Objective: To evaluate the improvement effect of combined treatment of eschar abrasion, nanosilver dressing, and mussel mucin spray on wound healing in patients with second-degree burns, and to explore their effects o...Objective: To evaluate the improvement effect of combined treatment of eschar abrasion, nanosilver dressing, and mussel mucin spray on wound healing in patients with second-degree burns, and to explore their effects on the expression level of (advanced glycation end products) AGEs in wound tissue, so as to provide a basis for the application of AGEs expression level in wound tissue in the future clinical treatment of second-degree burns. Methods: Patients with second-degree burns admitted to the Department of Burns and Plastic Surgery of the Second Affiliated Hospital of Shantou University Medical College from July 2023 to July 2024 were selected as the research subjects. This study was a non-double-blind study, and both patients and researchers were aware of the treatment methods. They were randomly divided into Group A (control group) and Group B (study group). According to the order of their visits, the patients were numbered in advance;then the seed number was taken, and 70 random numbers were generated on the computer using SAS, the first 35 corresponding to group A, and the last 35 corresponding to group B;the random numbers were arranged from small to large, and the rank of the random numbers was the patient number;finally, the patient numbers were arranged from small to large, and the corresponding groups were the grouping scheme. 1) Wound healing time: The wound healing was observed every day, and the wound healing time was calculated when the wound was completely epithelialized. 2) Wound healing: The wound healing area was measured 1, 7, and 14 days after treatment, and transparent paper was used to record combined with a computer-assisted imaging system. Wound healing rate = wound healing area (cm2)/total wound area before treatment (cm2) × 100%. The time for complete wound healing was recorded in the two groups of patients. 3) Pain: The pain was evaluated at 1, 7, and 14 days after treatment using the visual analogue scale (VAS). The higher the score, the more severe the pain. 4) Scar condition: Scar formation was evaluated 1, 3, and 6 months after wound healing using the VAS scale, with a total score of 15 points. The higher the score, the more severe the scar. 5) Detect the expression of AGEs in wound tissue. Results: The wound healed 14.03 ± 2.28 days after eschar removal, with a cure rate of 97.8%. No infection occurred in the wound after eschar removal in all patients. The wound healing time ranged from 9 to 23 days, with an average healing time of 28.41 ± 1.45 days. The healing quality was satisfactory. The Vancouver Scar Scale scored the wound healing scar as 0.81 ± 0.73 points. Western blot was used to detect the expression of AGEs, (receptor for advanced glycation end products) RAGE, and protein in the wound tissue. Results After 7, 14, and 28 days of medication, the wounds of diabetic patients healed well. The drug treatment efficacy and hydroxyproline content showed an upward trend, and the expression levels of AGEs, RAGE, and HIF-1a proteins showed a downward trend. Compared with day 0, the hydroxyproline content of the wound granulation tissue on days 7, 14, and 28 was significantly increased (P< 0.01), and the expression levels of AGEs, RAGE, and HIF-1α proteins were significantly downregulated (P< 0.05 or P< 0.01). Conclusion: Monitoring the expression levels of AGEs and RAGE can reflect the wound-healing effect of patients with second-degree burns, and the prognosis of the wound is closely related to the expression levels of AGEs, RAGE, and scab abrasion. In the treatment of second-degree burn wounds, scab abrasion can grasp the level of necrotic scab removal, can achieve “relatively accurate” removal of necrotic tissue, maximally protect the ecological tissue between wounds and retaining normal tissue, and can play a positive role in promoting the healing process of burn wounds;at the same time, nanosilver dressings have good antibacterial properties and high safety, and mussel mucin has anti-inflammatory and antioxidant activities and the characteristics of blocking nerve endings, which can effectively relieve the burning, stinging, and itching of sensitive skin and effectively promote wound healing.展开更多
Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands a...Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands are equally innervated by the sympathetic nerve fibers. To welt understand the mechanisms on sweat secretion and reabsorption, the differential innervation of secretory coils and ducts in human eccrine sweat glands was investigated in the study. Methods： From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 μm-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 （PGP 9.5）, tyrosine hydroxylase （TH） and vasoactive intestinal peptide （VIP）, and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification （IF-TSA）. Stained sections were observed under the microscope, photographed, and analyzed. Results： The fluorescent signals of PGP 9.5, TH, and VIP were easily visualized, by 1F-TSA, as circular patterns surrounding eccrine sweat glands, but only PGP 9.5 could be observed by standard IF. The IF-TSA method is more sensitivity than standard IF in detecting antigens expressed at low levels. PGP 9.5, TH, and VIP appeared primarily surrounding the secretory coils and sparsely surrounding the sweat ducts. Conclusion： Sweat secretion is mainly controlled by autonomic nerves whereas sweat reabsorption is less affected by nerve activity.展开更多
文摘Objective: To evaluate the improvement effect of combined treatment of eschar abrasion, nanosilver dressing, and mussel mucin spray on wound healing in patients with second-degree burns, and to explore their effects on the expression level of (advanced glycation end products) AGEs in wound tissue, so as to provide a basis for the application of AGEs expression level in wound tissue in the future clinical treatment of second-degree burns. Methods: Patients with second-degree burns admitted to the Department of Burns and Plastic Surgery of the Second Affiliated Hospital of Shantou University Medical College from July 2023 to July 2024 were selected as the research subjects. This study was a non-double-blind study, and both patients and researchers were aware of the treatment methods. They were randomly divided into Group A (control group) and Group B (study group). According to the order of their visits, the patients were numbered in advance;then the seed number was taken, and 70 random numbers were generated on the computer using SAS, the first 35 corresponding to group A, and the last 35 corresponding to group B;the random numbers were arranged from small to large, and the rank of the random numbers was the patient number;finally, the patient numbers were arranged from small to large, and the corresponding groups were the grouping scheme. 1) Wound healing time: The wound healing was observed every day, and the wound healing time was calculated when the wound was completely epithelialized. 2) Wound healing: The wound healing area was measured 1, 7, and 14 days after treatment, and transparent paper was used to record combined with a computer-assisted imaging system. Wound healing rate = wound healing area (cm2)/total wound area before treatment (cm2) × 100%. The time for complete wound healing was recorded in the two groups of patients. 3) Pain: The pain was evaluated at 1, 7, and 14 days after treatment using the visual analogue scale (VAS). The higher the score, the more severe the pain. 4) Scar condition: Scar formation was evaluated 1, 3, and 6 months after wound healing using the VAS scale, with a total score of 15 points. The higher the score, the more severe the scar. 5) Detect the expression of AGEs in wound tissue. Results: The wound healed 14.03 ± 2.28 days after eschar removal, with a cure rate of 97.8%. No infection occurred in the wound after eschar removal in all patients. The wound healing time ranged from 9 to 23 days, with an average healing time of 28.41 ± 1.45 days. The healing quality was satisfactory. The Vancouver Scar Scale scored the wound healing scar as 0.81 ± 0.73 points. Western blot was used to detect the expression of AGEs, (receptor for advanced glycation end products) RAGE, and protein in the wound tissue. Results After 7, 14, and 28 days of medication, the wounds of diabetic patients healed well. The drug treatment efficacy and hydroxyproline content showed an upward trend, and the expression levels of AGEs, RAGE, and HIF-1a proteins showed a downward trend. Compared with day 0, the hydroxyproline content of the wound granulation tissue on days 7, 14, and 28 was significantly increased (P< 0.01), and the expression levels of AGEs, RAGE, and HIF-1α proteins were significantly downregulated (P< 0.05 or P< 0.01). Conclusion: Monitoring the expression levels of AGEs and RAGE can reflect the wound-healing effect of patients with second-degree burns, and the prognosis of the wound is closely related to the expression levels of AGEs, RAGE, and scab abrasion. In the treatment of second-degree burn wounds, scab abrasion can grasp the level of necrotic scab removal, can achieve “relatively accurate” removal of necrotic tissue, maximally protect the ecological tissue between wounds and retaining normal tissue, and can play a positive role in promoting the healing process of burn wounds;at the same time, nanosilver dressings have good antibacterial properties and high safety, and mussel mucin has anti-inflammatory and antioxidant activities and the characteristics of blocking nerve endings, which can effectively relieve the burning, stinging, and itching of sensitive skin and effectively promote wound healing.
基金This study was supported by grants from National Natural Science Foundation of China （No. 81772102, and No. 81471882）.
文摘Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands are equally innervated by the sympathetic nerve fibers. To welt understand the mechanisms on sweat secretion and reabsorption, the differential innervation of secretory coils and ducts in human eccrine sweat glands was investigated in the study. Methods： From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 μm-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 （PGP 9.5）, tyrosine hydroxylase （TH） and vasoactive intestinal peptide （VIP）, and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification （IF-TSA）. Stained sections were observed under the microscope, photographed, and analyzed. Results： The fluorescent signals of PGP 9.5, TH, and VIP were easily visualized, by 1F-TSA, as circular patterns surrounding eccrine sweat glands, but only PGP 9.5 could be observed by standard IF. The IF-TSA method is more sensitivity than standard IF in detecting antigens expressed at low levels. PGP 9.5, TH, and VIP appeared primarily surrounding the secretory coils and sparsely surrounding the sweat ducts. Conclusion： Sweat secretion is mainly controlled by autonomic nerves whereas sweat reabsorption is less affected by nerve activity.
