As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobi...As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor(A^(2)OA-MBR)should be emphasized.In this study,systematic optimization of coagulation parameters for greater pollutant removal was conducted in terms of coagulant dosage,coagulation residence time and stirring hydraulic conditions.Coagulation process could remarkably remove turbidity,phosphorus,chemical oxygen demand,humic-like,protein-like and polysaccharide-like substances from secondary effluent and A^(2)OA sludge-liquid mixture.Furthermore,the influence of coagulation on membrane fouling development during the ultrafiltration of secondary effluent and A^(2)OA sludge-liquid mixture was investigated based on optimum coagulation parameters.Coagulation simultaneously reduced reversible membrane fouling and the irreversible one by 86%and 16%,respectively.According to excitation-emission matrix and attenuated total reflection-fourier transform infrared spectra,membrane fouling was primarily influenced by the cake layer,although pore fouling might be aggravated by A^(2)OA processes.Besides,the feasibility of coagulation-assisted A^(2)OA-MBRwas also assessed using hollowfibermembranes.It exhibited excellent potential in alleviatingmembrane fouling,while regular cleaning twice a day was not enough to suppress transmembrane pressure increase during direct domestic wastewater ultrafiltration.Additionally,both polysaccharide-like and protein-like foulants were vital components for membrane fouling during wastewater treatment.展开更多
Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reducti...Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.展开更多
Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potent...Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.展开更多
The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection ...The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection in the field of blasting.Serverless Computing can provide a variety of computing services for people without hardware foundations and rich software development experience,which has aroused people’s interest in how to use it in the field ofmachine learning.In this paper,we design a distributedmachine learning training application based on the AWS Lambda platform.Based on data parallelism,the data aggregation and training synchronization in Function as a Service(FaaS)are effectively realized.It also encrypts the data set,effectively reducing the risk of data leakage.We rent a cloud server and a Lambda,and then we conduct experiments to evaluate our applications.Our results indicate the effectiveness,rapidity,and economy of distributed training on FaaS.展开更多
At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degr...At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.展开更多
Objective:Preoperative prediction of microvascular invasion(MVI)in patients with hepatocellular cancer(HCC)is important for developing surgical strategy.We aimed to develop and validate a combined intratumoral and per...Objective:Preoperative prediction of microvascular invasion(MVI)in patients with hepatocellular cancer(HCC)is important for developing surgical strategy.We aimed to develop and validate a combined intratumoral and peritumoral radiomics model based on gadolinium-ethoxybenzyl-diethylenetriaminepentaacetic acid(Gd-EOB-DTPA)-enhanced magnetic resonance imaging(MRI)for preoperative prediction of MVI in primary HCC patients.展开更多
To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and othe...To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and other retrieval tools,the statistics and analysis of 3152 research literatures on sponge city published in domestic academic journals of 2004-2016 are conducted based on bibliometrics. It is found that since the concept of " sponge city" was firstly proposed in 2012,development research of sponge city involves 40 subject fields and is mainly published in 32 kinds of journals,which is dominated by natural science research( 1427 literatures). Researchers are mainly from each college and university,some design institutes and Chinese Academy of Sciences. The research could play certain guidance significance for further research and construction of ecological city construction in China.展开更多
Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the presen...Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.展开更多
Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sa...Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sani People,and present the long history and brilliant culture of Sani People. These culture resources could be used to expand the cultural aspects of leisure agriculture,to solve the problems of low participation and less distinct characteristics. This study can provide a reference for building the high-end leisure agriculture with distinct characteristics of Yunnan Province,to form a new growth point for regional growth.展开更多
Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutr...Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutral red （ Treatment T3 ） on the biomass, root-shoot ratio, leaf color indices, plant carbon and nitrogen nutrition were studied. The results showed that the biomass of Treatment T3 was significantly greater than that of treatments T1 and CK. The root-shoot ratio decreased significantly in treatments T1, T2 and T3 , and the decrease in T3 was most obvious. In all the three treatments with coloring agent, a ^＊ , b ^＊ and L ^＊ values were increased gradually, C value were decreased, H0 and CIRG were increased, and the leaves were pink. In addition, the contents of chlorophyll a, chlorophyll b, chlorophyll a ＋ b and carotenoid were significantly decreased. The contents of soluble sugar and starch were also decreased, and the decrease in Treatment T2 was most significant. The contents of soluble protein and total nitrogen were increased, and the increase was most dramatic in Treatment T3. The carbon to nitrogen ratio was decreased. The results proved that staining can improve the ornamental value of indoor plants, despite its effects on plant carbon and nitrogen nutrition of C. comosum vat. variegatum, dyeing.展开更多
[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 ...[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq.展开更多
Objective:This study aims to develop prognostic nomograms based on inflammatory markers for hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)patients.Methods:Between November 2003 and December 2017,1,350HCC...Objective:This study aims to develop prognostic nomograms based on inflammatory markers for hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)patients.Methods:Between November 2003 and December 2017,1,350HCC patients who were HBV surface antigen(HBsAg)positive and underwent curative hepatectomy were retrospectively collected from two medical centers.展开更多
Objective:Tumor size and location may have highly important influence on the treatment choices for hepatocellular carcinoma(HCC).This study aimed to investigate the optimal treatment modality between radiofrequency ab...Objective:Tumor size and location may have highly important influence on the treatment choices for hepatocellular carcinoma(HCC).This study aimed to investigate the optimal treatment modality between radiofrequency ablation(RFA)and repeat hepatectomy according to tumor size and location for solitary small recurrent HCC(rHCC).展开更多
Objective:Treatment strategies for recurrent hepatocellular carcinoma(rHCC)are controversial.We used the status of microvascular invasion(MVI)at primary resection as a marker to choose moderate treatment options for r...Objective:Treatment strategies for recurrent hepatocellular carcinoma(rHCC)are controversial.We used the status of microvascular invasion(MVI)at primary resection as a marker to choose moderate treatment options for rHCC patients with Barcelona Clinic Liver Cancer(BCLC)stage B-C disease.Methods:From June 2009 to June 2017.展开更多
[Objectives]This study was conducted to enrich the plant resource of ornamental vegetables in Kunming. [Methods]15 leafy vegetables were selected as test materials in this study. This study investigated the biological...[Objectives]This study was conducted to enrich the plant resource of ornamental vegetables in Kunming. [Methods]15 leafy vegetables were selected as test materials in this study. This study investigated the biological characteristics of the 15 ornamental vegetables by introduction trial and grey correlation analysis. In addition,we discussed the application of ornamental vegetables in urban agriculture. [Results]The grey correlation values of 11 ornamental vegetables were greater than 0. 6. The grey correlation values of other four ornamental vegetables were less than 0. 5. Among them,the grey correlation of Beta vulgaris L. var. cicla L. was 0. 94,which have the highest appreciation evaluation. The second appreciation evaluation belonged to rainbow leafy Beta vulgaris L. var. cicla L. with grey correlation of 0. 88. [Conclusions] In short,there are nine ornamental vegetables could be applied in the urban agriculture of Kunming,and there are six ornamental vegetables must be further investigated by cultivation experiment,to understand their cultivation characteristics and key points of management.展开更多
[Objective] This study aimed to investigate the diversity of community composition and diversity of endophytic bacteria in Anoectochilus roxburghii.[Method] By using Illumina MiSeq high-throughput sequencing technolo...[Objective] This study aimed to investigate the diversity of community composition and diversity of endophytic bacteria in Anoectochilus roxburghii.[Method] By using Illumina MiSeq high-throughput sequencing technology, the 16S rDNA-V4 region of endophytic bacteria in ^4. roxburghii was sequenced. Qiime and Mothur software packages were used to sort and count the number of sequences and operational taxonomic units （OTUs） and analyze the species abundance and distribution, Alpha diversity and differences of species abundance among samples. [Result] The number of effective sequences/OTUs for each sample was 110485/247 （stem） and 98524/259 （leaf） , respectively. The rarefaction curve showed that adequate sampling was performed. The number of OTUs had almost reached a saturation point. Endophytic bacteria in A. roxburghii mainly belonged to the genera Acidovorax, Pseudomonas, Sphingomonas and Halomonas. [Conclusions] Illumi-na MiSeq high-throughput sequencing technology provides more accurate and scientific data resources for the study of endophytic bacteria in A. roxburghii.展开更多
The Nineteenth National Congress of the Communist Party of China has defined the mission of educating people for our educators.The style of study reflects the education philosophy,culture and spirit of a university fr...The Nineteenth National Congress of the Communist Party of China has defined the mission of educating people for our educators.The style of study reflects the education philosophy,culture and spirit of a university from the inside to the outside. Starting from the present situation and existing problems of the construction of the style of study in colleges and universities in the new period,this paper puts forward some recommendations for the construction of the style of study in colleges and universities in the new period.展开更多
Sponge city construction,as an important measure of ecological construction in China,can effectively solve a series of problems such as large annual runoff,serious non-point source pollution,regional water shortage,lo...Sponge city construction,as an important measure of ecological construction in China,can effectively solve a series of problems such as large annual runoff,serious non-point source pollution,regional water shortage,low utilization rate of rain and flood resources,unsustainable ecological benefits of water landscape and poor landscape functions in urbanization construction. This paper uses LID concept to construct sponge city in the old urban area of Kunming,to reduce the total runoff and promote the utilization of rainfall and flood resources so as to rebuild,upgrade and optimize the regional environment and provide some reference for the regional construction of small-scale sponge city.展开更多
Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a m...Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a major tobacco planting area in Yunan, showed that： ① More than 80% of the pesticides purchased by farmers were used for tobacco cultivation, which was 4 times that of conventional crops planting, and most of the packaging materials were plastic bags; ② The main way for farmers to dispose plastic bags was to burn or discard them in the field garbage collection baskets; ③ In combination with field surveys, an average of 4.54 pieces of pesticide packaging wastes were scattered around every 667 m^2 of tobacco field. In addition, the number reduced significantly after carrying out pesticide packaging wastes recycling and control project at the local smoke station. At the same time, the average number of pesticide packaging wastes in the field collection baskets increased. Therefore, it can effectively control pesticide packaging wastes by changing the type of pesticide packaging, encouraging the establishment of recycling system and strengthening the cognitive training of farmers.展开更多
[Objectives] To explore the diversity of endophytes in Moringa oleifera,and provide a scientific basis for better protection,development and use of Moringa oleifera resources. [Methods] With Moringa oleifera stems and...[Objectives] To explore the diversity of endophytes in Moringa oleifera,and provide a scientific basis for better protection,development and use of Moringa oleifera resources. [Methods] With Moringa oleifera stems and leaves as materials,the Illumina Mi Seq highthroughput sequencing technology was used to study the endophyte diversity. [Results] A total of 199 OTUs were measured in the samples,belonging to 12 phyla such as Proteobacteria,Actinobacteria,Acidobacteria,Gemmatimonadetes,Bacteroidetes,Chloroflexi,DeinococcusThermus,Verrucomicrobia,Candidatus Saccharibacteria and Cyanobacteria. [Conclusions]Gemmatimonas is the dominant population of endophyte in Moringa oleifera.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52170070,52400022 and 52200088)the Youth S&T Talent Support Programme of Guangdong Provincial Association for Science and Technology(GDSTA)(No.SKXRC202406)+1 种基金China Postdoctoral Science Foundation(No.2023M740754)“One hundred Youth”Science and Technology Plan,Guangdong University of Technology,China(No.263113906).
文摘As part of sewage treatment,coagulation could remove phosphorus from the effluent of the Anaerobic-Anoxic-Oxic-Anoxic(A^(2)OA)biological process.The importance in investigating the influence of coagulation on Anaerobic-Anoxic-Oxic-Anoxic Membrane Bioreactor(A^(2)OA-MBR)should be emphasized.In this study,systematic optimization of coagulation parameters for greater pollutant removal was conducted in terms of coagulant dosage,coagulation residence time and stirring hydraulic conditions.Coagulation process could remarkably remove turbidity,phosphorus,chemical oxygen demand,humic-like,protein-like and polysaccharide-like substances from secondary effluent and A^(2)OA sludge-liquid mixture.Furthermore,the influence of coagulation on membrane fouling development during the ultrafiltration of secondary effluent and A^(2)OA sludge-liquid mixture was investigated based on optimum coagulation parameters.Coagulation simultaneously reduced reversible membrane fouling and the irreversible one by 86%and 16%,respectively.According to excitation-emission matrix and attenuated total reflection-fourier transform infrared spectra,membrane fouling was primarily influenced by the cake layer,although pore fouling might be aggravated by A^(2)OA processes.Besides,the feasibility of coagulation-assisted A^(2)OA-MBRwas also assessed using hollowfibermembranes.It exhibited excellent potential in alleviatingmembrane fouling,while regular cleaning twice a day was not enough to suppress transmembrane pressure increase during direct domestic wastewater ultrafiltration.Additionally,both polysaccharide-like and protein-like foulants were vital components for membrane fouling during wastewater treatment.
基金Supported by Key Project of Yunnan Provincial Science and Technology Plan(202202AE090015)Scientific Research Fund of Yunnan Education Department(2024Y742+3 种基金2023Y0863)National Natural Science Foundation of China(42067009)2023 Undergraduate Innovation and Entrepreneurship Training Program of Yunnan Education Department(S202311393044S202311393061).
文摘Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.
基金Supported by National Natural Science Foundation of China(42067009)Scientific Research Project of Yunnan Provincial Department of Education(2024Y742,2023Y0863)+2 种基金Project of Kunming Municipal Commission of Development and Reform(Kunming Spring City Industrial Technology Leading Talents)Science and Technology Major Project of Yunnan Province Science and Technology Department(202202AE090015-02)Yunnan Students’innovation and entrepreneurship training program(S202311393044,S202311393061)。
文摘Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.
文摘The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection in the field of blasting.Serverless Computing can provide a variety of computing services for people without hardware foundations and rich software development experience,which has aroused people’s interest in how to use it in the field ofmachine learning.In this paper,we design a distributedmachine learning training application based on the AWS Lambda platform.Based on data parallelism,the data aggregation and training synchronization in Function as a Service(FaaS)are effectively realized.It also encrypts the data set,effectively reducing the risk of data leakage.We rent a cloud server and a Lambda,and then we conduct experiments to evaluate our applications.Our results indicate the effectiveness,rapidity,and economy of distributed training on FaaS.
基金Supported by Scientific Research Fund of Yunnan Education Department(2024Y742,2023Y0863)National Natural Science Foundation of China(42067009)+1 种基金College Students'Innovative Training Plan Program of Yunnan Education Department in 2023(S202311393044,S202311393061)Key Project of Science and Technology Program of Yunnan Province(202202AE090015).
文摘At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.
文摘Objective:Preoperative prediction of microvascular invasion(MVI)in patients with hepatocellular cancer(HCC)is important for developing surgical strategy.We aimed to develop and validate a combined intratumoral and peritumoral radiomics model based on gadolinium-ethoxybenzyl-diethylenetriaminepentaacetic acid(Gd-EOB-DTPA)-enhanced magnetic resonance imaging(MRI)for preoperative prediction of MVI in primary HCC patients.
基金Supported by Science Research Fund of Yunnan Provincial Education Department(2017ZZX090)School-level Key Project of Kunming University(XJZD1602)"Study on Key Technology of Ecological Planting Mode of Balcony Vegetables"of Provincial-level"Quality Project"of Kunming University-Innovation and Entrepreneurship Training Program for College Students
文摘To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and other retrieval tools,the statistics and analysis of 3152 research literatures on sponge city published in domestic academic journals of 2004-2016 are conducted based on bibliometrics. It is found that since the concept of " sponge city" was firstly proposed in 2012,development research of sponge city involves 40 subject fields and is mainly published in 32 kinds of journals,which is dominated by natural science research( 1427 literatures). Researchers are mainly from each college and university,some design institutes and Chinese Academy of Sciences. The research could play certain guidance significance for further research and construction of ecological city construction in China.
基金Supported by National Natural Science Foundation of China(31301665)
文摘Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.
基金Supported by Science and Technology Project Research of Kunming University "Study on Yunnan Leisure Agriculture Cultural Origin"(XJL13021)Yunnan Province Urban Leisure Agriculture Engineering Technology Research Center Project "Study on Family Leisure Farm Circular Agriculture Development Model"(XXNY1410)+2 种基金Construction Project of Key Superior and Characteristic Disciplines(Ecology) in Colleges and Universities of Yunnan ProvinceScientific Research Foundation of Yunnan Provincial Department of Education "Study on Ecological Reconstruction of Old City Based on Low Impact Development Target"(2017ZZX090)Science and Technology Project Research of Kunming University "Study on LID System Theory Construction of Sponge City Landscape Corridor"(XJZD1602)
文摘Through exploring the material and nonmaterial cultural resources in leisure agriculture of Sani People in Shilin of Yunnan Province,this paper introduced festivals,marriage,traditional clothing,and food culture of Sani People,and present the long history and brilliant culture of Sani People. These culture resources could be used to expand the cultural aspects of leisure agriculture,to solve the problems of low participation and less distinct characteristics. This study can provide a reference for building the high-end leisure agriculture with distinct characteristics of Yunnan Province,to form a new growth point for regional growth.
文摘Using potted seedlings of Chlorophytum comosum var. variegatum as the experimental materials, the effect of 2.0 mmol/L methyl orange （ Treatment T1 ）, 1.0 mmol/L methyl violet （ Treatment T2 ） and 1.0 mmol/L neutral red （ Treatment T3 ） on the biomass, root-shoot ratio, leaf color indices, plant carbon and nitrogen nutrition were studied. The results showed that the biomass of Treatment T3 was significantly greater than that of treatments T1 and CK. The root-shoot ratio decreased significantly in treatments T1, T2 and T3 , and the decrease in T3 was most obvious. In all the three treatments with coloring agent, a ^＊ , b ^＊ and L ^＊ values were increased gradually, C value were decreased, H0 and CIRG were increased, and the leaves were pink. In addition, the contents of chlorophyll a, chlorophyll b, chlorophyll a ＋ b and carotenoid were significantly decreased. The contents of soluble sugar and starch were also decreased, and the decrease in Treatment T2 was most significant. The contents of soluble protein and total nitrogen were increased, and the increase was most dramatic in Treatment T3. The carbon to nitrogen ratio was decreased. The results proved that staining can improve the ornamental value of indoor plants, despite its effects on plant carbon and nitrogen nutrition of C. comosum vat. variegatum, dyeing.
基金Supported by Scientific Research Foundation of Yunnan Provincial Department of Education(2015Y393)Scientific Research Project of Kunming University(XJZD1605)+1 种基金Construction Project of Key Superior and Characteristic Disciplines(Ecology)in Colleges and Universities of Yunnan Province(05000511311)Project of Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXKJ201622)
文摘[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq.
文摘Objective:This study aims to develop prognostic nomograms based on inflammatory markers for hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)patients.Methods:Between November 2003 and December 2017,1,350HCC patients who were HBV surface antigen(HBsAg)positive and underwent curative hepatectomy were retrospectively collected from two medical centers.
文摘Objective:Tumor size and location may have highly important influence on the treatment choices for hepatocellular carcinoma(HCC).This study aimed to investigate the optimal treatment modality between radiofrequency ablation(RFA)and repeat hepatectomy according to tumor size and location for solitary small recurrent HCC(rHCC).
文摘Objective:Treatment strategies for recurrent hepatocellular carcinoma(rHCC)are controversial.We used the status of microvascular invasion(MVI)at primary resection as a marker to choose moderate treatment options for rHCC patients with Barcelona Clinic Liver Cancer(BCLC)stage B-C disease.Methods:From June 2009 to June 2017.
文摘[Objectives]This study was conducted to enrich the plant resource of ornamental vegetables in Kunming. [Methods]15 leafy vegetables were selected as test materials in this study. This study investigated the biological characteristics of the 15 ornamental vegetables by introduction trial and grey correlation analysis. In addition,we discussed the application of ornamental vegetables in urban agriculture. [Results]The grey correlation values of 11 ornamental vegetables were greater than 0. 6. The grey correlation values of other four ornamental vegetables were less than 0. 5. Among them,the grey correlation of Beta vulgaris L. var. cicla L. was 0. 94,which have the highest appreciation evaluation. The second appreciation evaluation belonged to rainbow leafy Beta vulgaris L. var. cicla L. with grey correlation of 0. 88. [Conclusions] In short,there are nine ornamental vegetables could be applied in the urban agriculture of Kunming,and there are six ornamental vegetables must be further investigated by cultivation experiment,to understand their cultivation characteristics and key points of management.
基金Supported by National Natural Science Foundation of China(31460491)Youth Project of Applied Basic Research Program of Yunnan Provincial Science and Technology Department(2013FD040)+2 种基金Scientific Research Project of Education Department of Yunnan Province(2014Y390)Talent Introduction Project of Kunming University(YJL14005)Project of Key Characteristic Discipline(Ecology)Construction for Colleges and Universities in Yunnan Province(05000511311)
文摘[Objective] This study aimed to investigate the diversity of community composition and diversity of endophytic bacteria in Anoectochilus roxburghii.[Method] By using Illumina MiSeq high-throughput sequencing technology, the 16S rDNA-V4 region of endophytic bacteria in ^4. roxburghii was sequenced. Qiime and Mothur software packages were used to sort and count the number of sequences and operational taxonomic units （OTUs） and analyze the species abundance and distribution, Alpha diversity and differences of species abundance among samples. [Result] The number of effective sequences/OTUs for each sample was 110485/247 （stem） and 98524/259 （leaf） , respectively. The rarefaction curve showed that adequate sampling was performed. The number of OTUs had almost reached a saturation point. Endophytic bacteria in A. roxburghii mainly belonged to the genera Acidovorax, Pseudomonas, Sphingomonas and Halomonas. [Conclusions] Illumi-na MiSeq high-throughput sequencing technology provides more accurate and scientific data resources for the study of endophytic bacteria in A. roxburghii.
文摘The Nineteenth National Congress of the Communist Party of China has defined the mission of educating people for our educators.The style of study reflects the education philosophy,culture and spirit of a university from the inside to the outside. Starting from the present situation and existing problems of the construction of the style of study in colleges and universities in the new period,this paper puts forward some recommendations for the construction of the style of study in colleges and universities in the new period.
基金Supported by Science Research Fund of Yunnan Provincial Department of Education(2017ZZX090)Key Scientific Research Project of University(XJZD1602)Open Fund Project of Key Laboratories for the Opening and Utilization of Characteristic Biological Resources in Colleges and Universities of Yunnan Province,Kunming University(GXKZ201714)
文摘Sponge city construction,as an important measure of ecological construction in China,can effectively solve a series of problems such as large annual runoff,serious non-point source pollution,regional water shortage,low utilization rate of rain and flood resources,unsustainable ecological benefits of water landscape and poor landscape functions in urbanization construction. This paper uses LID concept to construct sponge city in the old urban area of Kunming,to reduce the total runoff and promote the utilization of rainfall and flood resources so as to rebuild,upgrade and optimize the regional environment and provide some reference for the regional construction of small-scale sponge city.
基金Supported by the Science and Technology Planning Project of Yunan Tobacco Co.,Ltd.(2015YN36)
文摘Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a major tobacco planting area in Yunan, showed that： ① More than 80% of the pesticides purchased by farmers were used for tobacco cultivation, which was 4 times that of conventional crops planting, and most of the packaging materials were plastic bags; ② The main way for farmers to dispose plastic bags was to burn or discard them in the field garbage collection baskets; ③ In combination with field surveys, an average of 4.54 pieces of pesticide packaging wastes were scattered around every 667 m^2 of tobacco field. In addition, the number reduced significantly after carrying out pesticide packaging wastes recycling and control project at the local smoke station. At the same time, the average number of pesticide packaging wastes in the field collection baskets increased. Therefore, it can effectively control pesticide packaging wastes by changing the type of pesticide packaging, encouraging the establishment of recycling system and strengthening the cognitive training of farmers.
基金Supported by Scientific Research Project of Yunnan Provincial Department of Education(2015Y393)Open Project of Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXKJ201622)Scientific Research Project for Students in Kunming University(XJD16082)
文摘[Objectives] To explore the diversity of endophytes in Moringa oleifera,and provide a scientific basis for better protection,development and use of Moringa oleifera resources. [Methods] With Moringa oleifera stems and leaves as materials,the Illumina Mi Seq highthroughput sequencing technology was used to study the endophyte diversity. [Results] A total of 199 OTUs were measured in the samples,belonging to 12 phyla such as Proteobacteria,Actinobacteria,Acidobacteria,Gemmatimonadetes,Bacteroidetes,Chloroflexi,DeinococcusThermus,Verrucomicrobia,Candidatus Saccharibacteria and Cyanobacteria. [Conclusions]Gemmatimonas is the dominant population of endophyte in Moringa oleifera.
