The use of zinc oxide(ZnO)photosensitizers(PSs)-mediated photodynamic therapy(PDT)against bac-terial wound infections is greatly restricted by diminished photocatalytic efficiency caused by the rapid recombination of ...The use of zinc oxide(ZnO)photosensitizers(PSs)-mediated photodynamic therapy(PDT)against bac-terial wound infections is greatly restricted by diminished photocatalytic efficiency caused by the rapid recombination of photogenerated electrons and holes.In this work,ZnO PSs with a Ni-doped/oxygen vacancy and a protective carbon shell were successfully synthesized by calcinating a Ni-doping zeolitic imidazolate framework-8 precursor.The double-defect structure and the carbon-based substrates signif-icantly promoted the efficiency of photogenerated electron-hole pair separation,meanwhile,the 3%Ni doping endows it with great photocatalytic performance as elucidated by photodegradation assays of methylene blue(MB)and density functional theory(DFT)calculations,reinforcing the generation of reactive oxygen species(ROS)by ZnO and showing obvious advantages in antibacterial properties.As the enhanced photogenerated electron transfer and the ROS damage underwent localized accumulation,the PSs disturbed the bacterial membrane integrity and caused bacterial ATP synthesis inhibition,fur-ther leading to bacterial lysis and promoting bacterial deaths.Additionally,the PSs showed outstanding efficacy in eradicating bacterial biofilms.Simultaneously,the significantly enhanced PDT antibacterial per-formance of the PSs in vivo could initiate wound tissue repair and trigger anti-inflammatory reactions by significantly regulating the expression levels of regeneration-and inflammation-related genes or proteins.Furthermore,the PSs consistently exhibited favorable compatibility both in vitro and in vivo.In summary,this study offers evidence of the remarkably efficient and biologically safe performance of Ni-ZnO@C PSs,with antibacterial properties advancing wound healing,both in controlled laboratory environments and living organisms,further underscoring their substantial potential for biomedical applications.展开更多
An MYB transcription factor (AtMYB305) of Arabidopsis was structurally characterized. The biochemical activity of the protein was tested and its in vivo function was preliminarily analyzed. AtMYB305 contains two imper...An MYB transcription factor (AtMYB305) of Arabidopsis was structurally characterized. The biochemical activity of the protein was tested and its in vivo function was preliminarily analyzed. AtMYB305 contains two imperfect repeats (R2, R3) in its DNA binding domain. Gel mobility shift assay demonstrated that GST-AtMYB305 fusion pro-tein bound specifically to the DNA fragment that included a consensus MYB recognition sequence (TAACTG). Overex-pression of AtMYB305 in the fission yeast caused the forma-tion of elongated cells with one condensed nucleus. Semi-quantitative RT-PCR analysis revealed that AtMYB305 was expressed specifically in the roots of Arabidopsis.展开更多
Spun-bond non-woven fabrics(NWFs)made of porous C-shaped polypropylene fibers were applied in rapid oil absorption and effective on-line oil spillage monitoring.It is of great interest to further optimize the absorpti...Spun-bond non-woven fabrics(NWFs)made of porous C-shaped polypropylene fibers were applied in rapid oil absorption and effective on-line oil spillage monitoring.It is of great interest to further optimize the absorption properties of these materials by tuning their preparation parameters as well as characterize them with theoretical models.In this paper,effects of die shape,diluent composition(mixtures of dibutyl and dioctyl phthalate),and drawing speed on their porous structure and oil-absorbing performance were systematically investigated and characterized based on two novel concepts,i.e.,the equivalent capillary tube pore radius and the kinetic pore tortuosity(barrier to access)derived from the simplest capillary tube liquid-filling model.The use of higher dibutyl phthalate fractions under faster drawing speeds resulted in the formation of larger and more connected inner filament sub-micron pores.Three stages of tube filling relating to inter-filament large pores,medium pores close to bonding points,and inner filament small pores were observed in the spun-bond NWFs.Continuous oil recovery rates of 986 L·m^(-2)·h^(-1) with an oil/water selectivity of 6.4 were achieved in dynamic skimming experiments using simulated spilled oil.展开更多
基金financially supported by the National Natu-ral Science Foundation of China(Nos.82060386,82272440,and 22365013)the Hainan Province Science and Technology Spe-cial Fund(Nos.ZDKJ2021029 and ZDYF2021SHFZ068)+4 种基金the Hainan Provincial Natural Science Foundation of China(No.823CXTD376)the CAMS Innovation Fund for Medical Sciences(No.2019-I2M-5-023)the Key Laboratory Open Project Fund of Emergency and Trauma of Ministry of Education(No.KLET-202008)the Education Department of Hainan Province(Nos.Qhyb2022-131 and Qhys2022-281)Hainan Province Clinical Medical Center.
文摘The use of zinc oxide(ZnO)photosensitizers(PSs)-mediated photodynamic therapy(PDT)against bac-terial wound infections is greatly restricted by diminished photocatalytic efficiency caused by the rapid recombination of photogenerated electrons and holes.In this work,ZnO PSs with a Ni-doped/oxygen vacancy and a protective carbon shell were successfully synthesized by calcinating a Ni-doping zeolitic imidazolate framework-8 precursor.The double-defect structure and the carbon-based substrates signif-icantly promoted the efficiency of photogenerated electron-hole pair separation,meanwhile,the 3%Ni doping endows it with great photocatalytic performance as elucidated by photodegradation assays of methylene blue(MB)and density functional theory(DFT)calculations,reinforcing the generation of reactive oxygen species(ROS)by ZnO and showing obvious advantages in antibacterial properties.As the enhanced photogenerated electron transfer and the ROS damage underwent localized accumulation,the PSs disturbed the bacterial membrane integrity and caused bacterial ATP synthesis inhibition,fur-ther leading to bacterial lysis and promoting bacterial deaths.Additionally,the PSs showed outstanding efficacy in eradicating bacterial biofilms.Simultaneously,the significantly enhanced PDT antibacterial per-formance of the PSs in vivo could initiate wound tissue repair and trigger anti-inflammatory reactions by significantly regulating the expression levels of regeneration-and inflammation-related genes or proteins.Furthermore,the PSs consistently exhibited favorable compatibility both in vitro and in vivo.In summary,this study offers evidence of the remarkably efficient and biologically safe performance of Ni-ZnO@C PSs,with antibacterial properties advancing wound healing,both in controlled laboratory environments and living organisms,further underscoring their substantial potential for biomedical applications.
文摘An MYB transcription factor (AtMYB305) of Arabidopsis was structurally characterized. The biochemical activity of the protein was tested and its in vivo function was preliminarily analyzed. AtMYB305 contains two imperfect repeats (R2, R3) in its DNA binding domain. Gel mobility shift assay demonstrated that GST-AtMYB305 fusion pro-tein bound specifically to the DNA fragment that included a consensus MYB recognition sequence (TAACTG). Overex-pression of AtMYB305 in the fission yeast caused the forma-tion of elongated cells with one condensed nucleus. Semi-quantitative RT-PCR analysis revealed that AtMYB305 was expressed specifically in the roots of Arabidopsis.
基金supported by the National Natural Science Foundation of China NSFC-U1809213National Natural Science Foundation of China NSFC-21975222.
文摘Spun-bond non-woven fabrics(NWFs)made of porous C-shaped polypropylene fibers were applied in rapid oil absorption and effective on-line oil spillage monitoring.It is of great interest to further optimize the absorption properties of these materials by tuning their preparation parameters as well as characterize them with theoretical models.In this paper,effects of die shape,diluent composition(mixtures of dibutyl and dioctyl phthalate),and drawing speed on their porous structure and oil-absorbing performance were systematically investigated and characterized based on two novel concepts,i.e.,the equivalent capillary tube pore radius and the kinetic pore tortuosity(barrier to access)derived from the simplest capillary tube liquid-filling model.The use of higher dibutyl phthalate fractions under faster drawing speeds resulted in the formation of larger and more connected inner filament sub-micron pores.Three stages of tube filling relating to inter-filament large pores,medium pores close to bonding points,and inner filament small pores were observed in the spun-bond NWFs.Continuous oil recovery rates of 986 L·m^(-2)·h^(-1) with an oil/water selectivity of 6.4 were achieved in dynamic skimming experiments using simulated spilled oil.
