Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide...Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide and C.crustorum LBK-CCMXJ1001(LBK1001)in Caenorhabditis elegans.Our findings revealed that fraction 1 of C.deserticola polysaccharide(CD-1)enhanced the proliferation and antioxidant properties of LBK1001 in vitro.LBK1001 significantly increased the number of head swinging,frequency of pharyngeal pumps,and the activities of antioxidant enzymes,including SOD,CAT,and GSH-px,and reduced the levels of oxidized free radicals,lipofuscin,and MDA in C.elegans.Furthermore,the LBK1001-CD-1 complex exhibited greater bioactive effects than LBK1001 alone.Both LBK1001 and the LBK1001-CD-1 complex decreased the expression of the daf-2 and akt-1 genes while increasing sod-3,daf-16,hsp-16.2,and skn-1 in C.elegans using qPCR analysis.Correlation analysis indicated that the expressions of daf-2 and akt-1 were negatively correlated with SOD,GSH-px,and CAT,but positively correlated with MDA,ROS,and lipofuscin.Conversely,daf-16,hsp-16.2,and skn-1 exhibited an inverse relationship with daf-2 and akt-1.This was closely associated with the reversal of aging symptoms in C.elegans.The LBK1001-CD-1 complex demonstrated enhanced effects on lifespan extension and antioxidant capacity in C.elegans compared to LBK1001 alone,and it prolonged lifespan by regulating the anti-oxidation system and the expressions of genes related to aging.This indicated that the LBK1001-CD-1 complex modulated the antioxidant defense system of C.elegans while scavenging peroxides,promoting motility,and delaying aging.展开更多
To investigate the influence of mixed fermentation of L.paracasei SMN-LBK and L.reuteri LBK on the formation of taste of kazak cheese,this study analysis the enzyme activities and non-volatile taste components metabol...To investigate the influence of mixed fermentation of L.paracasei SMN-LBK and L.reuteri LBK on the formation of taste of kazak cheese,this study analysis the enzyme activities and non-volatile taste components metabolites.Utilizing untargeted metabolomics,it has been discovered that mixed fermentation could regulate the biosynthesis of 24 pivotal taste metabolites required for the production of Kazak cheese by adjusting the tricarboxylic acid cycle(TCA cycle),phenylalanine metabolism,galactose metabolism,and c5-branched dibasic acid metabolism.These metabolites predominantly consist of 10 types of sour substances,6 types of kokumi substances,6 types of sweet substances,and 2 types of bitter substances.Consequently,it can significantly elevate the acidity and umami of cheese,while prolonging the duration of its taste.Furthermore,it has been observed that mixed fermentation enhances enzymatic activity,thereby improving the color and texture of cheese,and subsequently exerting a notable positive influence on its overall taste.This study provided insights into the mechanism of taste formation during mixed fermentation of Kazak cheese.展开更多
Cadmium(Cd)is an environmental non-biodegradable pollutant that induces toxic effects in humans.Therefore,there is a pressing need to identify new methods to relieve cadmium-induced toxic damage.In this study,Weizmann...Cadmium(Cd)is an environmental non-biodegradable pollutant that induces toxic effects in humans.Therefore,there is a pressing need to identify new methods to relieve cadmium-induced toxic damage.In this study,Weizmannia coagulans(formerly termed Bacillus coagulans)LBK,which was isolated from silage feed,exhibited robust Cd tolerance and adsorption capabilities.In vitro experiments demonstrated that its scavenging rate for 1,1-diphenyl-2-picryl-hydrazyl radical(DPPH)and hydroxyl radicals was 40% and 39%,respectively.In vivo,LBK significantly reduced the mortality rate of cadmium-exposed mice.Moreover,LBK increased the hepatic levels of superoxide dismutase(SOD)and catalase(CAT),and histopathology examination suggested that LBK could attenuate liver damage.W.coagulans LBK significantly altered the composition of the intestinal microbiota and increased the abundance of beneficial bacteria such as Leptospirillaceae and Lactobacillus.Metabolomics analysis of cecal contents revealed that LBK regulated amino acid metabolic disorders caused by Cd exposure and restored the levels of glutamic acid,leucine,and aspartic acid.Based on the aforementioned advantages,W.coagulans LBK may be considered a promising candidate for alleviating oxidative stress caused by acute Cd exposure.展开更多
Microbial fermentation is an important method to obtain antioxidant peptides.In this study,Lactobacillus par-acasei SMN-LBK was used to ferment biprotein(walnut milk)to enhance the antioxidant capacity of the active p...Microbial fermentation is an important method to obtain antioxidant peptides.In this study,Lactobacillus par-acasei SMN-LBK was used to ferment biprotein(walnut milk)to enhance the antioxidant capacity of the active peptide.The optimal fermentation process of 5%inoculum,1:7 plant protein ratio,37℃and 12 h resulted in 77.63%±0.36%DPPH scavenging,93.31%±0.25%ABTS scavenging and 79.37%±0.02%metal ion chelating capacity.The hydrolysis products were classified into three molecular weight classes using an ultra-filtration membrane system.DPAP-3 with a molecular weight of less than 3 kDa had the highest antioxidant activity.DPAP-3 was further separated into three subclasses using gel filtration chromatography.These sub-classes were characterized using nano-liquid chromatography-tandem mass spectrometry(NanoLC-MS/MS)and screened by searching https://biochemia.uwm.edu.Four antioxidant peptides were identified using a combi-nation of hydrophobic amino acids,positive ion-charge interactions,and numerical values.Two of these peptides were from animals and one from plants.Their amino acid sequences are as follows PCRGVLLR,IHIPLPRWV,QSKVLPVPQK,and KVLPVPQKA,with molecular docking binding energies of7.8,8.3,7.4,and7.7 kcal/mol,respectively.The results suggest that fermentation of plant-animal proteins with Lactobacillus paracasei can produce antioxidant peptides that can be used as functional ingredients in food products.This has the potential to conserve animal protein resources.展开更多
Resistant starch(RS)is a type of dietary fiber with prebiotic-like properties that can reduce fat accumulation,modulate glucose and insulin levels,and protect the gut barrier.These functions from RS are closely associ...Resistant starch(RS)is a type of dietary fiber with prebiotic-like properties that can reduce fat accumulation,modulate glucose and insulin levels,and protect the gut barrier.These functions from RS are closely associated with gut microbiota.RS was consumed to different extents by gut microbiota,but there are significant differences in the utilization rates.We summarized the interaction between each type of RS and gut microbiota and the role of RS in regulating gut microbiota and metabolic activities were reviewed.It focused on exploring the differences in the utilization of RS due to variations in glycoside hydrolases encoded by gut microbes,and the effects of differences in utilization on gut microbial structure,composition,and metabolism including short-chain fatty acid,bile acids,and tryptophan.Furthermore,taking mineral levels,hyperlipidemia,gastric injury,colon cancer,metabolic syndrome,and non-alcoholic fatty liver disease as examples,we elaborated on the role of RS in-teractions with intestinal flora in regulating human health.Finally,we discussed the application of artificial intelligence algorithms in RS modulating human health.The structure and composition of gut flora are signif-icantly heterogeneous in individuals,it is possible to combine in vitro and in vivo fermentation experiments with artificial intelligence algorithms such as machine learning algorithms to analyze the differences in the response of the gut flora and metabolic network to different RS.Based on differences,personalized guidance on diet,nutrition,and lifestyle can be provided based on individual characteristics to more effectively utilize RS for disease prevention and intervention.展开更多
基金supported by the Talent Program“Tianchi Talent(Young Doctor)”in Xinjiang Uygur Autonomous Regionthe Corps Guiding Science and Technology program projects(grant number 2023ZD087)+2 种基金the National Natural Science Foundation of China(grant number 32060548)Key Project of Karamay City in Xinjiang(2023kqzdyf0026)the Xinjiang Uygur Autonomous Region Regional Collaborative Innovation Program(grant number 2022E01028).
文摘Companilactobacillus crustorum,a bacterium that contributes to the flavor characteristics of Koumiss,has the potential to improve health.Here,we evaluated the anti-aging effects of Cistanche deserticola polysaccharide and C.crustorum LBK-CCMXJ1001(LBK1001)in Caenorhabditis elegans.Our findings revealed that fraction 1 of C.deserticola polysaccharide(CD-1)enhanced the proliferation and antioxidant properties of LBK1001 in vitro.LBK1001 significantly increased the number of head swinging,frequency of pharyngeal pumps,and the activities of antioxidant enzymes,including SOD,CAT,and GSH-px,and reduced the levels of oxidized free radicals,lipofuscin,and MDA in C.elegans.Furthermore,the LBK1001-CD-1 complex exhibited greater bioactive effects than LBK1001 alone.Both LBK1001 and the LBK1001-CD-1 complex decreased the expression of the daf-2 and akt-1 genes while increasing sod-3,daf-16,hsp-16.2,and skn-1 in C.elegans using qPCR analysis.Correlation analysis indicated that the expressions of daf-2 and akt-1 were negatively correlated with SOD,GSH-px,and CAT,but positively correlated with MDA,ROS,and lipofuscin.Conversely,daf-16,hsp-16.2,and skn-1 exhibited an inverse relationship with daf-2 and akt-1.This was closely associated with the reversal of aging symptoms in C.elegans.The LBK1001-CD-1 complex demonstrated enhanced effects on lifespan extension and antioxidant capacity in C.elegans compared to LBK1001 alone,and it prolonged lifespan by regulating the anti-oxidation system and the expressions of genes related to aging.This indicated that the LBK1001-CD-1 complex modulated the antioxidant defense system of C.elegans while scavenging peroxides,promoting motility,and delaying aging.
基金supported by the Corps Guiding Science and Technology Programme(2023ZD087)The regional projects of the National Natural Fund of China(32060548,32260594)+1 种基金Regional Collaborative Innovation Special Project of Xinjiang Uygur Autonomous Region(2022E01028)Tianchi Young Doctoral Programme of Excellence,Shihezi University High-level Talent Initiation Programme(2022ZK012).
文摘To investigate the influence of mixed fermentation of L.paracasei SMN-LBK and L.reuteri LBK on the formation of taste of kazak cheese,this study analysis the enzyme activities and non-volatile taste components metabolites.Utilizing untargeted metabolomics,it has been discovered that mixed fermentation could regulate the biosynthesis of 24 pivotal taste metabolites required for the production of Kazak cheese by adjusting the tricarboxylic acid cycle(TCA cycle),phenylalanine metabolism,galactose metabolism,and c5-branched dibasic acid metabolism.These metabolites predominantly consist of 10 types of sour substances,6 types of kokumi substances,6 types of sweet substances,and 2 types of bitter substances.Consequently,it can significantly elevate the acidity and umami of cheese,while prolonging the duration of its taste.Furthermore,it has been observed that mixed fermentation enhances enzymatic activity,thereby improving the color and texture of cheese,and subsequently exerting a notable positive influence on its overall taste.This study provided insights into the mechanism of taste formation during mixed fermentation of Kazak cheese.
基金supported by the National Natural Science Foundation of China(No.32260594),Chinathe Research Initiation Program for High-level Talents at Shihezi University(No.2022ZK012),China+1 种基金the Corps Guiding Science and Technology Plan Project(No.2023ZD087),Chinathe Tianchi Young Talented Doctor Project,China.
文摘Cadmium(Cd)is an environmental non-biodegradable pollutant that induces toxic effects in humans.Therefore,there is a pressing need to identify new methods to relieve cadmium-induced toxic damage.In this study,Weizmannia coagulans(formerly termed Bacillus coagulans)LBK,which was isolated from silage feed,exhibited robust Cd tolerance and adsorption capabilities.In vitro experiments demonstrated that its scavenging rate for 1,1-diphenyl-2-picryl-hydrazyl radical(DPPH)and hydroxyl radicals was 40% and 39%,respectively.In vivo,LBK significantly reduced the mortality rate of cadmium-exposed mice.Moreover,LBK increased the hepatic levels of superoxide dismutase(SOD)and catalase(CAT),and histopathology examination suggested that LBK could attenuate liver damage.W.coagulans LBK significantly altered the composition of the intestinal microbiota and increased the abundance of beneficial bacteria such as Leptospirillaceae and Lactobacillus.Metabolomics analysis of cecal contents revealed that LBK regulated amino acid metabolic disorders caused by Cd exposure and restored the levels of glutamic acid,leucine,and aspartic acid.Based on the aforementioned advantages,W.coagulans LBK may be considered a promising candidate for alleviating oxidative stress caused by acute Cd exposure.
基金supported by the regional projects of the National Natural Fund of China(32060548,32260594)the Science and Tech-nology Innovation Talent Programme of Xinjiang Production and Construction Corps(2023CB008-04)+1 种基金the Regional Collaborative Innovation Special Project of Xinjiang Uygur Autonomous Region(2022E01028)the Guiding Science and Technology Programme of the Corps(2023ZD087).
文摘Microbial fermentation is an important method to obtain antioxidant peptides.In this study,Lactobacillus par-acasei SMN-LBK was used to ferment biprotein(walnut milk)to enhance the antioxidant capacity of the active peptide.The optimal fermentation process of 5%inoculum,1:7 plant protein ratio,37℃and 12 h resulted in 77.63%±0.36%DPPH scavenging,93.31%±0.25%ABTS scavenging and 79.37%±0.02%metal ion chelating capacity.The hydrolysis products were classified into three molecular weight classes using an ultra-filtration membrane system.DPAP-3 with a molecular weight of less than 3 kDa had the highest antioxidant activity.DPAP-3 was further separated into three subclasses using gel filtration chromatography.These sub-classes were characterized using nano-liquid chromatography-tandem mass spectrometry(NanoLC-MS/MS)and screened by searching https://biochemia.uwm.edu.Four antioxidant peptides were identified using a combi-nation of hydrophobic amino acids,positive ion-charge interactions,and numerical values.Two of these peptides were from animals and one from plants.Their amino acid sequences are as follows PCRGVLLR,IHIPLPRWV,QSKVLPVPQK,and KVLPVPQKA,with molecular docking binding energies of7.8,8.3,7.4,and7.7 kcal/mol,respectively.The results suggest that fermentation of plant-animal proteins with Lactobacillus paracasei can produce antioxidant peptides that can be used as functional ingredients in food products.This has the potential to conserve animal protein resources.
基金supported by the Jiangsu Province Natural Science Foundation(Grant No.BK20221078)the National Natural Science Foundation of China(Grant No.32360584)+1 种基金the Research Initiation Program for High-level Talents at Shihezi University(Grant No.RCZK202356)Talent Program"Tianchi Talent(Young Doctor)"in Xinjiang Uygur Autonomous Region.
文摘Resistant starch(RS)is a type of dietary fiber with prebiotic-like properties that can reduce fat accumulation,modulate glucose and insulin levels,and protect the gut barrier.These functions from RS are closely associated with gut microbiota.RS was consumed to different extents by gut microbiota,but there are significant differences in the utilization rates.We summarized the interaction between each type of RS and gut microbiota and the role of RS in regulating gut microbiota and metabolic activities were reviewed.It focused on exploring the differences in the utilization of RS due to variations in glycoside hydrolases encoded by gut microbes,and the effects of differences in utilization on gut microbial structure,composition,and metabolism including short-chain fatty acid,bile acids,and tryptophan.Furthermore,taking mineral levels,hyperlipidemia,gastric injury,colon cancer,metabolic syndrome,and non-alcoholic fatty liver disease as examples,we elaborated on the role of RS in-teractions with intestinal flora in regulating human health.Finally,we discussed the application of artificial intelligence algorithms in RS modulating human health.The structure and composition of gut flora are signif-icantly heterogeneous in individuals,it is possible to combine in vitro and in vivo fermentation experiments with artificial intelligence algorithms such as machine learning algorithms to analyze the differences in the response of the gut flora and metabolic network to different RS.Based on differences,personalized guidance on diet,nutrition,and lifestyle can be provided based on individual characteristics to more effectively utilize RS for disease prevention and intervention.
