A strain saccharomycete STSM-1 with high flocculanting activity was isolated from activated sludge with conventional methods. The high production rate and the low cost STSM-1 medium was obtained by selecting different...A strain saccharomycete STSM-1 with high flocculanting activity was isolated from activated sludge with conventional methods. The high production rate and the low cost STSM-1 medium was obtained by selecting different kinds of media, carbon source, nitrogen source and inorganic salt ion. The best flocculant-producing conditions were found by changing medium initial pH, culture temperature and ventilation flow. The best flocculating effect was obtained by changing positive ion types, density and concentration of flocculant.展开更多
Conventional modal parameter identifications are usually based on frequencyresponse functions, which require measurements of both the input force and the resulting response.However, in many cases, only response data a...Conventional modal parameter identifications are usually based on frequencyresponse functions, which require measurements of both the input force and the resulting response.However, in many cases, only response data are available while the actual excitations (such aswind/wave load) are not measurable. Modal parameters estimation must base itself on response-onlydata. Over the past years, many time-domain modal parameter identification techniques fromoutput-only are proposed. A poly-reference frequency-domain modal identification scheme onresponse-only is presented. It is based on coupling the cross-correlation theory with conventionalfrequency-domain modal parameter extraction. An experiment using an airplane model is performed toverify the proposed method.展开更多
For some large-scale engineering structures in operating conditions, modal param- eters estimation must base itself on response-only data. This problem has received a considerable amount of attention in the past few y...For some large-scale engineering structures in operating conditions, modal param- eters estimation must base itself on response-only data. This problem has received a considerable amount of attention in the past few years. It is well known that the cross-correlation function between the measured responses is a sum of complex exponential functions of the same form as the impulse response function of the original system. So this paper presents a time-domain operating modal identifcation global scheme and a frequency-domain scheme from output-only by cou- pling the cross-correlation function with conventional modal parameter estimation. The outlined techniques are applied to an airplane model to estimate modal parameters from response-only data.展开更多
Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural pro-tein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombi-nant vector...Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural pro-tein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombi-nant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV an-tibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a poten-tial candidate vaccine of FMDV.展开更多
Neural stem cell (NSC) is the progenitor of the neural system with the character of self-renew and hav-ing the potential to differentiate into all the phenotypes in the central nervous system (CNS). NSC may serve as a...Neural stem cell (NSC) is the progenitor of the neural system with the character of self-renew and hav-ing the potential to differentiate into all the phenotypes in the central nervous system (CNS). NSC may serve as a source of cell transplantation for the treatment of neurodegenerative diseases to replace degenerative neurons. In this study, NSCs derived from E12.5 rat mesencephalon were main-tained and expanded using a serum-free defined medium containing basic fibroblast growth factor (bFGF) and epi-dermal growth factor (EGF). While proliferating, the cells were immunoreactive for nestin and remained multipotent to generate neurons, astrocytes, and oligodendrocytes. After 15 times passage the total number of the cell expanded about 2.4×104 fold. Compared with untreated cultures, ascorbic acid (AA) treatment led to more dopaminergic (DAergic) differentitiation as indicated by the expression of tyrosine hydroxylase (TH). With the concentration increasing, more TH+ neurons were obtained. 100 mmol/L AA could lead to a increase more than 20-fold, and a concentration of 10 靘ol/L could lead to nearly 5-fold increase in TH+ cells. However, the ratio of TH+ cells was not improved any longer with the AA increasing above the concentration of 100 靘ol/L. The results demonstrate that expanded NSCs can be induced to differentiate into dopamine neurons in vitro, which can pro-vide enough cell population for the cell transplantation, as a main intervention for the neurodegenerative diseases such as Parkinson抯 disease.展开更多
文摘A strain saccharomycete STSM-1 with high flocculanting activity was isolated from activated sludge with conventional methods. The high production rate and the low cost STSM-1 medium was obtained by selecting different kinds of media, carbon source, nitrogen source and inorganic salt ion. The best flocculant-producing conditions were found by changing medium initial pH, culture temperature and ventilation flow. The best flocculating effect was obtained by changing positive ion types, density and concentration of flocculant.
基金This project is supported by Aeronautics Foundation (No. 1152059), Civil Aviation Foundation (No.1007-272) the 9-th Five Plan of the Aeronautical Industrial Corporation (No.62.2.2.1), China.
文摘Conventional modal parameter identifications are usually based on frequencyresponse functions, which require measurements of both the input force and the resulting response.However, in many cases, only response data are available while the actual excitations (such aswind/wave load) are not measurable. Modal parameters estimation must base itself on response-onlydata. Over the past years, many time-domain modal parameter identification techniques fromoutput-only are proposed. A poly-reference frequency-domain modal identification scheme onresponse-only is presented. It is based on coupling the cross-correlation theory with conventionalfrequency-domain modal parameter extraction. An experiment using an airplane model is performed toverify the proposed method.
基金Project supported by the National Natural Science Foundation of China(No.50205012),Aeronautics Foundation(No.01152059)and Civil Aviation Foundation(No.1007-272001).
文摘For some large-scale engineering structures in operating conditions, modal param- eters estimation must base itself on response-only data. This problem has received a considerable amount of attention in the past few years. It is well known that the cross-correlation function between the measured responses is a sum of complex exponential functions of the same form as the impulse response function of the original system. So this paper presents a time-domain operating modal identifcation global scheme and a frequency-domain scheme from output-only by cou- pling the cross-correlation function with conventional modal parameter estimation. The outlined techniques are applied to an airplane model to estimate modal parameters from response-only data.
文摘Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural pro-tein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombi-nant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV an-tibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a poten-tial candidate vaccine of FMDV.
基金supported by the State“863"High-Tech Project(Grant Nos.2002AA205051 and 2001 AA216l5l)the National Key Project for Basic Research of China(Grant No.001CB509906)the Project of Beijing Science Community(Grant No.020220010190).
文摘Neural stem cell (NSC) is the progenitor of the neural system with the character of self-renew and hav-ing the potential to differentiate into all the phenotypes in the central nervous system (CNS). NSC may serve as a source of cell transplantation for the treatment of neurodegenerative diseases to replace degenerative neurons. In this study, NSCs derived from E12.5 rat mesencephalon were main-tained and expanded using a serum-free defined medium containing basic fibroblast growth factor (bFGF) and epi-dermal growth factor (EGF). While proliferating, the cells were immunoreactive for nestin and remained multipotent to generate neurons, astrocytes, and oligodendrocytes. After 15 times passage the total number of the cell expanded about 2.4×104 fold. Compared with untreated cultures, ascorbic acid (AA) treatment led to more dopaminergic (DAergic) differentitiation as indicated by the expression of tyrosine hydroxylase (TH). With the concentration increasing, more TH+ neurons were obtained. 100 mmol/L AA could lead to a increase more than 20-fold, and a concentration of 10 靘ol/L could lead to nearly 5-fold increase in TH+ cells. However, the ratio of TH+ cells was not improved any longer with the AA increasing above the concentration of 100 靘ol/L. The results demonstrate that expanded NSCs can be induced to differentiate into dopamine neurons in vitro, which can pro-vide enough cell population for the cell transplantation, as a main intervention for the neurodegenerative diseases such as Parkinson抯 disease.
