The apple rootstock, A106 (Malus sieboldii), had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell. Karyotypes were prepared from root-tip cells with 2n= 34 chromos...The apple rootstock, A106 (Malus sieboldii), had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell. Karyotypes were prepared from root-tip cells with 2n= 34 chromosomes. Seven out of 82 karyotypes (8.5%) showed one pair of satellites at the end of the short arm of chromosome 3. C-bands were shown on 6 pairs of chromosomes 2, 4,6, 8, 14, and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica: endopolygalacturonase (EPG,0. 6 kb ) , ACC oxidase (1.2 kb), and ACC synthase (2 kb) were hybridized in situ to metaphase chromosomes of A106. Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11. For the ACC oxidase gene, hybridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively proximal to the centromere of chromosome 1 in 81% of the spreads, and on the long arm of chromosome 13 in 50% of the spreads. Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads, chromosomes 9 and 10 in 76% of the spreads, and chromosome 17 in 56% of the spreads.展开更多
A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicini...A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chro-mosomes 6 and 14. The in situ results using digoxygenin labelling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cyto-genetic analysis of very small chromosomes (1.0-2.7μm)of apple.展开更多
文摘The apple rootstock, A106 (Malus sieboldii), had 17 bivalents in pollen mother cells at meiotic metaphase 1,and 17 chromosomes in a haploid pollen cell. Karyotypes were prepared from root-tip cells with 2n= 34 chromosomes. Seven out of 82 karyotypes (8.5%) showed one pair of satellites at the end of the short arm of chromosome 3. C-bands were shown on 6 pairs of chromosomes 2, 4,6, 8, 14, and 16 near the telomeric regions of short arms.Probes for three ripening-related genes from Malus x domestica: endopolygalacturonase (EPG,0. 6 kb ) , ACC oxidase (1.2 kb), and ACC synthase (2 kb) were hybridized in situ to metaphase chromosomes of A106. Hybridization sites for the EPG gene were observed on the long arm of chromosome 14 in 15 out of 16 replicate spreads and proximal to the centromere of chromosomes 6 and 11. For the ACC oxidase gene, hybridization sites were observed in the telomeric region of the short arm of chromosomes 5 and 11 in 87% and 81% of 16 spreads respectively proximal to the centromere of chromosome 1 in 81% of the spreads, and on the long arm of chromosome 13 in 50% of the spreads. Twenty five spreads were studied for the ACC synthase gene and hybridization sites were observed in the telomeric region of the short arm of chromosome 12 in 96% of the spreads, chromosomes 9 and 10 in 76% of the spreads, and chromosome 17 in 56% of the spreads.
文摘A 6kb rDNA probe comprising the 18S coding plusspacer sequences has been hybridized to the metaphase chromosomes of apple rootstock cultivar MM106 demon-strating the localization of ribosomal gene arrays in the vicinity of the telomeric regions of the short arms of chro-mosomes 6 and 14. The in situ results using digoxygenin labelling coupled to an alkaline phosphatase immunoassay were confirmed by silver staining for NORs and nucleoli. This study demonstrates the feasibility of molecular cyto-genetic analysis of very small chromosomes (1.0-2.7μm)of apple.
