“食药同源”的思想在中国历史中占据着无可比拟的重要地位。在此次新冠肺炎疫情中,“食药同源”以药膳的形式走进千家万户,为人民健康构筑起坚固防线,更为我国中医药产业逐步迈向国际市场奠定了基础。但市场上食药同源产品良莠不齐、...“食药同源”的思想在中国历史中占据着无可比拟的重要地位。在此次新冠肺炎疫情中,“食药同源”以药膳的形式走进千家万户,为人民健康构筑起坚固防线,更为我国中医药产业逐步迈向国际市场奠定了基础。但市场上食药同源产品良莠不齐、以次充好和假冒伪劣等现象屡见不鲜。食药同源产品掺假手段复杂多样,食药同源产品的溯源技术研究逐渐成为研究热点。产地溯源技术以保障食药同源产品的“道地性”和“特色性”为主要目的,同时,维护消费者权益,保护食药同源产品的真实性和食药同源产业的健康发展。该文以截至2021年6月的中文数据库知网、英文数据库Web of Sciences和Pud Med收录的期刊、硕博论文为基础数据,分析了溯源技术在食药同源产品的应用研究进展,并对未来发展趋势进行了展望,为今后食药同源产品产地溯源技术研究提供理论依据。展开更多
Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting meth...Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting methods,the CCK-8 assay,transwell migration assay and JC-1 staining,were used to observe the effects of the combination on the proliferation,migration and apoptosis of NPC stem cells,respectively.Western blot was used to detect the levels of protein in NPC-SCs.Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPCSC apoptosis.Mechanistically,YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins,including cleaved Caspase-3,cleaved Caspase-7 and cleaved Caspase-9.Moreover,YQJDF combined with SAL synergistically decreased the levels of CD44,p-c-Src,Ras,p-PKCδ,p-MEK,p-c-Raf,p-ERK1/2 and p-AKT proteins.Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis,which may be closely related to the downregulation of the CD44/Ras signaling pathway.展开更多
文摘“食药同源”的思想在中国历史中占据着无可比拟的重要地位。在此次新冠肺炎疫情中,“食药同源”以药膳的形式走进千家万户,为人民健康构筑起坚固防线,更为我国中医药产业逐步迈向国际市场奠定了基础。但市场上食药同源产品良莠不齐、以次充好和假冒伪劣等现象屡见不鲜。食药同源产品掺假手段复杂多样,食药同源产品的溯源技术研究逐渐成为研究热点。产地溯源技术以保障食药同源产品的“道地性”和“特色性”为主要目的,同时,维护消费者权益,保护食药同源产品的真实性和食药同源产业的健康发展。该文以截至2021年6月的中文数据库知网、英文数据库Web of Sciences和Pud Med收录的期刊、硕博论文为基础数据,分析了溯源技术在食药同源产品的应用研究进展,并对未来发展趋势进行了展望,为今后食药同源产品产地溯源技术研究提供理论依据。
基金We thank for the funding support from the National Natural Science Foundation of China(No.81874408,No.81973914 and No.81573721)the Natural Science Foundation of Hunan Province,China(No.2019JJ40216)Study Fundation of Hunan Provincial Education Department(No.19B439).
文摘Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting methods,the CCK-8 assay,transwell migration assay and JC-1 staining,were used to observe the effects of the combination on the proliferation,migration and apoptosis of NPC stem cells,respectively.Western blot was used to detect the levels of protein in NPC-SCs.Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPCSC apoptosis.Mechanistically,YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins,including cleaved Caspase-3,cleaved Caspase-7 and cleaved Caspase-9.Moreover,YQJDF combined with SAL synergistically decreased the levels of CD44,p-c-Src,Ras,p-PKCδ,p-MEK,p-c-Raf,p-ERK1/2 and p-AKT proteins.Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis,which may be closely related to the downregulation of the CD44/Ras signaling pathway.
