Intense pulsed light(IPL)is a novel non-thermal technology with characteristics of wide spectral range,high instantaneous energy and strong penetration,resulting in great antibacterial efficiency.It is currently widel...Intense pulsed light(IPL)is a novel non-thermal technology with characteristics of wide spectral range,high instantaneous energy and strong penetration,resulting in great antibacterial efficiency.It is currently widely used in the fields of canned food packaging,fruit and vegetable juices,and dairy products.To improve the ef-ficiency of IPL and explore the wavelength-related antibacterial mechanism,this paper analyzed antibacterial efficacy of different spectral composition of IPL wavelength bands on Escherichia coli(E.coli),as well as related photochemical and physical effects.Firstly,it was confirmed that the UV-C(Ultraviolet-C,100-280 nm)and UV-B(Ultraviolet-B,280-320 nm)wavelength bands were the main effective spectral compositions according to spectral filtering experiments.Both of these wavelength ranges exhibited photochemical effects,which promoted the significant generation of reactive oxygen species(ROS)within the cells.It can disrupt metabolic functions and alter the physical and chemical properties of the cell membranes.Moreover,when the UV-A(Ultraviolet-A,320-400 nm)and UV-B wavelength bands were removed,the appearance of the cells still showed signs of shrinkage and damage.The structure of the lipopolysaccharide layer on the bacterial outer membrane was disrupted,increasing the permeability of the outer membrane,which was attributed to the physical effect of IPL.In addition,after treating E.coli with IPL using only the infrared band,no obvious antibacterial efficacy was observed.The results of this study could provide direct guidance for industrial applications in the field of food safety,especially in the screening and optimization of specific wavelengths.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD2101005)“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2023C01173)the National Science Foundation of China(U24A20475).
文摘Intense pulsed light(IPL)is a novel non-thermal technology with characteristics of wide spectral range,high instantaneous energy and strong penetration,resulting in great antibacterial efficiency.It is currently widely used in the fields of canned food packaging,fruit and vegetable juices,and dairy products.To improve the ef-ficiency of IPL and explore the wavelength-related antibacterial mechanism,this paper analyzed antibacterial efficacy of different spectral composition of IPL wavelength bands on Escherichia coli(E.coli),as well as related photochemical and physical effects.Firstly,it was confirmed that the UV-C(Ultraviolet-C,100-280 nm)and UV-B(Ultraviolet-B,280-320 nm)wavelength bands were the main effective spectral compositions according to spectral filtering experiments.Both of these wavelength ranges exhibited photochemical effects,which promoted the significant generation of reactive oxygen species(ROS)within the cells.It can disrupt metabolic functions and alter the physical and chemical properties of the cell membranes.Moreover,when the UV-A(Ultraviolet-A,320-400 nm)and UV-B wavelength bands were removed,the appearance of the cells still showed signs of shrinkage and damage.The structure of the lipopolysaccharide layer on the bacterial outer membrane was disrupted,increasing the permeability of the outer membrane,which was attributed to the physical effect of IPL.In addition,after treating E.coli with IPL using only the infrared band,no obvious antibacterial efficacy was observed.The results of this study could provide direct guidance for industrial applications in the field of food safety,especially in the screening and optimization of specific wavelengths.
