The doubled-haploid technique mediated by parthenogenesis and androgenesis in plants can directly generate homozygous diploid lines after chromosome doubling with colchicine in one or two generations,and this method u...The doubled-haploid technique mediated by parthenogenesis and androgenesis in plants can directly generate homozygous diploid lines after chromosome doubling with colchicine in one or two generations,and this method undoubtedly shortens the breeding process and improves breeding efficiency in crops.Previously,haploid plants were mainly induced by anther culture or microspore culture via androgenesis(Ohnoutkova et al.,2019).In wheat(Triticum aestivum),haploids can also be induced by maize(Zea mays)pollen and chromosome elimination via parthenogenesis(Liu et al.,2020b).However,the aforementioned induction methods are genotype-dependent,require complex manipulations,and are both time-consuming and inefficient.Therefore,it is necessary to develop new techniques or germplasm for simple and efficient haploid induction.展开更多
Dear Editor,Breeding a new wheat variety using traditional methods typically takes at least 8 to 10 years,and the breeding period can be dramatically shortened via a doubled haploid strategy,which can yield homozygote...Dear Editor,Breeding a new wheat variety using traditional methods typically takes at least 8 to 10 years,and the breeding period can be dramatically shortened via a doubled haploid strategy,which can yield homozygotes within one or two generations.In the past several decades,wheat haploids have been widely induced through anther or microspore culture and chromosome elimination via interspecific hybridization between wheat and maize.The first technique exhibits strong genotype dependency and a severe albino phenomenon,whereas the latter shows low induction efficiency(Sangam et al.,2015).In addition,haploid induction procedures using the aforementioned methods are complicated to perform and require specialized equipment and environmentally controlled conditions.Thus,the application of these technologies in wheat breeding has been limited(Sangam et al.,2015).展开更多
基金supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(S2021ZD03 and 2060302-2-19).
文摘The doubled-haploid technique mediated by parthenogenesis and androgenesis in plants can directly generate homozygous diploid lines after chromosome doubling with colchicine in one or two generations,and this method undoubtedly shortens the breeding process and improves breeding efficiency in crops.Previously,haploid plants were mainly induced by anther culture or microspore culture via androgenesis(Ohnoutkova et al.,2019).In wheat(Triticum aestivum),haploids can also be induced by maize(Zea mays)pollen and chromosome elimination via parthenogenesis(Liu et al.,2020b).However,the aforementioned induction methods are genotype-dependent,require complex manipulations,and are both time-consuming and inefficient.Therefore,it is necessary to develop new techniques or germplasm for simple and efficient haploid induction.
基金supported by grants from the National Natural Science Foundation of China(grant no.32272180)the Key Research and Development Program from the Science and Technology Department of Ningxia Hui Autonomous Region(grant no.2022BBF02039).
文摘Dear Editor,Breeding a new wheat variety using traditional methods typically takes at least 8 to 10 years,and the breeding period can be dramatically shortened via a doubled haploid strategy,which can yield homozygotes within one or two generations.In the past several decades,wheat haploids have been widely induced through anther or microspore culture and chromosome elimination via interspecific hybridization between wheat and maize.The first technique exhibits strong genotype dependency and a severe albino phenomenon,whereas the latter shows low induction efficiency(Sangam et al.,2015).In addition,haploid induction procedures using the aforementioned methods are complicated to perform and require specialized equipment and environmentally controlled conditions.Thus,the application of these technologies in wheat breeding has been limited(Sangam et al.,2015).
