Objective:To analyze the medication rules of ZHENG Yuling in the treatment of cervical cancer,and provide a basis for the clinical practice of Chinese medicine for the treatment of cervical cancer.Methods:The data of ...Objective:To analyze the medication rules of ZHENG Yuling in the treatment of cervical cancer,and provide a basis for the clinical practice of Chinese medicine for the treatment of cervical cancer.Methods:The data of patients with cervical cancer treated by ZHENG Yuling in the First Hospital Affliated to Henan Univer-sity of Chinese Medicine from March 26,2017 to January 5,2021 were collected and the medication rules were analyzed.Results:Totally 143 cases were included,and the most frequently used prescriptions were Wuling Powder(五苓散),Simiao Powder(四妙散),Danggui Shaoyao Powder(当归芍药散),Shuyu Pill(薯损丸),Qingxin Lianzi Decoetion(清心莲子饮),Bazhen Decoction(八珍汤),etc.With statistics of the efficacy,all Chinese materia medica were divided into 19 categories,and their properties were mainly warm,mild and cold;the flavors of medicine were mainly sweet,pungent and bitter;the meridian tropism mainly ineludes the lung,liver,spleen and stomach.After cluster analysis and factor analysis,15 common factors were extracted;through the analysis of association rules,14 groups of Chinese materia medica combinations with high frequency associ-ation strength were obtai ned.Conclusion:ZHENG Yuling believes that the pathogenesis of cervical cancer is not only related to disorder between Chong and conception Channels due to the intermingling of dampness,tox-icity,blood stasis,and phlegm but also retention of urine due to qi stagnation and water retention.The direct disease location is in the uterus and indirect location may involve the lung,liver,spleen and kidney according to the development of disease,accompanying symptoms and the different constitutions of the patients.It is advo-cated that attention should be paid to not only removing dampness,detoxification,removing blood stasis,and re-ducing phlegm,but also regulating qi,opening the blockage,promoting water flow.At the same time,the spleen and stomach should be protected,and tonification and purgation should be applied in combination.展开更多
目的基于网络药理学和动物实验探讨酸枣仁-远志药对治疗乳腺癌相关性失眠(breast cancer related insomnia,BCRI)的作用机制。方法通过TCMSP数据库、HERB数据库、DisGeNET数据库筛选酸枣仁-远志药对治疗BCRI的核心靶点;STRING数据库构...目的基于网络药理学和动物实验探讨酸枣仁-远志药对治疗乳腺癌相关性失眠(breast cancer related insomnia,BCRI)的作用机制。方法通过TCMSP数据库、HERB数据库、DisGeNET数据库筛选酸枣仁-远志药对治疗BCRI的核心靶点;STRING数据库构建酸枣仁-远志药对治疗BCRI的核心靶点蛋白互作网络图;利用Metascape平台对核心靶点进行GO及KEGG富集分析。60只小鼠分为对照组、BCRI组、酸枣仁-远志药对组、地西泮组、酸枣仁-远志药对+LY294002(PI3K抑制剂)组,每组12只。对照组小鼠仅构建乳腺癌模型,不进行慢性不可预知刺激及腹腔注射环磷酰胺处理;其他组小鼠均通过乳腺癌模型+慢性不可预知刺激+腹腔注射环磷酰胺的方法构建BCRI模型,建模成功后给药,1次/d,持续7 d。检测小鼠睡眠潜伏期、睡眠持续时长;HE染色检测下丘脑病理;ELISA检测下丘脑中4-氨基丁酸(4-aminobutyric acid,GABA)、5-羟色胺(5-hydroxytryptamine,5-HT)水平;qRT-PCR检测下丘脑中脑和肌肉芳香烃受体核转运蛋白样蛋白-1(brain and muscle arnt-like 1,BMAL1)、昼夜运动输出周期蛋白(circadian locomotor output cycles kaput,CLOCK)mRNA表达;检测下丘脑中磷酸化磷脂酰肌醇3-激酶(phosphorylated phosphatidylinositol 3-kinase,p-PI3K)、磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)蛋白。结果酸枣仁-远志药对治疗BCRI的核心靶点分别为AKT1、TP53、TNF、ALB、HIF1A、STAT3、ESR1、BCL2、HSP90AA1、CASP3、PPARG、HSP90AB1、MAPK3、TGFB1、MMP9、MTOR、CCND1。对上述17个核心靶点进行GO富集分析得到酸枣仁-远志药对治疗BCRI的细胞组分(cellular component,CC)共23条,分子功能(molecular function,MF)共47条,生物过程(biological process,BP)共211条;KEGG富集分析得到116条通路,预测PI3K/AKT通路可能是酸枣仁-远志药对治疗BCRI的的重要机制。动物实验表明,与对照组相比,BCRI组小鼠下丘脑病理损伤严重,睡眠潜伏期延长,睡眠持续时长减少,下丘脑中GABA、5-HT水平,BMAL1、CLOCK mRNA表达及p-PI3K、p-AKT蛋白降低(P<0.05);与BCRI组相比,酸枣仁-远志药对组、地西泮组小鼠下丘脑病理损伤减轻,睡眠潜伏期缩短,睡眠持续时长增加,下丘脑中GABA、5-HT水平,BMAL1、CLOCK mRNA表达及p-PI3K、p-AKT蛋白升高(P<0.05);LY294002逆转了酸枣仁-远志药对对BCRI小鼠下丘脑病理、神经递质及昼夜节律的影响。结论酸枣仁-远志药对能改善BCRI小鼠下丘脑病理,促进神经递质平衡,恢复昼夜节律,进而改善睡眠,且其作用机制可能与调控P13K/AKT通路有关。展开更多
基金We thank for the funding support from the "HundredThousandTen Thousand"Talents Project of Chinese Medicine Inheritance and Innovation(Qihuang Project)—Qihuang Scholars(284 official letter of People′s Education of Chinese Medicine 2018)as well as Special Scientific Research Project of National Clinical Research Base of Traditional Chinese Medicine(2019JDZX001).
文摘Objective:To analyze the medication rules of ZHENG Yuling in the treatment of cervical cancer,and provide a basis for the clinical practice of Chinese medicine for the treatment of cervical cancer.Methods:The data of patients with cervical cancer treated by ZHENG Yuling in the First Hospital Affliated to Henan Univer-sity of Chinese Medicine from March 26,2017 to January 5,2021 were collected and the medication rules were analyzed.Results:Totally 143 cases were included,and the most frequently used prescriptions were Wuling Powder(五苓散),Simiao Powder(四妙散),Danggui Shaoyao Powder(当归芍药散),Shuyu Pill(薯损丸),Qingxin Lianzi Decoetion(清心莲子饮),Bazhen Decoction(八珍汤),etc.With statistics of the efficacy,all Chinese materia medica were divided into 19 categories,and their properties were mainly warm,mild and cold;the flavors of medicine were mainly sweet,pungent and bitter;the meridian tropism mainly ineludes the lung,liver,spleen and stomach.After cluster analysis and factor analysis,15 common factors were extracted;through the analysis of association rules,14 groups of Chinese materia medica combinations with high frequency associ-ation strength were obtai ned.Conclusion:ZHENG Yuling believes that the pathogenesis of cervical cancer is not only related to disorder between Chong and conception Channels due to the intermingling of dampness,tox-icity,blood stasis,and phlegm but also retention of urine due to qi stagnation and water retention.The direct disease location is in the uterus and indirect location may involve the lung,liver,spleen and kidney according to the development of disease,accompanying symptoms and the different constitutions of the patients.It is advo-cated that attention should be paid to not only removing dampness,detoxification,removing blood stasis,and re-ducing phlegm,but also regulating qi,opening the blockage,promoting water flow.At the same time,the spleen and stomach should be protected,and tonification and purgation should be applied in combination.
