Ultrasensitive and simultaneous quantitative detection of Listeria monocytogenes(L.monocytogenes)that can survive in vacuum packaging and frozen food is critical to public health.Herein,teamed boronate affinity(TBA)ma...Ultrasensitive and simultaneous quantitative detection of Listeria monocytogenes(L.monocytogenes)that can survive in vacuum packaging and frozen food is critical to public health.Herein,teamed boronate affinity(TBA)magnetically functionalized materials combined with biomass fluorescent probe for L.monocytogenes detection in a sandwich-like strategy was established.Firstly,we prepared new TBA magnetically functionalized materials by reducing the pKa value of phenylboronic acid through the TBA strategy in the form of self-assembled intermolecular B-N coordination bonding.To our knowledge,this is the first study where TBA magnetically functionalized materials were utilized for the identification and capture of bacteria,with a high capture efficiency of over 90.8%in spiked lettuce samples for L.monocytogenes(within 35 min),showing superior capture ability.Then,Anti-L.monocytogenes monoclonal antibody was labeled in biomass-derived fluorescent probe with the help of biotin-streptavidin system to achieve specific and sensitive detection of L.monocytogenes.The sandwich-like strategy shows high sensitivity of designed fluorescence sensing platform for L.monocytogenes with a wide linear range(2.2×10^(1)-2.2×10^(6)CFU/mL),a low limit of detection(2.2×10^(1)CFU/mL),and a satisfactory recovery rate(85.19%-106.69%)spiked lettuce sample.Notably,the method was low-cost,general and had strong application prospects in the field of food safety.展开更多
This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the ...This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the world,posing a serious threat to food safety and public health.Therefore,the detection and effective elimination of L.monocytogenes is significant for ensuring food safety and preventing secondary contamination.Herein,a bifunctional gold carbon dots-silver nanoclusters(GCDs-AgNCs)nanocomposite with excellent fluorescence properties and antimicrobial activity was synthesized in this study.On the one hand,a fluorescence biosensor for the detection of L.monocytogenes by sandwich method was constructed with magnetic separation technique and aptamer-modified GCDs-AgNCs.The biosensor exhibited great detection performance for L.monocytogenes in spiked samples with a limit of detection of 160 CFU/mL.On the other hand,GCDs-AgNCs with antimicrobial activity can eliminate L.monocytogenes by destroying the bacterial cell structure and affecting bacterial metabolism,which effectively avoids secondary contamination.Therefore,this bifunctional nanomaterial can provide a referable new strategy for the rapid analysis and effective elimination of foodborne pathogens.展开更多
基金the Research Project of State Key Laboratory of Food Science and Resources,Nanchang University(Project No.SKLF-ZZB-202328)for financial support.
文摘Ultrasensitive and simultaneous quantitative detection of Listeria monocytogenes(L.monocytogenes)that can survive in vacuum packaging and frozen food is critical to public health.Herein,teamed boronate affinity(TBA)magnetically functionalized materials combined with biomass fluorescent probe for L.monocytogenes detection in a sandwich-like strategy was established.Firstly,we prepared new TBA magnetically functionalized materials by reducing the pKa value of phenylboronic acid through the TBA strategy in the form of self-assembled intermolecular B-N coordination bonding.To our knowledge,this is the first study where TBA magnetically functionalized materials were utilized for the identification and capture of bacteria,with a high capture efficiency of over 90.8%in spiked lettuce samples for L.monocytogenes(within 35 min),showing superior capture ability.Then,Anti-L.monocytogenes monoclonal antibody was labeled in biomass-derived fluorescent probe with the help of biotin-streptavidin system to achieve specific and sensitive detection of L.monocytogenes.The sandwich-like strategy shows high sensitivity of designed fluorescence sensing platform for L.monocytogenes with a wide linear range(2.2×10^(1)-2.2×10^(6)CFU/mL),a low limit of detection(2.2×10^(1)CFU/mL),and a satisfactory recovery rate(85.19%-106.69%)spiked lettuce sample.Notably,the method was low-cost,general and had strong application prospects in the field of food safety.
基金Research Project of State Key Laboratory of Food Science and Resources,Nanchang University(Project No.SKLF-ZZB-202328)for financial support.
文摘This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the world,posing a serious threat to food safety and public health.Therefore,the detection and effective elimination of L.monocytogenes is significant for ensuring food safety and preventing secondary contamination.Herein,a bifunctional gold carbon dots-silver nanoclusters(GCDs-AgNCs)nanocomposite with excellent fluorescence properties and antimicrobial activity was synthesized in this study.On the one hand,a fluorescence biosensor for the detection of L.monocytogenes by sandwich method was constructed with magnetic separation technique and aptamer-modified GCDs-AgNCs.The biosensor exhibited great detection performance for L.monocytogenes in spiked samples with a limit of detection of 160 CFU/mL.On the other hand,GCDs-AgNCs with antimicrobial activity can eliminate L.monocytogenes by destroying the bacterial cell structure and affecting bacterial metabolism,which effectively avoids secondary contamination.Therefore,this bifunctional nanomaterial can provide a referable new strategy for the rapid analysis and effective elimination of foodborne pathogens.
