CO_(2)electrolysis using solid oxide electrolysis cells is a promising technology for CO_(2)utilization and conversion,which has attracted more and more attention in recent years because of its extremely high efficien...CO_(2)electrolysis using solid oxide electrolysis cells is a promising technology for CO_(2)utilization and conversion,which has attracted more and more attention in recent years because of its extremely high efficiency.However,traditional Ni-yttria-stabilized zirconia(Ni-YSZ)or Ni-Gd_(0.1)Ce_(0.9)O_(2-δ)(Ni-GDC)metal-ceramic cathode faces many problems such as Ni agglomeration and carbon deposition during long-time operation.Herein,a perovskite oxide La_(0.43-x)Ca_(0.37)Ti_(0.9)Ni_(0.1)O_(3-δ)(LCTN,x=0,0.05,0.1)with nanophase-LaVO_(4)exsolution was investigated as the novel cathode of solid oxide electrolysis cell(SOEC)for efficient CO_(2)electrolysis.The results confirm that the exsolution nanophase on LCTN surface can significantly improve the CO_(2)adsorption and conversion performance.For CO_(2)electrolysis at 1.8 V,an electrolysis current density of 1.24 A/cm2at 800℃can be obtained on SOEC with La_(0.43-x)Ca_(0.37)Ti_(0.9)Ni_(0.1)O_(3-δ)decorated with LaVO_(4)(LCTN-V0.05)cathode.Furthermore,the corresponding cell can maintain stable operation up to 100 h without apparent performance degradation.These results demonstrate that doping-induced second nanophase exsolution is a promising way to design high-performance SOEC cathodes for CO_(2)electrolysis.展开更多
The machining precision of blades is critical to the service performance of aero engines.The Leading Edge(LE) of high-pressure compressor blades poses a challenge for precision machining due to its thin size, high deg...The machining precision of blades is critical to the service performance of aero engines.The Leading Edge(LE) of high-pressure compressor blades poses a challenge for precision machining due to its thin size, high degree of bending, and significant change of curvature. Aimed at optimizing the machining error, this paper presents a framework that integrates toolpath planning and process parameter regulation. Firstly, an Iterative Subdivision Algorithm(ISA) for clamped Bspline curve is proposed, based on which toolpath planning method towards the LE is developed.Secondly, the removal effect of Cutter Contact(CC) point on the sampling points is investigated in the calculation of grinding dwell time by traversing in u-v space. A global material removal model is constructed for the solution. Thirdly, the previous two steps are interconnected based on the Improved Whale Optimization Algorithm(IWOA), and the optimal parameter combination is searched using the Root Mean Square Error(RMSE) of the machining error as the objective function. Based on this, the off-line programming and robotic grinding experiments are carried out. The experimental results show that the proposed method with error optimization can achieve 0.0143 mm mean value and 0.0160 mm standard deviations of LE surface error, which is an improvement of32.5% and 33.9%, respectively, compared with previous method.展开更多
Polygonati rhizoma is often used in Chinese medicine and as food.In this study,atmospheric pressure matrixassisted laser desorption ionization and quadruple-time-of-flight(MALDI-Q-TOF)mass spectrometry techniques were...Polygonati rhizoma is often used in Chinese medicine and as food.In this study,atmospheric pressure matrixassisted laser desorption ionization and quadruple-time-of-flight(MALDI-Q-TOF)mass spectrometry techniques were applied to P.rhizoma samples from Polygonatum cyrtonema Hua species.Positive ions were mainly detected in the mass range of m/z 200-600,while negative ions were mainly observed in the mass range of m/z 100-450.A total of 263 components were identified and the spatial distribution and changes in saccharides contents during the steaming process of P.rhizoma were investigated.Monosaccharide and disaccharide exhibit a relatively uniform distribution,while the oligosaccharides were mainly found in the bast of fresh P.rhizoma.Although the contents of monosaccharide and disaccharide were increased during steaming,that of trisaccharide,tetrasaccharide,and pentasaccharide were decreased.We used the 5 saccharide types with the greatest variation in content as variables for the principal component analysis(PCA)and cluster analysis.Both PCA and cluster analysis showed that these 5 saccharides can be used as markers in the steaming process of the P.rhizoma.Present study of mass spectrometry imaging provides novel insights into the spatiotemporal accumulation patterns of saccharides in P.rhizoma,improving our understanding of the steaming process.展开更多
Although supported solid amine adsorbents have attracted great attention for CO_(2) capture,critical chemical deactivation problems including oxidative degradation and urea formation have severely restricted their pra...Although supported solid amine adsorbents have attracted great attention for CO_(2) capture,critical chemical deactivation problems including oxidative degradation and urea formation have severely restricted their practical applications for flue gas CO_(2) capture.In this work,we reveal that the nature of surface hydroxyl groups(metal hydroxyl Al–OH and nonmetal hydroxyl Si–OH)plays a key role in the deactivation mechanisms.The polyethyleneimine(PEI)supported on Al–OH-containing substrates suffers from severe oxidative degradation during the CO_(2) capture step due to the breakage of amine-support hydrogen bonding networks,but exhibits an excellent anti-urea formation feature by preventing dehydration of carbamate products under a pure CO_(2) regeneration atmosphere.In contrast,PEI supported on Si–OHcontaining substrates exhibits excellent anti-oxidative stability under simulated flue gas conditions by forming a robust hydrogen bonding protective network with Si–OH,but suffers from obvious urea formation during the pure CO_(2) regeneration step.We also reveal that the urea formation problem for PEI-SBA-15 can be avoided by the incorporation of an OH-containing PEG additive.Based on the intrinsic understanding of degradation mechanisms,we successfully synthesized an adsorbent 40PEI-20PEG-SBA-15 that demonstrates outstanding stability and retention of a high CO_(2) capacity of 2.45 mmol g^(−1) over 1000 adsorption–desorption cycles,together with negligible capacity loss during aging in simulated flue gas(10%CO_(2)+5%O_(2)+3%H_(2)O)for one month at 60–70℃.We believe this work makes great contribution to the advancement in the field of ultra-stable solid amine-based CO_(2) capture materials.展开更多
As an application instructional course for Aquatic Animal Medicine(AAM),Aquatic Animal Pathogen Biology needs to be guided by a large number of examples and cases,but the current case database construction faces many ...As an application instructional course for Aquatic Animal Medicine(AAM),Aquatic Animal Pathogen Biology needs to be guided by a large number of examples and cases,but the current case database construction faces many urgent problems.In view of this,this paper analyzes the characteristics of professional education of AAM postgraduate students.With the goal of cultivating applied personnel that meet the requirements of the times,and the guiding ideology of strengthening the reform of the education model in colleges and universities,and improving the quantity and quality of personnel training,it builds a case database for the course Aquatic Animal Pathogen Biology in accordance with modern postgraduate teaching needs.展开更多
Chemotherapy may cause cellular oxidative stress to bone marrow.Oxidative damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment.Angelica s...Chemotherapy may cause cellular oxidative stress to bone marrow.Oxidative damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment.Angelica sinensis polysaccharides(ASP)are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress features.In the current study,we investigated the protective roles and mechanisms of ASP on chemotherapy-induced bone marrow stromal cell(BMSC)damage.The human bone marrow stromal cell line HS-5 cells were divided into control group,5-FU group,5-FU+ASP group,and 5-FU+LiCl group to investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation inhibition.The results showed that 5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner;however,ASP partially counteracted the 5-FU-induced decrease in cell viability,whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on HS-5 cells.ASP reversed the decrease in total cytoplasmicβ-catenin,p-GSK-3β,and CyclinD1 following 5-FU treatment and modulated nuclear expression ofβ-catenin,Lef-1,and C-myc proteins.Furthermore,ASP also enhanced the antioxidant capacity of cells and reduced 5-FU-induced oxidative stress,attenuated FoxO1 expression,thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27^(Kip1) expression to alleviate 5-FU-induced apoptosis and to promote cell cycle progression.All the results above suggest that the protective role of ASP in 5-FU-treated BMSCs proliferation for the chemotherapy may be related to its activating Wnt/β-catenin signaling and keeping homeostasis betweenβ-catenin and FoxO1 under oxidative stress.The study provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.展开更多
In order to improve the professional competitiveness of students in the Aquatic Animal Medicine Discipline,combined with the case tracking and theoretical practice for aquatic animal medicine students for three years,...In order to improve the professional competitiveness of students in the Aquatic Animal Medicine Discipline,combined with the case tracking and theoretical practice for aquatic animal medicine students for three years,with the aid of SPSS software,this paper elaborated the actual level and existing problems in career planning of aquatic animal medicine students in Guangdong Ocean University from five dimensions:career planning awareness,self-awareness,environmental awareness,goal planning,and implementation correction. Then,it discussed the opportunities and challenges of the Aquatic Animal Medicine Discipline in the context of the Belt and Road Initiative. Finally,it came up with solutions and reform recommendations in education,teaching,and counseling paths from the students,university,families,government,and society.展开更多
As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduate...As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.展开更多
This paper intends to combine the development trend of the subject,restructure and optimize the course content,and construct the service learning model of Pathogenic Biology of Aquatic Animals for postgraduates.At the...This paper intends to combine the development trend of the subject,restructure and optimize the course content,and construct the service learning model of Pathogenic Biology of Aquatic Animals for postgraduates.At the same time,through service practice,it is needed to further consolidate curriculum knowledge and skills,stimulate the learning initiative and enthusiasm of postgraduates,expand professional knowledge,improve professional quality,and lay a solid foundation for serving the national rural revitalization strategy in the future.展开更多
Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fiel...Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fields today.The Belt and Road Initiative has brought opportunities and challenges to the upgrading and development of this discipline,so this discipline faces many problems that need to be solved.In view of this,this study combined the development trend of the discipline,reorganized and optimized the course content,and prepared sophisticated multimedia courseware,to stimulate students'learning initiative and enthusiasm,expand the field of professional knowledge,improve their English translation and writing skills,so as to lay a solid foundation for their future service to the Belt and Road Initiative.展开更多
The giant muscle protein titin known for its mechanical stability serves as an ideal model for developing protein-based biomaterials.However,practical applications are hindered by challenges such as low expression yie...The giant muscle protein titin known for its mechanical stability serves as an ideal model for developing protein-based biomaterials.However,practical applications are hindered by challenges such as low expression yields,poor solubility,and limited thermal stability.In this study,we combined artificial intelligence(AI)tools—ProteinMPNN and Alpha-Fold2—with human insights and steered molecular dynamics(SMD)simulations to redesign the titin Ig domain.This approach generated thousands of novel sequences,preserving structural features essential for mechanical stability.Six de novo proteins were experimentally validated,all demonstrating mechanical and kinetic stability comparable to the natural I27 domain at the 100 pN level.Notably,these proteins exhibited significantly improved physical properties,with up to a fivefold increase in expression yield and enhanced solubility.Circular dichroism and atomic force microscopy-based single molecule force spectroscopy(AFM-SMFS)confirmed proper folding and mechanical stability,while thermal stability was retained or improved in some designs.Control experiments with unguided random designs,which yielded negative results,underscored the critical role of integrating AI tools with domain-specific knowledge for functional outcomes.Our findings highlight the transformative potential of integrating AI-driven design with human expertise and computational simulations,enabling the development of mechanically robust proteins for applications in bioengineering and biomaterials science.展开更多
The authors explored the thermal decomposition characteristics of perfluoroisobutyronitrile–carbon dioxide(C_(4)F_(7)N–CO_(2))gas mixture as eco-friendly dielectric medium.The main by-products and decomposition mech...The authors explored the thermal decomposition characteristics of perfluoroisobutyronitrile–carbon dioxide(C_(4)F_(7)N–CO_(2))gas mixture as eco-friendly dielectric medium.The main by-products and decomposition mechanism of C_(4)F_(7)N–CO_(2)gas mixture under different temperature and gas pressure conditions were revealed and analysed.It was found that the thermal decomposition of 6%C_(4)F_(7)N–94%CO_(2)gas mixture starts at about 350°C(0.15 MPa),producing C_(3)F_(6) and CO first.Some other characteristic by-products such as CF_(4),C_(2)F_(6),CF_(3)CN,COF_(2) and(CN)_(2) could also be detected at higher temperature.The yield of C_(3)F_(6),(CN)_(2) increased with the temperature(lower than 450℃)first and then decreased when it reached to 500℃.While the yield of CO,C3F8,COF_(2) and CF_(3)CN increased with temperature(350–550℃).The generation of CF_(4) and C_(2)F_(6) begins at temperatures higher than 500℃,which can be used as the feature component of severe overheating fault.The thermal decomposition amount and by-products yield of C_(4)F_(7)N–CO_(2)gas mixture slowed down with the increase of gas pressure,indicating that C_(4)F_(7)N–CO_(2)gas mixture is quite suitable used at high-pressure equipment,especially high-voltage devices such as gas insulated switchgear.展开更多
基金Project supported by the National Key Research&Development Project(2023YFB4006001)National Natural Science Foundation of China(52172199)。
文摘CO_(2)electrolysis using solid oxide electrolysis cells is a promising technology for CO_(2)utilization and conversion,which has attracted more and more attention in recent years because of its extremely high efficiency.However,traditional Ni-yttria-stabilized zirconia(Ni-YSZ)or Ni-Gd_(0.1)Ce_(0.9)O_(2-δ)(Ni-GDC)metal-ceramic cathode faces many problems such as Ni agglomeration and carbon deposition during long-time operation.Herein,a perovskite oxide La_(0.43-x)Ca_(0.37)Ti_(0.9)Ni_(0.1)O_(3-δ)(LCTN,x=0,0.05,0.1)with nanophase-LaVO_(4)exsolution was investigated as the novel cathode of solid oxide electrolysis cell(SOEC)for efficient CO_(2)electrolysis.The results confirm that the exsolution nanophase on LCTN surface can significantly improve the CO_(2)adsorption and conversion performance.For CO_(2)electrolysis at 1.8 V,an electrolysis current density of 1.24 A/cm2at 800℃can be obtained on SOEC with La_(0.43-x)Ca_(0.37)Ti_(0.9)Ni_(0.1)O_(3-δ)decorated with LaVO_(4)(LCTN-V0.05)cathode.Furthermore,the corresponding cell can maintain stable operation up to 100 h without apparent performance degradation.These results demonstrate that doping-induced second nanophase exsolution is a promising way to design high-performance SOEC cathodes for CO_(2)electrolysis.
基金supported by the National Natural Science Foundation of China (No. 52075059)Graduate Scientific Research and Innovation Foundation of Chongqing (No. CYB23021)the Innovation Fund of Aero Engine Corporation of China (No. ZZCX-2022-019)。
文摘The machining precision of blades is critical to the service performance of aero engines.The Leading Edge(LE) of high-pressure compressor blades poses a challenge for precision machining due to its thin size, high degree of bending, and significant change of curvature. Aimed at optimizing the machining error, this paper presents a framework that integrates toolpath planning and process parameter regulation. Firstly, an Iterative Subdivision Algorithm(ISA) for clamped Bspline curve is proposed, based on which toolpath planning method towards the LE is developed.Secondly, the removal effect of Cutter Contact(CC) point on the sampling points is investigated in the calculation of grinding dwell time by traversing in u-v space. A global material removal model is constructed for the solution. Thirdly, the previous two steps are interconnected based on the Improved Whale Optimization Algorithm(IWOA), and the optimal parameter combination is searched using the Root Mean Square Error(RMSE) of the machining error as the objective function. Based on this, the off-line programming and robotic grinding experiments are carried out. The experimental results show that the proposed method with error optimization can achieve 0.0143 mm mean value and 0.0160 mm standard deviations of LE surface error, which is an improvement of32.5% and 33.9%, respectively, compared with previous method.
基金funded by the Science and Technology Innovation Program of Hunan Province(2022RC1224,2022ZYC010)the Changsha Science and Technology Program(kh2004018)the Training Program for Excellent Young Innovators of Changsha(kq2206064)。
文摘Polygonati rhizoma is often used in Chinese medicine and as food.In this study,atmospheric pressure matrixassisted laser desorption ionization and quadruple-time-of-flight(MALDI-Q-TOF)mass spectrometry techniques were applied to P.rhizoma samples from Polygonatum cyrtonema Hua species.Positive ions were mainly detected in the mass range of m/z 200-600,while negative ions were mainly observed in the mass range of m/z 100-450.A total of 263 components were identified and the spatial distribution and changes in saccharides contents during the steaming process of P.rhizoma were investigated.Monosaccharide and disaccharide exhibit a relatively uniform distribution,while the oligosaccharides were mainly found in the bast of fresh P.rhizoma.Although the contents of monosaccharide and disaccharide were increased during steaming,that of trisaccharide,tetrasaccharide,and pentasaccharide were decreased.We used the 5 saccharide types with the greatest variation in content as variables for the principal component analysis(PCA)and cluster analysis.Both PCA and cluster analysis showed that these 5 saccharides can be used as markers in the steaming process of the P.rhizoma.Present study of mass spectrometry imaging provides novel insights into the spatiotemporal accumulation patterns of saccharides in P.rhizoma,improving our understanding of the steaming process.
基金supported by the Fundamental Research Funds for the National Natural Science Foundation of China 52225003,22208021,22109004the National Key R&D Program of China 2022YFB4101702.
文摘Although supported solid amine adsorbents have attracted great attention for CO_(2) capture,critical chemical deactivation problems including oxidative degradation and urea formation have severely restricted their practical applications for flue gas CO_(2) capture.In this work,we reveal that the nature of surface hydroxyl groups(metal hydroxyl Al–OH and nonmetal hydroxyl Si–OH)plays a key role in the deactivation mechanisms.The polyethyleneimine(PEI)supported on Al–OH-containing substrates suffers from severe oxidative degradation during the CO_(2) capture step due to the breakage of amine-support hydrogen bonding networks,but exhibits an excellent anti-urea formation feature by preventing dehydration of carbamate products under a pure CO_(2) regeneration atmosphere.In contrast,PEI supported on Si–OHcontaining substrates exhibits excellent anti-oxidative stability under simulated flue gas conditions by forming a robust hydrogen bonding protective network with Si–OH,but suffers from obvious urea formation during the pure CO_(2) regeneration step.We also reveal that the urea formation problem for PEI-SBA-15 can be avoided by the incorporation of an OH-containing PEG additive.Based on the intrinsic understanding of degradation mechanisms,we successfully synthesized an adsorbent 40PEI-20PEG-SBA-15 that demonstrates outstanding stability and retention of a high CO_(2) capacity of 2.45 mmol g^(−1) over 1000 adsorption–desorption cycles,together with negligible capacity loss during aging in simulated flue gas(10%CO_(2)+5%O_(2)+3%H_(2)O)for one month at 60–70℃.We believe this work makes great contribution to the advancement in the field of ultra-stable solid amine-based CO_(2) capture materials.
基金Supported by Project of Degree and Postgraduate Education and Teaching Reform of Guangdong Ocean University(202020&202120)Project on Education and Teaching Reform of Higher Education Institutions in Guangdong Province+1 种基金Applied Personnel Training Course of Guangdong Ocean UniversityIndustry-University-Research Institute Cooperation Collaborative Education Teaching Content and Curriculum System Reform Project of Ministry of Education(202101134003).
文摘As an application instructional course for Aquatic Animal Medicine(AAM),Aquatic Animal Pathogen Biology needs to be guided by a large number of examples and cases,but the current case database construction faces many urgent problems.In view of this,this paper analyzes the characteristics of professional education of AAM postgraduate students.With the goal of cultivating applied personnel that meet the requirements of the times,and the guiding ideology of strengthening the reform of the education model in colleges and universities,and improving the quantity and quality of personnel training,it builds a case database for the course Aquatic Animal Pathogen Biology in accordance with modern postgraduate teaching needs.
基金supported by the National Natural Science Foundation of China(Grant No.81873103)the Foundation and Frontier Research Project of Chongqing Science and Technology Commission(Grant No.cstc2014jcyjA10001).
文摘Chemotherapy may cause cellular oxidative stress to bone marrow.Oxidative damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment.Angelica sinensis polysaccharides(ASP)are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress features.In the current study,we investigated the protective roles and mechanisms of ASP on chemotherapy-induced bone marrow stromal cell(BMSC)damage.The human bone marrow stromal cell line HS-5 cells were divided into control group,5-FU group,5-FU+ASP group,and 5-FU+LiCl group to investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation inhibition.The results showed that 5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner;however,ASP partially counteracted the 5-FU-induced decrease in cell viability,whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on HS-5 cells.ASP reversed the decrease in total cytoplasmicβ-catenin,p-GSK-3β,and CyclinD1 following 5-FU treatment and modulated nuclear expression ofβ-catenin,Lef-1,and C-myc proteins.Furthermore,ASP also enhanced the antioxidant capacity of cells and reduced 5-FU-induced oxidative stress,attenuated FoxO1 expression,thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27^(Kip1) expression to alleviate 5-FU-induced apoptosis and to promote cell cycle progression.All the results above suggest that the protective role of ASP in 5-FU-treated BMSCs proliferation for the chemotherapy may be related to its activating Wnt/β-catenin signaling and keeping homeostasis betweenβ-catenin and FoxO1 under oxidative stress.The study provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.
基金Supported by Project on Education and Teaching Reform of Higher Education Institutions in Guangdong ProvinceProject on Education and Teaching Reform of Guangdong Ocean University(XJG201806)Academic and Graduate Education Reform Project of Guangdong Ocean University(201709)
文摘In order to improve the professional competitiveness of students in the Aquatic Animal Medicine Discipline,combined with the case tracking and theoretical practice for aquatic animal medicine students for three years,with the aid of SPSS software,this paper elaborated the actual level and existing problems in career planning of aquatic animal medicine students in Guangdong Ocean University from five dimensions:career planning awareness,self-awareness,environmental awareness,goal planning,and implementation correction. Then,it discussed the opportunities and challenges of the Aquatic Animal Medicine Discipline in the context of the Belt and Road Initiative. Finally,it came up with solutions and reform recommendations in education,teaching,and counseling paths from the students,university,families,government,and society.
基金Supported by Guangdong Higher Education Teaching Reform ProjectGuangdong Ocean University Education and Teaching Reform Project(XJG201806)Guangdong Ocean University Degree and Postgraduate Education Reform Research Project(201709)
文摘As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.
基金Supported by the Research Project of Degree and Postgraduate Education Reform of Guangdong Ocean University(202120)Innovation Program of Postgraduate Education in Guangdong Province(Ejiaoyanhan[2022]No.1)。
文摘This paper intends to combine the development trend of the subject,restructure and optimize the course content,and construct the service learning model of Pathogenic Biology of Aquatic Animals for postgraduates.At the same time,through service practice,it is needed to further consolidate curriculum knowledge and skills,stimulate the learning initiative and enthusiasm of postgraduates,expand professional knowledge,improve professional quality,and lay a solid foundation for serving the national rural revitalization strategy in the future.
基金Supported by Project on Education and Teaching Reform of Higher Education Institutions in Guangdong ProvinceApplied Personnel Training Course of Guangdong Ocean University+1 种基金Industry-University-Research Institute Cooperation Collaborative Education Teaching Content and Curriculum System Reform Project of Ministry of Education(202101134003)Project of Degree and Postgraduate Education and Teaching Reform of Guangdong Ocean University(202020&202120).
文摘Aquatic Animal Bacteriology is a basic course of Aquatic Animal Medicine discipline.Bacteriology is not only a frontier subject in the field of life sciences,but also one of the most rapidly developing scientific fields today.The Belt and Road Initiative has brought opportunities and challenges to the upgrading and development of this discipline,so this discipline faces many problems that need to be solved.In view of this,this study combined the development trend of the discipline,reorganized and optimized the course content,and prepared sophisticated multimedia courseware,to stimulate students'learning initiative and enthusiasm,expand the field of professional knowledge,improve their English translation and writing skills,so as to lay a solid foundation for their future service to the Belt and Road Initiative.
基金the funding support from the National Natural Science Foundation of China(grant nos.22222703 and 22477058)the Natural Science Foundation of Jiangsu Province,China(grant no.BK20202004)the Fundamental Research Funds for the Central Universities,China(grant no.020514380335).
文摘The giant muscle protein titin known for its mechanical stability serves as an ideal model for developing protein-based biomaterials.However,practical applications are hindered by challenges such as low expression yields,poor solubility,and limited thermal stability.In this study,we combined artificial intelligence(AI)tools—ProteinMPNN and Alpha-Fold2—with human insights and steered molecular dynamics(SMD)simulations to redesign the titin Ig domain.This approach generated thousands of novel sequences,preserving structural features essential for mechanical stability.Six de novo proteins were experimentally validated,all demonstrating mechanical and kinetic stability comparable to the natural I27 domain at the 100 pN level.Notably,these proteins exhibited significantly improved physical properties,with up to a fivefold increase in expression yield and enhanced solubility.Circular dichroism and atomic force microscopy-based single molecule force spectroscopy(AFM-SMFS)confirmed proper folding and mechanical stability,while thermal stability was retained or improved in some designs.Control experiments with unguided random designs,which yielded negative results,underscored the critical role of integrating AI tools with domain-specific knowledge for functional outcomes.Our findings highlight the transformative potential of integrating AI-driven design with human expertise and computational simulations,enabling the development of mechanically robust proteins for applications in bioengineering and biomaterials science.
基金supported by the National Natural Science Foundation of China(Nos.51707137 and 51877157)the Science and Technology Projects of State Grid Co.,Ltd.(No.5200-201919063A-0-0-00).
文摘The authors explored the thermal decomposition characteristics of perfluoroisobutyronitrile–carbon dioxide(C_(4)F_(7)N–CO_(2))gas mixture as eco-friendly dielectric medium.The main by-products and decomposition mechanism of C_(4)F_(7)N–CO_(2)gas mixture under different temperature and gas pressure conditions were revealed and analysed.It was found that the thermal decomposition of 6%C_(4)F_(7)N–94%CO_(2)gas mixture starts at about 350°C(0.15 MPa),producing C_(3)F_(6) and CO first.Some other characteristic by-products such as CF_(4),C_(2)F_(6),CF_(3)CN,COF_(2) and(CN)_(2) could also be detected at higher temperature.The yield of C_(3)F_(6),(CN)_(2) increased with the temperature(lower than 450℃)first and then decreased when it reached to 500℃.While the yield of CO,C3F8,COF_(2) and CF_(3)CN increased with temperature(350–550℃).The generation of CF_(4) and C_(2)F_(6) begins at temperatures higher than 500℃,which can be used as the feature component of severe overheating fault.The thermal decomposition amount and by-products yield of C_(4)F_(7)N–CO_(2)gas mixture slowed down with the increase of gas pressure,indicating that C_(4)F_(7)N–CO_(2)gas mixture is quite suitable used at high-pressure equipment,especially high-voltage devices such as gas insulated switchgear.
