0.74)and thermal emissivity(ΔE=0.52)in response to ambient temperature changes,highlighting efficient temperature regulation with average radiative cooling and solar heating effects of 9.6℃in summer and 6.1℃in winter,respectively.Moreover,compared to standard building baselines,the PNA@H-PM Gel presents a more substantial energy-saving cooling/heating potentials for energy-efficient buildings across various regions and climates.This novel solution,inspired by penguins in the real world,will offer a fresh approach for producing intelligent,energy-saving thermal management materials,and serve for temperature regulation under dynamic climate conditions and even throughout all seasons.展开更多
Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic mod...Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic modulus makes Elinvar alloys highly desirable in precision-control applications,including aerospace,electronics,and optical instruments.Currently,most of the used and studied Elinvar alloys are ferromagnetic alloys(FeNi and Fe-Pt)and antiferromagnetic alloys(Fe-Mn and γ-MnCu)[2–4].The Elinvar effect in these alloys typically originates from magnetostriction or magnetoelastic effects,which are magnetic fieldor magnetic transition-dependent[5].Consequently,these Elinvar alloys cannot function properly in the presence of a magnetic field owing to their Elinvar effect being closely tied to magnetic phase transition.Therefore,developing non-magneticdependent Elinvar alloys is highly essential to widen their practical applications.展开更多
Gifford-McMahon-type pulse-tube cryocoolers(GM-PTCs)working at liquid helium temperatures are promising in quantum technology and cryogenic physics for their high reliability and minimal vibration.These features stem ...Gifford-McMahon-type pulse-tube cryocoolers(GM-PTCs)working at liquid helium temperatures are promising in quantum technology and cryogenic physics for their high reliability and minimal vibration.These features stem from the fact that there are no extra moving parts introduced into the system.The rotary valve is a key component in GM-PTCs that transfers the output exergy from the compressor to the cold head.Because a low Carnot efficiency of 1.58%is achieved at liquid helium temperatures,optimizing the rotary valve is crucial for improving the efficiency of GM-PTCs.In this regard,an exergy-loss analysis method is proposed in this paper to quantitatively obtain the leakage loss and viscosity loss of a rotary valve by experimental measurements.The results show that viscosity loss accounts for more than 97.5%of the total exergy loss in the rotary valve,and that it is possible to improve the structure of the rotary valve by expanding the flow area by 1.5 times.To verify the method,the cooling temperature and power of a remote two-stage GM-PTC were monitored,with original or optimized rotary valves installed.The experimental results show that compared to the original rotary valve,the optimized rotary valve can improve the cooling efficiency of a GM-PTC by 16.4%,with a cooling power of 0.78 W at 4.2 K.展开更多
Although the degradability and biosafety of magnesium alloys make them advantageous for biological applications,medical implants made of magnesium alloys often fail prematurely due to corrosion.Therefore,improving the...Although the degradability and biosafety of magnesium alloys make them advantageous for biological applications,medical implants made of magnesium alloys often fail prematurely due to corrosion.Therefore,improving the corrosion resistance of magnesium alloys has become an urgent problem in the alloy design process.In this study,we designed and prepared Mg-xZn-0.5Y-0.5Zr(x=1,2,and 3,wt%)alloys in a hot extruded state and analyzed their surface structure through scanning electron microscopy,energy dispersion spectrometry,and X-ray diffraction.It was found that increasing the Zn content refined the recrystallized grains in the alloy.Particularly in Mg-3Zn-0.5Y-0.5Zr,the I phase became finer,forming both granular and nanoscale needle-like particles.Surface characterization after the immersion experiment showed that the corrosion product layer was mainly composed of Mg(OH)_(2),Zn(OH)_(2),CaCO_(3),and hy-droxyapatite.The degradation rate of ZW305K was the lowest,measured as 4.1 and 6.0 mm·a^(-1) with the hydrogen precipitation method and weight loss method respectively.Electrochemical experiments further explained the corrosion circuit model of the alloy in solution and confirmed the earlier results.The maximum polarization resistance of ZW305K was 874.5Ω·cm^(2),and the lowest corrosion current density was 0.104 mA·cm^(-2).As a biomedical alloy,it must exhibit good biocompatibility,so the alloy was also tested through cytotoxicity,cell adhesion,and staining experiments.The cell viability of each group after 48 h was greater than 80%,showing that the addition of zinc enhances the alloy’s biocompatibility.In summary,the prepared alloys have the potential to be used as biodegradable implant materials.展开更多
The adsorption/desorption of OH*on electrode surfaces is pivotal in numerous electrocatalytic reactions.To understand the effect of electrolyte pH on that process,in this work,an advanced approach combining ab initio ...The adsorption/desorption of OH*on electrode surfaces is pivotal in numerous electrocatalytic reactions.To understand the effect of electrolyte pH on that process,in this work,an advanced approach combining ab initio molecular dynamics(AIMD)with free energy perturbation is employed to calculate the dehydrogenation free energy of water chemisorbed at differently electrified Pt(111)/electrolyte interfaces.Our findings reveal that the onset potential for OH*formation shifts negatively as the pH increases at low pH condition(pH<4.3),aligning with the cyclic voltammetry curves observed in experimental studies.It indicates the dissociation of chemisorbed water is the primary route for OH*adsorption at low pH condition.Furthermore,it is also found that the variation in dehydrogenation energy across different pH is primarily due to the local hydrogen bonding network surrounding the chemisorbed water.In addition,it is proposed that at high pH conditions OH-oxidation emerges as the primary route for OH*adsorption on Pt(111)constrained by the water chemisorption process.This work provides crucial insights into the pH-dependent adsorption behavior of OH*on the Pt(111)surface and aims to guide the optimization of electrolytes to boost the efficiency of related reactions.展开更多
In order to promote the development of the characteristic industry of fragrant crisp peach in Honghuagang District of Zunyi City,Guizhou Province,and assist the implementation of the rural revitalization strategy,base...In order to promote the development of the characteristic industry of fragrant crisp peach in Honghuagang District of Zunyi City,Guizhou Province,and assist the implementation of the rural revitalization strategy,based on the actual situation of the local forest land,this paper studied the development of under-forest economy and the development of products with high added value.Through the combination of field investigation and laboratory analysis,the technology of interplanting soybean in peach orchard was discussed in depth,and the processing technology of high-quality preserved peach was developed.This paper is intended to expand and extend the industrial chain of fragrant and crisp peaches,and to promote the development of modern mountain characteristic and efficient agriculture.展开更多
The isotope effect on zonal flows(ZFs)and turbulence remains a key issue that is not completely solved in fusion plasmas.This paper presents the first experimental results of the ab initio prediction of causal relatio...The isotope effect on zonal flows(ZFs)and turbulence remains a key issue that is not completely solved in fusion plasmas.This paper presents the first experimental results of the ab initio prediction of causal relation between geodesic acoustic mode(GAM)and ambient turbulence at different isotope masses in the edge of HL-2A tokamak,where transfer entropy method based on information-theoretical approach is utilized as a quantified indicator of causality.Analysis shows that GAM is more pronounced in deuterium plasmas than in hydrogen,leading to a lower heat transport as well as more peaked profiles in the former situation.The causal impact of GAM on conductive heat flux component is stronger than on the convective component,which is resulted from a larger causal influence of zonal flow on temperature fluctuation.While a stronger GAM in deuterium plasmas has larger influence on all flux components,the relative change in temperature fluctuation and coefficient is more obvious when the ion mass varies.These findings not only offer an in-depth understanding of the real causality between zonal flow and turbulence in the present isotope experiments,but also provide useful ways for the physical understandings of transport and zonal flow dynamics in future deuterium-tritium fusion plasmas.展开更多
BACKGROUND There is no effective treatment for Alzheimer's disease(AD),and pharmacological treatment of AD in clinical settings is expensive and prolonged,resulting in a huge psychological and economic burden on t...BACKGROUND There is no effective treatment for Alzheimer's disease(AD),and pharmacological treatment of AD in clinical settings is expensive and prolonged,resulting in a huge psychological and economic burden on the patient's family and caregivers and society as a whole,AD is characterized by progressive,worsening cognitive impairment,and there are currently no drugs that can effectively reverse cognitive impairment.However,it is important to intervene early or delay cognitive impairment so that the condition can be delayed and,ultimately,the burden on patients and families can be reduced through maintenance treatment.It may be that non-pharmacological interventions such as cognitive stimulation therapy(CST)can help with cognitive dysfunction.AIM To provide a better treatment plan for AD patients and delay the deterioration of cognitive function,the effect of CST on cognitive function in AD was studied by Meta-analysis.METHODS Comprehensive search the Chinese and English databases were comprehensively searched by computer.Chinese databases:China Biomedical Literature Database(CBM),Wanfang Database,VIP Database,and China Periodicals Full-text Database(CNKI).The collection time limit is from July 21,2010 to July 21,2022 randomized controlled trials literature on the effects of CST on cognitive function in patients with AD.According to the inclusion and exclusion criteria,literature screening,data extraction,and quality evaluation were performed.Standardized mean difference(SMD)and 95%CI were used as evaluation criteria to evaluate the cognitive function of CST in AD patients.Sensitivity analysis and publication bias detection were performed on the results.Publication bias was assessed using funnel plots,and funnel plot symmetry was assessed with Eggr's test.RESULTS CST can not improve Mental State Examination Scale(MMSE)scores in AD patients.Meta-analysis of CST on MMSE scores showed that the heterogeneity was P=0.14,I2=35%.I2=35%<50%,and the Q test P>0.1,choose the random effect model to integrate statistics,get SMD=0.02,95%CI:-0.37,0.42,P>0.05.Meta-analysis of CST on AD Cognitive Functioning Assessment Scale scores showed that the heterogeneity was P=0.13,I2=36%.I2=36%<50 choose a fixed effect model to integrate statistics,get SMD=-0.01,95%CI:-0.40,0.39,P>0.05,the difference is not statistically significant.Meta-analysis of CST on the cognitive function indicators of patients showed that the heterogeneity was P=0.17,I2=31%.I2=31%<50%,the fixed effect model showed SMD=0.01,95%CI:-0.37,0.38,P>0.05,the difference was not statistically significant.CONCLUSION CST may not improve the cognitive function of AD patients,not improve the cognitive function of AD patients,not improve the ability of daily living,and not reduce mental behavior can improve the cognitive function of AD patients.展开更多
To effectively improve the adaptability and traversal abilities of a multi-terrain mobile robot under the dynamic excitation of multiple roads,we explore the mobile robot’s vibration and joint driving output stall ca...To effectively improve the adaptability and traversal abilities of a multi-terrain mobile robot under the dynamic excitation of multiple roads,we explore the mobile robot’s vibration and joint driving output stall caused by the dynamic excitation of the road spectrum function and analyze techniques for reducing the vibration and enhancing the driving moment of a four-wheel differential-speed mobile robot.A double-wishbone vibration reduction suspension and a moment compensator were designed for a multi-terrain mobile robot by theoretically describing its suspensionwheel-road dynamics.Also,the mobile robot’s road adaptability and traversal abilities were mathematically characterized under dynamic excitation.Co-simulation in ADAMS-MATLAB/Simulink is performed such as the harsh condition of in situ rotation and outdoor experimental schemes are implemented in which the experimental data are analyzed.The experimental result verifies the correctness of the theoretical analysis,as well as the effectiveness of the vibration reduction suspension and the moment compensator.The compatibility of the mobile robot’s driving mechanisms with road traversal abilities has been improved under various terrain conditions in complex field operation scenarios.展开更多
The high band gap of zinc oxide(ZnO)has significantly limited its potential application for organic contaminant removal in photocatalysis.In this study,ZnO/halloysites(HNTs)composites(ZnO/HNTs)were prepared using a hi...The high band gap of zinc oxide(ZnO)has significantly limited its potential application for organic contaminant removal in photocatalysis.In this study,ZnO/halloysites(HNTs)composites(ZnO/HNTs)were prepared using a high-temperature calcination method to enhance the removal of tetracycline hydrochloride(TCH).The experimental results demonstrated that the band gap of ZnO/HNTs decreased to 3.12 eV,compared to 3.21 eV for pure ZnO.The observed removal rate(k_(obs))of TCH in the ZnO/HNTs/vis system was 1.90×10^(-2) min^(-1),significantly higher than the rates in the HNTs/vis(1.25×10^(-3)min^(-1))and ZnO/vis(1.13×10^(-2) min^(-1))systems.Additionally,ZnO/HNTs exhibited strong resistance to coexisting natural organic and inorganic matter,maintaining high pollutant removal efficiency in natural water samples.The ZnO/HNTs/vis system also effectively removed other common organic pollutants,such as ciprofloxacin and methylene blue.Cycle tests indicated that the ZnO/HNTs/vis system retained 65.57%of its original efficiency,demonstrating good reusability and versatility.Scavenging and electron paramagnetic resonance experiments identified that h+was the primary species in the ZnO/HNTs/vis system,with other species playing auxiliary roles in TCH degradation.This study provides valuable insights into the design of novel ZnO-based photocatalysts for the degradation of organic pollutants in water.展开更多
Recently,high-entropy materials are attracting enormous attention in battery applications,encompassing both electrode materials and solid electrolytes,due to the pliability and diversification in material composition ...Recently,high-entropy materials are attracting enormous attention in battery applications,encompassing both electrode materials and solid electrolytes,due to the pliability and diversification in material composition and electronic structure.Theoretically,the rapid ion transport and the abundance of surface defects in high-entropy materials suggest a potential for enhancing the performance of composite solid-state electrolytes(CPEs).Herein,using a high-entropy oxide(HEO)filler to assess its potential contributions to CPEs is proposed.The distinctive structural distortions in HEO significantly improve the ionic conductivity(5×10^(−4) S·cm^(−1) at 60℃)and Li-ion transference number(0.57)of CPEs.Furthermore,the enhanced Li-ion transport capability extends the critical current density from 0.6 to 1.5 mA·cm^(−2) in Li/Li symmetric cells.In addition,all-solid-state batteries incorporating the HEO-modified CPEs exhibit superior rate performance and cycling stability.The work will enrich the application of HEOs in CPEs and provide fundamental understanding.展开更多
AIM: To investigate the molecular mechanism for regulation of cholesterol metabolism by hepatitis C virus(HCV) core protein in Hep G2 cells.METHODS: HCV genotype 1b core protein was cloned and expressed in Hep G2 cell...AIM: To investigate the molecular mechanism for regulation of cholesterol metabolism by hepatitis C virus(HCV) core protein in Hep G2 cells.METHODS: HCV genotype 1b core protein was cloned and expressed in Hep G2 cells. The cholesterol content was determined after transfection. The expression of sterol regulatory element binding protein 2(SREBP2) and the rate-limiting enzyme in cholesterol synthesis(HMGCR) was measured by quantitative real-time PCR and immunoblotting after transfection. The effects of core protein on the SREBP2 promoter and 3'-untranslated region were analyzed by luciferase assay. We used different target predictive algorithms, micro RNA(mi RNA) mimics/inhibitors, and site-directed mutation to identify a putative target of a particular mi RNA.RESULTS: HCV core protein expression in Hep G2 cells increased the total intracellular cholesterol level(4.05 ± 0.17 vs 6.47 ± 0.68, P = 0.001), and this increase corresponded to an increase in SREBP2 and HMGCR m RNA levels(P = 0.009 and 0.037, respectively) and protein expression. The molecular mechanism studyrevealed that the HCV core protein increased the expression of SREBP2 by enhancing its promoter activity(P = 0.004). In addition, mi R-185-5p expression was tightly regulated by the HCV core protein(P = 0.041). Moreover, overexpression of mi R-185-5p repressed the SREBP2 m RNA level(P = 0.022) and protein expression. In contrast, inhibition of mi R-185-5p caused upregulation of SREBP2 protein expression. mi R-185-5p was involved in the regulation of SREBP2 expression by HCV core protein. CONCLUSION: HCV core protein disturbs the cholesterol homeostasis in Hep G2 cells via the SREBP2 pathway; mi R-185-5p is involved in the regulation of SREBP2 by the core protein.展开更多
AIM: To examine the association between interferon(IFN) therapy and loss of hepatitis B surface antigen(HBs Ag) in inactive HBs Ag carriers. METHODS: This was a retrospective cohort study in inactive HBs Ag carriers, ...AIM: To examine the association between interferon(IFN) therapy and loss of hepatitis B surface antigen(HBs Ag) in inactive HBs Ag carriers. METHODS: This was a retrospective cohort study in inactive HBs Ag carriers, who were treatment-naive, with a serum HBs Ag level < 100 IU/m L and an undetectable hepatitis B virus(HBV) DNA level(< 100 IU/m L). All the 20 treated patients received subcutaneous PEG-IFN alfa-2a 180 μg/wk for 72 wk and were then followed for 24 wk. There were 40 untreated controls matched with 96 wk of observation. Serum HBs Ag, HBV DNA, and alanine aminotransferases were monitored every 3 mo in the treatment group and every 3-6 mo in the control group. RESULTS: Thirteen(65.0%) of 20 treated patients achieved HBs Ag loss, 12 of whom achieved HBs Ag seroconversion. Mean HBs Ag level in treated patients decreased to 6.69 ± 13.04 IU/m L after 24 wk of treatment from a baseline level of 26.22 ± 33.00 IU/m L. Serum HBV DNA level remained undetectable(< 100 IU/m L) in all treated patients during the study. HBs Ag level of the control group decreased from 25.72 ± 25.58 IU/m L at baseline to 17.11 ± 21.62 IU/m L at week 96(P = 0.108). In the control group, no patient experienced HBs Ag loss/seroconversion, and two(5.0%) developed HBV reactivation.CONCLUSION: IFN treatment results in HBs Ag loss and seroconversion in a considerable proportion of inactive HBs Ag carriers with low HBs Ag concentrations.展开更多
High ionic conductivity and superior interfacial stability of solid electrolytes at the electrodes are crucial factors for high-performance all-solid-state sodium batteries. Herein, a composite solid electrolyte Na3PS...High ionic conductivity and superior interfacial stability of solid electrolytes at the electrodes are crucial factors for high-performance all-solid-state sodium batteries. Herein, a composite solid electrolyte Na3PS4-polyethylene oxide is synthesized by the solution-phase reaction method with an improved ionic conductivity up to 9.4 × 10-5 S/cm at room temperature. Moreover, polyethylene oxide polymer layer is wrapped homogeneously on the surface of Na3PS4 particles, which could effectively avoid the direct contact between Na3PS4 electrolyte and sodium metal, thus alleviate their side reactions. We demonstrate that all-solid-state battery SnS2/Na with the composite solid electrolyte Na3PS4-polyethylene oxide delivers an enhanced electrochemical performance with 230 m Ah/g after 40 cycles.展开更多
Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and cancer.As the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of A...Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and cancer.As the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of An-Luo-Hua-Xian pill(ALHX)on fibrosis regression in CHB patients treated with entecavir(ETV).Methods:Treatment-naïve patients with CHB were randomly treated with ETV alone or combined with ALHX(ETV+ALHX)between October 1,2013 and December 31,2020.Demographic,laboratory,and liver histology data before and after 78 weeks of treatment were collected.The Ishak fibrosis score(F)was used and fibrosis regression required a decrease in F of≥1 after treatment.Results:A total of 780 patients were enrolled,and 394 with a second liver biopsy after treatment were included in the per-protocol population,132 in ETV group and 262 in ETV+ALHX group.After 78 weeks of treatment,the fibrosis regression rate in the ETV+ALHX group was significantly higher than that of the ETV group at baseline F≥3 patients:124/211(58.8%)vs.45/98(45.9%),p=0.035.The percentage of patients with a decreased liver stiffness measurement(LSM)was higher in the ETV+ALHX group:156/211(73.9%)vs.62/98(63.%),p=0.056.Logistic regression analysis showed that ETV combined with ALHX was associated with fibrosis regression[odds ratio(OR)=1.94,p=0.018],and a family history of hepatocellular carcinoma was on the contrary.(OR=0.41,p=0.031).Conclusions:ETV combined with ALHX increased liver fibrosis regression in CHB patients.展开更多
Background: Accumulating evidence demonstrates that microRNAs(miRNAs) play essential roles in tumorigenesis and cancer progression of hepatocellular carcinoma(HCC). Average targets of a miRNA were more than 100. And o...Background: Accumulating evidence demonstrates that microRNAs(miRNAs) play essential roles in tumorigenesis and cancer progression of hepatocellular carcinoma(HCC). Average targets of a miRNA were more than 100. And one miRNA may act in tumor via regulating several targets. The present study aimed to explore more potential targets of miR-449a by proteomics technology and further uncover the role of miR-449a in HCC tumorigenesis.Methods: Technologies such as i TRAQ-based quantitative proteomic were used to investigate the effect of miR-449a on HCC. The expression of c-Met and miR-449a was detected by q RT-PCR in HCC samples.Gain-and loss-of-function experiments were performed to identify the function and potential target of miR-449a in HCC cells.Results: In HCC, miR-449a was significantly downregulated, while c-Met was upregulated concurrently.Quantitative proteomics and luciferase reporter assay identified c-Met as a direct target of miR-449a.Moreover, miR-449a inhibited HCC growth not only by targeting CDK6 but also by suppressing cMet/Ras/Raf/ERK signaling pathway. Furthermore, the inhibition of c-Met expression with a specific siRNA significantly inhibited cells growth and deregulated the ERK pathway in HCC.Conclusion: The tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway.展开更多
基金Supported by the National Natural Science Foundation of China(No.81670839)the Key Research and Development Plan of Shandong Province(No.2018CXGC1205)
文摘AIM: To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS: The swab samples were collected from 8 patients with FK(Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes(Group 4 from the conjunctival sac). Bacterial 16 S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. RESULTS: Our metagenomic data showed that 97% of the sequence reads were categorized into 245 distinct bacterial genera, with 67.75±7.79 genera detected in Group 1, 73.80±13.44 in Group 2, 74.57±14.14 in Group 3, and 89.60±27.49 in Group 4. Compared with the healthy eyes(Group 4), both infected(Groups 1 and 2) and fellow eyes(Group 3) of the patients with FK showed reduced bacterial diversity and altered ocular surface microbiota compositions, with lower abundance of Corynebacterium and Staphylo coccus and higher abundances of Pseudomonas, Achromobacter, Caulobacter and Psychrobacter. CONCLUSION: Our report depicts the altered ocular surface bacterial community structures both in the affected and fellow eyes of patients with FK. These changes may contribute to the pathogenesis of FK or the increased risk for FK.
基金financial supports from the National Natural Science Foundation of China(Nos.52161023,51901204)Science and Technology Project of Yunnan Precious Metal Laboratory,China(No.YPML-2023050208)+1 种基金Yunnan Science and Technology Planning Project,China(Nos.202201AU070010,202301AT070276,202302AB080008,202303AA080001)Postgraduate Research and Innovation Foundation of Yunnan University,China(No.2021Y338).
文摘The hot deformation behavior of Pt−10Ir alloy was studied under a wide range of deformation parameters.At a low deformation temperature(950−1150℃),the softening mechanism is primarily dynamic recovery.In addition,dynamic recrystallization by progressive lattice rotation near grain boundaries(DRX by LRGBs)and microshear bands assisted dynamic recrystallization(MSBs assisted DRX)coordinate the deformation.However,it is difficult for the dynamic softening to offset the stain hardening due to a limited amount of DRXed grains.At a high deformation temperature(1250−1350℃),three main DRX mechanisms associated with strain rates occur:DRX by LRGBs,DRX by a homogeneous increase in misorientation(HIM)and geometric DRX(GDRX).With increasing strain,DRX by LRGBs is enhanced gradually under high strain rates;the“pinch-off”effect is enhanced at low strain rates,which was conducive to the formation of a uniform and fine microstructure.
基金the funding and generous support of the National Natural Science Foundation of China(52103263,52271249)the Key Project of International Science&Technology Cooperation of Shaanxi Province(2023-GHZD-09)+5 种基金the Key Project of Science Foundation of Education Department of Shaanxi Province(22JY011)the Key Project of Scientific Research and Development of Shaanxi Province(2023GXLH-070)the Qinchuangyuan"Scientist+Engineer"Team of Shaanxi Province(2023KXJ-069)the Key Research and Development Program of Shaanxi(2023-YBGY-488)the Sci-tech Innovation Team of Shaanxi Province(2024RS-CXTD-46)the Key Research and Development Program of Shaanxi Province(2020ZDLGY13-11).
文摘All-season thermal management with zero energy consumption and emissions is more crucial to global decarbonization over traditional energy-intensive cooling/heating systems.However,the static single thermal management for cooling or heating fails to self-regulate the temperature in dynamic seasonal temperature condition.Herein,inspired by the dual-temperature regulation function of the fur color changes on the backs and abdomens of penguins,a smart thermal management composite hydrogel(PNA@H-PM Gel)system was subtly created though an"on-demand"dual-layer structure design strategy.The PNA@H-PM Gel system features synchronous solar and thermal radiation modulation as well as tunable phase transition temperatures to meet the variable seasonal thermal requirements and energy-saving demands via self-adaptive radiative cooling and solar heating regulation.Furthermore,this system demonstrates superb modulations of both the solar reflectance(ΔR=0.74)and thermal emissivity(ΔE=0.52)in response to ambient temperature changes,highlighting efficient temperature regulation with average radiative cooling and solar heating effects of 9.6℃in summer and 6.1℃in winter,respectively.Moreover,compared to standard building baselines,the PNA@H-PM Gel presents a more substantial energy-saving cooling/heating potentials for energy-efficient buildings across various regions and climates.This novel solution,inspired by penguins in the real world,will offer a fresh approach for producing intelligent,energy-saving thermal management materials,and serve for temperature regulation under dynamic climate conditions and even throughout all seasons.
基金financially supported by the National Natural Science Foundation of China(No.52401161)the Natural Science Foundation of Sichuan Province for Young Scholars(No.24NSFSC6582)+3 种基金the Postdoctoral Fellowship Program of CPSF(No.GZC20231761)the National Natural Science Foundation of China(No.52271249)the Key Research and Development Program of Shaanxi(No.2023-YBGY-488)the Xi'an Talent Plan(No.XAYC240016).
文摘Elinvar alloys exhibit temperature-independent elastic modulus within a specific temperature range,known as the Elinvar effect,which was first observed in Fe-Ni alloys[1].The unique temperature-independent elastic modulus makes Elinvar alloys highly desirable in precision-control applications,including aerospace,electronics,and optical instruments.Currently,most of the used and studied Elinvar alloys are ferromagnetic alloys(FeNi and Fe-Pt)and antiferromagnetic alloys(Fe-Mn and γ-MnCu)[2–4].The Elinvar effect in these alloys typically originates from magnetostriction or magnetoelastic effects,which are magnetic fieldor magnetic transition-dependent[5].Consequently,these Elinvar alloys cannot function properly in the presence of a magnetic field owing to their Elinvar effect being closely tied to magnetic phase transition.Therefore,developing non-magneticdependent Elinvar alloys is highly essential to widen their practical applications.
基金supported by the National Key Research&Development Program of China(No.2023YFF0721304)the Key Research&Development Program of Jiangsu Province(No.2021015-4),China。
文摘Gifford-McMahon-type pulse-tube cryocoolers(GM-PTCs)working at liquid helium temperatures are promising in quantum technology and cryogenic physics for their high reliability and minimal vibration.These features stem from the fact that there are no extra moving parts introduced into the system.The rotary valve is a key component in GM-PTCs that transfers the output exergy from the compressor to the cold head.Because a low Carnot efficiency of 1.58%is achieved at liquid helium temperatures,optimizing the rotary valve is crucial for improving the efficiency of GM-PTCs.In this regard,an exergy-loss analysis method is proposed in this paper to quantitatively obtain the leakage loss and viscosity loss of a rotary valve by experimental measurements.The results show that viscosity loss accounts for more than 97.5%of the total exergy loss in the rotary valve,and that it is possible to improve the structure of the rotary valve by expanding the flow area by 1.5 times.To verify the method,the cooling temperature and power of a remote two-stage GM-PTC were monitored,with original or optimized rotary valves installed.The experimental results show that compared to the original rotary valve,the optimized rotary valve can improve the cooling efficiency of a GM-PTC by 16.4%,with a cooling power of 0.78 W at 4.2 K.
基金supported by the National Natural Science Foundation of China(Nos.52371070 and 52271249),the Key Research and Development Program of Shaanxi,China(No.2023-YBGY-488)the State Key Laboratory of Solidification Processing in Northwestern Polytechnical University(No.SKLSP202415)Additional support was provided by the Xi’an Talent Plan,China(No.XAYC240016)。
文摘Although the degradability and biosafety of magnesium alloys make them advantageous for biological applications,medical implants made of magnesium alloys often fail prematurely due to corrosion.Therefore,improving the corrosion resistance of magnesium alloys has become an urgent problem in the alloy design process.In this study,we designed and prepared Mg-xZn-0.5Y-0.5Zr(x=1,2,and 3,wt%)alloys in a hot extruded state and analyzed their surface structure through scanning electron microscopy,energy dispersion spectrometry,and X-ray diffraction.It was found that increasing the Zn content refined the recrystallized grains in the alloy.Particularly in Mg-3Zn-0.5Y-0.5Zr,the I phase became finer,forming both granular and nanoscale needle-like particles.Surface characterization after the immersion experiment showed that the corrosion product layer was mainly composed of Mg(OH)_(2),Zn(OH)_(2),CaCO_(3),and hy-droxyapatite.The degradation rate of ZW305K was the lowest,measured as 4.1 and 6.0 mm·a^(-1) with the hydrogen precipitation method and weight loss method respectively.Electrochemical experiments further explained the corrosion circuit model of the alloy in solution and confirmed the earlier results.The maximum polarization resistance of ZW305K was 874.5Ω·cm^(2),and the lowest corrosion current density was 0.104 mA·cm^(-2).As a biomedical alloy,it must exhibit good biocompatibility,so the alloy was also tested through cytotoxicity,cell adhesion,and staining experiments.The cell viability of each group after 48 h was greater than 80%,showing that the addition of zinc enhances the alloy’s biocompatibility.In summary,the prepared alloys have the potential to be used as biodegradable implant materials.
基金support from the National Natural Science Foundation of China(Nos.22272193,22225302,21991151,and 21991150)the Ningbo Natural Science Foundation(No.2022J294)+1 种基金the Yongjiang Talent Introduction Program(No.2021A-115-G)Key Laboratory of Advanced Marine Materials,Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo.(No.2021A-115-G).
文摘The adsorption/desorption of OH*on electrode surfaces is pivotal in numerous electrocatalytic reactions.To understand the effect of electrolyte pH on that process,in this work,an advanced approach combining ab initio molecular dynamics(AIMD)with free energy perturbation is employed to calculate the dehydrogenation free energy of water chemisorbed at differently electrified Pt(111)/electrolyte interfaces.Our findings reveal that the onset potential for OH*formation shifts negatively as the pH increases at low pH condition(pH<4.3),aligning with the cyclic voltammetry curves observed in experimental studies.It indicates the dissociation of chemisorbed water is the primary route for OH*adsorption at low pH condition.Furthermore,it is also found that the variation in dehydrogenation energy across different pH is primarily due to the local hydrogen bonding network surrounding the chemisorbed water.In addition,it is proposed that at high pH conditions OH-oxidation emerges as the primary route for OH*adsorption on Pt(111)constrained by the water chemisorption process.This work provides crucial insights into the pH-dependent adsorption behavior of OH*on the Pt(111)surface and aims to guide the optimization of electrolytes to boost the efficiency of related reactions.
基金Supported by Zunyi City Municipal and University Joint Science and Technology R&D Funding Project(HZ2023159)Zunyi Science and Technology Support Program Project(NS2023015)+2 种基金Guizhou Provincial Science and Technology Commissioner Innovation and Entrepreneurship Service Training Demonstration Base(HHG2023001)Honghuagang District Science and Technology Cooperation Project(HZ[2022]No.08)Honghuagang District Science and Technology Cooperation Project(HZ[2022]No.03).
文摘In order to promote the development of the characteristic industry of fragrant crisp peach in Honghuagang District of Zunyi City,Guizhou Province,and assist the implementation of the rural revitalization strategy,based on the actual situation of the local forest land,this paper studied the development of under-forest economy and the development of products with high added value.Through the combination of field investigation and laboratory analysis,the technology of interplanting soybean in peach orchard was discussed in depth,and the processing technology of high-quality preserved peach was developed.This paper is intended to expand and extend the industrial chain of fragrant and crisp peaches,and to promote the development of modern mountain characteristic and efficient agriculture.
基金supported by the National MCF Energy Research and Development Program(Grant Nos.2024YFE03190001,2024YFE03190004,2022YFE03030001,and 2019YFE03030002)the National Natural Science Foundation of China(Grant Nos.12405257,12475215,and 12475219)+2 种基金the Natural Science Foundation of Sichuan Province,China(Grant Nos.2023NSFSC1289 and 2025ZNSFSC0066)the Nuclear Technology Research and Development Program(Grant No.HJSYF2024(02))the Innovation Program of Southwestern Institute of Physics(Grant No.202301XWCX001)。
文摘The isotope effect on zonal flows(ZFs)and turbulence remains a key issue that is not completely solved in fusion plasmas.This paper presents the first experimental results of the ab initio prediction of causal relation between geodesic acoustic mode(GAM)and ambient turbulence at different isotope masses in the edge of HL-2A tokamak,where transfer entropy method based on information-theoretical approach is utilized as a quantified indicator of causality.Analysis shows that GAM is more pronounced in deuterium plasmas than in hydrogen,leading to a lower heat transport as well as more peaked profiles in the former situation.The causal impact of GAM on conductive heat flux component is stronger than on the convective component,which is resulted from a larger causal influence of zonal flow on temperature fluctuation.While a stronger GAM in deuterium plasmas has larger influence on all flux components,the relative change in temperature fluctuation and coefficient is more obvious when the ion mass varies.These findings not only offer an in-depth understanding of the real causality between zonal flow and turbulence in the present isotope experiments,but also provide useful ways for the physical understandings of transport and zonal flow dynamics in future deuterium-tritium fusion plasmas.
基金Supported by Liaoning Provincial Natural Science Foundation,No.2022-MS-430Shenyang Science and Technology Bureau Project,No.22-321-33-56Shenyang Municipal Health Commission Project,No.2022006.
文摘BACKGROUND There is no effective treatment for Alzheimer's disease(AD),and pharmacological treatment of AD in clinical settings is expensive and prolonged,resulting in a huge psychological and economic burden on the patient's family and caregivers and society as a whole,AD is characterized by progressive,worsening cognitive impairment,and there are currently no drugs that can effectively reverse cognitive impairment.However,it is important to intervene early or delay cognitive impairment so that the condition can be delayed and,ultimately,the burden on patients and families can be reduced through maintenance treatment.It may be that non-pharmacological interventions such as cognitive stimulation therapy(CST)can help with cognitive dysfunction.AIM To provide a better treatment plan for AD patients and delay the deterioration of cognitive function,the effect of CST on cognitive function in AD was studied by Meta-analysis.METHODS Comprehensive search the Chinese and English databases were comprehensively searched by computer.Chinese databases:China Biomedical Literature Database(CBM),Wanfang Database,VIP Database,and China Periodicals Full-text Database(CNKI).The collection time limit is from July 21,2010 to July 21,2022 randomized controlled trials literature on the effects of CST on cognitive function in patients with AD.According to the inclusion and exclusion criteria,literature screening,data extraction,and quality evaluation were performed.Standardized mean difference(SMD)and 95%CI were used as evaluation criteria to evaluate the cognitive function of CST in AD patients.Sensitivity analysis and publication bias detection were performed on the results.Publication bias was assessed using funnel plots,and funnel plot symmetry was assessed with Eggr's test.RESULTS CST can not improve Mental State Examination Scale(MMSE)scores in AD patients.Meta-analysis of CST on MMSE scores showed that the heterogeneity was P=0.14,I2=35%.I2=35%<50%,and the Q test P>0.1,choose the random effect model to integrate statistics,get SMD=0.02,95%CI:-0.37,0.42,P>0.05.Meta-analysis of CST on AD Cognitive Functioning Assessment Scale scores showed that the heterogeneity was P=0.13,I2=36%.I2=36%<50 choose a fixed effect model to integrate statistics,get SMD=-0.01,95%CI:-0.40,0.39,P>0.05,the difference is not statistically significant.Meta-analysis of CST on the cognitive function indicators of patients showed that the heterogeneity was P=0.17,I2=31%.I2=31%<50%,the fixed effect model showed SMD=0.01,95%CI:-0.37,0.38,P>0.05,the difference was not statistically significant.CONCLUSION CST may not improve the cognitive function of AD patients,not improve the cognitive function of AD patients,not improve the ability of daily living,and not reduce mental behavior can improve the cognitive function of AD patients.
基金Supported by Anhui Engineering Research Center on Information Fusion and Control of Intelligent Robot(Grant No.IFCIR2024014)Open Fund Key Laboratory of Machine Vision Inspection of Anhui Provincial,China(Grant No.KLMVI-2024-HIT-14)+2 种基金University Synergy Innovation Program of Anhui Province,China(Grant No.GXXT-2023-076)Anhui Future Technology Research Institute Enterprise Cooperation Project(Grant No.2023qyhz35)2024 Wuhu Science and Technology Planning Project(Grant Nos.2024cj40,2024cxy24).
文摘To effectively improve the adaptability and traversal abilities of a multi-terrain mobile robot under the dynamic excitation of multiple roads,we explore the mobile robot’s vibration and joint driving output stall caused by the dynamic excitation of the road spectrum function and analyze techniques for reducing the vibration and enhancing the driving moment of a four-wheel differential-speed mobile robot.A double-wishbone vibration reduction suspension and a moment compensator were designed for a multi-terrain mobile robot by theoretically describing its suspensionwheel-road dynamics.Also,the mobile robot’s road adaptability and traversal abilities were mathematically characterized under dynamic excitation.Co-simulation in ADAMS-MATLAB/Simulink is performed such as the harsh condition of in situ rotation and outdoor experimental schemes are implemented in which the experimental data are analyzed.The experimental result verifies the correctness of the theoretical analysis,as well as the effectiveness of the vibration reduction suspension and the moment compensator.The compatibility of the mobile robot’s driving mechanisms with road traversal abilities has been improved under various terrain conditions in complex field operation scenarios.
基金financially supported by the National Natural Science Foundation of China(Nos.52270149,51908528,2200013)Natural Science Foundation of Henan Province,China(No.242300421443)+1 种基金The Science and Technology Key Project of Henan Province,China(No.242102321073)Doctoral Fund Project of Henan University of Technology,China(Nos.2020BS005,2023BS004).
文摘The high band gap of zinc oxide(ZnO)has significantly limited its potential application for organic contaminant removal in photocatalysis.In this study,ZnO/halloysites(HNTs)composites(ZnO/HNTs)were prepared using a high-temperature calcination method to enhance the removal of tetracycline hydrochloride(TCH).The experimental results demonstrated that the band gap of ZnO/HNTs decreased to 3.12 eV,compared to 3.21 eV for pure ZnO.The observed removal rate(k_(obs))of TCH in the ZnO/HNTs/vis system was 1.90×10^(-2) min^(-1),significantly higher than the rates in the HNTs/vis(1.25×10^(-3)min^(-1))and ZnO/vis(1.13×10^(-2) min^(-1))systems.Additionally,ZnO/HNTs exhibited strong resistance to coexisting natural organic and inorganic matter,maintaining high pollutant removal efficiency in natural water samples.The ZnO/HNTs/vis system also effectively removed other common organic pollutants,such as ciprofloxacin and methylene blue.Cycle tests indicated that the ZnO/HNTs/vis system retained 65.57%of its original efficiency,demonstrating good reusability and versatility.Scavenging and electron paramagnetic resonance experiments identified that h+was the primary species in the ZnO/HNTs/vis system,with other species playing auxiliary roles in TCH degradation.This study provides valuable insights into the design of novel ZnO-based photocatalysts for the degradation of organic pollutants in water.
基金supported by the National Natural Science Foundation of China(No.52002094)Shenzhen Science and Technology Program(Nos.JCYJ20210324121411031,JSGG202108021253804014 and RCBS20210706092218040)Shenzhen Steady Support Plan(Nos.GXWD20221030205923001 and GXWD20201230155427003-20200824103000001).
文摘Recently,high-entropy materials are attracting enormous attention in battery applications,encompassing both electrode materials and solid electrolytes,due to the pliability and diversification in material composition and electronic structure.Theoretically,the rapid ion transport and the abundance of surface defects in high-entropy materials suggest a potential for enhancing the performance of composite solid-state electrolytes(CPEs).Herein,using a high-entropy oxide(HEO)filler to assess its potential contributions to CPEs is proposed.The distinctive structural distortions in HEO significantly improve the ionic conductivity(5×10^(−4) S·cm^(−1) at 60℃)and Li-ion transference number(0.57)of CPEs.Furthermore,the enhanced Li-ion transport capability extends the critical current density from 0.6 to 1.5 mA·cm^(−2) in Li/Li symmetric cells.In addition,all-solid-state batteries incorporating the HEO-modified CPEs exhibit superior rate performance and cycling stability.The work will enrich the application of HEOs in CPEs and provide fundamental understanding.
基金Supported by Medical Specialty Development Projects of Beijing Municipal Administration of Hospitals,No.ZYLX201402Ministry of Education of The People’s Republic of China,No.20121107110012+1 种基金Beijing Municipal Commission of Education,No.11320016Collaborative Innovation Center of Infectious Diseases and Beijing Key Laboratory of Emerging Infectious Diseases,Beijing,China
文摘AIM: To investigate the molecular mechanism for regulation of cholesterol metabolism by hepatitis C virus(HCV) core protein in Hep G2 cells.METHODS: HCV genotype 1b core protein was cloned and expressed in Hep G2 cells. The cholesterol content was determined after transfection. The expression of sterol regulatory element binding protein 2(SREBP2) and the rate-limiting enzyme in cholesterol synthesis(HMGCR) was measured by quantitative real-time PCR and immunoblotting after transfection. The effects of core protein on the SREBP2 promoter and 3'-untranslated region were analyzed by luciferase assay. We used different target predictive algorithms, micro RNA(mi RNA) mimics/inhibitors, and site-directed mutation to identify a putative target of a particular mi RNA.RESULTS: HCV core protein expression in Hep G2 cells increased the total intracellular cholesterol level(4.05 ± 0.17 vs 6.47 ± 0.68, P = 0.001), and this increase corresponded to an increase in SREBP2 and HMGCR m RNA levels(P = 0.009 and 0.037, respectively) and protein expression. The molecular mechanism studyrevealed that the HCV core protein increased the expression of SREBP2 by enhancing its promoter activity(P = 0.004). In addition, mi R-185-5p expression was tightly regulated by the HCV core protein(P = 0.041). Moreover, overexpression of mi R-185-5p repressed the SREBP2 m RNA level(P = 0.022) and protein expression. In contrast, inhibition of mi R-185-5p caused upregulation of SREBP2 protein expression. mi R-185-5p was involved in the regulation of SREBP2 expression by HCV core protein. CONCLUSION: HCV core protein disturbs the cholesterol homeostasis in Hep G2 cells via the SREBP2 pathway; mi R-185-5p is involved in the regulation of SREBP2 by the core protein.
文摘AIM: To examine the association between interferon(IFN) therapy and loss of hepatitis B surface antigen(HBs Ag) in inactive HBs Ag carriers. METHODS: This was a retrospective cohort study in inactive HBs Ag carriers, who were treatment-naive, with a serum HBs Ag level < 100 IU/m L and an undetectable hepatitis B virus(HBV) DNA level(< 100 IU/m L). All the 20 treated patients received subcutaneous PEG-IFN alfa-2a 180 μg/wk for 72 wk and were then followed for 24 wk. There were 40 untreated controls matched with 96 wk of observation. Serum HBs Ag, HBV DNA, and alanine aminotransferases were monitored every 3 mo in the treatment group and every 3-6 mo in the control group. RESULTS: Thirteen(65.0%) of 20 treated patients achieved HBs Ag loss, 12 of whom achieved HBs Ag seroconversion. Mean HBs Ag level in treated patients decreased to 6.69 ± 13.04 IU/m L after 24 wk of treatment from a baseline level of 26.22 ± 33.00 IU/m L. Serum HBV DNA level remained undetectable(< 100 IU/m L) in all treated patients during the study. HBs Ag level of the control group decreased from 25.72 ± 25.58 IU/m L at baseline to 17.11 ± 21.62 IU/m L at week 96(P = 0.108). In the control group, no patient experienced HBs Ag loss/seroconversion, and two(5.0%) developed HBV reactivation.CONCLUSION: IFN treatment results in HBs Ag loss and seroconversion in a considerable proportion of inactive HBs Ag carriers with low HBs Ag concentrations.
基金funding support from 1000 Talent Plan program(NO.31370086963030)research projects from Shandong Province(2018JMRH0211,2017CXGC1010 and 2016GGX104001)+2 种基金Taishan Scholar Program(11370085961006)the National Science Foundation of Shandong Province(ZR2017MEM002)the Fundamental Research Funds of Shandong University(201810422046,2017JC010,2017JC042,and 2016JC005)。
文摘High ionic conductivity and superior interfacial stability of solid electrolytes at the electrodes are crucial factors for high-performance all-solid-state sodium batteries. Herein, a composite solid electrolyte Na3PS4-polyethylene oxide is synthesized by the solution-phase reaction method with an improved ionic conductivity up to 9.4 × 10-5 S/cm at room temperature. Moreover, polyethylene oxide polymer layer is wrapped homogeneously on the surface of Na3PS4 particles, which could effectively avoid the direct contact between Na3PS4 electrolyte and sodium metal, thus alleviate their side reactions. We demonstrate that all-solid-state battery SnS2/Na with the composite solid electrolyte Na3PS4-polyethylene oxide delivers an enhanced electrochemical performance with 230 m Ah/g after 40 cycles.
基金supported by National Science and Technology Major Project(2013ZX10002005 and 2017ZX10203202).
文摘Background and Aims:Chronic hepatitis B(CHB)can cause liver fibrosis and lead to cirrhosis and cancer.As the effectiveness of antiviral therapy to reverse liver fibrosis is limited,We aimed to evaluate the effect of An-Luo-Hua-Xian pill(ALHX)on fibrosis regression in CHB patients treated with entecavir(ETV).Methods:Treatment-naïve patients with CHB were randomly treated with ETV alone or combined with ALHX(ETV+ALHX)between October 1,2013 and December 31,2020.Demographic,laboratory,and liver histology data before and after 78 weeks of treatment were collected.The Ishak fibrosis score(F)was used and fibrosis regression required a decrease in F of≥1 after treatment.Results:A total of 780 patients were enrolled,and 394 with a second liver biopsy after treatment were included in the per-protocol population,132 in ETV group and 262 in ETV+ALHX group.After 78 weeks of treatment,the fibrosis regression rate in the ETV+ALHX group was significantly higher than that of the ETV group at baseline F≥3 patients:124/211(58.8%)vs.45/98(45.9%),p=0.035.The percentage of patients with a decreased liver stiffness measurement(LSM)was higher in the ETV+ALHX group:156/211(73.9%)vs.62/98(63.%),p=0.056.Logistic regression analysis showed that ETV combined with ALHX was associated with fibrosis regression[odds ratio(OR)=1.94,p=0.018],and a family history of hepatocellular carcinoma was on the contrary.(OR=0.41,p=0.031).Conclusions:ETV combined with ALHX increased liver fibrosis regression in CHB patients.
基金supported by grants from Shenzhen Municipal Science and Technology Foundation(JCYJ20160425103340738)the Natural Science Foundation of Zhejiang Province(LY15H160021)
文摘Background: Accumulating evidence demonstrates that microRNAs(miRNAs) play essential roles in tumorigenesis and cancer progression of hepatocellular carcinoma(HCC). Average targets of a miRNA were more than 100. And one miRNA may act in tumor via regulating several targets. The present study aimed to explore more potential targets of miR-449a by proteomics technology and further uncover the role of miR-449a in HCC tumorigenesis.Methods: Technologies such as i TRAQ-based quantitative proteomic were used to investigate the effect of miR-449a on HCC. The expression of c-Met and miR-449a was detected by q RT-PCR in HCC samples.Gain-and loss-of-function experiments were performed to identify the function and potential target of miR-449a in HCC cells.Results: In HCC, miR-449a was significantly downregulated, while c-Met was upregulated concurrently.Quantitative proteomics and luciferase reporter assay identified c-Met as a direct target of miR-449a.Moreover, miR-449a inhibited HCC growth not only by targeting CDK6 but also by suppressing cMet/Ras/Raf/ERK signaling pathway. Furthermore, the inhibition of c-Met expression with a specific siRNA significantly inhibited cells growth and deregulated the ERK pathway in HCC.Conclusion: The tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway.
