Lysobacter harbors a plethora of cryptic biosynthetic gene clusters(BGCs),albeit only a limited number have been analyzed to date.In this study,we described the activation of a cryptic polyketide synthase(PKS)/nonribo...Lysobacter harbors a plethora of cryptic biosynthetic gene clusters(BGCs),albeit only a limited number have been analyzed to date.In this study,we described the activation of a cryptic polyketide synthase(PKS)/nonribosomal peptide synthetase(NRPS)gene cluster(lsh)in Lysobacter sp.DSM 3655 through promoter engineering and heterologous expression in Streptomyces sp.S001.As a result of this methodology,we were able to isolate two novel linear lipopeptides,lysohexaenetides A(1)and B(2),from the recombinant strain S001-lsh.Furthermore,we proposed the biosynthetic pathway for lysohexaenetides and identified LshA as another example of entirely iterative bacterial PKSs.This study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes,particularly in the absence of genetic manipulation tools.展开更多
In order to study the thermal and moisture comfort properties of down jacket fabrics,20 popular down jacket fabrics were selected from the market.The basic specifications of the samples were tested,and thermal and moi...In order to study the thermal and moisture comfort properties of down jacket fabrics,20 popular down jacket fabrics were selected from the market.The basic specifications of the samples were tested,and thermal and moisture performance tests were conducted.The obtained thermal and moisture indicators were analyzed,and then combined with the subjective evaluation results to obtain the regression equation for the evaluation of the comprehensive thermal and moisture comfort of down jacket fabrics.The air permeability,the thermal resistance,the warm and cold feeling and the moisture permeability were tested,and the results were divided into 5 levels.The results showed that the consistency of the air permeability and the moisture permeability of down jacket fabrics studied was better,and the thermal insulation of the down jacket fabrics with coating was better.The thickness of the down jacket fabrics had an effect on the warm and cold feeling of instant contact,and the consistency of subjective and objective warm and cold feeling was better.The obtained comprehensive evaluation equation of the thermal and moisture comfort has certain guiding significance for the thermal and moisture comfort of down jacket fabrics.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81573311 and 82173702).
文摘Lysobacter harbors a plethora of cryptic biosynthetic gene clusters(BGCs),albeit only a limited number have been analyzed to date.In this study,we described the activation of a cryptic polyketide synthase(PKS)/nonribosomal peptide synthetase(NRPS)gene cluster(lsh)in Lysobacter sp.DSM 3655 through promoter engineering and heterologous expression in Streptomyces sp.S001.As a result of this methodology,we were able to isolate two novel linear lipopeptides,lysohexaenetides A(1)and B(2),from the recombinant strain S001-lsh.Furthermore,we proposed the biosynthetic pathway for lysohexaenetides and identified LshA as another example of entirely iterative bacterial PKSs.This study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes,particularly in the absence of genetic manipulation tools.
基金Natural Science Foundation Project of Shanghai 2020“Science and Technology Innovation Action Plan”,China(No.20ZR1400200)Jiangxi Provincial Bureau for Quality and Technical Supervision,China(No.GZJKY201807)Jiangxi Provincial Administration for Market Regulation,China(No.GSJK201909)。
文摘In order to study the thermal and moisture comfort properties of down jacket fabrics,20 popular down jacket fabrics were selected from the market.The basic specifications of the samples were tested,and thermal and moisture performance tests were conducted.The obtained thermal and moisture indicators were analyzed,and then combined with the subjective evaluation results to obtain the regression equation for the evaluation of the comprehensive thermal and moisture comfort of down jacket fabrics.The air permeability,the thermal resistance,the warm and cold feeling and the moisture permeability were tested,and the results were divided into 5 levels.The results showed that the consistency of the air permeability and the moisture permeability of down jacket fabrics studied was better,and the thermal insulation of the down jacket fabrics with coating was better.The thickness of the down jacket fabrics had an effect on the warm and cold feeling of instant contact,and the consistency of subjective and objective warm and cold feeling was better.The obtained comprehensive evaluation equation of the thermal and moisture comfort has certain guiding significance for the thermal and moisture comfort of down jacket fabrics.
