A full—lerlgth cDNA(L／pCe／／)encoding an endo-1，4-β-glucanase(EGase)was isolated from pollen tubes of Lilium longiflorum Thunb．by RT-PCR and RACE．The deduced protein，which iS predicted to be a compact globular...A full—lerlgth cDNA(L／pCe／／)encoding an endo-1，4-β-glucanase(EGase)was isolated from pollen tubes of Lilium longiflorum Thunb．by RT-PCR and RACE．The deduced protein，which iS predicted to be a compact globular protein，is of 490 amino acids．in cluding a putative 21 amino acid-signal peptide．LlpCell shares high level of homology(about50％)with plant secreted EGases，which bear apparently neither trans-membrane domain．nor cellulose—binding domain(CBD)．LIpCell belongs to gycosyl hydrolase family 9．LlpOell transcripts were detected in pollen grains．germinating pollen and elongating pollen tubes with a similar level．whereas LlpCell transcripts were not detectable in all other lily tissues examined．The specific expression pattern suggests that LlpCell is confined to lily pollen germination and pollen tube elongation．展开更多
文摘A full—lerlgth cDNA(L／pCe／／)encoding an endo-1，4-β-glucanase(EGase)was isolated from pollen tubes of Lilium longiflorum Thunb．by RT-PCR and RACE．The deduced protein，which iS predicted to be a compact globular protein，is of 490 amino acids．in cluding a putative 21 amino acid-signal peptide．LlpCell shares high level of homology(about50％)with plant secreted EGases，which bear apparently neither trans-membrane domain．nor cellulose—binding domain(CBD)．LIpCell belongs to gycosyl hydrolase family 9．LlpOell transcripts were detected in pollen grains．germinating pollen and elongating pollen tubes with a similar level．whereas LlpCell transcripts were not detectable in all other lily tissues examined．The specific expression pattern suggests that LlpCell is confined to lily pollen germination and pollen tube elongation．
