To explore the metabolites of Antarctic marine-derived fungus Penicillium crustosum HDN20-1061 by OSMAC strategy.Methods Multiple medium trials determined fermentation condition and various methods including ODS colum...To explore the metabolites of Antarctic marine-derived fungus Penicillium crustosum HDN20-1061 by OSMAC strategy.Methods Multiple medium trials determined fermentation condition and various methods including ODS column and chromatography and semi-preparative HPLC were employed to separate and purify the extracts of strain HDN20-1061.NMR,MS,ECD and NMR calculations were used to determine the structure of compounds.Results Two new compounds,namely peniphenone F(1)and peniphenone G(2),along with one known compound(3)were isolated from the Penicillium crustosum HDN20-1061.Compound 1 exhibited weak antibacterial activity with an MIC value of 64μM.展开更多
Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived end...Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).展开更多
文摘To explore the metabolites of Antarctic marine-derived fungus Penicillium crustosum HDN20-1061 by OSMAC strategy.Methods Multiple medium trials determined fermentation condition and various methods including ODS column and chromatography and semi-preparative HPLC were employed to separate and purify the extracts of strain HDN20-1061.NMR,MS,ECD and NMR calculations were used to determine the structure of compounds.Results Two new compounds,namely peniphenone F(1)and peniphenone G(2),along with one known compound(3)were isolated from the Penicillium crustosum HDN20-1061.Compound 1 exhibited weak antibacterial activity with an MIC value of 64μM.
基金supported by the National Natural Science Foundation of China(No.41806167)the High-Level Talents Research Fund of Qingdao Agricultural University(No.665/1120034)+4 种基金the NSFC-Shandong Joint Fund(No.U1906212)the Major Project of the 14th Five-Year Plan(No.2022QNLM030003-1)the Natural Science Foundation of Shandong Province(No.ZR2021ZD28)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.2021CXLH0012)the Youth Innovation Plan of Shandong Province(No.2019KJM004).
文摘Two new dihydropyrones,rhytismatones C(1)and D(2),and a known compound,penicillenol A1(3),were isolated from the co-culture broth of the deep-sea-derived fungus Penicillium crustosum PRB-2 and Suaeda salsa-derived endophytic fungus Peni-cillium citrinum HDN11-186.Their structures were elucidated through comprehensive analysis of nuclear magnetic resonance(NMR)spectra and mass spectra.The absolute configurations of new compounds were determined by calculating the electronic circular di-chroism(ECD)spectrum.UPLC-MS data showed that compounds 1–3 could only be detected in the media of co-culture,suggesting new biosynthetic pathways were activated in the co-cultured fungi.Compound 1 showed obvious antibacterial activities against Pro-teus sp.MMBC-1002 and Bacillus subtilis MMBC-1004 with minimum inhibitory concentration(MIC)both at 25μmolL^(-1).
