In the natural world,leaf-cutting ants cause vibrations through their mutual scraping of file-scraper organs.In this study,we designed a Biomimetic Ultrasonic Exciter(BUE)that imitates leaf-cutting ants.The operating ...In the natural world,leaf-cutting ants cause vibrations through their mutual scraping of file-scraper organs.In this study,we designed a Biomimetic Ultrasonic Exciter(BUE)that imitates leaf-cutting ants.The operating characteristics of the BUE were studied through experimental testing and finite element simulations.The results showed that the BUE could generate stable ultrasonic vibrations,and that the excitation frequency only needed to be half the Output Frequency(OF).This frequency-doubling phenomenon was conducive to achieving BUE miniaturization.To further explore the phenomenon of frequency-doubling vibration output,this study designed scrapers of five different sizes,conducted excitation and first-order natural frequency measurement tests,and the corresponding finite element simulations.It was found that each scraper could operate in frequency-doubling mode,but the operating frequency and natural mode frequencies did not correspond with one another.To further explicate experimental and simulation results,a two-degrees-of-freedom vibration model was developed.It was evident that the contact relationship between the dentate disc and scraper introduced strong nonlinear factors into the system,accounting for the frequency-doubling phenomenon and the difference between the BUE’s operating and mode frequencies.The BUE could be expected to facilitate the production of high-power micro-ultrasonic generators and has potential application value in the fields of mechanical processing,industrial production,and medical health.展开更多
Grass carp reovirus（GCRV）,a member of the genus Aquareovirus in the family Reoviridae（Attoui H,et al,2002）,is one of the most virulent aquareoviruses iden-tified so far,leading to high fatality rates（up to 85%）o...Grass carp reovirus（GCRV）,a member of the genus Aquareovirus in the family Reoviridae（Attoui H,et al,2002）,is one of the most virulent aquareoviruses iden-tified so far,leading to high fatality rates（up to 85%）of fingerlings and yearlings of grass carp（Fang Q,et al,2007）.No commercial vaccines against GCRV infection have been developed as yet.展开更多
Mitophagy maintains tissue homeostasis by self-eliminating defective mitochondria through autophagy.How mitophagy regulates stem cell activity during hair regeneration remains unclear.Here,we found that mitophagy prom...Mitophagy maintains tissue homeostasis by self-eliminating defective mitochondria through autophagy.How mitophagy regulates stem cell activity during hair regeneration remains unclear.Here,we found that mitophagy promotes the proliferation of hair germ(HG)cells by regulating glutathione(GSH)metabolism.First,single-cell RNA sequencing,mitochondrial probe,transmission electron microscopy,and immunofluorescence staining showed stronger mitochondrial activity and increased mitophagy-related gene especially Prohibitin 2(Phb2)expression at early-anagen HG compared to the telogen HG.Mitochondrial inner membrane receptor protein PHB2 binds to LC3 to initiate mitophagy.Second,molecular docking and functional studies revealed that PHB2-LC3 activates mitophagy to eliminate the damaged mitochondria in HG.RNA-seq,single-cell metabolism,immunofluorescence staining,and functional validation discovered that LC3 promotes GSH metabolism to supply energy for promoting HG proliferation.Third,transcriptomics analysis and immunofluorescence staining indicated that mitophagy was down-regulated in the aged compared to young-mouse HG.Activating mitophagy and GSH pathways through small-molecule administration can reactivate HG cell proliferation followed by hair regeneration in aged hair follicles.Our findings open up a new avenue for exploring autophagy that promotes hair regeneration and emphasizes the role of the self-elimination effect of mitophagy in controlling the proliferation of HG cells by regulating GSH metabolism.展开更多
The threat of environmental degradation attracts great attention to clean energy production and transportation.However,the limited scope of energy consumption causes the large-scale of clean energy sources to be aband...The threat of environmental degradation attracts great attention to clean energy production and transportation.However,the limited scope of energy consumption causes the large-scale of clean energy sources to be abandoned in Sichuan province.In the meantime,the development of modern greenhouse cultivation has transformed the agriculture industry to develop a brand-new type of electrical load in the grid.Consequently,the agricultural load can be used to consume the clean energy while facilitating plant growth.In this paper,an innovative agricultural load model is proposed based on crop evapotranspiration and daily light integration.Furthermore,the proposed agricultural load model is also applied to investigate the electricity consumption of five types of crop planting.The results illustrate that the power consumption is primarily driven by an artificial lighting compensation system.展开更多
基金supported by the National Natural Science Foundation of China(grant nos.91960203,51975035)。
文摘In the natural world,leaf-cutting ants cause vibrations through their mutual scraping of file-scraper organs.In this study,we designed a Biomimetic Ultrasonic Exciter(BUE)that imitates leaf-cutting ants.The operating characteristics of the BUE were studied through experimental testing and finite element simulations.The results showed that the BUE could generate stable ultrasonic vibrations,and that the excitation frequency only needed to be half the Output Frequency(OF).This frequency-doubling phenomenon was conducive to achieving BUE miniaturization.To further explore the phenomenon of frequency-doubling vibration output,this study designed scrapers of five different sizes,conducted excitation and first-order natural frequency measurement tests,and the corresponding finite element simulations.It was found that each scraper could operate in frequency-doubling mode,but the operating frequency and natural mode frequencies did not correspond with one another.To further explicate experimental and simulation results,a two-degrees-of-freedom vibration model was developed.It was evident that the contact relationship between the dentate disc and scraper introduced strong nonlinear factors into the system,accounting for the frequency-doubling phenomenon and the difference between the BUE’s operating and mode frequencies.The BUE could be expected to facilitate the production of high-power micro-ultrasonic generators and has potential application value in the fields of mechanical processing,industrial production,and medical health.
基金supported by the Natural Science Foundation of China(30901118,31172433)National Science & Technology Pillar Program(2012BAD25B06)Fundamental Research Funds for the Central Universities(2011PY121,2011QC029,2013PY069,2013PY70,2013PY071)
文摘Grass carp reovirus（GCRV）,a member of the genus Aquareovirus in the family Reoviridae（Attoui H,et al,2002）,is one of the most virulent aquareoviruses iden-tified so far,leading to high fatality rates（up to 85%）of fingerlings and yearlings of grass carp（Fang Q,et al,2007）.No commercial vaccines against GCRV infection have been developed as yet.
基金supported by the National Key Research and Development Program of China(2023YFC2508200)the National Natural Science Foundation of China(82373509,82003384,and 82203960)+5 种基金Fundamental Research Funds for the Central Universities(2022CDJYGRH-003 and 2023CDJKYJH066)the Natural Science Foundation of Chongqing(CSTB2023JXJL-YFX0028)Chongqing Talents:Exceptional Young Talents Project(cstc2021ycjh-bgzxm0197)Inheritance and Innovation Team of TCM Treatment of Immune Diseases,Scientific Research Foundation from Chongqing University(02210011044110)Research Horizontal Projects of Chongqing University(H20231432 and H20230936),ChinaC.-M.C.is supported by a research contract(GR1035751)between USC and CMU and US NIH grant R37 AR060306.
文摘Mitophagy maintains tissue homeostasis by self-eliminating defective mitochondria through autophagy.How mitophagy regulates stem cell activity during hair regeneration remains unclear.Here,we found that mitophagy promotes the proliferation of hair germ(HG)cells by regulating glutathione(GSH)metabolism.First,single-cell RNA sequencing,mitochondrial probe,transmission electron microscopy,and immunofluorescence staining showed stronger mitochondrial activity and increased mitophagy-related gene especially Prohibitin 2(Phb2)expression at early-anagen HG compared to the telogen HG.Mitochondrial inner membrane receptor protein PHB2 binds to LC3 to initiate mitophagy.Second,molecular docking and functional studies revealed that PHB2-LC3 activates mitophagy to eliminate the damaged mitochondria in HG.RNA-seq,single-cell metabolism,immunofluorescence staining,and functional validation discovered that LC3 promotes GSH metabolism to supply energy for promoting HG proliferation.Third,transcriptomics analysis and immunofluorescence staining indicated that mitophagy was down-regulated in the aged compared to young-mouse HG.Activating mitophagy and GSH pathways through small-molecule administration can reactivate HG cell proliferation followed by hair regeneration in aged hair follicles.Our findings open up a new avenue for exploring autophagy that promotes hair regeneration and emphasizes the role of the self-elimination effect of mitophagy in controlling the proliferation of HG cells by regulating GSH metabolism.
基金supported by the Talents'Training Quality and Teaching Reform Project for 2018-2020 Higher Education in Sichuan Province(JG2018-10)the New Century Higher Education Teaching Reform Project of Sichuan University under Grant No.SCU8007.
文摘The threat of environmental degradation attracts great attention to clean energy production and transportation.However,the limited scope of energy consumption causes the large-scale of clean energy sources to be abandoned in Sichuan province.In the meantime,the development of modern greenhouse cultivation has transformed the agriculture industry to develop a brand-new type of electrical load in the grid.Consequently,the agricultural load can be used to consume the clean energy while facilitating plant growth.In this paper,an innovative agricultural load model is proposed based on crop evapotranspiration and daily light integration.Furthermore,the proposed agricultural load model is also applied to investigate the electricity consumption of five types of crop planting.The results illustrate that the power consumption is primarily driven by an artificial lighting compensation system.
