Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered specie...Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.展开更多
基金supported by National Natural Science Foundation of China(Award Number:2346842).
文摘Cryopreservation of living cells and tissues plays a vital role in biomedical research,clinical applications,biotechnology innovation,the development of new vaccines and drugs,and the conservation of endangered species.While significant technological breakthroughs have been achieved in cooling methods-particularly through vitrification for large tissue and organs-the lack of optimal rewarming technology remains a key obstacle to successful cryopreservation,especially for larger samples such as tissues and organs.The primary challenges during the warming process include non-uniformity heating and insufficient rewarming rates,which can lead to thermal stress-induced structural damage and lethal ice recrystallization,ultimately compromising the integrity and functionality of biological materials.In recent years,various advanced warming techniques have emerged,employing different energy conversion approaches to achieve volumetric heating while minimizing the risk of overheating.These techniques involve thermal,mechanical-thermal,and electromagnetic-thermal energy conversions.However,each method presents its own limitation.This review aims to summarize recent advancements in rewarming technologies for cryopreservation,with a focus on their mechanisms,applications,and the key challenges that must be addressed to enable broader adoption in medical and commercial contexts.
文摘为了改善人参和覆盆子复合培养基制备香料香气品格特性,该研究采用从清香型大曲中分离得到的产香异常威克汉姆酵母(Wickerhamomyces anomalus)SMR-WA08发酵人参-覆盆子复合培养基。通过单因素试验探讨初始pH、发酵温度、转速、接种量、种龄、装液量和发酵时间对致香成分相对含量的影响,并结合响应面法优化菌株SMR-WA08产香条件,运用非靶向代谢组学技术分析其香气成分与差异代谢物。结果表明,最佳产香条件为:初始pH 5.5、发酵温度26℃、转速110 r/min、接种量5%、种龄30 h、装液量100 m L/250 m L、发酵时间4 d。在此优化条件下,致香成分相对含量为(184.55±2.84)%。代谢组学分析结果显示,实验组(T组)与空白对照组(C组)之间存在显著代谢差异,基于变量重要性投影(VIP)值>1且P<0.05共筛选出798个显著差异代谢物,其中333个上调,465个下调。该研究结果显示,异常威克汉姆酵母发酵能有效调控人参-覆盆子基质中相关代谢通路及代谢物,从而丰富和改善产香品质。
