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西红花实时荧光定量PCR内参基因筛选 被引量:5
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作者 周琳 蔡友铭 +3 位作者 杨柳燕 赵冰雪 李芳 张永春 《分子植物育种》 CAS CSCD 北大核心 2020年第22期7467-7475,共9页
为筛选西红花不同组织间稳定表达的最佳内参基因,以西红花的根、球茎、主芽、花丝、叶片、侧芽共6个组织为试验材料,运用实时荧光定量PCR方法分析了18S核糖体RNA (18S rRNA)、肌动蛋白基因(ACT)、转录延伸因子-1α(EF1α)、3-磷酸-甘油... 为筛选西红花不同组织间稳定表达的最佳内参基因,以西红花的根、球茎、主芽、花丝、叶片、侧芽共6个组织为试验材料,运用实时荧光定量PCR方法分析了18S核糖体RNA (18S rRNA)、肌动蛋白基因(ACT)、转录延伸因子-1α(EF1α)、3-磷酸-甘油醛脱氢酶基因(GAPDH)、微管蛋白基因(TUB)和多聚泛素基因(UBQ)共6个内参基因在不同组织中的表达差异情况。基于Ct值,利用geNorm、NormFinder和BestKeeper分析6个内参基因表达稳定性。结果表明,3个软件筛选出的最佳内参基因略有不同。综合分析结果显示,GAPDH和UBQ表达较为稳定,其次为18S rRNA和ACT,而EF1α和TUB不适合作为西红花不同组织的内参基因。本研究确定西红花q RT-PCR分析的合适内参基因为GAPDH和UBQ,可用于种球发育、花发育、次生代谢产物形成和积累等机理研究。 展开更多
关键词 西红花(Crocus sativus) 不同组织 内参基因 实时荧光定量PCR
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一种基于PatchMatch的多视影像密集匹配改进方法 被引量:3
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作者 张雪 邓非 +1 位作者 周琳 常越 《测绘地理信息》 2019年第4期90-93,共4页
多视密集匹配是倾斜摄影测量中的研究热点,提出一种基于改进的PatchMatch多视密集匹配算法。算法采用自适应归一化互相关作为匹配代价,由PatchMatch算法迭代运算优化得到影像深度图,再基于可见性融合深度图得到场景的三维点云。该方法... 多视密集匹配是倾斜摄影测量中的研究热点,提出一种基于改进的PatchMatch多视密集匹配算法。算法采用自适应归一化互相关作为匹配代价,由PatchMatch算法迭代运算优化得到影像深度图,再基于可见性融合深度图得到场景的三维点云。该方法在匹配所需影像的选择上,采用了同时考虑视图层和像素层的影像选择方法,在测度选择上,提出了具有边缘特性的相似性测度。最后,对影像进行密集匹配试验,结果表明,产生的点云质量得到一定的改善,验证了该方法的有效性。 展开更多
关键词 三维重建 多视密集匹配 PatchMatch 归一化互相关 深度图
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对苯二甲醚绿色合成工艺研究 被引量:2
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作者 张永富 孙娜 +3 位作者 周林 袁世申 牛鹏程 杨丰科 《山东化工》 CAS 2021年第10期58-59,62,共3页
采用无毒无害的绿色化工原料碳酸二甲酯(DMC)代替有毒有害的硫酸二甲酯、碘甲烷、氯甲烷等作为对苯二酚(HQ)的甲基化试剂,合成对苯二甲醚,并从催化剂种类、催化剂用量、反应物配比、反应温度、反应时间等方面进行优化研究。结果表明:最... 采用无毒无害的绿色化工原料碳酸二甲酯(DMC)代替有毒有害的硫酸二甲酯、碘甲烷、氯甲烷等作为对苯二酚(HQ)的甲基化试剂,合成对苯二甲醚,并从催化剂种类、催化剂用量、反应物配比、反应温度、反应时间等方面进行优化研究。结果表明:最佳工艺条件为:n(HQ)∶n(DMC)=1∶4,催化剂为DBN(1,5-二氮杂二环[4.3.0]壬-5-烯),催化剂用量为HQ用量的10%,反应温度160℃,反应时间6 h。在该条件下对苯二甲醚的收率为98.6%,且反应无废水和废盐产生,实现绿色合成目标。 展开更多
关键词 对苯二酚 对苯二甲醚 碳酸二甲酯 绿色合成
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Natural Mongolian Pine Forests in the Great Xing'an Mountains,Northeast China 被引量:4
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作者 ZhouZhiqiang LiuTong zhoulin 《Forestry Studies in China》 CAS 2003年第3期1-5,共5页
Based upon 134 rel関es, the vegetation of the forests with Mongolian pine (Pinus sylvestris var. mongolica) of the Great Xingan Mountains in the northeast China was classified into 3 communities, 7 types and 4 subtype... Based upon 134 rel関es, the vegetation of the forests with Mongolian pine (Pinus sylvestris var. mongolica) of the Great Xingan Mountains in the northeast China was classified into 3 communities, 7 types and 4 subtypes by numerical cluster analysis. 1) The Pinus sylvestris var. mongolica-Populus davidiana-community was found on relatively dry sites. It was subdivided into a Quercus mongolica-type, consisting of a Adenophora tetraphylla subtype, a Pulsatilla dahurica subtype, and a pure type; 2) The Pinus sylvestris var. mongolica-Rhododendron dauricum-community occurred on medium dry sites. It included a Calamagrostis angustifolia type, consisting of a Cladonia arbuscula subtype, a pure subtype, and a Rubus idaeus-type; 3) The Pinus sylvestris var. mongolica-Ledum palustre-community was found on periodically wet sites. It included a Picea koraiensis type, a Vaccinium uliginosum type, and a pure type. 展开更多
关键词 Great Xingan Mountains northeast China PINE Pinus sylvestris var. mongolica forest VEGETATION numerical classification
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The Research on the Accuracy and Value of Clinical Blood Lipid Biochemical Test by Grading Test
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作者 ZHANGTing CUIYanfang +2 位作者 LILi LIANGYan zhoulin 《外文科技期刊数据库(文摘版)医药卫生》 2022年第6期211-214,共4页
Objective: to analyze the test accuracy and clinical value of the biochemical test of clinical serum lipids. Methods: a total of 88 patients underwent blood lipid test in the clinical laboratory department of our hosp... Objective: to analyze the test accuracy and clinical value of the biochemical test of clinical serum lipids. Methods: a total of 88 patients underwent blood lipid test in the clinical laboratory department of our hospital from January 2021 to January 2022.Classification test and drag-test are used to compare the accuracy of test results and compare the test cost. Results: the positive rate of the test result is different from the test method, and the data is meaningful (P <0.05);the missing rate is lower than the test-test, the data is meaningful (P <0.05);the test cost is lower than the conventional test, and the data is meaningful (t=23.785, P=0.000).Conclusion: for the clinical biochemical testing of blood lipid, the testing accuracy of the graded test method is higher, which can meet the clinical needs, and can reduce the testing cost, and can be promoted. 展开更多
关键词 blood lipid biochemical test grading test CHOLESTEROL leakage rate
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Practical Study on Promoting the Learning of "Contemporary Culture Participation Task Group" with Community Activities —— Taking Longnan No.1 Middle School in Gansu Province as an Example
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作者 zhoulin 《外文科技期刊数据库(文摘版)教育科学》 2022年第8期078-082,共5页
This study has great teaching value and is suitable for a variety of language learning activities. However, at present, there are few studies on the "contemporary cultural participation task group" in the fi... This study has great teaching value and is suitable for a variety of language learning activities. However, at present, there are few studies on the "contemporary cultural participation task group" in the field of Chinese education research. This paper expounds the teaching practice strategy of "contemporary culture participation task group" from three perspectives of "creating the situation, showing the contemporary real life", "based on words, excavating the hidden language flavor" and "studying the contemporary cultural phenomenon based on culture". The learning task group provides a good background for language transmission, which can stimulate students enthusiasm for language decoding and language coding, make students willing to invest in Chinese learning, and effectively experience the application of Chinese learning, so that the pragmatic into practice. 展开更多
关键词 contemporary cultural participation task group Chinese language activities practice strategy
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