Autophagy plays a vital role in maintaining the balance of normal physiological state of living cells.In this paper,a polarity-specific two-phot on fluorescent probe Lyso-NA based on naphthalimide was synthesized for ...Autophagy plays a vital role in maintaining the balance of normal physiological state of living cells.In this paper,a polarity-specific two-phot on fluorescent probe Lyso-NA based on naphthalimide was synthesized for the purpose of monitoring autophagy during biological research.The results of photophysical properties and theoretical calculation con firmed that different polarities of solvents mainly effected fluorescent intensities of probe.Fluoresce nt intensity,quantum yield and fluorescence lifetime of probe kept a good linear relationship with polarity respectively.In addition,due to its low toxicity and selective accumulation in lysosomes,Lyso-NA is suitable for detecting changes in lysosomal polarity of living cells.Compare with the imaging results of plasmid transfection,a better performed realtime long-term fluoresce nt visualization of autophagy in living cells was achieved.Probe Lyso-NA can work as an efficient and cost effective imaging tool for visualizing autophagy in living cells.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.22077001,21778001 and 21672001)the Natural Science Foundation of Anhui Province(No.2008085J08)+2 种基金the Natural Science Foundation of Education Department of Anhui Province(No.KJ2019A0010)Joint Open Fund of Jiangsu Collaborative Innovation Center for Ecological Building Material and Environmental Protection Equipments,Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province(No.JH201803)the Open fund for Discipline Construction in Institute of Physical Science and In formation Technology of Anhui University.
文摘Autophagy plays a vital role in maintaining the balance of normal physiological state of living cells.In this paper,a polarity-specific two-phot on fluorescent probe Lyso-NA based on naphthalimide was synthesized for the purpose of monitoring autophagy during biological research.The results of photophysical properties and theoretical calculation con firmed that different polarities of solvents mainly effected fluorescent intensities of probe.Fluoresce nt intensity,quantum yield and fluorescence lifetime of probe kept a good linear relationship with polarity respectively.In addition,due to its low toxicity and selective accumulation in lysosomes,Lyso-NA is suitable for detecting changes in lysosomal polarity of living cells.Compare with the imaging results of plasmid transfection,a better performed realtime long-term fluoresce nt visualization of autophagy in living cells was achieved.Probe Lyso-NA can work as an efficient and cost effective imaging tool for visualizing autophagy in living cells.
