<strong>Objective:</strong> To investigate the value of the number of circulating tumor cells (CTC) in peripheral blood in the prognosis and coagulation-related indicators of patients with renal cancer. &l...<strong>Objective:</strong> To investigate the value of the number of circulating tumor cells (CTC) in peripheral blood in the prognosis and coagulation-related indicators of patients with renal cancer. <strong>Methods:</strong> 65 patients with renal cell carcinoma (RCC) confirmed pathologically were divided into CTC positive group and CTC negative group according to the CTC count (5 pcs/3.5 ml). Compare the age, gender, tumor location, TNM (clinical stage), pathological grade, tissue type, lymph node metastasis, distant metastasis, prognosis and prothrombin time (PT), fibrinogen (FIB), partial coagulation of the two groups of patients The correlation between the results of zymogen time (APTT) and D-dimer (DD) and the number of CTC. <strong>Results:</strong> There were significant differences in TNM, lymph node metastasis, and distant metastasis between the two groups (P < 0.05). The number of CTC in patients was correlated with FIB and D-D levels (P < 0.05). <strong>Conclusion:</strong> The number of CTC in patients with renal cell carcinoma is correlated with some clinical phenotypes (TNM, lymph node metastasis, distant metastasis) and some coagulation indexes (FIB, D-D), and can jointly predict the prognosis of renal cancer.展开更多
Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysacc...Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysaccharides,sucrose,and phospholipids as well as secondary products(Stasolla et al.,2003;Pareek et al.,2020).Thus,the synthesis of purines is a critical pathway in the cells of all living organisms.Purines can be synthesized through two pathways:de novo and salvage(Stasolla et al.,2003).Recent investigations revealed that purine synthesis is vital for the proper development of chloroplasts in plants.In Arabidopsis,CIA1 encodes the enzyme glutamine phosphoribosyl pyrophosphate amidotransferase,which catalyzes the first committed step of purine de novo biosynthesis,the loss-of-function mutant cia1 shows small,pale-green mosaic leaves(Hung et al.,2004).In rice,both VAL1 and GARS encode glycinamide ribonucleotide synthetase that mediates the second step in purine biosynthesis.展开更多
Improving salt tolerance and mitigating senescence in the presence of high salinity are crucial for sustaining agricultural productivity.Previous research has demonstrated that hydrogen peroxide(H_(2)O_(2)),specifical...Improving salt tolerance and mitigating senescence in the presence of high salinity are crucial for sustaining agricultural productivity.Previous research has demonstrated that hydrogen peroxide(H_(2)O_(2)),specifically H_(2)O_(2)derived from roots and mediated by the respiratory burst oxidase homolog(NADPH),plays a significant role in regulating ion and plant hormone homeostasis in glycophytic plants,such as Arabidopsis.However,the extent to which root-derived H_(2)O_(2)fulfils similar functions in halophytic plants remains uncertain.Therefore,our study aimed to explore the potential contribution of root-sourced H_(2)O_(2)in delaying leaf senescence induced by high salinity,utilizing seashore paspalum as a model halophytic plant.The application of the NADPH-oxidase inhibitor DPI,coupled with a series of leaf senescence analyses,we revealed that root-derived H_(2)O_(2)significantly retards salt-induced leaf senescence.Furthermore,through the application of hormone analysis,lipidomics,ionomics,Non-invasive Micro-test Technology(NMT),and transcriptomics,we established that NADPH-dependent H_(2)O_(2)induced by salt stress in the roots was indispensable for maintaining the balance of the aging hormone,jasmonic acid(JA),and sodium ion homeostasis within this halophytic plant.Finally,by utilizing AtrbohD Arabidopsis mutants and virus-induced gene silencing(VIGs)in Paspalum vaginatum,we demonstrated the pivotal role played by root-sourced H_(2)O_(2)in upholding JA homeostasis and regulating JA-triggered leaf senescence in P.vaginatum.This study offers novel insights into the mechanisms that govern plant leaf senescence and its response to salinity-induced stress.展开更多
Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear....Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.展开更多
Dysphagia is a common complication of stroke,Parkinson's disease(PD),and amyotrophic lateral sclerosis(ALS).The construction of animal models of dysphagia is an important way to explore its pathogenesis and treatm...Dysphagia is a common complication of stroke,Parkinson's disease(PD),and amyotrophic lateral sclerosis(ALS).The construction of animal models of dysphagia is an important way to explore its pathogenesis and treatment.At present,the animal models of dysphagia mainly include rodents,nonhuman primates,and other mammals,such as pigs and dogs.This review systematically summarizes the establishment and evaluation of dysphagia animal models in stroke,PD,and ALS in three kinds of experimental animals,providing a basis for the selection of appropriate animal models of dysphagia.展开更多
In geotechnical engineering,rock bolts are commonly used for reinforcement,while the surrounding rock mass bears varying degrees of shear loads.The shear rate affects the stability of bolted rock joints,especially in ...In geotechnical engineering,rock bolts are commonly used for reinforcement,while the surrounding rock mass bears varying degrees of shear loads.The shear rate affects the stability of bolted rock joints,especially in projects susceptible to dynamic shear loads.In laboratory experiments,fully-grouted bolts and energy-absorbing bolts were used as research objects,and artificial rock specimens with rough joints were fabricated to analyze the shear characteristics and damage mechanisms of bolted rock joints under cyclic shear conditions and different shear velocities.The results showed that as the shear rate increased,the shear strength of bolted rock joint specimens decreased.Degradation of asperities resulted in no obvious peak shear stress in the specimens.Energy-absorbing bolts exhibited greater deformation capacity,with significant necking phenomena and the ability to withstand larger shear displacements.In contrast,fully-grouted bolts,which have threaded surfaces that provide higher bonding performance,exhibited a reduced capacity for plastic deformation and were prone to breaking under smaller shear displacements.Although the shear stiffness of specimens reinforced by energy-absorbing bolts was slightly lower than that of fully-grouted bolt specimens,they demonstrated greater stability under various shear rates.The absorbed shear energy showed that energy-absorbing bolts had superior coordinated deformation capabilities,thus exhibiting greater absorbed shear energy than fully-grouted bolts.Overall,fully-grouted bolts are more suitable for projects requiring higher rock shear strength and overall stiffness.In contrast,energy-absorbing bolts are more suitable for coping with dynamic or fluctuating load conditions to maintain the relative stability of jointed rock masses.展开更多
The reconstruction of demographic history using ancient and modern genomic resources reveals extensive interactions and admixture between ancient nomadic pastoralists and the social organizations of the Chinese Centra...The reconstruction of demographic history using ancient and modern genomic resources reveals extensive interactions and admixture between ancient nomadic pastoralists and the social organizations of the Chinese Central Plain.However,the extent to which Y-chromosome genetic legacies from nomadic emperor-related ancestral lineages influence the Chinese paternal gene pool remains unclear.Here,we genotype 2717 ethnolinguistically diverse samples belonging to C2a lineages,perform whole-genome sequencing on 997 representative samples,and integrate these data with ancient genomic sequences.We reconstruct the evolutionary histories of Northern Zhou-,Qing emperor-,and pastoralist-related lineages to assess their genetic impact on modern Chinese populations.This reassembled fine-scale Ychromosome phylogeny identifies deep divergence and five Neolithic expansion events contributing differently to the formation of northern Chinese populations.Phylogeographic modeling indicates that the nomadic empires of the Northern Zhou and Qing dynasties genetically originated from the Mongolian Plateau.Phylogenetic topology and shared haplotype patterns show that three upstream ancestors of Northern Zhou(C2a1a1b1a2a1b-FGC28857),Donghu tribe(C2a1a1b1-F1756),and Qing(C2a1a3a2-F10283)emperor-related lineages expanded during the middle Neolithic,contributing significantly to genetic flow between ancient northeastern Asians and modern East Asians.Notably,this study reveals limited direct contributions of Emperor Wu of Northern Zhou’s lineages to modern East Asians.展开更多
It was found that the steel plate in the composite plate in the WJ-8 fastener used in high speed rail is rusty. The objective of this study is to test the zinc coating of the steel plate. A literature review was condu...It was found that the steel plate in the composite plate in the WJ-8 fastener used in high speed rail is rusty. The objective of this study is to test the zinc coating of the steel plate. A literature review was conducted to identify the zinc coating techniques, and the companies that can provide different coating service was identified. A salt fog chamber was built that was in compliance with the ANSI B117 code, and the steel plates that were coated by the identified companies were tested using the salt fog chamber. The results indicated that the coating technique that had the best performance in preventing corrosion was the Greenkote plates with passivation. The galvanized option had the roughest coating layer, and it was the most reactive in the salt water solution. This makes it non-ideal for the dynamic rail environment because the increased friction of the plate could damage the supports, especially during extreme temperatures that would cause the rail to expand or contract. Greenkote with Phosphate and ArmorGalv also provided increased corrosion prevention with a smooth, strong finish, but it had more rust on the surface area than the Greenkote with ELU passivation. The ArmorGalv sample had more rust on the surface area than the Greenkote samples. This may not be a weakness in the ArmorGalv process;rather, it likely was the result of this particular sample not having the added protection of a colored coating.展开更多
</span><b><span style="font-family:Verdana;">Objective</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Ve...</span><b><span style="font-family:Verdana;">Objective</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> To investigate the value of CTC clinical features and blood-related test indicators in renal cancer patients by detecting the number of circulating tumor cells (CTC) in patients with renal cancer. <b></span><b><span style="font-family:Verdana;">Methods</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> To analyze 59 patients with renal cell carcinoma (RCC) admitted to the Department of Urology, Affiliated Hospital of Chengde Medical College from May 2018 to October 2019. According to the CTC count (5 pcs/3.5</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ml), they were divided into CTC positive group and CTC negative group. The age, gender, tumor location, tumor size, hematuria, CEC (circulating endothelial cells), CTC Cluster (aggregate), gene mutation, platelet (PLT), albumin (ALB), hemoglobin (HB), alkaline phosphatase (AKP), lactate dehydrogenase (LDH) were compared between the two groups of patients and the correlation of the number of CTCs.</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><b></span><b><span style="font-family:Verdana;">Results</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> There were significant differences in tumor size, CEC, and gene mutations between the two groups (P</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">< 0.05). The number of CTC in patients was correlated with the levels of HB, PLT and LDH (P</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">< 0.05). </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><b></span><b><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">onclusion</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> The number of CTC in RCC patients is correlated with some clinical features (tumor size, CEC, gene mutation) and some related test indicators (HB, PLT, LDH), and can be combined with the above related indicators to predict the occurrence, metastasis and prognosis of renal cancer.展开更多
<strong>Objective:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> T</span></span&...<strong>Objective:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> T</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">he </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">objective </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">is to </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">explore the effects of different surgical methods-retroperitoneal</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> laparoscopic radical nephrectomy (RLRN) and open radical </span><span><span style="font-family:Verdana;">nephrectomy (ORN) on the number of circulating tumor cells (CTC) in the peripheral blood of patients with renal cancer. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> The clinical data of</span></span><span style="font-family:Verdana;"> 63 patients in the Department of Urology, Affiliated Hospital of Chengde Medical College who underwent radical surgery for renal cancer were divided into CTC positive group (18 cases of open surgery and 16 cases of minimally invasive surgery) and CTC negative group (14 cases of open surgery), 15 cases of minimally invasive surgery), overall group (32 cases of open surgery, 31 cases of minimally invasive surgery). Observe the changes in the number of CTC 1 week before operation and 1 week after operation. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> In the positive group, whether it was open surgery or minimally invasive surgery, the postoperative CTC level of patients was significantly reduced (P < 0.05). In the negative group, the CTC decreased significantly after open surgery (P > 0.05), and the CTC level decreased significantly after minimally invasive surgery (P < 0.01). In the overall group, both open and minimally invasive surgery CTC decreased significantly, and the difference was statistically significant (P < 0.05). </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The two different surgical methods can reduce the level of CTC, but compared with ORN, RLRN can significantly reduce the number of postoperative CTC. Patients in the CTC-negative group may be less suitable for open surgery. CTC levels have certain potential in the selection and guidance of treatment modes for patients with renal cell carcinoma (RCC).</span></span></span></span>展开更多
In this paper, the effects of different boron (nitrogen)-doping on the electronic properties of blue phosphorene have been investigated by the first-<span style="font-family:""> <span style=&qu...In this paper, the effects of different boron (nitrogen)-doping on the electronic properties of blue phosphorene have been investigated by the first-<span style="font-family:""> <span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">principles calculations. We have taken eight doping configurations into account, the calculated results show that the bond length of P-B is decreasing with the doping concentration increasing. For the four boron atoms doping configuration, the geometric structure appears the distinct distortion. The band gap is decreasing with the doping concentration increasing, and it appears the transition from indirect band gap to direct band gap for boron doping configurations. It is hoped that the calculated results may be useful for designing electronic devices based on blue phosphorene.展开更多
Objective:The tyrosine phosphatase SHP2 has a dual role in cancer initiation and progression in a tissue type-dependent manner.Several studies have linked SHP2 to the aggressive behavior of breast cancer cells and poo...Objective:The tyrosine phosphatase SHP2 has a dual role in cancer initiation and progression in a tissue type-dependent manner.Several studies have linked SHP2 to the aggressive behavior of breast cancer cells and poorer outcomes in people with cancer.Nevertheless,the mechanistic details of how SHP2 promotes breast cancer progression remain largely undefined.Methods:The relationship between SHP2 expression and the prognosis of patients with breast cancer was investigated by using the TCGA and GEO databases.The expression of SHP2 in breast cancer tissues was analyzed by immunohistochemistry.CRISPR/Cas9 technology was used to generate SHP2-knockout breast cancer cells.Cell-counting kit-8,colony formation,cell cycle,and EdU incorporation assays,as well as a tumor xenograft model were used to examine the function of SHP2 in breast cancer proliferation.Quantitative RT-PCR,western blotting,immunofluorescence staining,and ubiquitination assays were used to explore the molecular mechanism through which SHP2 regulates breast cancer proliferation.Results:High SHP2 expression is correlated with poor prognosis in patients with breast cancer.SHP2 is required for the proliferation of breast cancer cellsin vitro and tumor growthin vivo through regulation of Cyclin D1 abundance,thereby accelerating cell cycle progression.Notably,SHP2 modulates the ubiquitin–proteasome-dependent degradation of Cyclin D1viathe PI3K/AKT/GSK3βsignaling pathway.SHP2 knockout attenuates the activation of PI3K/AKT signaling and causes the dephosphorylation and resultant activation of GSK3β.GSK3βthen mediates phosphorylation of Cyclin D1 at threonine 286,thereby promoting the translocation of Cyclin D1 from the nucleus to the cytoplasm and facilitating Cyclin D1 degradation through the ubiquitin–proteasome system.Conclusions:Our study uncovered the mechanism through which SHP2 regulates breast cancer proliferation.SHP2 may therefore potentially serve as a therapeutic target for breast cancer.展开更多
The lineage specification of mesenchymal stem/stromal cells(MSCs) is tightly regulated by a wide range of factors. Recently, the versatile functions of ZBP1(also known as DAI or DLM-1) have been reported in the blood ...The lineage specification of mesenchymal stem/stromal cells(MSCs) is tightly regulated by a wide range of factors. Recently, the versatile functions of ZBP1(also known as DAI or DLM-1) have been reported in the blood circulation and immune systems.However, the biological function of ZBP1 during the lineage specification of MSCs is still unknown. In the present study, we found that ZBP1 was upregulated during osteogenesis but downregulated during adipogenesis in mouse bone marrow-derived MSCs(m BMSCs). ZBP1 was highly expressed in osteoblasts but expressed at a relatively low level in marrow adipocytes. Knockdown of ZBP1 inhibited alkaline phosphataseactivity, extracellular matrix mineralization, and osteogenesis-related gene expression in vitro and reduced ectopic bone formation in vivo. Knockdown of ZBP1 also promoted adipogenesis in MSCs in vitro. Conversely, the overexpression of ZBP1 increased the osteogenesis but suppressed the adipogenesis of MSCs. When the expression of ZBP1 was rescued, the osteogenic capacity of ZBP1-depleted m BMSCs was restored at both the molecular and phenotypic levels.Furthermore, we demonstrated that ZBP1, a newly identified target of Wnt/β-catenin signaling, was required for β-catenin translocation into nuclei. Collectively, our results indicate that ZBP1 is a novel regulator of bone and fat transdifferentiation via Wnt/β-catenin signaling.展开更多
文摘<strong>Objective:</strong> To investigate the value of the number of circulating tumor cells (CTC) in peripheral blood in the prognosis and coagulation-related indicators of patients with renal cancer. <strong>Methods:</strong> 65 patients with renal cell carcinoma (RCC) confirmed pathologically were divided into CTC positive group and CTC negative group according to the CTC count (5 pcs/3.5 ml). Compare the age, gender, tumor location, TNM (clinical stage), pathological grade, tissue type, lymph node metastasis, distant metastasis, prognosis and prothrombin time (PT), fibrinogen (FIB), partial coagulation of the two groups of patients The correlation between the results of zymogen time (APTT) and D-dimer (DD) and the number of CTC. <strong>Results:</strong> There were significant differences in TNM, lymph node metastasis, and distant metastasis between the two groups (P < 0.05). The number of CTC in patients was correlated with FIB and D-D levels (P < 0.05). <strong>Conclusion:</strong> The number of CTC in patients with renal cell carcinoma is correlated with some clinical phenotypes (TNM, lymph node metastasis, distant metastasis) and some coagulation indexes (FIB, D-D), and can jointly predict the prognosis of renal cancer.
基金funded by the National Natural Science Foundation of China(Grant No.32202485)the Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences(Grant No.2024JQ02)+1 种基金the Zhongyuan Sci-Tech Innovation Leading Talents(Grant No.244200510041)the Key SciTech R&D Project of Joint Foundation in Henan Province(Grant No.232301420024).
文摘Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysaccharides,sucrose,and phospholipids as well as secondary products(Stasolla et al.,2003;Pareek et al.,2020).Thus,the synthesis of purines is a critical pathway in the cells of all living organisms.Purines can be synthesized through two pathways:de novo and salvage(Stasolla et al.,2003).Recent investigations revealed that purine synthesis is vital for the proper development of chloroplasts in plants.In Arabidopsis,CIA1 encodes the enzyme glutamine phosphoribosyl pyrophosphate amidotransferase,which catalyzes the first committed step of purine de novo biosynthesis,the loss-of-function mutant cia1 shows small,pale-green mosaic leaves(Hung et al.,2004).In rice,both VAL1 and GARS encode glycinamide ribonucleotide synthetase that mediates the second step in purine biosynthesis.
基金supported by the Project funded by the Natural Science Foundation of Hainan Province(Grant No.322QN248)the National Natural Science Foundation of China(Grant Nos.32401488,32060409,32371782 and 32460358)+3 种基金the Innovational Fund for Scientific and Technological Personnel of Hainan Province(Grant No.KJRC 2023C21)the Hainan High-level Talents Project(Grant No.321RC475)Collaborative Innovation Center Project of Nanfan and High-Efficiency Tropical Agriculture in Hainan University(XTCX2022NYB08)Collaborative Innovation Center Project of Ecological Civilization in Hainan University(XTCX2022STC10).
文摘Improving salt tolerance and mitigating senescence in the presence of high salinity are crucial for sustaining agricultural productivity.Previous research has demonstrated that hydrogen peroxide(H_(2)O_(2)),specifically H_(2)O_(2)derived from roots and mediated by the respiratory burst oxidase homolog(NADPH),plays a significant role in regulating ion and plant hormone homeostasis in glycophytic plants,such as Arabidopsis.However,the extent to which root-derived H_(2)O_(2)fulfils similar functions in halophytic plants remains uncertain.Therefore,our study aimed to explore the potential contribution of root-sourced H_(2)O_(2)in delaying leaf senescence induced by high salinity,utilizing seashore paspalum as a model halophytic plant.The application of the NADPH-oxidase inhibitor DPI,coupled with a series of leaf senescence analyses,we revealed that root-derived H_(2)O_(2)significantly retards salt-induced leaf senescence.Furthermore,through the application of hormone analysis,lipidomics,ionomics,Non-invasive Micro-test Technology(NMT),and transcriptomics,we established that NADPH-dependent H_(2)O_(2)induced by salt stress in the roots was indispensable for maintaining the balance of the aging hormone,jasmonic acid(JA),and sodium ion homeostasis within this halophytic plant.Finally,by utilizing AtrbohD Arabidopsis mutants and virus-induced gene silencing(VIGs)in Paspalum vaginatum,we demonstrated the pivotal role played by root-sourced H_(2)O_(2)in upholding JA homeostasis and regulating JA-triggered leaf senescence in P.vaginatum.This study offers novel insights into the mechanisms that govern plant leaf senescence and its response to salinity-induced stress.
基金supported by the China Agricultural Research System(CARS-23-G15)the Innovation Team of Henan Academy of Agricultural Sciences,China(2024TD06)+2 种基金the Autonomous Innovation Project of Henan Academy of Agricultural Sciences,China(2024ZC034)the Joint Research on Agricultural Variety Improvement of Henan Province,China(2022010504)the Key Research&Development Project of Henan Province,China(221111110100)。
文摘Ogura cytoplasmic male sterility(Ogura CMS)was first identified in wild radish(Raphanus sativus)and resulted in complete pollen abortion.However,the molecular mechanism of Ogura CMS in Chinese cabbage remains unclear.A cytological analysis confirmed nuclear degradation during the late uninucleate stage of pollen development,which diminished by the tricellular stage.Concurrently,tapetal cells exhibited abnormal enlargement and vacuolation starting from the tetrad stage.Serious developmental defects were observed in the pollen wall.During early pollen development,genes associated with cytochrome c and programmed cell death(PCD)were upregulated in the Ogura CMS line,while genes involved in pollen wall mitosis were downregulated.Conversely,at the late stage of pollen development,peroxisome and autophagy-related genes in the Ogura CMS line were upregulated.The mitochondrial orf138 gene mutation triggered the PCD process in tapetal cells,leading to their abnormal enlargement and the degradation of their contents,eventually resulting in vacuolation at the tricellular stage.These tapetal defects hindered the provision of adequate sporopollenin and nutrients to the microspores,consequently leading to abnormal pollen wall development and abnormal mitosis in the microspores.Ultimately,nuclear dispersion commenced during the late uninucleate stage,and autophagy occurred in the late stage of pollen development.Consequently,the plant could not produce functional pollen,resulting in male sterility in Chinese cabbage.Studies of Ogura CMS can promote the production and application of male sterile materials and enrich male sterile resources,which is of great significance for hybrid breeding.
基金National Natural Science Foundation of China,Grant/Award Number:82172531Joint Funds for the Innovation of Science and Technology,Fujian Province,Grant/Award Number:2021Y9105。
文摘Dysphagia is a common complication of stroke,Parkinson's disease(PD),and amyotrophic lateral sclerosis(ALS).The construction of animal models of dysphagia is an important way to explore its pathogenesis and treatment.At present,the animal models of dysphagia mainly include rodents,nonhuman primates,and other mammals,such as pigs and dogs.This review systematically summarizes the establishment and evaluation of dysphagia animal models in stroke,PD,and ALS in three kinds of experimental animals,providing a basis for the selection of appropriate animal models of dysphagia.
基金partially funded by the National Natural Science Foundation of China(Grant Nos.52179098 and 41907251)the State Scholarship Fund of China(Grant No.202306650001).
文摘In geotechnical engineering,rock bolts are commonly used for reinforcement,while the surrounding rock mass bears varying degrees of shear loads.The shear rate affects the stability of bolted rock joints,especially in projects susceptible to dynamic shear loads.In laboratory experiments,fully-grouted bolts and energy-absorbing bolts were used as research objects,and artificial rock specimens with rough joints were fabricated to analyze the shear characteristics and damage mechanisms of bolted rock joints under cyclic shear conditions and different shear velocities.The results showed that as the shear rate increased,the shear strength of bolted rock joint specimens decreased.Degradation of asperities resulted in no obvious peak shear stress in the specimens.Energy-absorbing bolts exhibited greater deformation capacity,with significant necking phenomena and the ability to withstand larger shear displacements.In contrast,fully-grouted bolts,which have threaded surfaces that provide higher bonding performance,exhibited a reduced capacity for plastic deformation and were prone to breaking under smaller shear displacements.Although the shear stiffness of specimens reinforced by energy-absorbing bolts was slightly lower than that of fully-grouted bolt specimens,they demonstrated greater stability under various shear rates.The absorbed shear energy showed that energy-absorbing bolts had superior coordinated deformation capabilities,thus exhibiting greater absorbed shear energy than fully-grouted bolts.Overall,fully-grouted bolts are more suitable for projects requiring higher rock shear strength and overall stiffness.In contrast,energy-absorbing bolts are more suitable for coping with dynamic or fluctuating load conditions to maintain the relative stability of jointed rock masses.
基金the financial support received from the National Natural Science Foundation of China(82202078)the National Social Science Foundation of China(23&ZD203)+4 种基金support for G.H.includes National Natural Science Foundation of China(82402203)the Open Project of the Key Laboratory of Forensic Genetics of the Ministry of Public Security(2022FGKFKT05)the Center for Archaeological Science of Sichuan University(23SASA01)the 1‧3‧5 Project for Disciplines of Excellence at West China Hospital,Sichuan University(ZYJC20002)the Sichuan Science and Technology Program(2024NSFSC1518).
文摘The reconstruction of demographic history using ancient and modern genomic resources reveals extensive interactions and admixture between ancient nomadic pastoralists and the social organizations of the Chinese Central Plain.However,the extent to which Y-chromosome genetic legacies from nomadic emperor-related ancestral lineages influence the Chinese paternal gene pool remains unclear.Here,we genotype 2717 ethnolinguistically diverse samples belonging to C2a lineages,perform whole-genome sequencing on 997 representative samples,and integrate these data with ancient genomic sequences.We reconstruct the evolutionary histories of Northern Zhou-,Qing emperor-,and pastoralist-related lineages to assess their genetic impact on modern Chinese populations.This reassembled fine-scale Ychromosome phylogeny identifies deep divergence and five Neolithic expansion events contributing differently to the formation of northern Chinese populations.Phylogeographic modeling indicates that the nomadic empires of the Northern Zhou and Qing dynasties genetically originated from the Mongolian Plateau.Phylogenetic topology and shared haplotype patterns show that three upstream ancestors of Northern Zhou(C2a1a1b1a2a1b-FGC28857),Donghu tribe(C2a1a1b1-F1756),and Qing(C2a1a3a2-F10283)emperor-related lineages expanded during the middle Neolithic,contributing significantly to genetic flow between ancient northeastern Asians and modern East Asians.Notably,this study reveals limited direct contributions of Emperor Wu of Northern Zhou’s lineages to modern East Asians.
文摘It was found that the steel plate in the composite plate in the WJ-8 fastener used in high speed rail is rusty. The objective of this study is to test the zinc coating of the steel plate. A literature review was conducted to identify the zinc coating techniques, and the companies that can provide different coating service was identified. A salt fog chamber was built that was in compliance with the ANSI B117 code, and the steel plates that were coated by the identified companies were tested using the salt fog chamber. The results indicated that the coating technique that had the best performance in preventing corrosion was the Greenkote plates with passivation. The galvanized option had the roughest coating layer, and it was the most reactive in the salt water solution. This makes it non-ideal for the dynamic rail environment because the increased friction of the plate could damage the supports, especially during extreme temperatures that would cause the rail to expand or contract. Greenkote with Phosphate and ArmorGalv also provided increased corrosion prevention with a smooth, strong finish, but it had more rust on the surface area than the Greenkote with ELU passivation. The ArmorGalv sample had more rust on the surface area than the Greenkote samples. This may not be a weakness in the ArmorGalv process;rather, it likely was the result of this particular sample not having the added protection of a colored coating.
文摘</span><b><span style="font-family:Verdana;">Objective</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> To investigate the value of CTC clinical features and blood-related test indicators in renal cancer patients by detecting the number of circulating tumor cells (CTC) in patients with renal cancer. <b></span><b><span style="font-family:Verdana;">Methods</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> To analyze 59 patients with renal cell carcinoma (RCC) admitted to the Department of Urology, Affiliated Hospital of Chengde Medical College from May 2018 to October 2019. According to the CTC count (5 pcs/3.5</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ml), they were divided into CTC positive group and CTC negative group. The age, gender, tumor location, tumor size, hematuria, CEC (circulating endothelial cells), CTC Cluster (aggregate), gene mutation, platelet (PLT), albumin (ALB), hemoglobin (HB), alkaline phosphatase (AKP), lactate dehydrogenase (LDH) were compared between the two groups of patients and the correlation of the number of CTCs.</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><b></span><b><span style="font-family:Verdana;">Results</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> There were significant differences in tumor size, CEC, and gene mutations between the two groups (P</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">< 0.05). The number of CTC in patients was correlated with the levels of HB, PLT and LDH (P</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">< 0.05). </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"><b></span><b><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">onclusion</span></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">:</span></b></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"></b></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> The number of CTC in RCC patients is correlated with some clinical features (tumor size, CEC, gene mutation) and some related test indicators (HB, PLT, LDH), and can be combined with the above related indicators to predict the occurrence, metastasis and prognosis of renal cancer.
文摘<strong>Objective:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> T</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">he </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">objective </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">is to </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">explore the effects of different surgical methods-retroperitoneal</span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;"> laparoscopic radical nephrectomy (RLRN) and open radical </span><span><span style="font-family:Verdana;">nephrectomy (ORN) on the number of circulating tumor cells (CTC) in the peripheral blood of patients with renal cancer. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> The clinical data of</span></span><span style="font-family:Verdana;"> 63 patients in the Department of Urology, Affiliated Hospital of Chengde Medical College who underwent radical surgery for renal cancer were divided into CTC positive group (18 cases of open surgery and 16 cases of minimally invasive surgery) and CTC negative group (14 cases of open surgery), 15 cases of minimally invasive surgery), overall group (32 cases of open surgery, 31 cases of minimally invasive surgery). Observe the changes in the number of CTC 1 week before operation and 1 week after operation. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> In the positive group, whether it was open surgery or minimally invasive surgery, the postoperative CTC level of patients was significantly reduced (P < 0.05). In the negative group, the CTC decreased significantly after open surgery (P > 0.05), and the CTC level decreased significantly after minimally invasive surgery (P < 0.01). In the overall group, both open and minimally invasive surgery CTC decreased significantly, and the difference was statistically significant (P < 0.05). </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The two different surgical methods can reduce the level of CTC, but compared with ORN, RLRN can significantly reduce the number of postoperative CTC. Patients in the CTC-negative group may be less suitable for open surgery. CTC levels have certain potential in the selection and guidance of treatment modes for patients with renal cell carcinoma (RCC).</span></span></span></span>
文摘In this paper, the effects of different boron (nitrogen)-doping on the electronic properties of blue phosphorene have been investigated by the first-<span style="font-family:""> <span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">principles calculations. We have taken eight doping configurations into account, the calculated results show that the bond length of P-B is decreasing with the doping concentration increasing. For the four boron atoms doping configuration, the geometric structure appears the distinct distortion. The band gap is decreasing with the doping concentration increasing, and it appears the transition from indirect band gap to direct band gap for boron doping configurations. It is hoped that the calculated results may be useful for designing electronic devices based on blue phosphorene.
基金This work was supported by grants from the National Natural S&ence Foundation of China(grant Nos.81372844,81472474,81772804 and 81903092)Tianjin Municipal Science and Technology Commission(grant No.16JCYBJC25400)+1 种基金Changjiang Researchers and Innovative Research Team(grant No.IRT_14R40)Postgraduate Innovation Fund of"13th Five-Year Comprehensive Investment,"Tianjin Medical University(grant No.YJSCX201716).
文摘Objective:The tyrosine phosphatase SHP2 has a dual role in cancer initiation and progression in a tissue type-dependent manner.Several studies have linked SHP2 to the aggressive behavior of breast cancer cells and poorer outcomes in people with cancer.Nevertheless,the mechanistic details of how SHP2 promotes breast cancer progression remain largely undefined.Methods:The relationship between SHP2 expression and the prognosis of patients with breast cancer was investigated by using the TCGA and GEO databases.The expression of SHP2 in breast cancer tissues was analyzed by immunohistochemistry.CRISPR/Cas9 technology was used to generate SHP2-knockout breast cancer cells.Cell-counting kit-8,colony formation,cell cycle,and EdU incorporation assays,as well as a tumor xenograft model were used to examine the function of SHP2 in breast cancer proliferation.Quantitative RT-PCR,western blotting,immunofluorescence staining,and ubiquitination assays were used to explore the molecular mechanism through which SHP2 regulates breast cancer proliferation.Results:High SHP2 expression is correlated with poor prognosis in patients with breast cancer.SHP2 is required for the proliferation of breast cancer cellsin vitro and tumor growthin vivo through regulation of Cyclin D1 abundance,thereby accelerating cell cycle progression.Notably,SHP2 modulates the ubiquitin–proteasome-dependent degradation of Cyclin D1viathe PI3K/AKT/GSK3βsignaling pathway.SHP2 knockout attenuates the activation of PI3K/AKT signaling and causes the dephosphorylation and resultant activation of GSK3β.GSK3βthen mediates phosphorylation of Cyclin D1 at threonine 286,thereby promoting the translocation of Cyclin D1 from the nucleus to the cytoplasm and facilitating Cyclin D1 degradation through the ubiquitin–proteasome system.Conclusions:Our study uncovered the mechanism through which SHP2 regulates breast cancer proliferation.SHP2 may therefore potentially serve as a therapeutic target for breast cancer.
基金supported by the Foundation of the National Natural Science Foundation of China (No. 81671024, 81371171, 81571009, and 81600877)the China Postdoctoral Science Foundation (2016M600745)。
文摘The lineage specification of mesenchymal stem/stromal cells(MSCs) is tightly regulated by a wide range of factors. Recently, the versatile functions of ZBP1(also known as DAI or DLM-1) have been reported in the blood circulation and immune systems.However, the biological function of ZBP1 during the lineage specification of MSCs is still unknown. In the present study, we found that ZBP1 was upregulated during osteogenesis but downregulated during adipogenesis in mouse bone marrow-derived MSCs(m BMSCs). ZBP1 was highly expressed in osteoblasts but expressed at a relatively low level in marrow adipocytes. Knockdown of ZBP1 inhibited alkaline phosphataseactivity, extracellular matrix mineralization, and osteogenesis-related gene expression in vitro and reduced ectopic bone formation in vivo. Knockdown of ZBP1 also promoted adipogenesis in MSCs in vitro. Conversely, the overexpression of ZBP1 increased the osteogenesis but suppressed the adipogenesis of MSCs. When the expression of ZBP1 was rescued, the osteogenic capacity of ZBP1-depleted m BMSCs was restored at both the molecular and phenotypic levels.Furthermore, we demonstrated that ZBP1, a newly identified target of Wnt/β-catenin signaling, was required for β-catenin translocation into nuclei. Collectively, our results indicate that ZBP1 is a novel regulator of bone and fat transdifferentiation via Wnt/β-catenin signaling.
