Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into thre...Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups After 30day experiment, myocardial histopathological observation was performed. Location and distribution of NFκB p50 was examined by immunohistochemical assay. Expression of NFκB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NFκB; Myocardium p53 gene expression was examined by RTPCR analysis Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group Compared with control group, there were many myocardium nucleuses, which expressed NFκB p50 and distribute under epicardium Expression of NFκB p50 protein in nucleus increased significantly in ADR group The NFκB binding activity increased significantly in ADR group Myocardium expressions of p53 mRNA increased in ADR group Conclusions The NFκB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NFκB plays an important role in causing degeneration of myocardial tissue and regulating expression of relatedapoptosis genes展开更多
文摘Background This study aimed at investigating the change and significance of nuclear factorκB (NFκB) in cardiomyopathy induced by adriamycin (ADR) in ratsMethods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups After 30day experiment, myocardial histopathological observation was performed. Location and distribution of NFκB p50 was examined by immunohistochemical assay. Expression of NFκB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NFκB; Myocardium p53 gene expression was examined by RTPCR analysis Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group Compared with control group, there were many myocardium nucleuses, which expressed NFκB p50 and distribute under epicardium Expression of NFκB p50 protein in nucleus increased significantly in ADR group The NFκB binding activity increased significantly in ADR group Myocardium expressions of p53 mRNA increased in ADR group Conclusions The NFκB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NFκB plays an important role in causing degeneration of myocardial tissue and regulating expression of relatedapoptosis genes
