Porcine colostrum was separated into the acid soluble fraction (SF) and casein fraction (CF) by acidifying followed by centrifuge. SF was further separated by liquid chromatography and anisotropic membrane filtration...Porcine colostrum was separated into the acid soluble fraction (SF) and casein fraction (CF) by acidifying followed by centrifuge. SF was further separated by liquid chromatography and anisotropic membrane filtration. Capacities of the SF or CF of porcine colostrum, to inhibit trypsin and chymotrypsin activity and to inhibit the epidermal growth factor (EGF) degradation in pig small intestinal contents, were determined under different heat treatments. The study showed that trypsin inhibitors in porcine colostrum survived heat treatments of 100℃ water bath for up to 10 min, but exposure to boiling water bath for 30 min significantly decreased the inhibitory activity. Compared with the trypsin inhibitors, the chymotrypsin inhibitors were more heat sensitive. SF was more heat sensitive than CF. Separation of the SF of porcine colostrum by liquid chromatography and anisotropic membrane filtration revealed that the porcine colostrum protease inhibitors, those had the capacity to inhibit the trypsin chymotrypsin activity and enhanced the stability of EGF in the gastrointestinal(GI) lumen of weaned pigs, existed mainly in SF, milk derived, were a group of heat labile small proteins with molecular weight of 10 00050 000.展开更多
Porcine colostrum and milk were separated into the acid-soluble fraction(SF)and casein fraction(CF)by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components were deter...Porcine colostrum and milk were separated into the acid-soluble fraction(SF)and casein fraction(CF)by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components were determined by incubating bovine trypsin or chymotrypsin in a medium. The inhibition of insulin-like growth factor I(IGF-I)and epidermal growth factor(EGF)degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid(TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The SF was higher in inhibitory potency than CF. The present study revealed that the protease inhibitors in porcine colostrum, milk-derived and colostrum-specific, existed mainly in SF.展开更多
object To explore the effect of hMLHland hMSH2 in carcinogenesis and progression of colorectal carcinoma,and their influences on proliferation in colorectal adenoma and malignant change in adenomas, Methods we investi...object To explore the effect of hMLHland hMSH2 in carcinogenesis and progression of colorectal carcinoma,and their influences on proliferation in colorectal adenoma and malignant change in adenomas, Methods we investigated the expression of mismatch repair (MMR） gene hMLH1, hMSH2 and proliferation cell nuclear antigen (PCNA)by immunohistochemistry in 63 cases colorectal adenomas,20cases malignant change in adenomas and 20ceses colorectal carcinomas (CBCs). Results The positive rate of hMLH1 and hMSH2 in colorectal adenomas,malignant change in adenomas and CBCs were significantly lower than that in normal mucosa. With increasing dysplasia in adenomas, the expression rate of hMLH1 and hMSH2 protein decreased gradually; The PCNA label index of tumors with overexpression hMSH2 were significantly higher than that of tumors with negative hMSH2 in colorectal malignant change type in adenomas and CRCs. PCNA lable index of tumors with overexpression hMSH2 were significant higher than that of tumors with negative hMSH2 in adenomas with grade Ⅱ, Ⅲ dysplasia. The changes of PCNA label index in colorectal adenomas, malignant change type in adenomas and CBCs were not association with the expression of hMLH1. Conclusion It indicated that the MMB genes mutation and abnormal function of DNA mismatch repair maybe participate in carcinogenesis of CBCs. It is might be an early event in carcinogenesis of CBCs. It suggested that the reduction of proliferating activities in colorectal neoplasms might be related to mutation and defect of MMB gene.展开更多
In the teaching of high school geography, whether it is physical geography or human geography, no matter how the new curriculum is reformed, leaving the map teaching is equivalent to "flying out of the sky",...In the teaching of high school geography, whether it is physical geography or human geography, no matter how the new curriculum is reformed, leaving the map teaching is equivalent to "flying out of the sky", for this reason in the high school geography teaching, only by organically and effectively integrating the map into the teaching, giving full play to the various functions of the map, can we cultivate students' spatial concept and comprehensive analysis ability, cultivate students' "seeing, analyzing, drawing, and recalling" skills on the map, and cultivate students' cognitive ability to map, so as to effectively help students observe geographical phenomena and analyze geographical problems. Understand geographical knowledge, effectively display the characteristic special effects of the map, and greatly improve the auxiliary role of the map.展开更多
One kind of unique continuation property for a wave equation is discussed. The authors show that, if one classical solution of the wave equation vanishes in an open set on a hyperplane, then it must vanish in a larger...One kind of unique continuation property for a wave equation is discussed. The authors show that, if one classical solution of the wave equation vanishes in an open set on a hyperplane, then it must vanish in a larger set on this hyperplane. The result can be viewed as a localized version of Robbiano’s result[9]. The approach involves the localized Fourier-Gauss transformation and unique continuation on a line in the Laplace equation.展开更多
文摘Porcine colostrum was separated into the acid soluble fraction (SF) and casein fraction (CF) by acidifying followed by centrifuge. SF was further separated by liquid chromatography and anisotropic membrane filtration. Capacities of the SF or CF of porcine colostrum, to inhibit trypsin and chymotrypsin activity and to inhibit the epidermal growth factor (EGF) degradation in pig small intestinal contents, were determined under different heat treatments. The study showed that trypsin inhibitors in porcine colostrum survived heat treatments of 100℃ water bath for up to 10 min, but exposure to boiling water bath for 30 min significantly decreased the inhibitory activity. Compared with the trypsin inhibitors, the chymotrypsin inhibitors were more heat sensitive. SF was more heat sensitive than CF. Separation of the SF of porcine colostrum by liquid chromatography and anisotropic membrane filtration revealed that the porcine colostrum protease inhibitors, those had the capacity to inhibit the trypsin chymotrypsin activity and enhanced the stability of EGF in the gastrointestinal(GI) lumen of weaned pigs, existed mainly in SF, milk derived, were a group of heat labile small proteins with molecular weight of 10 00050 000.
文摘Porcine colostrum and milk were separated into the acid-soluble fraction(SF)and casein fraction(CF)by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components were determined by incubating bovine trypsin or chymotrypsin in a medium. The inhibition of insulin-like growth factor I(IGF-I)and epidermal growth factor(EGF)degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid(TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The SF was higher in inhibitory potency than CF. The present study revealed that the protease inhibitors in porcine colostrum, milk-derived and colostrum-specific, existed mainly in SF.
文摘object To explore the effect of hMLHland hMSH2 in carcinogenesis and progression of colorectal carcinoma,and their influences on proliferation in colorectal adenoma and malignant change in adenomas, Methods we investigated the expression of mismatch repair (MMR） gene hMLH1, hMSH2 and proliferation cell nuclear antigen (PCNA)by immunohistochemistry in 63 cases colorectal adenomas,20cases malignant change in adenomas and 20ceses colorectal carcinomas (CBCs). Results The positive rate of hMLH1 and hMSH2 in colorectal adenomas,malignant change in adenomas and CBCs were significantly lower than that in normal mucosa. With increasing dysplasia in adenomas, the expression rate of hMLH1 and hMSH2 protein decreased gradually; The PCNA label index of tumors with overexpression hMSH2 were significantly higher than that of tumors with negative hMSH2 in colorectal malignant change type in adenomas and CRCs. PCNA lable index of tumors with overexpression hMSH2 were significant higher than that of tumors with negative hMSH2 in adenomas with grade Ⅱ, Ⅲ dysplasia. The changes of PCNA label index in colorectal adenomas, malignant change type in adenomas and CBCs were not association with the expression of hMLH1. Conclusion It indicated that the MMB genes mutation and abnormal function of DNA mismatch repair maybe participate in carcinogenesis of CBCs. It is might be an early event in carcinogenesis of CBCs. It suggested that the reduction of proliferating activities in colorectal neoplasms might be related to mutation and defect of MMB gene.
文摘In the teaching of high school geography, whether it is physical geography or human geography, no matter how the new curriculum is reformed, leaving the map teaching is equivalent to "flying out of the sky", for this reason in the high school geography teaching, only by organically and effectively integrating the map into the teaching, giving full play to the various functions of the map, can we cultivate students' spatial concept and comprehensive analysis ability, cultivate students' "seeing, analyzing, drawing, and recalling" skills on the map, and cultivate students' cognitive ability to map, so as to effectively help students observe geographical phenomena and analyze geographical problems. Understand geographical knowledge, effectively display the characteristic special effects of the map, and greatly improve the auxiliary role of the map.
文摘One kind of unique continuation property for a wave equation is discussed. The authors show that, if one classical solution of the wave equation vanishes in an open set on a hyperplane, then it must vanish in a larger set on this hyperplane. The result can be viewed as a localized version of Robbiano’s result[9]. The approach involves the localized Fourier-Gauss transformation and unique continuation on a line in the Laplace equation.
