Metastasis remains the primary cause of cancer-related mortality worldwide.Circulating tumor cells(CTCs)represent critical targets for metastasis prevention and treatment.Traditional Chinese medicine may prevent lung ...Metastasis remains the primary cause of cancer-related mortality worldwide.Circulating tumor cells(CTCs)represent critical targets for metastasis prevention and treatment.Traditional Chinese medicine may prevent lung cancer metastasis through long-term intervention in CTC activity.Tiao-Shen-Zhi-Ai Formular(TSZAF)represents a Chinese medicine compound prescription utilized clinically for lung cancer treatment.This study combined three principal active ingredients from TSZAF into a novel TSZAF monomer combination(TSZAF mc)to investigate its anti-metastatic effects and mechanisms.TSZAF mc demonstrated significant inhibition of proliferation,migration,and invasion in CTC-TJH-01 and LLC cells,while inducing cellular apoptosis in vitro.Moreover,TSZAF mc substantially inhibited LLC cell growth and metastasis in vivo.Mechanistically,TAZSF mc significantly suppressed the Wnt/β-catenin signaling pathway and CXCL5 expression in lung cancer cells and tissues.Additionally,TAZSF mc notably reduced neutrophil infiltration in metastatic lesions.These findings indicate that TSZAF mc inhibits lung cancer growth and metastasis by suppressing the Wnt/β-catenin signaling pathway and reducing CXCL5 secretion,thereby decreasing neutrophil recruitment and infiltration.TSZAF mc demonstrates potential as an effective therapeutic agent for lung cancer metastasis.展开更多
基金sponsored by the National Natural Science Foundation of China(Nos.81973517,82174245,82174017,and 82305069)Shanghai Shenkang Hospital Development Center’s Second Round of"Three-Year Action Plan to Promote Clinical Skills and Clinical Innovation in Municipal Hospitals"Research Physician Innovation and Translational Ability Training Project(No.SHDC2023CRD01)+2 种基金Shanghai Institute of Traditional Chinese Medicine and Psychosomatic Diseases 2023 Open Research Project(No.SZB2023102)Science and Technology Development Program of Shanghai University of Traditional Chinese Medicine in 2023(No.23KFL096)Traditional Chinese Medicine Science and Technology Development Project of Shanghai Medical Innovation&Development Foundation(No.WL-HBRC-2021001K)。
文摘Metastasis remains the primary cause of cancer-related mortality worldwide.Circulating tumor cells(CTCs)represent critical targets for metastasis prevention and treatment.Traditional Chinese medicine may prevent lung cancer metastasis through long-term intervention in CTC activity.Tiao-Shen-Zhi-Ai Formular(TSZAF)represents a Chinese medicine compound prescription utilized clinically for lung cancer treatment.This study combined three principal active ingredients from TSZAF into a novel TSZAF monomer combination(TSZAF mc)to investigate its anti-metastatic effects and mechanisms.TSZAF mc demonstrated significant inhibition of proliferation,migration,and invasion in CTC-TJH-01 and LLC cells,while inducing cellular apoptosis in vitro.Moreover,TSZAF mc substantially inhibited LLC cell growth and metastasis in vivo.Mechanistically,TAZSF mc significantly suppressed the Wnt/β-catenin signaling pathway and CXCL5 expression in lung cancer cells and tissues.Additionally,TAZSF mc notably reduced neutrophil infiltration in metastatic lesions.These findings indicate that TSZAF mc inhibits lung cancer growth and metastasis by suppressing the Wnt/β-catenin signaling pathway and reducing CXCL5 secretion,thereby decreasing neutrophil recruitment and infiltration.TSZAF mc demonstrates potential as an effective therapeutic agent for lung cancer metastasis.
