The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the pr...The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the prostate （TmLRP）. TmLRP and partial bladder neck mucosa were performed in 15 healthy adult male crossbred canines. Wound specimens were harvested at 3 days, and 1, 2, 3, and 4 weeks after operation, respectively. The histopathologic characteristics were observed by hematoxylin and eosin staining. The expression of cytokeratin 14 （CK14）, CK5, CK18, synaptophysin （Syn）, chromogranin A （CgA）, uroplakin, transforming growth factor-β1 （TGF-β1）, and TGF-β type Ⅱ receptor in prostatic urethra wound were examined by immunohistochemistry and real-time polymerase chain reaction, respectively. Van Gieson staining was performed to determine the expression of collagen fibers in prostatic urethra and bladder neck would. The results showed that the re-epithelialization of the prostatic urethra resulted from the mobilization of proliferating epithelial cells from residual prostate tissue under the wound. The proliferating cells expressed CK14, CK5, but not CK18, Syn, and CgA and re-epithelialize expressed uroplakin since 3 weeks. There were enhanced TGF-β1 and TGF-β type Ⅱ receptor expression in proliferating cells and regenerated cells, which correlated with specific phases of re-epithelialization. Compared with the re-epithelialization of the bladder neck, re-epithelialization of canine prostatic urethra was faster, and the expression of collagen fibers was relatively low. In conclusion, re-epithelialization in canine prostatic urethra resulted from prostate basal cells after TmLRP and this re-epithelialization way may represent the ideal healing method from anatomic repair to functional recovery after injury.展开更多
The purpose of the current ex vivo study was to compare the speed of vaporesection of human prostatic tissue with benign prostatic hyperplasia （BPH） and the depth of tissue damage using 70- and 120-W 2-tim laser dev...The purpose of the current ex vivo study was to compare the speed of vaporesection of human prostatic tissue with benign prostatic hyperplasia （BPH） and the depth of tissue damage using 70- and 120-W 2-tim laser devices. Fresh prostatic tissue specimens were obtained from five patients by open prostatectomy, and were divided into separate groups （70 and 120 W） based on the energy of the laser output （70 and 120 W, respectively）. The vaporesection speed, coagulation zone depth and the necrotic tissue layer in the prostatic tissue were evaluated. The current result showed that the speeds （mean±s.d.） of vaporesection were 5.21±0.66 and 10.39±1.15 g/5 min for the 70 and 120 W groups, respectively （P=0.000）. There was no difference in the depth of necrosis/ coagulation （0.98±0.1310.30±0.09 and 0.99±0.12/0.31±0.08 mm） for the 70 and 120 W groups, respectively. In conclusion, both 70- and 120-W 2μm laser devices had superficial tissue damage during the vaporesection of human prostate tissue; moreover, the 120-W laser offers a higher vaporesection speed than the 70-W laser.展开更多
We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characte...We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characteristics suggestive of benign prostate hyperplasia(BPH).A total of 468 consecutive patients with a detailed medical history were identified.All patients were evaluated by scoring subjective symptoms with the International Prostate Symptom Score(IPSS)and quality of life(QoL).The prostatic anatomical parameters were measured using transrectal ultrasonography,and postvoid residual urine and maximum flow rate(Q_(max))values were also determined.Pearsonfs correlation analysis revealed that both total prostate volume(TPV;r=0.160,P<0.001)and transitional zone volume(TZV;r=0.104,P=0.016)increased with patients7 age;however,no correlations were observed of TPV,TZV,transitional zone index(TZI),and transitional zone thickness(TZT)with IPSS or QoL(all P>0.05).Peripheral to transitional zone index(PTI)was found negatively correlated with total IPSS(r=-0.113,P=0.024),storage IPSS(r=-0.103,P=0.041),and voiding IPSS(r=-0.123,P-0.014).As regards the uroflowmetry characteristics,PTI(r=0.157,P=0.007)was indicated to be positively correlated with Q_(max)and negatively correlated with TZI(r=-0.119,P=0.042)and TZT(r=-0.118,P=0.045),but not correlated with TPV,TZV,or peripheral zone thickness(PZT)(all P>0.05).Postvoid residual urine(PVR)had not correlated with all the prostatic anatomical variables(all P>0.05).This is the first study that formally proposed the concept of PTI,which is an easy-to-measure prostate anatomical parameter which significantly correlates with total IPSS,storage IPSS,voiding IPSS,and Q_(max),suggesting that PTI would be useful in evaluating and managing men with lower urinary tract symptoms(LUTS)/BPH.However,well-designed studies are mandatory to verify the clinical utility of PTI.展开更多
Dear Editor, Benign prostatic hyperplasia （BPH） is the most common genitourinary tract disease in elderly males.The two-micron laser combines the advantages of efficient resection and rapid vaporization, resulting i...Dear Editor, Benign prostatic hyperplasia （BPH） is the most common genitourinary tract disease in elderly males.The two-micron laser combines the advantages of efficient resection and rapid vaporization, resulting in high-performance resection and excellent hemostasis in resection of the prostate adenoma, which makes it an effective and promising modality in BPH treatment. However, severe edema and necrosis of the remaining prostatic tissue after vaporesection of the prostatic adenoma may cause severe irritative and/or obstructive symptoms and may even result in the need for a second operation. Rapid wound re-epithelialization in the prostatic urethra can, however, reduce the incidence of postoperative complications after transurethral resection of the prostate （TURP）.展开更多
文摘The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the prostate （TmLRP）. TmLRP and partial bladder neck mucosa were performed in 15 healthy adult male crossbred canines. Wound specimens were harvested at 3 days, and 1, 2, 3, and 4 weeks after operation, respectively. The histopathologic characteristics were observed by hematoxylin and eosin staining. The expression of cytokeratin 14 （CK14）, CK5, CK18, synaptophysin （Syn）, chromogranin A （CgA）, uroplakin, transforming growth factor-β1 （TGF-β1）, and TGF-β type Ⅱ receptor in prostatic urethra wound were examined by immunohistochemistry and real-time polymerase chain reaction, respectively. Van Gieson staining was performed to determine the expression of collagen fibers in prostatic urethra and bladder neck would. The results showed that the re-epithelialization of the prostatic urethra resulted from the mobilization of proliferating epithelial cells from residual prostate tissue under the wound. The proliferating cells expressed CK14, CK5, but not CK18, Syn, and CgA and re-epithelialize expressed uroplakin since 3 weeks. There were enhanced TGF-β1 and TGF-β type Ⅱ receptor expression in proliferating cells and regenerated cells, which correlated with specific phases of re-epithelialization. Compared with the re-epithelialization of the bladder neck, re-epithelialization of canine prostatic urethra was faster, and the expression of collagen fibers was relatively low. In conclusion, re-epithelialization in canine prostatic urethra resulted from prostate basal cells after TmLRP and this re-epithelialization way may represent the ideal healing method from anatomic repair to functional recovery after injury.
文摘The purpose of the current ex vivo study was to compare the speed of vaporesection of human prostatic tissue with benign prostatic hyperplasia （BPH） and the depth of tissue damage using 70- and 120-W 2-tim laser devices. Fresh prostatic tissue specimens were obtained from five patients by open prostatectomy, and were divided into separate groups （70 and 120 W） based on the energy of the laser output （70 and 120 W, respectively）. The vaporesection speed, coagulation zone depth and the necrotic tissue layer in the prostatic tissue were evaluated. The current result showed that the speeds （mean±s.d.） of vaporesection were 5.21±0.66 and 10.39±1.15 g/5 min for the 70 and 120 W groups, respectively （P=0.000）. There was no difference in the depth of necrosis/ coagulation （0.98±0.1310.30±0.09 and 0.99±0.12/0.31±0.08 mm） for the 70 and 120 W groups, respectively. In conclusion, both 70- and 120-W 2μm laser devices had superficial tissue damage during the vaporesection of human prostate tissue; moreover, the 120-W laser offers a higher vaporesection speed than the 70-W laser.
基金This study was supported by grants from the research program of National Science Foundation of China(No.81860141)Health and Family Planning Commission of Guizhou Province Foundation(No.gzwjkj2017-1-032)Doctoral Foundation of Guizhou Provincial Peoples Hospital(No.GZSYBS[2016]11).
文摘We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characteristics suggestive of benign prostate hyperplasia(BPH).A total of 468 consecutive patients with a detailed medical history were identified.All patients were evaluated by scoring subjective symptoms with the International Prostate Symptom Score(IPSS)and quality of life(QoL).The prostatic anatomical parameters were measured using transrectal ultrasonography,and postvoid residual urine and maximum flow rate(Q_(max))values were also determined.Pearsonfs correlation analysis revealed that both total prostate volume(TPV;r=0.160,P<0.001)and transitional zone volume(TZV;r=0.104,P=0.016)increased with patients7 age;however,no correlations were observed of TPV,TZV,transitional zone index(TZI),and transitional zone thickness(TZT)with IPSS or QoL(all P>0.05).Peripheral to transitional zone index(PTI)was found negatively correlated with total IPSS(r=-0.113,P=0.024),storage IPSS(r=-0.103,P=0.041),and voiding IPSS(r=-0.123,P-0.014).As regards the uroflowmetry characteristics,PTI(r=0.157,P=0.007)was indicated to be positively correlated with Q_(max)and negatively correlated with TZI(r=-0.119,P=0.042)and TZT(r=-0.118,P=0.045),but not correlated with TPV,TZV,or peripheral zone thickness(PZT)(all P>0.05).Postvoid residual urine(PVR)had not correlated with all the prostatic anatomical variables(all P>0.05).This is the first study that formally proposed the concept of PTI,which is an easy-to-measure prostate anatomical parameter which significantly correlates with total IPSS,storage IPSS,voiding IPSS,and Q_(max),suggesting that PTI would be useful in evaluating and managing men with lower urinary tract symptoms(LUTS)/BPH.However,well-designed studies are mandatory to verify the clinical utility of PTI.
基金ACKNOWLEDGMENTS This study was supported by the Natural Science Foundation of China （81260119） and the Guizhou Science and Technology Agency Fund （[201214013 SZ201213077]+1 种基金 SY2011 [3024] LS2011 [020]）.
文摘Dear Editor, Benign prostatic hyperplasia （BPH） is the most common genitourinary tract disease in elderly males.The two-micron laser combines the advantages of efficient resection and rapid vaporization, resulting in high-performance resection and excellent hemostasis in resection of the prostate adenoma, which makes it an effective and promising modality in BPH treatment. However, severe edema and necrosis of the remaining prostatic tissue after vaporesection of the prostatic adenoma may cause severe irritative and/or obstructive symptoms and may even result in the need for a second operation. Rapid wound re-epithelialization in the prostatic urethra can, however, reduce the incidence of postoperative complications after transurethral resection of the prostate （TURP）.
