Seawater salinity is greatly influenced by tide, evaporation and rain falls. In this study, we investigated the growth and photosynthetic responses of zygote-derived Ulva fasciata Delile germlings to short-term （minu...Seawater salinity is greatly influenced by tide, evaporation and rain falls. In this study, we investigated the growth and photosynthetic responses of zygote-derived Ulva fasciata Delile germlings to short-term （minutes） and prolonged （days） exposure to different salinity gradients, to evaluate the effect of salinity variation on the early stage of life history in this seaweed. The results showed that, the maximum net photosynthetic rates （NPRm） of U. fasciata germlings was observably decreased in desalted （25 and 15） and high （45） salinity seawater in short-term exposure tests （in minutes）. However, after 30 min, the photosynthesis activity in medium salinity （25） was maintained at a relative high level （above 70%）. After 8 d prolonged culture, the photosynthesis and mean relative growth rate （RGR） of germlings were all markedly lowered, whereas the malondialdehyde （MDA） contents increased as the salinity desalted from 34 to 15. The salinity decrease from 34 to 25 had no significant effect on the RGR, but obviously influenced the morphology of the germlings. High salinity level （45） significantly depressed the RGR and photosynthesis of U. fasciata germlings, while it notably increased the MDA contents. The results showed that the salinity elevation had more detrimental effects on Ulvafasciata germlings than salinity decrease did. The germlings grown at the salinity seawater levels from 25 to 34, performed preferable photosynthetic acclimation both in temporary and prolonged culture. Broad salinity tolerance from 25 to 34 in U. fasciata germlings may have partly evolved as a response to regular diurnal tides.展开更多
Dark respiration （non-photorespiratory mitochondrial respiration）, which occurs both in the light and in darkness, is vital for growth and survival of algae and plays a critical role in modulating the carbon balance...Dark respiration （non-photorespiratory mitochondrial respiration）, which occurs both in the light and in darkness, is vital for growth and survival of algae and plays a critical role in modulating the carbon balance of them. In the present study, we have investigated dark respiration in the light （RL） and in darkness （RD） in three marine macroalgal species, Hizikia fusiformis （phaeophyta）, Gracilaria lemaneiformis （Rhodophyta） and Ulva lactuca （Chlorophyta）, cultured at 20 ℃ using aeration with two CO2 conditions： current ambient （CO2 concentration about 380 μl/L） and elevated CO2 （approximately 720 μl/L） air. RL was estimated by using the Kok method, whereas RD was determined as the rate of O2 influx at zero light. The results showed that both RL and RD were unchanged for the elevated CO2-grown algae relative to ambient CO2 concentration for all the algal species tested. However, RL was significantly lower than RD across all the algal species and growth CO2 treatments, demonstrating that daytime respiration was partly depressed by the light. The percentage of inhibition of respiration by light was similar between ambient and elevated CO2- grown algae. The ratio of respiration to photosynthesis, which tended to decrease when estimated using RL instead of RD, was not altered for the elevated relative to ambient CO2 concentration. The results suggest that RL, rather than RD, is a more accurate estimate of nonphotorespiratory carbon loss in marine macroalgae during the daytime. It would not be anticipated that elevated atmospheric CO2 would exert a substantial influence on respiratory flux either in the light or in darkness in these particular marine macroalgal species.展开更多
Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental condit...Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental conditions, including different temperatures（15℃, 20℃, and 25℃）, sunlight intensities（100%, 50%, and 25% of full solar radiation） and types of UVR（UV-A and UV-B）. Then, we determined the lengths, maximal quantum yields（ Fv/Fm）, and survival rates of the embryos. The results showed that the embryos had the highest lengths, Fv/Fm values and survival percentages when cultured under a temperature of 20℃ and 25% sunlight intensity. The full level of solar radiation severely reduced the growth and survival ratios of the embryos. Hizikia embryos recovered within 2 h from the damage caused by short-term（30 min） high radiation（about 70% of full solar radiation） and UVR exposure. However, the viabilities of the embryos were significantly decreased after 4 h exposure to UVR. According to these results, we propose that culture conditions with indoor natural light and room temperature would be favorable for the growth, development and physiology of H izikia embryos.展开更多
China is the global leader in mariculture production. Increasing sequestered marine carbon(also known as blue carbon) via mariculture activities is a promising approach for mitigating climate change and promoting the ...China is the global leader in mariculture production. Increasing sequestered marine carbon(also known as blue carbon) via mariculture activities is a promising approach for mitigating climate change and promoting the development of a low-carbon economy. Mariculture blue carbon is also considered an important component of China's "sea granary". In addition to shellfish and macroalgae yields, which represent carbon removed from mariculture environment, blue carbon also includes other important components, which have been largely neglected in the past, such as the carbon transformed by microbes,dissolved organic carbon(mainly referred to as recalcitrant dissolved organic carbon), and sedimentary particulate carbon. Hence,from different aspects, a comprehensive study on the formation processes and mechanisms of carbon sequestration is of great significance for comprehensively unveiling the carbon sequestration capability in coastal mariculture environment, which will contribute to the sustainable development of the fishery economy and construction of an ecological civilization. Moreover, it may add significant economic benefits to the future carbon-trading market.展开更多
The activities of carbonic anhydrase (CA) and photosynthesis of Porphyra haitanensis were investigated in order to see its photosynthetic utilization of inorganic carbon source. Both intra- and extra-cellular CA activ...The activities of carbonic anhydrase (CA) and photosynthesis of Porphyra haitanensis were investigated in order to see its photosynthetic utilization of inorganic carbon source. Both intra- and extra-cellular CA activities existed in the thallus. CA inhibitors, acetazolamide (AZ) and ethoxy-zolamide (EZ), remarkably depressed the photosynthetic oxygen evolution in seawater of pH 8.2 and 10.0, and EZ showed stronger inhibition than AZ. The observed net photosynthetic rate in seawater of pH 8.2 was much higher than that of CO2 supply theoretically derived from spontaneous dehydration of HCO3. P. haitanensis also showed a rather high pH compensation point (9.9). The results demonstrated that P. haitanensis could utilize bicarbonate as the external inorganic carbon source for photosynthesis. The bicarbonateutilization was closely associated with HCO3- dehydrationcatalyzed by extracellular CA activity. The inorganic carbon composition in seawater could well saturate the photosynthesis of P. haitanensis. The展开更多
基金The National Natural Science Foundation of China under contract Nos 41276148 and 41076094
文摘Seawater salinity is greatly influenced by tide, evaporation and rain falls. In this study, we investigated the growth and photosynthetic responses of zygote-derived Ulva fasciata Delile germlings to short-term （minutes） and prolonged （days） exposure to different salinity gradients, to evaluate the effect of salinity variation on the early stage of life history in this seaweed. The results showed that, the maximum net photosynthetic rates （NPRm） of U. fasciata germlings was observably decreased in desalted （25 and 15） and high （45） salinity seawater in short-term exposure tests （in minutes）. However, after 30 min, the photosynthesis activity in medium salinity （25） was maintained at a relative high level （above 70%）. After 8 d prolonged culture, the photosynthesis and mean relative growth rate （RGR） of germlings were all markedly lowered, whereas the malondialdehyde （MDA） contents increased as the salinity desalted from 34 to 15. The salinity decrease from 34 to 25 had no significant effect on the RGR, but obviously influenced the morphology of the germlings. High salinity level （45） significantly depressed the RGR and photosynthesis of U. fasciata germlings, while it notably increased the MDA contents. The results showed that the salinity elevation had more detrimental effects on Ulvafasciata germlings than salinity decrease did. The germlings grown at the salinity seawater levels from 25 to 34, performed preferable photosynthetic acclimation both in temporary and prolonged culture. Broad salinity tolerance from 25 to 34 in U. fasciata germlings may have partly evolved as a response to regular diurnal tides.
基金The 973 Project under contract No. 2009CB421207the National Natural Science Foundation of China under contract Nos 41076094 and 30970450+1 种基金Program for New Century Excellent Talents in University under contract No. NCET-10-0375the Fundamental Research Funds for the Central Universities,SCUT
文摘Dark respiration （non-photorespiratory mitochondrial respiration）, which occurs both in the light and in darkness, is vital for growth and survival of algae and plays a critical role in modulating the carbon balance of them. In the present study, we have investigated dark respiration in the light （RL） and in darkness （RD） in three marine macroalgal species, Hizikia fusiformis （phaeophyta）, Gracilaria lemaneiformis （Rhodophyta） and Ulva lactuca （Chlorophyta）, cultured at 20 ℃ using aeration with two CO2 conditions： current ambient （CO2 concentration about 380 μl/L） and elevated CO2 （approximately 720 μl/L） air. RL was estimated by using the Kok method, whereas RD was determined as the rate of O2 influx at zero light. The results showed that both RL and RD were unchanged for the elevated CO2-grown algae relative to ambient CO2 concentration for all the algal species tested. However, RL was significantly lower than RD across all the algal species and growth CO2 treatments, demonstrating that daytime respiration was partly depressed by the light. The percentage of inhibition of respiration by light was similar between ambient and elevated CO2- grown algae. The ratio of respiration to photosynthesis, which tended to decrease when estimated using RL instead of RD, was not altered for the elevated relative to ambient CO2 concentration. The results suggest that RL, rather than RD, is a more accurate estimate of nonphotorespiratory carbon loss in marine macroalgae during the daytime. It would not be anticipated that elevated atmospheric CO2 would exert a substantial influence on respiratory flux either in the light or in darkness in these particular marine macroalgal species.
基金Supported by the Science and Technology Planning Project of Guangdong,China(No.2016A020222001)
文摘Understanding the embryo physiology of Hizikia fusiformis（Harvey） Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental conditions, including different temperatures（15℃, 20℃, and 25℃）, sunlight intensities（100%, 50%, and 25% of full solar radiation） and types of UVR（UV-A and UV-B）. Then, we determined the lengths, maximal quantum yields（ Fv/Fm）, and survival rates of the embryos. The results showed that the embryos had the highest lengths, Fv/Fm values and survival percentages when cultured under a temperature of 20℃ and 25% sunlight intensity. The full level of solar radiation severely reduced the growth and survival ratios of the embryos. Hizikia embryos recovered within 2 h from the damage caused by short-term（30 min） high radiation（about 70% of full solar radiation） and UVR exposure. However, the viabilities of the embryos were significantly decreased after 4 h exposure to UVR. According to these results, we propose that culture conditions with indoor natural light and room temperature would be favorable for the growth, development and physiology of H izikia embryos.
基金supported by the National Key Research and Development Program of China(Grant No.2016YFA0601402)the Open Task of Qingdao National Laboratory for Marine Science and Technology(Grant No.QNLM2016ORP0311)the Key R&D Project in Shandong Province(Grant No.2015GSF115036)
文摘China is the global leader in mariculture production. Increasing sequestered marine carbon(also known as blue carbon) via mariculture activities is a promising approach for mitigating climate change and promoting the development of a low-carbon economy. Mariculture blue carbon is also considered an important component of China's "sea granary". In addition to shellfish and macroalgae yields, which represent carbon removed from mariculture environment, blue carbon also includes other important components, which have been largely neglected in the past, such as the carbon transformed by microbes,dissolved organic carbon(mainly referred to as recalcitrant dissolved organic carbon), and sedimentary particulate carbon. Hence,from different aspects, a comprehensive study on the formation processes and mechanisms of carbon sequestration is of great significance for comprehensively unveiling the carbon sequestration capability in coastal mariculture environment, which will contribute to the sustainable development of the fishery economy and construction of an ecological civilization. Moreover, it may add significant economic benefits to the future carbon-trading market.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39830060)the Natural Science Foundation of Guangdong Province.
文摘The activities of carbonic anhydrase (CA) and photosynthesis of Porphyra haitanensis were investigated in order to see its photosynthetic utilization of inorganic carbon source. Both intra- and extra-cellular CA activities existed in the thallus. CA inhibitors, acetazolamide (AZ) and ethoxy-zolamide (EZ), remarkably depressed the photosynthetic oxygen evolution in seawater of pH 8.2 and 10.0, and EZ showed stronger inhibition than AZ. The observed net photosynthetic rate in seawater of pH 8.2 was much higher than that of CO2 supply theoretically derived from spontaneous dehydration of HCO3. P. haitanensis also showed a rather high pH compensation point (9.9). The results demonstrated that P. haitanensis could utilize bicarbonate as the external inorganic carbon source for photosynthesis. The bicarbonateutilization was closely associated with HCO3- dehydrationcatalyzed by extracellular CA activity. The inorganic carbon composition in seawater could well saturate the photosynthesis of P. haitanensis. The
