Background Cotton is an industrial crop renowned for its multifaceted applications in the textiles,pharmaceuticals,and biofuel industries.Plant regeneration through somatic embryogenesis(SE)plays a crucial role in the...Background Cotton is an industrial crop renowned for its multifaceted applications in the textiles,pharmaceuticals,and biofuel industries.Plant regeneration through somatic embryogenesis(SE)plays a crucial role in the genetic improvement of cotton.There is a strong correlation between SE and zygotic embryogenesis(ZE)in plants.Furthermore,the strategy of ectopic expression of cotton genes into the model plant Arabidopsis has been a widely accepted approach for functional study.Result Based on previous spatial transcriptomics of cotton somatic embryos,two genes,Gh HAT5 and Gh CRK29,were identified.They are highly expressed in cotyledon and epidermal cells of cotton cotyledonary embryos,respectively.In this study,Gh HAT5 and Gh CRK29 were ectopically expressed in Arabidopsis to investigate their functions.The result showed that in Arabidopsis zygotic embryos,the overexpression of Gh HAT5 promoted the development of apical embryonic upper-tier cells and embryonic cotyledon,while the overexpression of Gh CRK29 promoted the development of apical embryonic lower-tier cells and embryonic radicle.Given the similarities between somatic and zygotic embryogenesis,these findings suggest that Gh HAT5 and Gh CRK29 are involved in cotton SE.We also speculate that these genes may promote the expression of the Arabidopsis endogenous gene At SCR,which is crucial for embryonic development.Conclusion These results revealed that Gh HAT5 and Gh CRK29 regulate embryonic development and are essential in advancing our understanding of cotton SE and facilitating targeted genetic manipulation strategies to improve industrial crop traits and agricultural sustainability.展开更多
以均苯三甲酰肼(1,3,5-Benzenetricarbohydrazide,BTH)和对苯二甲醛(1,4-phthalaldehyde,PD)为有机单体构建模块,根据席夫碱(Schiff-base)反应原理和网状化学的设计思想,设计合成了一种新的腙键共价有机聚合物(BTH-PD-COP)。采用傅里叶...以均苯三甲酰肼(1,3,5-Benzenetricarbohydrazide,BTH)和对苯二甲醛(1,4-phthalaldehyde,PD)为有机单体构建模块,根据席夫碱(Schiff-base)反应原理和网状化学的设计思想,设计合成了一种新的腙键共价有机聚合物(BTH-PD-COP)。采用傅里叶变换红外光谱(FT-IR)、固体核磁(13 C NMR)、扫描电子显微镜(SEM)、X射线粉末衍射(XRD)、热重分析(TGA)等分别对BTH-PD-COP材料进行了结构和性能表征。结果表明,BTH-PD-COP材料具有优异的物理化学性能。展开更多
现有住宅建筑在室行为预测模型缺乏对住户差异性的合理考虑,导致模型往往存在整体预测精度不高和适用性受限等问题.针对这一问题,提出一种考虑住户差异性的马尔可夫链在室状态预测模型.该模型首先通过Spearman相关性分析确定了不同影响...现有住宅建筑在室行为预测模型缺乏对住户差异性的合理考虑,导致模型往往存在整体预测精度不高和适用性受限等问题.针对这一问题,提出一种考虑住户差异性的马尔可夫链在室状态预测模型.该模型首先通过Spearman相关性分析确定了不同影响因素(即特征参数)与住户总在室时长的相关性,将相关系数作为特征参数权值并结合聚类分析对住户群体进行分类.在此基础上采用马尔可夫链模型对住户在室状态进行预测.为评估所建立预测模型的性能,以英国TUS(Time Use Survey)数据库为例,将改进模型与传统马尔可夫链模型进行对比分析.结果表明,该方法能够综合考虑不同住户特征参数及其对在室行为的影响,对住户进行合理的分类,与传统马尔可夫模型相比,所建预测模型显著提升了整体性能,平均绝对误差和均方根误差分别减小了20.57%和15.35%.展开更多
基金supported by the National Key Research and Development Program of China(No.2022YFD1200300)。
文摘Background Cotton is an industrial crop renowned for its multifaceted applications in the textiles,pharmaceuticals,and biofuel industries.Plant regeneration through somatic embryogenesis(SE)plays a crucial role in the genetic improvement of cotton.There is a strong correlation between SE and zygotic embryogenesis(ZE)in plants.Furthermore,the strategy of ectopic expression of cotton genes into the model plant Arabidopsis has been a widely accepted approach for functional study.Result Based on previous spatial transcriptomics of cotton somatic embryos,two genes,Gh HAT5 and Gh CRK29,were identified.They are highly expressed in cotyledon and epidermal cells of cotton cotyledonary embryos,respectively.In this study,Gh HAT5 and Gh CRK29 were ectopically expressed in Arabidopsis to investigate their functions.The result showed that in Arabidopsis zygotic embryos,the overexpression of Gh HAT5 promoted the development of apical embryonic upper-tier cells and embryonic cotyledon,while the overexpression of Gh CRK29 promoted the development of apical embryonic lower-tier cells and embryonic radicle.Given the similarities between somatic and zygotic embryogenesis,these findings suggest that Gh HAT5 and Gh CRK29 are involved in cotton SE.We also speculate that these genes may promote the expression of the Arabidopsis endogenous gene At SCR,which is crucial for embryonic development.Conclusion These results revealed that Gh HAT5 and Gh CRK29 regulate embryonic development and are essential in advancing our understanding of cotton SE and facilitating targeted genetic manipulation strategies to improve industrial crop traits and agricultural sustainability.
文摘以均苯三甲酰肼(1,3,5-Benzenetricarbohydrazide,BTH)和对苯二甲醛(1,4-phthalaldehyde,PD)为有机单体构建模块,根据席夫碱(Schiff-base)反应原理和网状化学的设计思想,设计合成了一种新的腙键共价有机聚合物(BTH-PD-COP)。采用傅里叶变换红外光谱(FT-IR)、固体核磁(13 C NMR)、扫描电子显微镜(SEM)、X射线粉末衍射(XRD)、热重分析(TGA)等分别对BTH-PD-COP材料进行了结构和性能表征。结果表明,BTH-PD-COP材料具有优异的物理化学性能。
文摘现有住宅建筑在室行为预测模型缺乏对住户差异性的合理考虑,导致模型往往存在整体预测精度不高和适用性受限等问题.针对这一问题,提出一种考虑住户差异性的马尔可夫链在室状态预测模型.该模型首先通过Spearman相关性分析确定了不同影响因素(即特征参数)与住户总在室时长的相关性,将相关系数作为特征参数权值并结合聚类分析对住户群体进行分类.在此基础上采用马尔可夫链模型对住户在室状态进行预测.为评估所建立预测模型的性能,以英国TUS(Time Use Survey)数据库为例,将改进模型与传统马尔可夫链模型进行对比分析.结果表明,该方法能够综合考虑不同住户特征参数及其对在室行为的影响,对住户进行合理的分类,与传统马尔可夫模型相比,所建预测模型显著提升了整体性能,平均绝对误差和均方根误差分别减小了20.57%和15.35%.
