目的对社会工作介入慢病管理的国际研究热点及变化趋势进行可视化分析。方法检索2000-2024年Web of Science数据库中核心合集内社会工作介入慢病管理的研究,利用CiteSpace软件可视化分析。结果研究趋势整体呈现波动上升,出现频次位居前...目的对社会工作介入慢病管理的国际研究热点及变化趋势进行可视化分析。方法检索2000-2024年Web of Science数据库中核心合集内社会工作介入慢病管理的研究,利用CiteSpace软件可视化分析。结果研究趋势整体呈现波动上升,出现频次位居前五的关键词分别是社会支持(social support)、管理(management)、生活质量(quality of life)、慢病(chronic disease)、照护(care),存在多机构、多作者合作。结论国际上社会工作围绕社会支持与生活质量展开对慢病管理的介入,相关领域科研合作体系呈现多元化,社会工作者在慢病管理的跨学科团队中发挥积极作用。展开更多
为了评估华支睾吸虫(Clonorchis sinensis)细胞外膜囊泡中虫源miRNAs跨物种调控宿主基因的风险,试验利用miRBase数据库对华支睾吸虫细胞外膜囊泡和感染阳性宿主细胞共有的虫源miRNAs成熟体序列进行筛选,通过miRanda软件对细胞外膜囊泡...为了评估华支睾吸虫(Clonorchis sinensis)细胞外膜囊泡中虫源miRNAs跨物种调控宿主基因的风险,试验利用miRBase数据库对华支睾吸虫细胞外膜囊泡和感染阳性宿主细胞共有的虫源miRNAs成熟体序列进行筛选,通过miRanda软件对细胞外膜囊泡中表达量排在前10位的miRNAs进行靶基因预测,选取每个miRNAs评分排在前10位的靶基因作为候选靶基因,通过NCBI、Bing检索工具检索miRNAs候选靶基因的功能,利用GO功能注释及KEGG富集分析对靶基因进行生物信息学分析。结果表明:华支睾吸虫细胞外膜囊泡中的10种miRNAs共对应8128个与宿主相关的靶基因。有12个候选靶基因没有相关的功能报道,12个候选靶基因与肝功能有关,其余候选靶基因在癌症、信号转导、结构骨架、增殖和分化、迁移功能中发挥重要作用。候选靶基因Taf10和Onecut2与肝脏和肝胆管发育相关,候选靶基因Gng1和Hhip分别可参与致纤维化相关的丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)和Hedgehog信号通路。说明虫源miRNAs有靶向宿主细胞并参与致宿主肝纤维化和肝癌的风险。展开更多
OBJECTIVE: To investigate the in-depth pharmacological mechanisms of celastrol in children neuroblastoma treatment. METHODS: In the current study, we examined the effects of celastrol on children neuroblastoma cells v...OBJECTIVE: To investigate the in-depth pharmacological mechanisms of celastrol in children neuroblastoma treatment. METHODS: In the current study, we examined the effects of celastrol on children neuroblastoma cells viability and proliferation by cell counting kit-8 assay and colony formation assay. Annexin V-FTIC and PI staining were applied to determine cell apoptosis after celastrol treatment. ROS generation levels were examined by 2′, 7′-dichloroflfluorescin diacetate. RESULTS: We found that celastrol could suppress the proliferation of children neuroblastoma cells with few effects on normal cell lines in vitro. Further mechanisms studies have shown that celastrol inhibited cell cycle progression and induced cell apoptosis in QDDQ-NM and SH-SY5Y cells. In addition, ROS production might involve in celastrol-mediated apoptotic cell death in children neuroblastoma cells by activating caspase death pathway. CONCLUSIONS: Our findings demonstrated that celastrol could promote ROS generation-induced apoptosis in neuroblastoma cell by activating caspase death pathway. These findings suggested that celastrol might be a potential novel anti-neuroblastoma agent with minor cytotoxicity.展开更多
文摘目的对社会工作介入慢病管理的国际研究热点及变化趋势进行可视化分析。方法检索2000-2024年Web of Science数据库中核心合集内社会工作介入慢病管理的研究,利用CiteSpace软件可视化分析。结果研究趋势整体呈现波动上升,出现频次位居前五的关键词分别是社会支持(social support)、管理(management)、生活质量(quality of life)、慢病(chronic disease)、照护(care),存在多机构、多作者合作。结论国际上社会工作围绕社会支持与生活质量展开对慢病管理的介入,相关领域科研合作体系呈现多元化,社会工作者在慢病管理的跨学科团队中发挥积极作用。
文摘为了评估华支睾吸虫(Clonorchis sinensis)细胞外膜囊泡中虫源miRNAs跨物种调控宿主基因的风险,试验利用miRBase数据库对华支睾吸虫细胞外膜囊泡和感染阳性宿主细胞共有的虫源miRNAs成熟体序列进行筛选,通过miRanda软件对细胞外膜囊泡中表达量排在前10位的miRNAs进行靶基因预测,选取每个miRNAs评分排在前10位的靶基因作为候选靶基因,通过NCBI、Bing检索工具检索miRNAs候选靶基因的功能,利用GO功能注释及KEGG富集分析对靶基因进行生物信息学分析。结果表明:华支睾吸虫细胞外膜囊泡中的10种miRNAs共对应8128个与宿主相关的靶基因。有12个候选靶基因没有相关的功能报道,12个候选靶基因与肝功能有关,其余候选靶基因在癌症、信号转导、结构骨架、增殖和分化、迁移功能中发挥重要作用。候选靶基因Taf10和Onecut2与肝脏和肝胆管发育相关,候选靶基因Gng1和Hhip分别可参与致纤维化相关的丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)和Hedgehog信号通路。说明虫源miRNAs有靶向宿主细胞并参与致宿主肝纤维化和肝癌的风险。
基金Supported by the Scientific Research Project of Shaanxi Provincial Department of Health:the Protective Role of Celastrol and its Active Mechanisms in Neuroblastoma Treatment (2020A1515003129)。
文摘OBJECTIVE: To investigate the in-depth pharmacological mechanisms of celastrol in children neuroblastoma treatment. METHODS: In the current study, we examined the effects of celastrol on children neuroblastoma cells viability and proliferation by cell counting kit-8 assay and colony formation assay. Annexin V-FTIC and PI staining were applied to determine cell apoptosis after celastrol treatment. ROS generation levels were examined by 2′, 7′-dichloroflfluorescin diacetate. RESULTS: We found that celastrol could suppress the proliferation of children neuroblastoma cells with few effects on normal cell lines in vitro. Further mechanisms studies have shown that celastrol inhibited cell cycle progression and induced cell apoptosis in QDDQ-NM and SH-SY5Y cells. In addition, ROS production might involve in celastrol-mediated apoptotic cell death in children neuroblastoma cells by activating caspase death pathway. CONCLUSIONS: Our findings demonstrated that celastrol could promote ROS generation-induced apoptosis in neuroblastoma cell by activating caspase death pathway. These findings suggested that celastrol might be a potential novel anti-neuroblastoma agent with minor cytotoxicity.
